{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T22:42:08Z","timestamp":1793745728750,"version":"4.6.1"},"reference-count":82,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T00:00:00Z","timestamp":1667779200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T00:00:00Z","timestamp":1667779200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100009169","name":"Assiut University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100009169","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Environ Sci Pollut Res"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Microplastic particles (MPs) are a common environmental pollutant easily ingested by fish in aquaculture. The current study evaluated the protective efficacies of some antioxidant, e.g., lycopene, citric acid, and chlorella, against the toxic effects of MP ingestion by\n                    <jats:italic>Clarias gariepinus<\/jats:italic>\n                    using histopathological biomarkers. Five experimental groups were established, a control group receiving only a standard diet, a group exposed to 500\u00a0mg\/kg MP concomitant with the standard diet, and three antioxidant groups exposed to MPs plus either lycopene (500\u00a0mg\/kg), citric acid (30\u00a0g\/kg), or chlorella (50\u00a0g\/kg) in the standard diet. After 15\u00a0days, fish were sacrificed for histological and histochemical examinations. Histological analysis of the kidney for group 2 (fed 500\u00a0mg\/kg MPs alone) revealed distributed tissue dissociation, regional glomerular hypertrophy or shrinkage, melanomacrophage accumulation, and expansion of Bowman\u2019s space, while liver tissue exhibited dilation and rupture of the central vein wall, hemorrhage, cytoplasmic vacuolation, and cellular necrosis or apoptosis. Fish exposed to MPs also exhibited connective tissue fiber accumulation around renal blood vessels, renal tubules, the central hepatic vein, hepatic blood sinusoids, and serosal, muscle, and submucosal layers of the intestine. In addition, MP exposure reduced carbohydrate (mainly glycogen) contents in the brush borders and basement membranes of renal tubules, glomeruli, and intestinal tissues as well as in the cytoplasm of hepatocytes. These signs of renal, hepatic, and intestinal histopathology were fully or partially reversed by dietary lycopene, chlorella, or citric acid. Enhancing dietary antioxidants is an effective strategy for preventing MP toxicity in\n                    <jats:italic>Clarias gariepinus<\/jats:italic>\n                    in aquaculture.\n                  <\/jats:p>","DOI":"10.1007\/s11356-022-23789-w","type":"journal-article","created":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T03:10:54Z","timestamp":1667790654000},"page":"24424-24440","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":60,"title":["Protective efficacy of dietary natural antioxidants on microplastic particles-induced histopathological lesions in African catfish (Clarias gariepinus)"],"prefix":"10.1007","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7487-4729","authenticated-orcid":false,"given":"Alaa El-Din H.","family":"Sayed","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mervat N.","family":"Hana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed","family":"Hamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hany M. R.","family":"Abdel-Latif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae-Seong","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamdy A. M.","family":"Soliman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,7]]},"reference":[{"key":"23789_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2021.129977","volume":"276","author":"S Abarghouei","year":"2021","unstructured":"Abarghouei S, Hedayati A, Raeisi M, Hadavand BS, Rezaei H, Abed-Elmdoust A (2021) Size-dependent effects of microplastic on uptake, immune system, related gene expression and histopathology of goldfish (Carassius auratus). Chemosphere 276:129977","journal-title":"Chemosphere"},{"issue":"15262","key":"23789_CR2","first-page":"15274","volume":"23","author":"MA Abass","year":"2016","unstructured":"Abass MA, Elkhateeb SA, Abd El-Baset SA, Kattaia AA, Mohamed EM, Atteia HH (2016) Lycopene ameliorates atrazine-induced oxidative damage in adrenal cortex of male rats by activation of the Nrf2\/HO-1 pathway. Environ Sci Pollut Res 23(15262):15274","journal-title":"Environ Sci Pollut Res"},{"issue":"5","key":"23789_CR3","doi-asserted-by":"publisher","first-page":"588","DOI":"10.1089\/jmf.2013.0065","volume":"17","author":"OM Abdel-Salam","year":"2014","unstructured":"Abdel-Salam OM, Youness ER, Mohammed NA, Youssef MM, Omara EA, Sleem AA (2014) Citric acid effects on brain and liver oxidative stress in