{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:00:53Z","timestamp":1787238053247,"version":"build-2736575974"},"reference-count":64,"publisher":"Springer Science and Business Media LLC","issue":"36","license":[{"start":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T00:00:00Z","timestamp":1647475200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T00:00:00Z","timestamp":1647475200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Environ Sci Pollut Res"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    (\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    ) is frequently detected in ruminants, especially dairy cattle, and associated with the sporadic and epidemic outbreak of listeriosis in farms. In this epidemiological study, the prevalence, virulence, antibiotic resistance profiles, and genetic diversity of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    in three Egyptian dairy cattle farms were investigated. The risk factors associated with the fecal shedding of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    were analyzed. The\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    strains from the three farms were categorized into distinct genotypes based on sampling site and sample type through enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR). A total of 1896 samples were collected from animals, environments, and milking equipment in the three farms. Results revealed that 137 (7.23%) of these samples were\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    positive. The prevalence of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    in the animal samples was high (32.1%), and the main environmental source of prevalent genotypes in the three farms was silage. For all sample types,\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    was more prevalent in farm I than in farms II and III. Risk factor analysis showed seasonal variation in production hygiene. For all sample types,\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    was significantly more prevalent in winter than in spring and summer. The level of\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    fecal shedding was high likely because of increasing age, number of parities, and milk yield in dairy cattle. Two virulence genes, namely,\n                    <jats:italic>hlyA<\/jats:italic>\n                    &amp;\n                    <jats:italic>prfA<\/jats:italic>\n                    , were also detected in 93 strains, whereas only one of these genes was found in 44 residual strains. Conversely,\n                    <jats:italic>iap<\/jats:italic>\n                    was completely absent in all strains. The strains exhibited phenotypic resistance to most of the tested antibiotics, but none of them was resistant to netilmicin or vancomycin. According to sample type, the strains from the animal samples were extremely resistant to amoxicillin (95.2%, 80\/84) and cloxacillin (92.9%, 78\/84). By comparison, the strains from the environmental samples were highly resistant to cefotaxime (86.95%, 20\/23). Furthermore, 25 multi-antibiotic resistance (MAR) patterns were observed in\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    strains\n                    <jats:italic>.<\/jats:italic>\n                    All strains had a MAR index of 0.22\u20130.78 and harbored antibiotic resistance genes, including extended-spectrum \u03b2-lactamase (\n                    <jats:italic>bla<\/jats:italic>\n                    CTX-M [92.7%] and\n                    <jats:italic>bla<\/jats:italic>\n                    DHA-1 [66.4%]), quinolones (\n                    <jats:italic>qnrS<\/jats:italic>\n                    [91.2%],\n                    <jats:italic>qnrA<\/jats:italic>\n                    [58.4%],\n                    <jats:italic>parC<\/jats:italic>\n                    [58.4%], and\n                    <jats:italic>qnrB<\/jats:italic>\n                    [51%]), macrolides (\n                    <jats:italic>erm<\/jats:italic>\n                    [B] [76.6%],\n                    <jats:italic>erm<\/jats:italic>\n                    (C) [1.5%], and\n                    <jats:italic>msr<\/jats:italic>\n                    (A) [27%]), trimethoprim (\n                    <jats:italic>dfrD<\/jats:italic>\n                    [65.7%]), and tetracyclines (\n                    <jats:italic>tet<\/jats:italic>\n                    (M) [41.6%],\n                    <jats:italic>tet<\/jats:italic>\n                    (S) [8%], and\n                    <jats:italic>int-Tn<\/jats:italic>\n                    [26.3%]). ERIC-PCR confirmed that the strains were genetically diverse and heterogeneous. A total of 137 isolated\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    strains were classified into 22 distinct ERIC-PCR groups (A\u2013V). Among them, ERIC E (10.2%) was the most prevalent group. These results indicated that environment and milking equipment served as reservoirs and potential transmission ways of virulent and multidrug-resistant\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    to dairy animals, consequently posing threats to public health. Silage is the main environmental source of prevalent genotypes on all three farms. Therefore, hygienic measures at the farm level should be developed and implemented to reduce\n                    <jats:italic>L. monocytogenes<\/jats:italic>\n                    transmission inside dairy cattle farms.\n                  <\/jats:p>","DOI":"10.1007\/s11356-022-19495-2","type":"journal-article","created":{"date-parts":[[2022,3,17]],"date-time":"2022-03-17T02:03:37Z","timestamp":1647482617000},"page":"54359-54377","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Epidemiological study on Listeria monocytogenes in Egyptian dairy cattle farms\u2019 insights into genetic diversity of multi-antibiotic-resistant strains by ERIC-PCR"],"prefix":"10.1007","volume":"29","author":[{"given":"Mona M.","family":"Elsayed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasha M.","family":"Elkenany","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira I.","family":"Zakaria","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basma M.","family":"Badawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,3,17]]},"reference":[{"key":"19495_CR1","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1016\/s0168-1605(97)01245-2","volume":"35","author":"A Agersborg","year":"1997","unstructured":"Agersborg A, Dahl R, Martinez I (1997) Sample preparation and DNA extraction procedures for polymerase chain reaction identification of Listeria monocytogenes in sea foods. Int J Food Microbiol 35:275\u2013280. https:\/\/doi.org\/10.1016\/s0168-1605(97)01245-2","journal-title":"Int J Food Microbiol"},{"key":"19495_CR2","volume-title":"Standard methods for examination of waters and wastewater","author":"American Public Health Association","year":"1971","unstructured":"American Public Health Association (1971) Standard methods for examination of waters and wastewater, 14th edn. D. C, Washington","edition":"14"},{"key":"19495_CR3","doi-asserted-by":"publisher","unstructured":"Bandelj P, Ciglenecki UJ, Ocepek M, Blagus R, Vengust M (2018) Risk factors associated with fecal shedding of Listeria monocytogenes by dairy cows and calves. Vet Intern Med 1\u20137. https:\/\/doi.org\/10.1111\/jvim.15234","DOI":"10.1111\/jvim.15234"},{"issue":"3","key":"19495_CR4","doi-asserted-by":"publisher","first-page":"570","DOI":"10.1111\/mmi.14454","volume":"113","author":"F Baquero","year":"2020","unstructured":"Baquero F, Lanza F, V, Duval M, Coque TM, (2020) Ecogenetics of antibiotic resistance in Listeria monocytogenes. Mol Microbiol 113(3):570\u2013579. https:\/\/doi.org\/10.1111\/mmi.14454","journal-title":"Mol Microbiol"},{"key":"19495_CR5","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1016\/j.foodcont.2013.01.004","volume":"32","author":"DM Bianchi","year":"2013","unstructured":"Bianchi DM, Barbaro A, Gallina S, Vitale N, Chiavacci L, Caramelli M, Decastelli L (2013) Monitoring of foodborne pathogenic bacteria in vending machine raw milk in Piedmont, Italy. Food Control 32:435\u2013439. https:\/\/doi.org\/10.1016\/j.foodcont.2013.01.004","journal-title":"Food Control"},{"key":"19495_CR6","doi-asserted-by":"publisher","unstructured":"Bilung LM, Chai LS, Tahar AS, Ted CK, Apun K (2018) Prevalence, genetic heterogeneity, and antibiotic resistance profile of Listeria spp. and Listeria monocytogenes at farm level: a highlight of ERIC- and BOX-PCR to reveal genetic diversity. Biomed Res Int 12. https:\/\/doi.org\/10.1155\/2018\/3067494","DOI":"10.1155\/2018\/3067494"},{"key":"19495_CR7","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1016\/0923-2508(92)90019-k","volume":"143","author":"M Bohnert","year":"1992","unstructured":"Bohnert M, Dilasser F, Dalet C, Mengaud J, Cossart P (1992) Use of specific oligonucleotides for