{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:40:32Z","timestamp":1792442432620,"version":"4.5.0"},"reference-count":80,"publisher":"Springer Science and Business Media LLC","issue":"34","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Environ Sci Pollut Res"],"DOI":"10.1007\/s11356-026-38265-y","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:03:59Z","timestamp":1791183839000},"page":"16828-16842","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Multi-biomarker evaluation of the effects of a glyphosate-based commercial formulation and its active ingredient on the green microalga Scenedesmus vacuolatus"],"prefix":"10.1007","volume":"33","author":[{"given":"Mar\u00eda Mercedes","family":"Iummato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mart\u00edn","family":"Graziano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda del Carmen","family":"R\u00edos de Molina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1ngela Beatriz","family":"Ju\u00e1rez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"key":"38265_CR1","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1016\/s0076-6879(84)05016-3","volume":"105","author":"H Aebi","year":"1984","unstructured":"Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121\u2013126. https:\/\/doi.org\/10.1016\/s0076-6879(84)05016-3","journal-title":"Methods Enzymol"},{"key":"38265_CR2","doi-asserted-by":"publisher","first-page":"1062","DOI":"10.1016\/j.plaphy.2008.07.002","volume":"46","author":"N Ahsan","year":"2008","unstructured":"Ahsan N, Lee DG, Lee KW, Alam I, Lee SH, Bahk JD, Lee BH (2008) Glyphosate-induced oxidative stress in rice leaves revealed by proteomic approach. Plant Physiol Biochem 46:1062\u20131070. https:\/\/doi.org\/10.1016\/j.plaphy.2008.07.002","journal-title":"Plant Physiol Biochem"},{"key":"38265_CR3","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1016\/j.pestbp.2004.11.009","volume":"82","author":"CJ Andrews","year":"2005","unstructured":"Andrews CJ, Cummins I, Skipsey M, Grundy NM, Jepson I, Townson J, Edwards R (2005) Purification and characterization of a family of glutathione transferases with roles in herbicide detoxification in soybean (Glycine max L.); selective enhancement by herbicides and herbicide safeners. Pestic Biochem Physiol 82:205\u2013219. https:\/\/doi.org\/10.1016\/j.pestbp.2004.11.009","journal-title":"Pestic Biochem Physiol"},{"key":"38265_CR4","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1002\/jat.2997","volume":"34","author":"R Annett","year":"2014","unstructured":"Annett R, Habibi HR, Hontela A (2014) Impact of glyphosate and glyphosate-based herbicides on the freshwater environment. J Appl Toxicol 34:458\u2013479. https:\/\/doi.org\/10.1002\/jat.2997","journal-title":"J Appl Toxicol"},{"key":"38265_CR5","doi-asserted-by":"publisher","first-page":"334","DOI":"10.1016\/j.ecoenv.2015.06.015","volume":"120","author":"V Asselborn","year":"2015","unstructured":"Asselborn V, Fern\u00e1ndez C, Zalocar Y, Parodi ER (2015) Effects of chlorpyrifos on the growth and ultrastructure of green algae, Ankistrodesmus gracilis. Ecotoxicol Environ Saf 120:334\u2013341. https:\/\/doi.org\/10.1016\/j.ecoenv.2015.06.015","journal-title":"Ecotoxicol Environ Saf"},{"key":"38265_CR6","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1016\/j.microc.2015.05.004","volume":"122","author":"E Avigliano","year":"2015","unstructured":"Avigliano E, Schenone NF (2015) Human health risk assessment and environmental distribution of trace elements, glyphosate, fecal coliform and total coliform in Atlantic Rainforest mountain rivers (South America). Microchem J 122:149\u2013158. https:\/\/doi.org\/10.1016\/j.microc.2015.05.004","journal-title":"Microchem J"},{"key":"38265_CR7","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.envexpbot.2013.12.026","volume":"101","author":"B