lipopolysaccharide-treated mice. J Med Food 17(5):588\u2013598","journal-title":"J Med Food"},{"key":"23789_CR4","first-page":"1181","volume":"9","author":"OM Abdel-Salam","year":"2016","unstructured":"Abdel-Salam OM, Youness ER, Mohammed NA, Yassen NN, Khadrawy YA, El-Toukhy SE, Sleem AA (2016) Novel neuroprotective and hepatoprotective effects of citric acid in acute malathion intoxication. J Med Food 9:1181\u20131194","journal-title":"J Med Food"},{"key":"23789_CR5","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.marpolbul.2019.110795","volume":"151","author":"C Ahrendt","year":"2020","unstructured":"Ahrendt C, Perez-Venegas DJ, Urbina M, Gonzalez C, Echeveste P, Aldana M, Pulgar J, Galb\u00e1n-Malag\u00f3n C (2020) Microplastic ingestion cause intestinal lesions in the intertidal fish Girella laevifrons. Mar Pollut Bull 151:10","journal-title":"Mar Pollut Bull"},{"issue":"6","key":"23789_CR6","doi-asserted-by":"publisher","first-page":"1","DOI":"10.9734\/BJMMR\/2016\/27654","volume":"18","author":"B Akdemir","year":"2016","unstructured":"Akdemir B, Bahcecioglu IH, Tuzcu M, Orhan C, Ispiroglu M, Ozercan IH et al (2016) Effect of lycopene and genistein on hepatic inflammation and fibrosis in thioacetamide induced liver injury in rats. Br J Med Med Res 18(6):1\u201311","journal-title":"Br J Med Med Res"},{"key":"23789_CR7","doi-asserted-by":"publisher","first-page":"735612","DOI":"10.1016\/j.aquaculture.2020.735612","volume":"528","author":"WF Carneiro","year":"2020","unstructured":"Carneiro WF, Castro TFD, Orlando TM, Meurer F, de Jesus Paula DA Jesus, do Carmo Rodrigues Virote B, da Cunha Barreto Vianna AR, Murgas LDS (2020) Replacing fish meal by Chlorella sp meal: effects on zebrafish growth, reproductive performance, biochemical parameters and digestive enzymes. Aquaculture 528:735612","journal-title":"Aquaculture"},{"issue":"12","key":"23789_CR8","doi-asserted-by":"publisher","first-page":"3804","DOI":"10.1111\/are.13847","volume":"49","author":"Z Chen","year":"2018","unstructured":"Chen Z, Zhao S, Liu Y, Yang P, Ai Q, Zhang W et al (2018) Dietary citric acid supplementation\u00a0alleviates soybean meal\u2010induced intestinal oxidative damage and micro\u2010ecological imbalance in juvenile turbot,\u00a0Scophthalmus maximus L. Aquac Res 49(12):3804\u20133816","journal-title":"Aquac Res"},{"key":"23789_CR9","doi-asserted-by":"crossref","unstructured":"Chen J, Rao C, Yuan R, Sun D, Guo S, Li L, ... Cao X (2022) Long-term exposure to polyethylene microplastics and glyphosate interferes with the behavior, intestinal microbial homeostasis, and metabolites of the common carp (Cyprinus carpio L.).\u00a0Sci Total Environ 814: 152681","DOI":"10.1016\/j.scitotenv.2021.152681"},{"issue":"12","key":"23789_CR10","doi-asserted-by":"publisher","first-page":"2588","DOI":"10.1016\/j.marpolbul.2011.09.025","volume":"62","author":"M Cole","year":"2011","unstructured":"Cole M, Lindeque P, Halsband C, Galloway TS (2011) Microplastics as contaminants in the marine environment: a review. Mar Pollut Bull 62(12):2588\u20132597","journal-title":"Mar Pollut Bull"},{"issue":"10","key":"23789_CR11","doi-asserted-by":"publisher","first-page":"4582","DOI":"10.1039\/D0FO03277H","volume":"12","author":"XY Dai","year":"2021","unstructured":"Dai XY, Zhao Y, Ge J, Zhu SY, Li MZ, Talukder M, Li JL (2021) Lycopene attenuates di (2-ethylhexyl) phthalate-induced mitophagy in spleen by regulating the sirtuin3-mediated pathway. Food Funct 12(10):4582\u20134590","journal-title":"Food Funct"},{"key":"23789_CR12","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1016\/j.jhazmat.2019.121066","volume":"382","author":"AP da Costa Ara\u00fajo","year":"2020","unstructured":"da Costa Ara\u00fajo AP, de Melo NFS, de Oliveira Junior AG, Rodrigues FP, Fernandes T, de Andrade Vieira JE, Rocha TL, Malafaia G (2020) How much are microplastics harmful to the health of amphibians? A study with pristine polyethylene microplastics and Physalaemus cuvieri. J Hazard Mater 382:24","journal-title":"J Hazard Mater"},{"issue":"2","key":"23789_CR13","doi-asserted-by":"publisher","first-page":"532","DOI":"10.1016\/0003-9861(89)90467-0","volume":"274","author":"P Di Mascio","year":"1989","unstructured":"Di Mascio P, Kaiser S, Sies H (1989) Lycopene as the most efficient biological carotenoid singlet oxygen quencher. Arch Biochem Biophys 274(2):532\u2013538","journal-title":"Arch Biochem Biophys"},{"key":"23789_CR14","unstructured":"Drury RA, Wallington EA (1980) Carleton\u2019s histological techniques, 5th edn. Oxford University Press, New York, pp 195"},{"key":"23789_CR15","doi-asserted-by":"publisher","first-page":"1056","DOI":"10.3389\/fphys.2019.01056","volume":"10","author":"A El-Gawad","year":"2019","unstructured":"El-Gawad A, Eman