direct enumeration of Listeria monocytogenes in food samples by colony hybridization and rapid detection by PCR. Res Microbiol 143:271\u2013280. https:\/\/doi.org\/10.1016\/0923-2508(92)90019-k","journal-title":"Res Microbiol"},{"key":"19495_CR8","doi-asserted-by":"publisher","first-page":"379","DOI":"10.4161\/viru.2.5.17703","volume":"2","author":"A Camejo","year":"2011","unstructured":"Camejo A, Carvalho F, Reis O, Leit\u00e3o E, Sousa S, Cabanes D (2011) The arsenal of virulence factors deployed by Listeria monocytogenes to promote its cell infection cycle. Virulence 2:379\u2013394. https:\/\/doi.org\/10.4161\/viru.2.5.17703","journal-title":"Virulence"},{"key":"19495_CR9","doi-asserted-by":"publisher","first-page":"e02000","DOI":"10.1128\/aem.02000-17","volume":"84","author":"H Castro","year":"2018","unstructured":"Castro H, Jaakkonen A, Hakkinen M, Korkeala H, Lindstr\u00f6m M (2018) Occurrence, persistence, and contamination routes of Listeria monocytogenes genotypes on three Finnish dairy cattle farms: a longitudinal study. Appl Environ Microbiol 84:e02000-e2017. https:\/\/doi.org\/10.1128\/aem.02000-17","journal-title":"Appl Environ Microbiol"},{"issue":"9","key":"19495_CR10","doi-asserted-by":"publisher","first-page":"2103","DOI":"10.1128\/AAC.43.9.2103","volume":"43","author":"E Charpentier","year":"1999","unstructured":"Charpentier E, Courvalin P (1999) Antibiotic resistance in Listeria spp. Antimicrob Agents Chemother 43(9):2103\u20132108. https:\/\/doi.org\/10.1128\/AAC.43.9.2103","journal-title":"Antimicrob Agents Chemother"},{"issue":"4","key":"19495_CR11","doi-asserted-by":"publisher","first-page":"4561","DOI":"10.3168\/jds.2020-18885","volume":"104","author":"JTH Chow","year":"2021","unstructured":"Chow JTH, Gall AR, Johnson AK, Huynh TN (2021) Characterization of Listeria monocytogenes isolates from lactating dairy cows in a Wisconsin farm: antibiotic resistance, mammalian cell infection, and effects on the fecal microbiota. J Dairy Sci 104(4):4561\u20134574. https:\/\/doi.org\/10.3168\/jds.2020-18885","journal-title":"J Dairy Sci"},{"key":"19495_CR12","doi-asserted-by":"publisher","unstructured":"Clegg FG, Chiejina SN, Duncan AL, Kay RN, Wray C (1983) Outbreak of Salmonella Newport infection in dairy herds and their relationship to management and contamination of the environment. Vet Rec 112. https:\/\/doi.org\/10.1136\/vr.112.25.580","DOI":"10.1136\/vr.112.25.580"},{"key":"19495_CR13","unstructured":"Clinical and laboratory standards institute, CLSI (2020) Performance standards for antimicrobial susceptibility testing; Thirty Informational Supplement, 950 West Valley Road, Suite 1400 Wayne, PA 19087 USA."},{"key":"19495_CR14","unstructured":"Constable PD, Hinchcliff KW, Done SH, Gruenberg W (2016) Mastitis. Chapter 20. in: Veterinary medicine: a textbook of the diseases of cattle, horses, sheep, pigs and goats. Elsevier Health Sciences, Amsterdam, the Netherlands, pp. 2113\u20132208."},{"key":"19495_CR15","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1016\/j.foodcont.2015.08.019","volume":"60","author":"E Dalzini","year":"2016","unstructured":"Dalzini E, Bernini V, Bertasi B, Daminelli P, Losio MN, Varisco G (2016) Survey of prevalence and seasonal variability of Listeria monocytogenes in raw cow milk from northern Italy. Food Control 60:466\u2013470. https:\/\/doi.org\/10.1016\/j.foodcont.2015.08.019","journal-title":"Food Control"},{"key":"19495_CR16","doi-asserted-by":"publisher","unstructured":"Elbar S, Elkenany R, Elhadidy M, Younis G (2020) Prevalence, virulence and antibiotic susceptibility of Listeria monocytogenes isolated from sheep.\u00a0Mansoura Veterinary Med J 21(2): 48\u201352. https:\/\/doi.org\/10.35943\/mvmj.2020.21.2.207","DOI":"10.35943\/mvmj.2020.21.2.207"},{"issue":"6","key":"19495_CR17","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1089\/fpd.2016.2248","volume":"14","author":"C Escolar","year":"2017","unstructured":"Escolar C, G\u00f3mez D, del Carmen Rota Garc\u00eda M, Conchello P, Herrera A (2017) Antimicrobial resistance profiles of Listeria monocytogenes and Listeria innocua isolated from ready-to-eat products of animal origin in Spain. Foodborne pathogens and disease 14(6):357\u2013363. https:\/\/doi.org\/10.1089\/fpd.2016.2248","journal-title":"Foodborne pathogens and disease"},{"key":"19495_CR18","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1016\/j.foodcont.2008.09.010","volume":"20","author":"A Germini","year":"2009","unstructured":"Germini