Basa","year":"2014","unstructured":"Basa B, Lattanzio G, Solti A, T\u00f3th B, Abad\u00eda J, Fodor F, S\u00e1rv\u00e1ri \u00c9 (2014) Changes induced by cadmium stress and iron deficiency in the composition and organization of thylakoid complexes in sugar beet (Beta vulgaris L.). Environ Exp Bot 101:1\u201311. https:\/\/doi.org\/10.1016\/j.envexpbot.2013.12.026","journal-title":"Environ Exp Bot"},{"key":"38265_CR8","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.pestbp.2010.11.007","volume":"99","author":"M Basantani","year":"2011","unstructured":"Basantani M, Srivastava A, Sen S (2011) Elevated antioxidant response and induction of tau-class glutathione S-transferase after glyphosate treatment in Vigna radiata (L.) Wilczek. Pestic Biochem Physiol 99:111\u2013117. https:\/\/doi.org\/10.1016\/j.pestbp.2010.11.007","journal-title":"Pestic Biochem Physiol"},{"key":"38265_CR9","doi-asserted-by":"publisher","unstructured":"Bas\u00edlico G, Cogollo-Rueda M, Ionno V, Faggi A, de Cabo L (2022) The use of glyphosate in regions of Argentina and Colombia and its socio-environmental impacts. In: Naeem M, Bremont JFJ, Ansari AA, Gill SS (eds) Agrochemicals in soil and environment. Springer, Singapore, pp 195\u2013212. https:\/\/doi.org\/10.1007\/978-981-16-9310-6_9","DOI":"10.1007\/978-981-16-9310-6_9"},{"key":"38265_CR10","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1016\/0003-2697(71)90370-8","volume":"44","author":"C Beauchamp","year":"1971","unstructured":"Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276\u2013286. https:\/\/doi.org\/10.1016\/0003-2697(71)90370-8","journal-title":"Anal Biochem"},{"key":"38265_CR11","doi-asserted-by":"publisher","unstructured":"Bialk-Bielinska A, Caban M, Pieczynska A, Stepnowski P, Stolte S (2017) Mixture toxicity of six sulfonamides and their two transformation products to green algae Scenedesmus vacuolatus and duckweed Lemna minor. Chemosphere 173:542\u2013550. https:\/\/doi.org\/10.1016\/j.chemosphere.2017.01.035","DOI":"10.1016\/j.chemosphere.2017.01.035"},{"key":"38265_CR12","first-page":"1","volume":"6318","author":"HW Bischoff","year":"1963","unstructured":"Bischoff HW, Bold HC (1963) Phycological studies IV. Some soil algae from Enchanted Rock and related algal species. Univ Tex Publ 6318:1\u201395","journal-title":"Univ Tex Publ"},{"key":"38265_CR13","doi-asserted-by":"publisher","first-page":"2585","DOI":"10.1093\/jxb\/ert466","volume":"65","author":"K Bi\u0161ov\u00e1","year":"2014","unstructured":"Bi\u0161ov\u00e1 K, Zachleder V (2014) Cell-cycle regulation in green algae dividing by multiple fission. J Exp Bot 65:2585\u20132602. https:\/\/doi.org\/10.1093\/jxb\/ert466","journal-title":"J Exp Bot"},{"key":"38265_CR14","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","author":"MM Bradford","year":"1976","unstructured":"Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities utilizing the principle of protein dye binding. Anal Biochem 72:248\u2013254. https:\/\/doi.org\/10.1016\/0003-2697(76)90527-3","journal-title":"Anal Biochem"},{"key":"38265_CR15","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1042\/bj1390043","volume":"139","author":"RC Bray","year":"1974","unstructured":"Bray RC, Cockle SA, Fielden EM, Roberts PB, Rotilio G, Calabrese L (1974) Reduction and inactivation of superoxide dismutase by hydrogen peroxide. Biochem J 139:43\u201348. https:\/\/doi.org\/10.1042\/bj1390043","journal-title":"Biochem J"},{"key":"38265_CR16","doi-asserted-by":"publisher","first-page":"60635","DOI":"10.1007\/s11356-021-14609-8","volume":"28","author":"EM Brovini","year":"2021","unstructured":"Brovini EM, Cardoso- SJ, Quadra GR, Vilas-Boas JA, Paranaiba JR, Oliveira Pereira R, Fernandes Mendonca R (2021) Glyphosate concentrations in global freshwaters: are aquatic organisms at risk? Environ Sci Pollut Res 