A, Wang H-P, Yao H (2019) Diet supplemented with synthetic carotenoids: effects on growth performance and biochemical and immunological parameters of yellow perch (Perca flavescens). Front Physiol 10:1056","journal-title":"Front Physiol"},{"key":"23789_CR16","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1177\/0748233712448115","volume":"30","author":"MS El-Gerbed","year":"2014","unstructured":"El-Gerbed MS (2014) Protective effect of lycopene on deltamethrin-induced histological and ultrastructural changes in kidney tissue of rats. Toxicol Ind Health 30:160\u2013173","journal-title":"Toxicol Ind Health"},{"key":"23789_CR17","first-page":"111","volume":"7","author":"AMA El-Saad","year":"2011","unstructured":"El-Saad AMA (2011) Lycopene protects against deltamethrin induced oxidative renal dysfunction in rats. Egypt J Exper Biol (zool) 7:111\u2013121","journal-title":"Egypt J Exper Biol (zool)"},{"key":"23789_CR18","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/j.fsi.2017.07.006","volume":"68","author":"C Espinosa","year":"2017","unstructured":"Espinosa C, Cuesta A, Esteban M (2017) Effects of dietary polyvinylchloride microparticles on general health, immune status and expression of several genes related to stress in gilthead seabream (Sparus aurata L.). Fish Shellfish Immunol 68:251\u2013259","journal-title":"Fish Shellfish Immunol"},{"key":"23789_CR19","doi-asserted-by":"publisher","first-page":"574","DOI":"10.1016\/j.fsi.2019.10.072","volume":"95","author":"C Espinosa","year":"2019","unstructured":"Espinosa C, Esteban M, Cuesta A (2019) Dietary administration of PVC and PE microplastics produces histological damage, oxidative stress and immunoregulation in European sea bass (Dicentrarchus labrax L.). Fish Shellfish Immunol 95:574\u2013583","journal-title":"Fish Shellfish Immunol"},{"key":"23789_CR20","unstructured":"Gomaah S, El Naggar G (2004) Status of African catfish (Clarias gariepinus) aquaculture around the world: a review, Proceedings of the first International Conference for Veterinary Research Division, National Research Center"},{"key":"23789_CR21","doi-asserted-by":"publisher","first-page":"120001","DOI":"10.1016\/j.envpol.2022.120001","volume":"311","author":"M Gonz\u00e1lez-Doncel","year":"2022","unstructured":"Gonz\u00e1lez-Doncel M, Garc\u00eda-Mauri\u00f1o JE, Beltr\u00e1n EM, Torija CF, Andreu-S\u00e1nchez O, Pablos MV (2022) Effects of life cycle exposure to polystyrene microplastics on medaka fish (Oryzias latipes). Environ Pollut 311:120001","journal-title":"Environ Pollut"},{"key":"23789_CR22","doi-asserted-by":"crossref","unstructured":"Guo X, Lv M, Li J, Ding J, Wang Y, Fu L, ... Chen L (2022) The distinct toxicity effects between commercial and realistic polystyrene microplastics on microbiome and histopathology of gut in zebrafish. J Hazard Mater 434: 128874","DOI":"10.1016\/j.jhazmat.2022.128874"},{"key":"23789_CR23","doi-asserted-by":"publisher","first-page":"20610","DOI":"10.1007\/s11356-019-05319-3","volume":"26","author":"M Hamed","year":"2019","unstructured":"Hamed M, Soliman HAM, Sayed AH (2019a) Ameliorative effect of Spirulina platensis against lead nitrate-induced cytotoxicity and genotoxicity in catfish Clarias gariepinus. Environ Sci Pollut Res Int 26:20610\u201320618","journal-title":"Environ Sci Pollut Res Int"},{"key":"23789_CR24","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1016\/j.chemosphere.2019.04.153","volume":"228","author":"M Hamed","year":"2019","unstructured":"Hamed M, Soliman HA, Osman AG, Sayed AEDH (2019b) Assessment the effect of exposure to microplastics in Nile Tilapia (Oreochromis niloticus) early juvenile: I. blood biomarkers. Chemosphere 228:345\u2013350","journal-title":"Chemosphere"},{"issue":"13","key":"23789_CR25","doi-asserted-by":"publisher","first-page":"14581","DOI":"10.1007\/s11356-020-07898-y","volume":"27","author":"M Hamed","year":"2020","unstructured":"Hamed M, Soliman HA, Osman AG, Sayed AEDH (2020) Antioxidants and molecular damage in Nile Tilapia (Oreochromis niloticus) after exposure to microplastics. Environ Sci Pollut Res 27(13):14581\u201314588","journal-title":"Environ Sci Pollut Res"},{"key":"23789_CR26","doi-asserted-by":"publisher","first-page":"149921","DOI":"10.1016\/j.scitotenv.2021.149921","volume":"803","author":"M Hamed","year":"2022","unstructured":"Hamed M, Monteiro CE, Sayed AE-DH (2022a) Investigation of the impact caused by different sizes of polyethylene plastics (nano, micro, and macro) in common carp juveniles, Cyprinus carpio L., using multi-biomarkers. Sci Total Environ 803:149921","journal-title":"Sci Total Environ"},{"key":"23789_CR27","doi-asserted-by":"publisher","first-page":"869727","DOI":"10.3389\/fenvs.2022.869727","volume":"10","author":"M