A, Masola A, Carnevali P, Marchelli R (2009) Simultaneous detection of Escherichia coli O175:H7, Salmonella spp., and Listeria monocytogenes by multiplex PCR. Food Control 20:733\u2013738. https:\/\/doi.org\/10.1016\/j.foodcont.2008.09.010","journal-title":"Food Control"},{"key":"19495_CR19","doi-asserted-by":"publisher","first-page":"704","DOI":"10.1128\/AAC.47.2.704-708.2003","volume":"47","author":"S Godreuil","year":"2003","unstructured":"Godreuil S, Galimand M, Gerbaud G, Jacquet C, Courvalin P (2003) Efflux pump Lde is associated with fluoroquinolone resistance in Listeria monocytogenes. Antimicrob Agents Chemother 47:704\u2013708. https:\/\/doi.org\/10.1128\/AAC.47.2.704-708.2003","journal-title":"Antimicrob Agents Chemother"},{"issue":"8","key":"19495_CR20","doi-asserted-by":"publisher","first-page":"2788","DOI":"10.1128\/AEM.01381-10","volume":"77","author":"SA Granier","year":"2011","unstructured":"Granier SA, Moubareck C, Colaneri C, Lemire A, Roussel S, Dao TT, Courvalin P, Brisabois A (2011) Antimicrobial resistance of Listeria monocytogenes isolates from food and the environment in France over a 10-year period. Appl Environ Microbiol 77(8):2788\u20132790. https:\/\/doi.org\/10.1128\/AEM.01381-10","journal-title":"Appl Environ Microbiol"},{"issue":"10","key":"19495_CR21","doi-asserted-by":"publisher","first-page":"2361","DOI":"10.1093\/jac\/dks244","volume":"67","author":"Q Guo","year":"2012","unstructured":"Guo Q, Wang P, Ma Y, Yang Y, Ye X, Wang M (2012) Co-production of SFO-1 and DHA-1 \u03b2-lactamases and 16S rRNA methylase ArmA in clinical isolates of Klebsiella pneumoniae. J Antimicrob Chemother 67(10):2361\u20132366. https:\/\/doi.org\/10.1093\/jac\/dks244","journal-title":"J Antimicrob Chemother"},{"key":"19495_CR22","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/S0167-5877(01)00207-0","volume":"51","author":"L Hassan","year":"2001","unstructured":"Hassan L, Mohammed HO, McDonough PL (2001) Farm-management and milking practices associated with the presence of Listeria monocytogenes in New York state dairy herds. Prev Vet Med 51:63\u201373. https:\/\/doi.org\/10.1016\/S0167-5877(01)00207-0","journal-title":"Prev Vet Med"},{"key":"19495_CR23","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/j.prevetmed.2007.03.005","volume":"80","author":"AJ Ho","year":"2007","unstructured":"Ho AJ, Ivanek R, Gr\u00f6hn YT, Nightingale KK, Wiedmann M (2007) Listeria monocytogenes fecal shedding in dairy cattle shows high levels of day-to-day variation and includes outbreaks and sporadic cases of shedding of specific L. monocytogenes subtypes. Prev Vet Med 80:287\u2013305. https:\/\/doi.org\/10.1016\/j.prevetmed.2007.03.005","journal-title":"Prev Vet Med"},{"key":"19495_CR24","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1111\/nyas.12830","volume":"1354","author":"DC Hooper","year":"2016","unstructured":"Hooper DC, Jacoby GA (2016) Mechanisms of drug resistance: quinolone resistance. Ann N Y Acad Sci 1354:12\u201331. https:\/\/doi.org\/10.1111\/nyas.12830","journal-title":"Ann N Y Acad Sci"},{"key":"19495_CR25","doi-asserted-by":"publisher","first-page":"1903","DOI":"10.1128\/jcm.28.9.1903-1905.1990","volume":"28","author":"PR Hunter","year":"1990","unstructured":"Hunter PR (1990) Reproducibility and indices of discriminatory power of microbial typing methods. J Clin Microbiol 28:1903\u20131905. https:\/\/doi.org\/10.1128\/jcm.28.9.1903-1905.1990","journal-title":"J Clin Microbiol"},{"issue":"2","key":"19495_CR26","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0228956","volume":"15","author":"CD Iwu","year":"2020","unstructured":"Iwu CD, Okoh AI (2020) Characterization of antibiogram fingerprints in Listeria monocytogenes recovered from irrigation water and agricultural soil samples. PLoS ONE 15(2):e0228956. https:\/\/doi.org\/10.1371\/journal.pone.0228956","journal-title":"PLoS ONE"},{"issue":"1","key":"19495_CR27","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1128\/JCM.37.1.103-109.1999","volume":"37","author":"B Jersek","year":"1999","unstructured":"Jersek B, Gilot P, Gubina M, Klun N, Mehle J, Tcherneva E, Rijpens N, Herman L (1999) Typing of Listeria monocytogenes strains by repetitive element sequence-based PCR. J Clin Microbiol 37(1):103\u2013109. https:\/\/doi.org\/10.1128\/JCM.37.1.103-109.1999","journal-title":"J Clin Microbiol"},{"key":"19495_CR28","doi-asserted-by":"publisher","first-page":"373","DOI":"10.3389\/fenvs.2021.737435","volume":"12","author":"A