28:60635\u201360648. https:\/\/doi.org\/10.1007\/s11356-021-14609-8","journal-title":"Environ Sci Pollut Res"},{"key":"38265_CR17","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/S0043-1354(00)00223-2","volume":"35","author":"L Campanella","year":"2000","unstructured":"Campanella L, Cubadda F, Sammartino MP, Saoncella A (2000) An algal biosensor for the monitoring of water toxicity in estuarine environments. Water Res 35:69\u201376. https:\/\/doi.org\/10.1016\/S0043-1354(00)00223-2","journal-title":"Water Res"},{"key":"38265_CR18","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1016\/j.plipres.2013.07.001","volume":"52","author":"I Domonkos","year":"2013","unstructured":"Domonkos I, Kis M, Gombos Z, Ughy B (2013) Carotenoids, versatile components of oxygenic photosynthesis. Prog Lipid Res 52:539\u2013561. https:\/\/doi.org\/10.1016\/j.plipres.2013.07.001","journal-title":"Prog Lipid Res"},{"key":"38265_CR19","doi-asserted-by":"publisher","first-page":"51860","DOI":"10.1007\/s11356-022-19517-z","volume":"29","author":"Y Fan","year":"2022","unstructured":"Fan Y, Zhang K, Zhang R, Guo G, Li H, Bai Y, Lin Y, Cai T (2022) Derivation of water quality criteria for glyphosate and its formulations to protect aquatic life in China. Environ Sci Pollut Res 29:51860\u201351870. https:\/\/doi.org\/10.1007\/s11356-022-19517-z","journal-title":"Environ Sci Pollut Res"},{"key":"38265_CR20","doi-asserted-by":"publisher","first-page":"31384","DOI":"10.1007\/s11356-021-17962-w","volume":"29","author":"AV Fassiano","year":"2022","unstructured":"Fassiano AV, March H, Santos M, Ju\u00e1rez AB, R\u00edos de Molina MC (2022) Toxicological effects of active and inert ingredients of imazethapyr formulation Verosil\u00ae against Scenedesmus vacuolatus (Chlorophyta). Environ Sci Pollut Res 29:31384\u201331399. https:\/\/doi.org\/10.1007\/s11356-021-17962-w","journal-title":"Environ Sci Pollut Res"},{"key":"38265_CR21","doi-asserted-by":"publisher","DOI":"10.1016\/j.jece.2024.114072","volume":"12","author":"I Fernandes Farah","year":"2024","unstructured":"Fernandes Farah I, Rodrigues dos Santos C, Ferreira Pinto MC, Righi Ara\u00fajo C, Santos Amaral MC (2024) Pesticides in aquatic environment: occurrence, ecological implications and legal framework. J Environ Chem Eng 12:114072. https:\/\/doi.org\/10.1016\/j.jece.2024.114072","journal-title":"J Environ Chem Eng"},{"key":"38265_CR22","doi-asserted-by":"publisher","first-page":"18869","DOI":"10.1007\/s11356-016-7005-6","volume":"23","author":"F Gatt\u00e1s","year":"2016","unstructured":"Gatt\u00e1s F, Vinocur A, Graziano M, dos Santos Afonso M, Pizarro H, Cataldo D (2016) Differential impact of Limnoperna fortunei-herbicide interaction between Roundup Max\u00ae and glyphosate on freshwater microscopic communities. Environ Sci Pollut Res Int 23:18869\u201318882. https:\/\/doi.org\/10.1007\/s11356-016-7005-6","journal-title":"Environ Sci Pollut Res Int"},{"key":"38265_CR23","doi-asserted-by":"publisher","unstructured":"Gomes MP, Gingras Le Manac\u00b4h S, Maccario S, Labrecque M, Lucotte M, Juneau P (2016) Differential effects of glyphosate and aminomethylphosphonic acid (AMPA) on photosynthesis and chlorophyll metabolism in willow plants. Pestic Biochem Phys 130:65\u201370. https:\/\/doi.org\/10.1016\/j.pestbp.2015.11.010","DOI":"10.1016\/j.pestbp.2015.11.010"},{"key":"38265_CR24","doi-asserted-by":"publisher","unstructured":"Gon\u00e7alves AMM, Rocha CP, Marques JC, Gon\u00e7alves FJM (2021) Enzymes as useful biomarkers to assess the response of freshwater communities to pesticide exposure: a review. Ecol Indic 122:107303. https:\/\/doi.org\/10.1016\/j.ecolind.2020.107303","DOI":"10.1016\/j.ecolind.2020.107303"},{"key":"38265_CR25","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1016\/j.biortech.2013.10.073","volume":"151","author":"BS Grama","year":"2014","unstructured":"Grama