Hamed","year":"2022","unstructured":"Hamed M, Soliman HAM, Eid Z, Al Naggar Y, Sayed AE-DH (2022b) Dietary feeding lycopene, citric acid, and Chlorella alleviated the neurotoxicity of polyethylene microplastics in African catfish (Clarias gariepinus). Front Environ Sci 10:869727. https:\/\/doi.org\/10.3389\/fenvs.2022.869727","journal-title":"Front Environ Sci"},{"key":"23789_CR28","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1007\/s13530-020-00042-w","volume":"12","author":"AMM Haredi","year":"2020","unstructured":"Haredi AMM, Mourad M, Tanekhy M, Wassif E, Abdel-Tawab HS (2020) Lake Edku pollutants induced biochemical and histopathological alterations in muscle tissues of Nile Tilapia (Oreochromis niloticus). Toxicol Environ Heal Sci 12:247\u2013255","journal-title":"Toxicol Environ Heal Sci"},{"key":"23789_CR29","doi-asserted-by":"publisher","first-page":"148490","DOI":"10.1016\/j.scitotenv.2021.148490","volume":"793","author":"N Hodkovicova","year":"2021","unstructured":"Hodkovicova N, Hollerova A, Caloudova H, Blahova J, Franc A, Garajova M et al (2021) Do foodborne polyethylene microparticles affect the health of rainbow trout (Oncorhynchus mykiss)? Sci Total Environ 793:148490","journal-title":"Sci Total Environ"},{"key":"23789_CR30","volume":"224","author":"MM Hussein","year":"2019","unstructured":"Hussein MM, Elsadaawy HA, El-Murr A, Ahmed MM, Bedawy AM, Tukur HA, Swelum AA-A, Saadeldin IM (2019) Endosulfan toxicity in Nile tilapia (Oreochromis niloticus) and the use of lycopene as an ameliorative agent. Comp Biochem Physiol c: Toxicol Pharmacol 224:108573","journal-title":"Comp Biochem Physiol c: Toxicol Pharmacol"},{"key":"23789_CR31","doi-asserted-by":"crossref","unstructured":"Hoseini SM, Khosraviani K, Delavar FH, Arghideh M, Zavvar F, Hoseinifar SH,\u2026 Reverter M (2022a) Hepatic transcriptomic and histopathological responses of common carp, Cyprinus carpio, to copper and microplastic exposure. Mar Pollut Bull 175:113401","DOI":"10.1016\/j.marpolbul.2022.113401"},{"key":"23789_CR32","doi-asserted-by":"crossref","unstructured":"Hoseini SM, Sinha R, Fazel A, Khosraviani K, HosseinpourDelavar F, Arghideh M,\u2026 Van Doan H (2022b) Histopathological damage and stress-and immune-related genes\u2019 expression in the intestine of common carp, Cyprinus carpio exposed to copper and polyvinyl chloride microparticle. J Exp Zool Pt A: Ecological and Integrative Physiology 337(2):181\u2013190","DOI":"10.1002\/jez.2555"},{"key":"23789_CR33","doi-asserted-by":"crossref","unstructured":"Hu J, Zuo J, Li J, Zhang Y, Ai X, Zhang J,\u2026 Sun D (2022) Effects of secondary polyethylene microplastic exposure on crucian (Carassius carassius) growth, liver damage, and gut microbiome composition. Sci Total Environ 802:149736","DOI":"10.1016\/j.scitotenv.2021.149736"},{"key":"23789_CR34","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/j.nutres.2013.03.006","volume":"33","author":"M Ichimura","year":"2013","unstructured":"Ichimura M, Kato S, Tsuneyama K, Matsutake S, Kamogawa M, Hirao E, Miyata A, Mori S, Yamaguchi N, Suruga K, Omagari K (2013) Phycocyanin prevents hypertension and low serum adiponectin level in a rat model of metabolic syndrome. Nutr Res 33:397\u2013405","journal-title":"Nutr Res"},{"key":"23789_CR35","doi-asserted-by":"publisher","first-page":"21159","DOI":"10.1007\/s11356-020-08611-9","volume":"27","author":"SC Iheanacho","year":"2020","unstructured":"Iheanacho SC, Odo GE (2020) Dietary exposure to polyvinyl chloride microparticles induced oxidative stress and hepatic damage in Clarias gariepinus (Burchell, 1822). Environ Sci Pollut Res 27:21159\u201321173","journal-title":"Environ Sci Pollut Res"},{"key":"23789_CR36","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/j.chemosphere.2018.09.031","volume":"213","author":"K Jabeen","year":"2018","unstructured":"Jabeen K, Li B, Chen Q, Su L, Wu C, Hollert H, Shi H (2018) Effects of virgin microplastics on goldfish (Carassius auratus). Chemosphere 213:323\u2013332","journal-title":"Chemosphere"},{"key":"23789_CR37","doi-asserted-by":"publisher","first-page":"535","DOI":"10.1016\/j.aaf.2020.07.017","volume":"6","author":"O Kaleem","year":"2021","unstructured":"Kaleem O, Sabi A-FBS (2021) Overview of aquaculture systems in Egypt and Nigeria, prospects, potentials, and constraints. Aquacul Fish 6:535\u2013547","journal-title":"Aquacul Fish"},{"key":"23789_CR38","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.envres.2016.07.024","volume":"151","author":"A Karami","year":"2016","unstructured":"Karami A, Romano N, Galloway T, Hamzah H (2016) Virgin microplastics cause toxicity and modulate the impacts of phenanthrene on biomarker responses in African catfish (Clarias gariepinus). Environ Res 