Kayode","year":"2021","unstructured":"Kayode A, Semerjian L, Osaili T, Olapade O, Okoh A (2021) Occurrence of multidrug-resistant Listeria monocytogenes in environmental waters: a menace of environmental and public health concern. Front Environ Sci 12:373. https:\/\/doi.org\/10.3389\/fenvs.2021.737435","journal-title":"Front Environ Sci"},{"key":"19495_CR29","doi-asserted-by":"publisher","first-page":"2792","DOI":"10.3168\/jds.2009-2717","volume":"93","author":"AA Latorre","year":"2010","unstructured":"Latorre AA et al (2010) Biofilm in milking equipment on a dairy farm as a potential source of bulk tank milk contamination with Listeria monocytogenes. J Dairy Sci 93:2792\u20132802. https:\/\/doi.org\/10.3168\/jds.2009-2717","journal-title":"J Dairy Sci"},{"key":"19495_CR30","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1086\/324626","volume":"34","author":"R Leclercq","year":"2002","unstructured":"Leclercq R (2002) Mechanisms of resistance to macrolides and lincosamides: nature of the resistance elements and their clinical implications. Clin Infect Dis 34:482\u2013492. https:\/\/doi.org\/10.1086\/324626","journal-title":"Clin Infect Dis"},{"issue":"4","key":"19495_CR31","doi-asserted-by":"publisher","first-page":"1646","DOI":"10.1128\/aac.49.4.1646-1648.2005","volume":"49","author":"R Leclercq","year":"2005","unstructured":"Leclercq R, Huet C, Picherot M, Trieu-Cuot P, Poyart C (2005) Genetic basis of antibiotic resistance in clinical isolates of Streptococcus gallolyticus (Streptococcus bovis). Antimicrob Agents Chemother 49(4):1646\u20131648. https:\/\/doi.org\/10.1128\/aac.49.4.1646-1648.2005","journal-title":"Antimicrob Agents Chemother"},{"key":"19495_CR32","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.mimet.2007.08.007","volume":"71","author":"D Liu","year":"2007","unstructured":"Liu D, Lawrence ML, Austin FW, Ainsworth AJ (2007) A multiplex PCR for species-and virulence-specific determination of Listeria monocytogenes. J Microbiol Methods 71:133\u2013140. https:\/\/doi.org\/10.1016\/j.mimet.2007.08.007","journal-title":"J Microbiol Methods"},{"key":"19495_CR33","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.mimet.2007.08.007","volume":"101","author":"DM Livermore","year":"1996","unstructured":"Livermore DM (1996) Are all beta-lactams created equal? Scandinavian journal of infectious diseases. Supplementum 101:33\u201343. https:\/\/doi.org\/10.1016\/j.mimet.2007.08.007","journal-title":"Supplementum"},{"key":"19495_CR34","doi-asserted-by":"publisher","unstructured":"L\u00f3pe M, Tenorio C, Del Campo R, Zarazaga M, Torres C (2011) Characterization of the mechanisms of fluoroquinolone resistance in vancomycin-resistant enterococci of different origins. J Chemother 23(2):87\u201391. https:\/\/doi.org\/10.1179\/joc.2011.23.2.87","DOI":"10.1179\/joc.2011.23.2.87"},{"key":"19495_CR35","doi-asserted-by":"publisher","first-page":"302","DOI":"10.3923\/ajava.2017.302.310","volume":"12","author":"AN Mohammed","year":"2017","unstructured":"Mohammed AN, Abdel Aziz SA (2017) Ecological study on Listeria monocytogenes and the extent of its resistance to different disinfectants in dairy farm for improving animal health. Asian Journal of Animal and Veterinary Advances 12:302\u2013310. https:\/\/doi.org\/10.3923\/ajava.2017.302.310","journal-title":"Asian Journal of Animal and Veterinary Advances"},{"key":"19495_CR36","doi-asserted-by":"publisher","first-page":"2728","DOI":"10.1128\/AAC.01557-09","volume":"54","author":"A Morvan","year":"2010","unstructured":"Morvan A, Moubareck C, Leclercq A, Herv\u00e9-Bazin M, Bremont S, Lecuit M, Courvalin P, Le Monnier A (2010) Antimicrobial resistance of Listeria monocytogenes strains isolated from humans in France. Antimicrob Agents Chemother 54:2728\u20132731. https:\/\/doi.org\/10.1128\/AAC.01557-09","journal-title":"Antimicrob Agents Chemother"},{"issue":"6","key":"19495_CR37","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1016\/j.ijantimicag.2006.03.007","volume":"27","author":"T Muratani","year":"2006","unstructured":"Muratani T, Kobayashi T, Matsumoto T (2006) Emergence and prevalence of \u03b2-lactamase-producing Klebsiella pneumoniae resistant to cephems in Japan. Int J Antimicrob Agents 27(6):491\u2013499. https:\/\/doi.org\/10.1016\/j.ijantimicag.2006.03.007","journal-title":"Int J Antimicrob Agents"},{"key":"19495_CR38","doi-asserted-by":"publisher","first-page":"1808","DOI":"10.2460\/javma.2005.227.1808","volume":"227","author":"KK