BS, Chader S, Khelifi D, Agathos SN, Jeffryes C (2014) Induction of canthaxanthin production in a Dactylococcus microalga isolated from the Algerian Sahara. Bioresour Technol 151:297\u2013305. https:\/\/doi.org\/10.1016\/j.biortech.2013.10.073","journal-title":"Bioresour Technol"},{"key":"38265_CR26","doi-asserted-by":"publisher","first-page":"7130","DOI":"10.1016\/S0021-9258(19)42083-8","volume":"249","author":"W Habig","year":"1974","unstructured":"Habig W, Pabst M, Jakoby W (1974) Glutathione S-transferases: the first enzymatic step in mercapturic acid formation. J Biol Chem 249:7130\u20137139","journal-title":"J Biol Chem"},{"key":"38265_CR27","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2020.137186","volume":"717","author":"CI Hern\u00e1ndez-Garc\u00eda","year":"2020","unstructured":"Hern\u00e1ndez-Garc\u00eda CI, Mart\u00ednez-Jer\u00f3nimo F (2020) Multistressor negative effects on an experimental phytoplankton community: the case of glyphosate and one toxigenic cyanobacterium on Chlorophycean microalgae. Sci Total Environ 717:137186. https:\/\/doi.org\/10.1016\/j.scitotenv.2020.137186","journal-title":"Sci Total Environ"},{"key":"38265_CR28","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1046\/j.1529-8817.1999.3520403.x","volume":"35","author":"H Hillebrand","year":"1999","unstructured":"Hillebrand H, Durselen C, Kirschtel D, Pollingher U (1999) Biovolume calculation for pelagic and benthic microalgae. J Phycol 35:403\u2013424. https:\/\/doi.org\/10.1046\/j.1529-8817.1999.3520403.x","journal-title":"J Phycol"},{"key":"38265_CR29","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1007\/s004250050524","volume":"207","author":"DM Hodges","year":"1999","unstructured":"Hodges DM, Delong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid- reactive substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and others interfering compounds. Planta 207:604\u2013611. https:\/\/doi.org\/10.1007\/s004250050524","journal-title":"Planta"},{"key":"38265_CR30","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1016\/j.ecoenv.2019.01.083","volume":"172","author":"MM Iummato","year":"2019","unstructured":"Iummato MM, Fassiano AB, Graziano M, dos Santos M, R\u00edos de Molina MC, Ju\u00e1rez AB (2019) Effect of glyphosate on the growth, morphology, ultrastructure and metabolism of Scenedesmus vacuolatus. Ecotoxicol Environ Saf 172:471\u2013479. https:\/\/doi.org\/10.1016\/j.ecoenv.2019.01.083","journal-title":"Ecotoxicol Environ Saf"},{"key":"38265_CR31","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/s0300-483x(01)00463-2","volume":"166","author":"M Jokanovi\u0107","year":"2001","unstructured":"Jokanovi\u0107 M (2001) Biotransformation of organophosphorus compounds. Toxicology 166:139\u2013160. https:\/\/doi.org\/10.1016\/s0300-483x(01)00463-2","journal-title":"Toxicology"},{"key":"38265_CR32","doi-asserted-by":"publisher","first-page":"620","DOI":"10.1080\/01926230290166724","volume":"30","author":"R Kohen","year":"2002","unstructured":"Kohen R, Nyska A (2002) Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol 30:620\u2013650. https:\/\/doi.org\/10.1080\/01926230290166724","journal-title":"Toxicol Pathol"},{"key":"38265_CR33","doi-asserted-by":"publisher","first-page":"5751","DOI":"10.1016\/S0021-9258(19)83842-5","volume":"257","author":"Y Kono","year":"1982","unstructured":"Kono Y, Fridovich I (1982) Superoxide radical inhibits catalase. J Biol Chem 257:5751\u20135754","journal-title":"J Biol Chem"},{"key":"38265_CR34","doi-asserted-by":"publisher","DOI":"10.1186\/1999-3110-54-3","volume":"54","author":"TC Lee","year":"2013","unstructured":"Lee TC, Hsu BD (2013) Characterization of the decline and recovery of heat-treated Scenedesmus vacuolatus. Bot Stud 54:3. https:\/\/doi.org\/10.1186\/1999-3110-54-3","journal-title":"Bot