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Therefore, it is important to understand their impact on the environment. Aquatic ecosystems are subject to EMF as part of various electricity sources, e.g., high-voltage transmission lines (HVTL). We examined the impact of EMF generated by HVTL on the spatial arrangement and survival of pike (<jats:italic>Esox lucius<\/jats:italic>) embryos. Fertilized eggs were incubated under two HVTL configurations 110\u00a0kV and 220\u00a0kV compared with a control group devoid of anthropogenic EMF. Embryo orientation and survival were monitored until blastopore closure. The control group showed dominance in the arrangement of embryos along the N-S, NNW-SSE, and NNE-SSW axes, with a slight prevalence of northern directions. EMF originating from HVTL did not exert a significant influence on the spatial arrangement of pike embryos, although some deviations from the arrangement noticed in the control group were observed. Increased embryo mortality was observed only at 110\u00a0kV site, but probably due to factors unrelated to EMF. In conclusion, EMF generated by HVTL did not significantly change pike embryo orientation or chances of survival. However, longer exposure or higher EMF levels could provoke notable reactions, requiring ongoing evaluation as power networks continue to spread more widely.<\/jats:p>","DOI":"10.1007\/s11356-024-34300-y","type":"journal-article","created":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T02:01:37Z","timestamp":1720749697000},"page":"47291-47297","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The impact of electromagnetic fields generated by high-voltage power lines on the spatial arrangement of pike (Esox Lucius Linnaeus 1758) embryos"],"prefix":"10.1007","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8415-8443","authenticated-orcid":false,"given":"Jan","family":"Krzystolik","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3537-4198","authenticated-orcid":false,"given":"Adam","family":"Ta\u0144ski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6948-4240","authenticated-orcid":false,"given":"Rados\u0142aw","family":"Piesiewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0068-4011","authenticated-orcid":false,"given":"Krzysztof","family":"Formicki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,7,12]]},"reference":[{"key":"34300_CR1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s10661-016-5318-z","volume":"188","author":"E Al-Bassam","year":"2016","unstructured":"Al-Bassam E, Elumalai A, Khan A, Al-Awadi L (2016) Assessment of electromagnetic field levels from surrounding high-tension overhead power lines for proposed land use. 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