Nightingale","year":"2005","unstructured":"Nightingale KK, Fortes ED, Ho AJ, Schukken YH, Gr\u00f6hn YT, Wiedmann M (2005) Evaluation of farm management practices as risk factors for clinical listeriosis and fecal shedding of Listeria monocytogenes in ruminants. J Am Vet Med Assoc 227:1808\u20131814. https:\/\/doi.org\/10.2460\/javma.2005.227.1808","journal-title":"J Am Vet Med Assoc"},{"issue":"8","key":"19495_CR39","doi-asserted-by":"publisher","first-page":"4458","DOI":"10.1128\/AEM.70.8.4458-4467.2004","volume":"70","author":"KK Nightingale","year":"2004","unstructured":"Nightingale KK, Schukken YH, Nightingale CR, Fortes ED, Ho AJ, Her Z, Grohn YT, McDonough PL, Wiedmann M (2004) Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment. Appl Environ Microbiol 70(8):4458\u20134467. https:\/\/doi.org\/10.1128\/AEM.70.8.4458-4467.2004","journal-title":"Appl Environ Microbiol"},{"key":"19495_CR40","doi-asserted-by":"publisher","unstructured":"Ojdana D, Sacha P, Wieczorek P, Czaban S, Michalska A, Jaworowska J (2014) The occurrence of blaCTX-M, blaSHV, and blaTEM genes in extended-spectrum \u03b2-lactamase-positive strains of Klebsiella pneumoniae, Escherichia coli, and Proteus mirabilis in Poland. Intern J Antibi 7. https:\/\/doi.org\/10.1155\/2014\/935842","DOI":"10.1155\/2014\/935842"},{"issue":"5","key":"19495_CR41","first-page":"512","volume":"32","author":"JCF Pantoja","year":"2012","unstructured":"Pantoja JCF, Rodrigues ACO, Hulland C, Reinemann DJ, Ruegg PL (2012) Investigating contamination of bulk tank milk with Listeria monocytogenes on a dairy farm. Food Prot Trends 32(5):512\u2013521","journal-title":"Food Prot Trends"},{"key":"19495_CR42","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1186\/s12866-019-1676-3","volume":"19","author":"B Papi\u0107","year":"2019","unstructured":"Papi\u0107 B, Pate M, F\u00e9lix B, Ku\u0161ar D (2019) Genetic diversity of Listeria monocytogenes strains in ruminant abortion and rhombencephalitis cases in comparison with the natural environment. BMC Microbiol 19:299. https:\/\/doi.org\/10.1186\/s12866-019-1676-3","journal-title":"BMC Microbiol"},{"key":"19495_CR43","doi-asserted-by":"publisher","unstructured":"Parsaie Mehr V, Shokoohizadeh L, Mirzaee M, Savari M (2017) Molecular typing of Klebsiella pneumoniae isolates by enterobacterial repetitive intergenic consensus (ERIC)\u2013PCR. Infect Epidemiol Microbiol 3(4): 112\u2013116. https:\/\/doi.org\/10.18869\/modares.iem.3.4.112","DOI":"10.18869\/modares.iem.3.4.112"},{"key":"19495_CR44","doi-asserted-by":"publisher","first-page":"1103","DOI":"10.3389\/fmicb.2018.01103","volume":"9","author":"SV Poimenidou","year":"2018","unstructured":"Poimenidou SV, Dalmasso M, Papadimitriou K, Fox EM, Skandamis PN, Jordan K (2018) Virulence gene sequencing highlights similarities and differences in sequences in Listeria monocytogenes serotype 1\/2a and 4b strains of clinical and food origin from 3 different geographic locations. Front Microbiol 9:1103. https:\/\/doi.org\/10.3389\/fmicb.2018.01103","journal-title":"Front Microbiol"},{"issue":"8","key":"19495_CR45","doi-asserted-by":"publisher","first-page":"2425","DOI":"10.1002\/j.1460-2075.1989.tb08373.x","volume":"8","author":"C Poyart-Salmeron","year":"1989","unstructured":"Poyart-Salmeron C, Trieu-Cuot P, Carlier C, Courvalin P (1989) Molecular characterization of two proteins involved in the excision of the conjugative transposon Tn1545: homologies with other site-specific recombinases. EMBO J 8(8):2425\u20132433. https:\/\/doi.org\/10.1002\/j.1460-2075.1989.tb08373.x","journal-title":"EMBO J"},{"key":"19495_CR46","doi-asserted-by":"publisher","first-page":"3900","DOI":"10.1128\/aem.71.7.3900-3910.2005","volume":"71","author":"A Roberts","year":"2005","unstructured":"Roberts A, Chan Y, Wiedmann M (2005) Definition of genetically distinct attenuation mechanisms in naturally virulence attenuated Listeria monocytogenes by comparative cell culture and molecular characterization. Appl Environ Microbiol 71:3900\u20133910. https:\/\/doi.org\/10.1128\/aem.71.7.3900-3910.2005","journal-title":"Appl Environ Microbiol"},{"key":"19495_CR47","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s00018-003-2225-6","volume":"60","author":"AJ Roberts","year":"2003","unstructured":"Roberts AJ, Wiedmann M (2003) Pathogen, host and environmental factors contributing to the pathogenesis of listeriosis. Cell Mol Life Sci 