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Anaerobic biodegradation is crucial for its removal from contaminated environments, yet prevailing mechanisms remain unresolved, especially concerning dehalogenation. In this study, we obtained an assembled genome of a novel DCM-degrading strain,\n                    <jats:italic>Dehalobacterium formicoaceticum<\/jats:italic>\n                    strain EZ94, from a stable DCM-degrading consortium, and we analyzed its proteome during degradation of DCM. A gene cluster recently predicted to play a major role in anaerobic DCM catabolism (the\n                    <jats:italic>mec<\/jats:italic>\n                    cassette) was found. Methyltransferases and other proteins encoded by the\n                    <jats:italic>mec<\/jats:italic>\n                    cassette were among the most abundant proteins produced, suggesting their involvement in DCM catabolism. Reductive dehalogenases were not detected. Genes and corresponding proteins for a complete Wood-Ljungdahl pathway, which could enable further metabolism of DCM carbon, were also found. Unlike for the anaerobic DCM degrader \u201c\n                    <jats:italic>Ca<\/jats:italic>\n                    . F. warabiya,\u201d no genes for metabolism of the quaternary amines choline and glycine betaine were identified. This work provides independent and supporting evidence that\n                    <jats:italic>mec<\/jats:italic>\n                    -associated methyltransferases are key to anaerobic DCM metabolism.\n                  <\/jats:p>","DOI":"10.1007\/s11356-023-28144-1","type":"journal-article","created":{"date-parts":[[2023,6,10]],"date-time":"2023-06-10T01:01:37Z","timestamp":1686358897000},"page":"80602-80612","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Proteogenomics of the novel Dehalobacterium formicoaceticum strain EZ94 highlights a key role of methyltransferases during anaerobic dichloromethane degradation"],"prefix":"10.1007","volume":"30","author":[{"given":"Kenneth","family":"Wasmund","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alba","family":"Trueba-Santiso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Vicent","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenz","family":"Adrian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Vuilleumier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8033-6553","authenticated-orcid":false,"given":"Ernest","family":"Marco-Urrea","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,6,10]]},"reference":[{"key":"28144_CR1","unstructured":"Andrews S (2010) FastQC: a quality control tool for high throughput sequence data. http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc. 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