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Species identified were <jats:italic>Staphylococcus aureus<\/jats:italic>, <jats:italic>S. lentus<\/jats:italic>, <jats:italic>S. arlettae<\/jats:italic>, <jats:italic>S. cohnii<\/jats:italic>, <jats:italic>S. haemolyticus<\/jats:italic>, <jats:italic>S. nepalensis<\/jats:italic>, <jats:italic>S. sciuri\u00a0<\/jats:italic>(now <jats:italic>Mammaliicoccus sciuri<\/jats:italic>), and <jats:italic>S. xylosus<\/jats:italic>. Isolates showed high resistance to methicillin (91%), ampicillin (89%), ciprofloxacin (86%), amoxycillin (80%), ceftazidime (74%), and cloxacillin (71%). Multiple antibiotic resistance (MAR) index for the isolates exceeded 0.2 (0.50\u20130.70). Among the isolates, 77% were <jats:italic>mecA<\/jats:italic>-positive. All <jats:italic>S. aureus<\/jats:italic> strains were positive for <jats:italic>nuc<\/jats:italic> and 7 <jats:italic>Spa<\/jats:italic> gene types. The present study highlights possibility of treated wastewaters being potential reservoir for antibiotic-resistant staphylococci. This is a cause for concern as wastewater effluents are decanted into environmental waters and these are, in many cases, used for various purposes including recreation (full contact), religious (full body submersion), and drinking water for some rural communities and water for livestock.<\/jats:p>","DOI":"10.1007\/s11356-023-30319-9","type":"journal-article","created":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T08:02:27Z","timestamp":1697875347000},"page":"117165-117178","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Detection of mecA positive staphylococcal species in a wastewater treatment plant in South Africa"],"prefix":"10.1007","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9793-1722","authenticated-orcid":false,"given":"Adegboyega Oyedele","family":"Oladipo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluwatosin Gbemisola","family":"Oladipo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos Cornelius","family":"Bezuidenhout","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,10,21]]},"reference":[{"key":"30319_CR1","doi-asserted-by":"publisher","first-page":"100541","DOI":"10.1016\/j.genrep.2019.100541","volume":"17","author":"AO Adekanmbi","year":"2019","unstructured":"Adekanmbi AO, Soyoye OF, Adelowo OO (2019) Characterization of methicillin-resistance gene mecA in coagulase negative staphylococci (CoNS) recovered from wastewater of two healthcare facilities in Nigeria. 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