60:1\u201315. https:\/\/doi.org\/10.1007\/s00018-003-2225-6","journal-title":"Cell Mol Life Sci"},{"key":"19495_CR48","doi-asserted-by":"publisher","DOI":"10.1201\/9781420015188","volume-title":"Listeria, listeriosis, and food safety","author":"ET Ryser","year":"2007","unstructured":"Ryser ET, Marth EM (2007) Listeria, listeriosis, and food safety, 3rd edn. Taylor & Francis Group, Boca Raton","edition":"3"},{"key":"19495_CR49","volume-title":"Bovine mastitis","author":"OW Scha","year":"1971","unstructured":"Scha OW, Carrol E, Jain NC (1971) Bovine mastitis, 1st edn. Lea and Febiger, Philadelphia","edition":"1"},{"issue":"4","key":"19495_CR50","first-page":"241","volume":"20","author":"E Shamloo","year":"2019","unstructured":"Shamloo E, Hosseini H, Abdi Moghadam Z, Halberg Larsen M, Haslberger A, Alebouyeh M (2019) Importance of Listeria monocytogenes in food safety: a review of its prevalence, detection, and antibiotic resistance. Iran J Vet Res 20(4):241\u2013254","journal-title":"Iran J Vet Res"},{"issue":"8","key":"19495_CR51","doi-asserted-by":"publisher","first-page":"2027","DOI":"10.1016\/j.foodres.2010.06.001","volume":"43","author":"S Singh","year":"2010","unstructured":"Singh S, Yadav A, Singh S, Bharti P (2010) Prevalence of Salmonella in chicken eggs collected from poultry farms and marketing channels and their antimicrobial resistance. Food Res Int 43(8):2027\u20132030. https:\/\/doi.org\/10.1016\/j.foodres.2010.06.001","journal-title":"Food Res Int"},{"key":"19495_CR52","doi-asserted-by":"publisher","first-page":"2057","DOI":"10.5897\/AJMR12.1463","volume":"7","author":"R Sohrabi","year":"2013","unstructured":"Sohrabi R, Rashedi M, Dorcheh MP, Shahbazi AM (2013) Prevalence of Listeria species in raw milk in Esfahan Province. Iran Afr J Microbiol Res 7:2057\u20132060. https:\/\/doi.org\/10.5897\/AJMR12.1463","journal-title":"Iran Afr J Microbiol Res"},{"key":"19495_CR53","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1186\/s12866-014-0241-3","volume":"14","author":"DK Soni","year":"2014","unstructured":"Soni DK, Singh M, Singh DV, Dubey SK (2014) Virulence and genotypic characterization of Listeria monocytogenes isolated from vegetable and soil samples. BMC Microbiol 14:241. https:\/\/doi.org\/10.1186\/s12866-014-0241-3","journal-title":"BMC Microbiol"},{"key":"19495_CR54","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1016\/j.foodcont.2016.04.020","volume":"70","author":"X Su","year":"2016","unstructured":"Su X, Zhang S, Shi W, Yang X, Li Y, Pan H, Kuang D, Xu X, Shi X, Meng J (2016) Molecular characterization and antimicrobial susceptibility of Listeria monocytogenes isolated from foods and humans. Food Control 70:96\u2013102. https:\/\/doi.org\/10.1016\/j.foodcont.2016.04.020","journal-title":"Food Control"},{"key":"19495_CR55","doi-asserted-by":"publisher","unstructured":"Swetha CS, Porteen K, Elango A, Ronald BSM, Kumar TS, Milton AP, Sureshkannan S (2021) Genetic diversity, virulence and distribution of antimicrobial resistance among Listeria monocytogenes isolated from milk, beef, and bovine farm environment. Iran J Vet Res 22(1):1\u20138. https:\/\/doi.org\/10.22099\/ijvr.2020.37618.5472","DOI":"10.22099\/ijvr.2020.37618.5472"},{"key":"19495_CR56","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/j.jgar.2017.07.008","volume":"10","author":"AB Tahoun","year":"2017","unstructured":"Tahoun AB, Abou Elez RM, Abdelfatah EN, Elsohaby I, El-Gedawy AA, Elmoslemany AM (2017) Listeria monocytogenes in raw milk, milking equipment and dairy workers: molecular characterization and antimicrobial resistance patterns. Journal of Global Antimicrobial Resistance 10:264\u2013270. https:\/\/doi.org\/10.1016\/j.jgar.2017.07.008","journal-title":"Journal of Global Antimicrobial Resistance"},{"issue":"7","key":"19495_CR57","doi-asserted-by":"publisher","first-page":"851","DOI":"10.3390\/pathogens10070851","volume":"10","author":"M Terentjeva","year":"2021","unstructured":"Terentjeva M, \u0160teingolde \u017d, Meister I, Elferts D, Avsejenko J, Streiki\u0161a M, Gradovska S, Alksne L, Kibilds J, B\u0113rzi\u0146\u0161 A (2021) Prevalence, genetic diversity and factors associated with distribution of Listeria monocytogenes and other Listeria spp in cattle farms in Latvia. Pathogens 10(7):851. https:\/\/doi.org\/10.3390\/pathogens10070851","journal-title":"Pathogens"},{"key":"19495_CR58","doi-asserted-by":"publisher","first-page":"2822","DOI":"10.3168\/jds.s0022-0302(04)73410-4","volume":"87","author":"JS Van