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Subsequently, water\/wastewater systems have become established as reservoirs of antimicrobial resistance determinants in the environment. In this study, we carried out the synthesis and characterization of magnetite nanoparticles for use in the removal of genomic DNA of antibiotic resistant\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    isolate in aqueous system. The synthesis of this nanoparticle was achieved by using precipitation method and characterization of the material was conducted by using Fourier transform infrared spectroscopy (FTIR), scanning electron spectroscopy (SEM), electron diffraction spectroscopy (EDS), and vibrating sample magnetometry (VSM). The SEM analysis revealed that this material is spherical in shape, while the FTIR spectra revealed Fe\u2013O vibrational band at\u2009~\u2009450\u00a0cm\n                    <jats:sup>\u22121<\/jats:sup>\n                    confirming the success of the synthesis of this material. The magnetite nanomaterial showed efficiency for the removal of the bacterial DNA from water with a maximum removal capacity of 45.5\u00a0ng\u00a0g\n                    <jats:sup>\u22121<\/jats:sup>\n                    . The effect of pH (\n                    <jats:italic>q<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>e<\/jats:italic>\n                    <\/jats:sub>\n                    max\u2009=\u200955\u00a0ng\u00a0g\n                    <jats:sup>\u22121<\/jats:sup>\n                    @ pH\u2009=\u200910), time (\n                    <jats:italic>q<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>t<\/jats:italic>\n                    <\/jats:sub>\n                    max @ 180\u00a0min), DNA concentration (DNA concentration of 185\u00a0ng\/mL,\n                    <jats:italic>q<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>e<\/jats:italic>\n                    <\/jats:sub>\n                    max\u2009=\u200945.5\u00a0ng\u00a0g\n                    <jats:sup>\u22121<\/jats:sup>\n                    ), and adsorbent weight (% adsorption max\u2009=\u200961.65% @ 0.025\u00a0g) suggest that adsorption conditions influence the removal of DNA by this material. In addition, kinetic study shows that the removal of bacterial DNA obeyed pseudo 2nd order indicating that adsorption process was achieved by bimolecular interaction between magnetite and antibiotics resistant bacterial DNA. We conclude that magnetite nanoparticle appears to be a potential candidate for the removal of nucleic acids and antimicrobial resistance determinants in water\/wastewater treatment.\n                  <\/jats:p>","DOI":"10.1007\/s11356-024-35098-5","type":"journal-article","created":{"date-parts":[[2024,9,29]],"date-time":"2024-09-29T21:01:32Z","timestamp":1727643692000},"page":"30135-30145","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effectiveness of magnetite nanoparticles for the removal of DNA of multidrug-resistant Escherichia coli from municipal wastewater"],"prefix":"10.1007","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4038-7639","authenticated-orcid":false,"given":"Mike O.","family":"Ojemaye","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omobola O.","family":"Okoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony I.","family":"Okoh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2024,9,30]]},"reference":[{"key":"35098_CR1","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1002\/mbo3.319","volume":"5","author":"MA Adefisoye","year":"2016","unstructured":"Adefisoye MA, Okoh AI (2016) Identification and antimicrobial resistance prevalence of pathogenic Escherichia coli strains from treated wastewater effluents in Eastern Cape, South Africa. 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Therefore, ethical approval was not required.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval"}},{"value":"All authors agree to participate in this study.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to participate"}},{"value":"All authors agree to the publication of this manuscript.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for publication"}},{"value":"The authors declare no competing interests.","order":6,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}