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The multi-reflecting time-of-flight (MRT) platform provides mass resolution exceeding 200,000 Full Width at Half Maximum and sub-ppm mass accuracy, enabling detailed isotopic fine structure analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The performance of the MRT platforms in identifying lipids and metabolites was evaluated using selected serum samples from a group of hospitalized COVID-19 patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Lipids and polar metabolites were extracted from human serum samples from the Manchester Asthma, Allergy and Thoracic Surgery Biobank and analysed using the MRT platform with data-independent acquisition.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The MRT platform facilitated the resolution of overlapping lipid species and improved annotation confidence. In a pilot cohort (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098), metabolomic differences associated with disease severity were observed, alongside annotation of clinically relevant metabolites including sulphated bile acids and retained iohexol associated with renal dysfunction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings demonstrate that MRT platform enhances confidence in metabolite identification and supports high-throughput metabolomics, offering significant potential for biomarker discovery in clinical studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11306-026-02539-w","type":"journal-article","created":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:14:57Z","timestamp":1791051297000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi-reflecting time-of-flight mass spectrometry enables high-accuracy metabolite identification in a demonstration study using serum samples from COVID-19 patients"],"prefix":"10.1007","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0819-2874","authenticated-orcid":false,"given":"Adam","family":"King","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9416-0835","authenticated-orcid":false,"given":"Eleanor","family":"Matthews","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1666-9377","authenticated-orcid":false,"given":"Garth","family":"Maker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2454-0477","authenticated-orcid":false,"given":"Robert","family":"Trengove","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7455-0166","authenticated-orcid":false,"given":"Shaufa","family":"Shareef","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4524-1663","authenticated-orcid":false,"given":"Stephen","family":"Fowler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6868-6633","authenticated-orcid":false,"given":"Timothy","family":"Felton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2733-6666","authenticated-orcid":false,"given":"Angela","family":"Simpson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1658-9334","authenticated-orcid":false,"given":"Martin","family":"Palmer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3513-364X","authenticated-orcid":false,"given":"Lee A.","family":"Gethings","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1380-9285","authenticated-orcid":false,"given":"Robert S.","family":"Plumb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7433-1740","authenticated-orcid":false,"given":"E. 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Analytical Chemistry, 85(11), 5288\u20135296. https:\/\/doi.org\/10.1021\/ac4001223","journal-title":"Analytical Chemistry"}],"container-title":["Metabolomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11306-026-02539-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11306-026-02539-w","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11306-026-02539-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:02:15Z","timestamp":1791871335000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11306-026-02539-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,3]]},"references-count":82,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2026,10]]}},"alternative-id":["2539"],"URL":"https:\/\/doi.org\/10.1007\/s11306-026-02539-w","relation":{},"ISSN":["1573-3890"],"issn-type":[{"value":"1573-3890","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,3]]},"assertion":[{"value":"12 June 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 September 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"3 October 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"A.K., R.P, L.A.G. and R.S.P. are employed by Waters Corporation, a manufacturer and vendor of mass spectrometers used for multiomics. All other authors report no conflict of interest.","order":1,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Patient samples were from the ManARTS Biobank, where patients provided their informed consent in accordance with the Human Tissue Authority and the National Research Ethics Service of the UK (ethical approval REC ref: M2020-92). All patient samples and associated clinical meta data were obtained according to the ethical approvals and were in accordance with the 1964 Helsinki Declaration and its later amendments.","order":2,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"168"}}