Kessel","year":"2004","unstructured":"Van Kessel JS, Karns JS, Gorski L, McCluskey BJ, Perdue ML (2004) Prevalence of Salmonellae, Listeria monocytogenes, and fecal coliforms in bulk tank milk on US dairies. J Dairy Sci 87:2822\u20132830. https:\/\/doi.org\/10.3168\/jds.s0022-0302(04)73410-4","journal-title":"J Dairy Sci"},{"key":"19495_CR59","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1128\/CMR.14.3.584-640.2001","volume":"14","author":"JA Vazquez-Boland","year":"2001","unstructured":"Vazquez-Boland JA, Kuhn M, Berche P, Chakraborty T, Dominguez-Bernal G, Goebel W, Gonzalez-Zorn B, Wehland J, Kreft J (2001) Listeria pathogenesis and molecular virulence determinants. Clin Microbiol Rev 14:584\u2013640. https:\/\/doi.org\/10.1128\/CMR.14.3.584-640.2001","journal-title":"Clin Microbiol Rev"},{"issue":"24","key":"19495_CR60","doi-asserted-by":"publisher","first-page":"6823","DOI":"10.1093\/nar\/19.24.6823","volume":"19","author":"J Versalovic","year":"1991","unstructured":"Versalovic J, Koeuth T, Lupski JR (1991) Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res 19(24):6823\u20136831. https:\/\/doi.org\/10.1093\/nar\/19.24.6823","journal-title":"Nucleic Acids Res"},{"issue":"24","key":"19495_CR61","doi-asserted-by":"publisher","first-page":"8666","DOI":"10.1128\/AEM.01859-12","volume":"78","author":"K Vongkamjan","year":"2012","unstructured":"Vongkamjan K, Andrea MS, Henk C, Bakker D, Esther DF, Martin W (2012) Silage collected from dairy farms harbors an abundance of listeriaphages with considerable host range and genome size diversity. Appl Environ Microbiol 78(24):8666\u20138675. https:\/\/doi.org\/10.1128\/AEM.01859-12","journal-title":"Appl Environ Microbiol"},{"key":"19495_CR62","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1016\/j.ijfoodmicro.2010.10.015","volume":"144","author":"H Yan","year":"2010","unstructured":"Yan H, Neogi SB, Mo Z, Guan W, Shen Z, Zhang S, Li L, Yamasaki S, Shi L, Zhong N (2010) Prevalence and characterization of antimicrobial resistance of foodborne Listeria monocytogenes isolates in Hebei province of Northern China, 2005\u20132007. Int J Food Microbiol 144:310\u2013316. https:\/\/doi.org\/10.1016\/j.ijfoodmicro.2010.10.015","journal-title":"Int J Food Microbiol"},{"key":"19495_CR63","doi-asserted-by":"publisher","first-page":"2827","DOI":"10.1128\/aem.58.9.2827-2831.1992","volume":"58","author":"RF Wang","year":"1992","unstructured":"Wang RF, Cao WW, Johnson MG (1992) 16S rRNA-based probes and polymerase chain reaction method to detect Listeria monocytogenes cells added to foods. Appl Environ Microbiol 58:2827\u20132831. https:\/\/doi.org\/10.1128\/aem.58.9.2827-2831.1992","journal-title":"Appl Environ Microbiol"},{"issue":"1","key":"19495_CR64","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12866-015-0609-z","volume":"15","author":"H Zeitoun","year":"2015","unstructured":"Zeitoun H, Kassem M, Raafat D, AbouShlieb H, Fanaki N (2015) Microbiological testing of pharmaceuticals and cosmetics in Egypt. BMC Microbiol 15(1):1\u201313. https:\/\/doi.org\/10.1186\/s12866-015-0609-z","journal-title":"BMC Microbiol"}],"container-title":["Environmental Science and Pollution Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11356-022-19495-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11356-022-19495-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11356-022-19495-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,6]],"date-time":"2022-08-06T16:18:01Z","timestamp":1659802681000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11356-022-19495-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,17]]},"references-count":64,"journal-issue":{"issue":"36","published-print":{"date-parts":[[2022,8]]}},"alternative-id":["19495"],"URL":"https:\/\/doi.org\/10.1007\/s11356-022-19495-2","relation":{},"ISSN":["0944-1344","1614-7499"],"issn-type":[{"value":"0944-1344","type":"print"},{"value":"1614-7499","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,3,17]]},"assertion":[{"value":"13 September 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 February 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"17 March 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This protocol was performed by following the animal ethics guidelines and approved by Medical Research Ethics Committee of Mansoura University with code number (R\/75).","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"Not applicable.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}