151:58\u201370","journal-title":"Environ Res"},{"issue":"3","key":"23789_CR39","doi-asserted-by":"publisher","first-page":"1638","DOI":"10.1021\/es404295e","volume":"48","author":"KL Kaposi","year":"2014","unstructured":"Kaposi KL, Mos B, Kelaher BP, Dworjanyn SA (2014) Ingestion of microplastic has limited impact on a marine larva. Environ Sci Technol 48(3):1638\u20131645","journal-title":"Environ Sci Technol"},{"key":"23789_CR40","doi-asserted-by":"publisher","first-page":"118608","DOI":"10.1016\/j.watres.2022.118608","volume":"219","author":"H Liao","year":"2022","unstructured":"Liao H, Liu S, Junaid M, Gao D, Ai W, Chen G, Wang J (2022) Di-(2-ethylhexyl) phthalate exacerbated the toxicity of polystyrene nanoplastics through histological damage and intestinal microbiota dysbiosis in freshwater Micropterus salmoides. Water Res 219:118608","journal-title":"Water Res"},{"key":"23789_CR41","doi-asserted-by":"crossref","unstructured":"Liu Y, Jia X, Zhu H, Zhang Q, He Y, Shen Y, ... Li J (2022) The effects of exposure to microplastics on grass carp (Ctenopharyngodon idella) at the physiological, biochemical, and transcriptomic levels.\u00a0Chemosphere 286: 131831","DOI":"10.1016\/j.chemosphere.2021.131831"},{"key":"23789_CR42","doi-asserted-by":"crossref","unstructured":"Lu X, Deng DF, Huang F, Casu F, Kraco E, Newton RJ, ... Mendoza LMR (2022) Chronic exposure to high-density polyethylene microplastic through feeding alters the nutrient metabolism of juvenile yellow perch (Perca flavescens).\u00a0Anim Nutr 9: 143-158","DOI":"10.1016\/j.aninu.2022.01.007"},{"key":"23789_CR43","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.scitotenv.2017.11.103","volume":"620","author":"L Lei","year":"2018","unstructured":"Lei L, Wu S, Lu S, Liu M, Song Y, Fu Z, Shi H, Raley-Susman KM, He D (2018) Microplastic particles cause intestinal damage and other adverse effects in zebrafish Danio rerio and nematode Caenorhabditis elegans. Sci Total Environ 620:1\u20138","journal-title":"Sci Total Environ"},{"key":"23789_CR44","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.138662","volume":"727","author":"B Li","year":"2020","unstructured":"Li B, Su L, Zhang H, Deng H, Chen Q, Shi H (2020) Microplastics in fishes and their living environments surrounding a plastic production area. Sci Total Environ 727:138662","journal-title":"Sci Total Environ"},{"key":"23789_CR45","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-019-52292-5","volume":"9","author":"G Limonta","year":"2019","unstructured":"Limonta G, Mancia A, Benkhalqui A, Bertolucci C, Abelli L, Fossi MC, Panti C (2019) Microplastics induce transcriptional changes, immune response and behavioral alterations in adult zebrafish. Sci Rep 9:1\u201311","journal-title":"Sci Rep"},{"key":"23789_CR46","doi-asserted-by":"publisher","first-page":"4054","DOI":"10.1021\/acs.est.6b00183","volume":"50","author":"Y Lu","year":"2016","unstructured":"Lu Y, Zhang Y, Deng Y, Jiang W, Zhao Y, Geng J, Ding L, Ren H (2016) Uptake and accumulation of polystyrene microplastics in zebrafish (Danio rerio) and toxic effects in liver. Environ Sci Technol 50:4054\u20134060","journal-title":"Environ Sci Technol"},{"key":"23789_CR47","first-page":"35","volume":"9","author":"N Mahmoud","year":"2019","unstructured":"Mahmoud N, Eid A, Wahdan AA, Enany ME, El-Nab A, Asmaa S (2019) Effect of phytase and citric acid on growth performance, feed utilization and its antibacterial activity against fish pathogens of Nile tilapia fingerlings. Egypt J Aquat Res 9:35\u201353","journal-title":"Egypt J Aquat Res"},{"key":"23789_CR48","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2021.132631","volume":"288","author":"X Meng","year":"2022","unstructured":"Meng X, Zhang J, Wang W, Gonzalez-Gil G, Vrouwenvelder JS, Li Z (2022) Effects of nano-and microplastics on kidney: physicochemical properties, bioaccumulation, oxidative stress and immunoreaction. Chemosphere 288:132631","journal-title":"Chemosphere"},{"key":"23789_CR49","doi-asserted-by":"crossref","unstructured":"Montero D, Rimoldi S, Torrecillas S, Rapp J, Moroni F, Herrera A,\u2026 Terova G (2022) Impact of polypropylene microplastics and chemical pollutants on European sea bass (Dicentrarchus labrax) gut microbiota and health. Sci Total Environ 805:150402","DOI":"10.1016\/j.scitotenv.2021.150402"},{"key":"23789_CR50","doi-asserted-by":"publisher","first-page":"986","DOI":"10.37358\/RC.18.4.6242","volume":"69","author":"M Nicula","year":"2018","unstructured":"Nicula M, Pacala N, Stef L, Pet I, Dronca D, Ahmadi M, Gherbon A (2018) Garlic and chlorella biomodulate lead toxicity on manganese homeostasis in Carassius gibelio Bloch. Rev Chim 69:986\u2013989","journal-title":"Rev