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In this study, we investigated the role of the MAPK pathway in wound healing in a murine model of emphysema using hyperpolarized\n                      <jats:sup>129<\/jats:sup>\n                      Xe (HP\n                      <jats:sup>129<\/jats:sup>\n                      Xe) magnetic resonance imaging (MRI).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Procedures<\/jats:title>\n                    <jats:p>\n                      Porcine pancreatic elastase was administered intratracheally to 25 mice to induce lung injury. Temporal changes in pulmonary gas exchange function were monitored using HP\n                      <jats:sup>129<\/jats:sup>\n                      Xe MRI, revealing a significant decline in function one day after elastase administration. Treatments with ethyl pyruvate (EP) and nicorandil (Nic), which upregulate and downregulate the MAPK pathway, respectively, were initiated in 12 and 7 of the 25 mice, respectively, and continued for 20\u00a0days. Over the 21-day period, HP\n                      <jats:sup>129<\/jats:sup>\n                      Xe MRI was performed to monitor the disease progression and treatment efficacy through changes in the metrics of gas exchange and fractional ventilation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      HP\n                      <jats:sup>129<\/jats:sup>\n                      Xe MRI showed that EP significantly improved gas exchange function 14\u00a0days after elastase administration, whereas Nic did not show any improvement. Ventilatory function also improved in the EP group, but not in the Nic group, 14\u00a0days after elastase administration. Histological analysis showed that EP repaired tissue damage to a level similar to that observed in healthy mice, whereas Nic did not.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      In the present study, we provide some insight into the role of the MAPK pathway in wound healing in elastase-induced lung injury, as assessed using the HP\n                      <jats:sup>129<\/jats:sup>\n                      Xe MRI protocol.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11307-025-02073-6","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T20:22:48Z","timestamp":1765398168000},"page":"169-180","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ethyl Pyruvate Promotes Wound Healing in Elastase-Induced Lung Injury in Mice as Assessed by Hyperpolarized 129Xe Magnetic Resonance Imaging"],"prefix":"10.1007","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5702-276X","authenticated-orcid":false,"given":"Atsuomi","family":"Kimura","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Shimokawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil J.","family":"Stewart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rie","family":"Hosoi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hirohiko","family":"Imai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideaki","family":"Fujiwara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,12,10]]},"reference":[{"key":"2073_CR1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics15040474","volume":"15","author":"JL MacLeod","year":"2025","unstructured":"MacLeod JL, Khan HM, Franklin A, Myc L, Shim YM (2025) Hyperpolarized xenon-129 MRI: narrative review of clinical studies, testing, and implementation of advanced pulmonary in vivo imaging and its diagnostic applications. 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