Chim"},{"key":"23789_CR51","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/j.envpol.2016.01.083","volume":"212","author":"C Ped\u00e0","year":"2016","unstructured":"Ped\u00e0 C, Caccamo L, Fossi MC, Gai F, Andaloro F, Genovese L, Perdichizzi A, Romeo T, Maricchiolo G (2016) Intestinal alterations in European sea bass Dicentrarchus labrax (Linnaeus, 1758) exposed to microplastics: preliminary results. Environ Pollut 212:251\u2013256","journal-title":"Environ Pollut"},{"key":"23789_CR52","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.chemosphere.2019.07.065","volume":"236","author":"R Qiao","year":"2019","unstructured":"Qiao R, Deng Y, Zhang S, Wolosker MB, Zhu Q, Ren H, Zhang Y (2019a) Accumulation of different shapes of microplastics initiates intestinal injury and gut microbiota dysbiosis in the gut of zebrafish. Chemosphere 236:10","journal-title":"Chemosphere"},{"key":"23789_CR53","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1016\/j.scitotenv.2019.01.245","volume":"662","author":"R Qiao","year":"2019","unstructured":"Qiao R, Sheng C, Lu Y, Zhang Y, Ren H, Lemos B (2019b) Microplastics induce intestinal inflammation, oxidative stress, and disorders of metabolome and microbiome in zebrafish. Sci Total Environ 662:246\u2013253","journal-title":"Sci Total Environ"},{"key":"23789_CR54","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.envres.2017.12.019","volume":"162","author":"S Rainieri","year":"2018","unstructured":"Rainieri S, Conlledo N, Larsen BK, Granby K, Barranco A (2018) Combined effects of microplastics and chemical contaminants on the organ toxicity of zebrafish (Danio rerio). Environ Res 162:135\u2013143","journal-title":"Environ Res"},{"key":"23789_CR55","doi-asserted-by":"crossref","unstructured":"Ratucoreh CY, Retnoaji B (2018) The growth and histology structure of Indonesian eel (Anguilla bicolor bicolor McClelland, 1844) fed with microalgae, AIP Conference Proceedings. AIP Publishing LLC, p. 020009","DOI":"10.1063\/1.5050105"},{"key":"23789_CR56","doi-asserted-by":"publisher","first-page":"3263","DOI":"10.1038\/srep03263","volume":"3","author":"CM Rochman","year":"2013","unstructured":"Rochman CM, Hoh E, Kurobe T, Teh SJ (2013) Ingested plastic transfers hazardous chemicals to fish and induces hepatic stress. Sci Rep 3:3263","journal-title":"Sci Rep"},{"key":"23789_CR57","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/j.aquaculture.2013.10.009","volume":"418","author":"K Sahin","year":"2014","unstructured":"Sahin K, Yazlak H, Orhan C, Tuzcu M, Akdemir F, Sahin N (2014) The effect of lycopene on antioxidant status in rainbow trout (Oncorhynchus mykiss) reared under high stocking density. Aquaculture 418:132\u2013138","journal-title":"Aquaculture"},{"key":"23789_CR58","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/j.ecoenv.2016.02.023","volume":"128","author":"AE-DH Sayed","year":"2016","unstructured":"Sayed AE-DH, Mohamed NH, Ismail MA, Abdel-Mageed WM, Shoreit AAM (2016) Antioxidant and antiapoptotic activities of Calotropis procera latex on Catfish (Clarias gariepinus) exposed to toxic 4-nonylphenol. Ecotoxicol Environ Saf 128:189\u2013194","journal-title":"Ecotoxicol Environ Saf"},{"key":"23789_CR59","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2021.112966","volume":"173","author":"AE-DH Sayed","year":"2021","unstructured":"Sayed AE-DH, Hamed M, Badrey AEA, Ismail RF, Osman YAA, Osman AGM, Soliman HAM (2021a) Microplastic distribution, abundance, and composition in the sediments, water, and fishes of the Red and Mediterranean seas, Egypt. Mar Pollut Bull 173:112966","journal-title":"Mar Pollut Bull"},{"key":"23789_CR60","volume":"250","author":"AH Sayed","year":"2021","unstructured":"Sayed AH, Hamed M, Badrey AEA, Soliman HAM (2021b) Bioremediation of hemotoxic and oxidative stress induced by polyethylene microplastic in Clarias gariepinus using lycopene, citric acid, and chlorella. Comp Biochem Physiol C: Toxicol Pharmacol 250:109189","journal-title":"Comp Biochem Physiol C: Toxicol Pharmacol"},{"key":"23789_CR61","doi-asserted-by":"publisher","first-page":"811466","DOI":"10.3389\/fenvs.2021.811466","volume":"9","author":"AEDH Sayed","year":"2022","unstructured":"Sayed AEDH, Hamed M, Ismail RF (2022) Natural antioxidants can improve microplastics-induced male reproductive impairment in the African catfish (Clarias gariepinus). Front Environ Sci 9:811466","journal-title":"Front Environ Sci"},{"key":"23789_CR62","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2020.114247","volume":"262","author":"E S\u0131kdokur","year":"2020","unstructured":"S\u0131kdokur E, Belivermi\u015f M, Sezer N, Pekmez M, Bulan \u00d6K, K\u0131l\u0131\u00e7 \u00d6 (2020) Effects of microplastics and mercury on manila clam Ruditapes philippinarum: feeding rate, immunomodulation, histopathology and oxidative stress. Environ Pollut 262:114247","journal-title":"Environ Pollut"},{"key":"23789_CR63","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1016\/j.envpol.2019.04.066","volume":"250","author":"Y Song","year":"2019","unstructured":"Song Y, Cao C, Qiu R, Hu J, Liu M, Lu S, Shi H, Raley-Susman KM, He D (2019) Uptake and adverse effects of polyethylene terephthalate microplastics fibers on terrestrial snails (Achatina fulica) after soil exposure. Environ Pollut 250:447\u2013455","journal-title":"Environ Pollut"},{"key":"23789_CR64","doi-asserted-by":"publisher","DOI":"10.1016\/j.cogsc.2021.100464","volume":"29","author":"I V\u00e1zquez-Rowe","year":"2021","unstructured":"V\u00e1zquez-Rowe I, Ita-Nagy D, Kahhat R (2021) Microplastics in fisheries and aquaculture: implications to food sustainability and safety. Curr Opin Green Sustain Chem 29:100464","journal-title":"Curr Opin Green Sustain Chem"},{"key":"23789_CR65","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1016\/j.jnutbio.2017.09.006","volume":"51","author":"J Xia","year":"2018","unstructured":"Xia J, Lin J, Li X-N, Zhang C, Li N, Du Z-H, Li Y-H, Li J-L (2018a) Atrazine-induced environmental nephrosis was mitigated by lycopene via modulating nuclear xenobiotic receptors-mediated response. J Nutr Biochem 51:80\u201390","journal-title":"J Nutr Biochem"},{"key":"23789_CR66","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1016\/j.envpol.2017.12.088","volume":"235","author":"J Xia","year":"2018","unstructured":"Xia J, Pan Z, Yang J, Wang W, Fu Z (2018) Polystyrene microplastics induce microbiota dysbiosis and inflammation in the gut of adult zebrafish. Environ Pollut (Barking, Essex\u202f: 1987) 235:322\u2013329","journal-title":"Environ Pollut (Barking, Essex : 1987)"},{"key":"23789_CR67","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.scitotenv.2019.136479","volume":"716","author":"X Xia","year":"2020","unstructured":"Xia X, Sun M, Zhou M, Chang Z, Li L (2020) Polyvinyl chloride microplastics induce growth inhibition and oxidative stress in Cyprinus carpio var. larvae. Sci Total Environ 716:7","journal-title":"Sci Total Environ"},{"key":"23789_CR68","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2020.122058","volume":"388","author":"H Yang","year":"2020","unstructured":"Yang H, Xiong H, Mi K, Xue W, Wei W, Zhang Y (2020) Toxicity comparison of nano-sized and micron-sized microplastics to Goldfish Carassius auratus Larvae. J Hazard Mater 388:122058","journal-title":"J Hazard Mater"},{"key":"23789_CR69","doi-asserted-by":"crossref","unstructured":"Yanuhar U, Arfiati D, Musa M, Junirahma NS, Caesar NR (2020) The status of VNN (viral nervous necrosis)-infected grouper fish tissue with chlorella vulgaris extract as anti-virus candidate, Journal of Physics: Conference Series. IOP Publishing, p. 012036","DOI":"10.1088\/1742-6596\/1665\/1\/012036"},{"key":"23789_CR70","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.jhazmat.2018.07.110","volume":"360","author":"L Yin","year":"2018","unstructured":"Yin L, Chen B, Xia B, Shi X, Qu K (2018) Polystyrene microplastics alter the behavior, energy reserve and nutritional composition of marine jacopever (Sebastes schlegelii). J Hazard Mater 360:97\u2013105","journal-title":"J Hazard Mater"},{"key":"23789_CR71","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.aquatox.2018.04.015","volume":"200","author":"P Yu","year":"2018","unstructured":"Yu P, Liu Z, Wu D, Chen M, Lv W, Zhao Y (2018) Accumulation of polystyrene microplastics in juvenile Eriocheir sinensis and oxidative stress effects in the liver. Aquat Toxicol 200:28\u201336","journal-title":"Aquat Toxicol"},{"key":"23789_CR72","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1007\/s12562-018-1274-6","volume":"85","author":"E Zahran","year":"2019","unstructured":"Zahran E, Awadin W, Risha E, Khaled AA, Wang T (2019) Dietary supplementation of Chlorella vulgaris ameliorates chronic sodium arsenite toxicity in Nile tilapia Oreochromis niloticus as revealed by histopathological, biochemical and immune gene expression analysis. Fish Sci 85:199\u2013215","journal-title":"Fish Sci"},{"key":"23789_CR73","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/j.aquaculture.2015.11.032","volume":"453","author":"H Zhang","year":"2016","unstructured":"Zhang H, Yi L, Sun R, Zhou H, Xu W, Zhang W, Mai K (2016) Effects of dietary citric acid on growth performance, mineral status and intestinal digestive enzyme activities of large yellow croaker Larimichthys crocea (Richardson, 1846) fed high plant protein diets. Aquaculture 453:147\u2013153","journal-title":"Aquaculture"},{"key":"23789_CR74","doi-asserted-by":"crossref","unstructured":"Zhang F, Li D, Yang Y, Zhang H, Zhu J, Liu J, ... Wang X (2022) Combined effects of polystyrene microplastics and copper on antioxidant capacity, immune response and intestinal microbiota of Nile tilapia (Oreochromis niloticus). Sci Total Environ 808: 152099","DOI":"10.1016\/j.scitotenv.2021.152099"},{"key":"23789_CR75","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2019.105396","volume":"220","author":"M Zhu","year":"2020","unstructured":"Zhu M, Chernick M, Rittschof D, Hinton DE (2020) Chronic dietary exposure to polystyrene microplastics in maturing Japanese medaka (Oryzias latipes). Aquat Toxicol 220:105396","journal-title":"Aquat Toxicol"},{"key":"23789_CR76","doi-asserted-by":"publisher","first-page":"152743","DOI":"10.1016\/j.scitotenv.2021.152743","volume":"815","author":"M Zazouli","year":"2022","unstructured":"Zazouli M, Nejati H, Hashempour Y, Dehbandi R, Fakhri Y (2022) Occurrence of microplastics (MPs) in the gastrointestinal tract of fishes: a global systematic review and meta-analysis and meta-regression. Sci Total Environ 815:152743","journal-title":"Sci Total Environ"},{"issue":"7","key":"23789_CR77","doi-asserted-by":"publisher","first-page":"2031","DOI":"10.1021\/acs.jafc.9b06882","volume":"68","author":"Y Zhao","year":"2019","unstructured":"Zhao Y, Li MZ, Shen Y, Lin J, Wang HR, Talukder M, Li JL (2019) Lycopene prevents DEHP-induced Leydig cell damage with the Nrf2 antioxidant signaling pathway in mice. J Agric Food Chem 68(7):2031\u20132040","journal-title":"J Agric Food Chem"},{"issue":"285","key":"23789_CR78","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2021.117080","volume":"15","author":"Y Zhao","year":"2021","unstructured":"Zhao Y, Bao RK, Zhu SY, Talukder M, Cui JG, Zhang H, Li XN, Li JL (2021) Lycopene prevents DEHP-induced hepatic oxidative stress damage by crosstalk between AHR-Nrf2 pathway. Environ Pollut 15(285):117080. https:\/\/doi.org\/10.1016\/j.envpol.2021.117080","journal-title":"Environ Pollut"},{"key":"23789_CR79","doi-asserted-by":"publisher","first-page":"8547","DOI":"10.1039\/D0FO01638A","volume":"11","author":"H Zhao","year":"2020","unstructured":"Zhao H, Wang Y, Mu M, Guo M, Yu H, Xing M (2020a) Lycopene alleviates sulfamethoxazole-induced hepatotoxicity in grass carp (Ctenopharyngodon idellus) via suppression of oxidative stress, inflammation and apoptosis. Food Funct 11:8547\u20138559","journal-title":"Food Funct"},{"issue":"41","key":"23789_CR80","doi-asserted-by":"publisher","first-page":"11468","DOI":"10.1021\/acs.jafc.0c05077","volume":"68","author":"Y Zhao","year":"2020","unstructured":"Zhao Y, Ma DX, Wang HG, Li MZ, Talukder M, Wang HR, Li JL (2020b) Lycopene prevents DEHP-induced liver lipid metabolism disorder by inhibiting the HIF-1\u03b1-induced PPAR\u03b1\/PPAR\u03b3\/FXR\/LXR system. J Agric Food Chem 68(41):11468\u201311479","journal-title":"J Agric Food Chem"},{"issue":"1","key":"23789_CR81","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1111\/raq.12496","volume":"13","author":"A Zhou","year":"2021","unstructured":"Zhou A, Zhang Y, Xie S, Chen Y, Li X, Wang J, Zou J (2021) Microplastics and their potential effects on the aquaculture systems: a critical review. Rev Aquac 13(1):719\u2013733","journal-title":"Rev Aquac"},{"key":"23789_CR82","volume":"261","author":"Z Zuo","year":"2022","unstructured":"Zuo Z, Wang Q, Zhang C, Zou J (2022) Single and combined effects of microplastics and cadmium on juvenile grass carp (Ctenopharyngodon idellus). Comp Biochem Physiol C: Toxicol Pharmacol 261:109424","journal-title":"Comp Biochem Physiol C: Toxicol Pharmacol"}],"container-title":["Environmental Science and Pollution Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11356-022-23789-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11356-022-23789-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11356-022-23789-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,19]],"date-time":"2023-02-19T05:37:23Z","timestamp":1676785043000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11356-022-23789-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,7]]},"references-count":82,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,2]]}},"alternative-id":["23789"],"URL":"https:\/\/doi.org\/10.1007\/s11356-022-23789-w","relation":{},"ISSN":["1614-7499"],"issn-type":[{"value":"1614-7499","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,11,7]]},"assertion":[{"value":"24 May 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 October 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 November 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The experimental setup and fish handling procedure were approved by the Research Ethical Committee of the Faculty of Science, Assiut University, Assiut, Egypt.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}