Stud"},{"key":"38265_CR35","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/0076-6879(87)48036-1","volume":"148","author":"HK Lichtenthaler","year":"1987","unstructured":"Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Methods Enzymol 148:349\u2013382. https:\/\/doi.org\/10.1016\/0076-6879(87)48036-1","journal-title":"Methods Enzymol"},{"key":"38265_CR36","doi-asserted-by":"publisher","first-page":"20913","DOI":"10.3390\/ijms141020913","volume":"14","author":"YH Lin","year":"2013","unstructured":"Lin YH, Pan K, Hung CH, Huang HE, Chen CL, Feng TY, Huang LF (2013) Overexpression of ferredoxin, PETF, enhances tolerance to heat stress in Chlamydomonas reinhardtii. Int J Mol Sci 14:20913\u201320929. https:\/\/doi.org\/10.3390\/ijms141020913","journal-title":"Int J Mol Sci"},{"key":"38265_CR37","doi-asserted-by":"publisher","first-page":"1681","DOI":"10.1016\/j.ecoenv.2010.08.017","volume":"73","author":"J Lipok","year":"2010","unstructured":"Lipok J, Studnik H, Gruyaert S (2010) The toxicity of Roundup\u00ae 360 SL formulation and its main constituents: glyphosate and isopropylamine towards non-target water photoautotrophs. Ecotoxicol Environ Saf 73:1681\u20131688. https:\/\/doi.org\/10.1016\/j.ecoenv.2010.08.017","journal-title":"Ecotoxicol Environ Saf"},{"key":"38265_CR38","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.aquatox.2009.04.006","volume":"93","author":"H Liu","year":"2009","unstructured":"Liu H, Xiong M (2009) Comparative toxicity of racemic metolachlor and S-metolachlor to Chlorella pyrenoidosa. Aquat Toxicol 93:100\u2013106. https:\/\/doi.org\/10.1016\/j.aquatox.2009.04.006","journal-title":"Aquat Toxicol"},{"key":"38265_CR39","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2019.134557","volume":"711","author":"VF Lutri","year":"2020","unstructured":"Lutri VF, Matteoda E, Blarasin M, Aparicio V, Giacobone D, Maldonado L, Becher Quinodoz F, Cabrera A, Giuliano Albo J (2020) Hydrogeological features affecting spatial distribution of glyphosate and AMPA in groundwater and surface water in an agroecosystem. C\u00f3rdoba, Argentina. Sci Total Environ 711:134557. https:\/\/doi.org\/10.1016\/j.scitotenv.2019.134557","journal-title":"Sci Total Environ"},{"key":"38265_CR40","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2020.105449","volume":"222","author":"MD Machado","year":"2020","unstructured":"Machado MD, Soares EV (2020) Reproductive cycle progression arrest and modification of cell morphology (shape and biovolume) in the alga Pseudokirchneriella subcapitata exposed to metolachlor. Aquat Toxicol 222:105449. https:\/\/doi.org\/10.1016\/j.aquatox.2020.105449","journal-title":"Aquat Toxicol"},{"key":"38265_CR41","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquatox.2024.106851","volume":"268","author":"M Narayanan","year":"2024","unstructured":"Narayanan M, Devarayan K, Verma M, Selvaraj M, Ghramh HA, Kandasamy S (2024) Assessing the ecological impact of pesticides\/herbicides on algal communities: a comprehensive review. Aquat Toxicol 268:106851. https:\/\/doi.org\/10.1016\/j.aquatox.2024.106851","journal-title":"Aquat Toxicol"},{"key":"38265_CR42","unstructured":"OECD (2011) OECD guidelines for the testing of chemicals freshwater alga and cyanobacteria, Growth Inhibition Test. OECD 201, 22 pp."},{"key":"38265_CR43","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1007\/s002440010144","volume":"40","author":"OK Okamoto","year":"2001","unstructured":"Okamoto OK, Pinto E, Latorre LR, Bechara EJH, Colepicolo P (2001) Antioxidant modulation in response to metal-induced oxidative stress in algal chloroplasts. Arch Environ Contam Toxicol 40:18\u201324. https:\/\/doi.org\/10.1007\/s002440010144","journal-title":"Arch Environ Contam Toxicol"},{"key":"38265_CR44","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2020.125955","volume":"248","author":"JM Ostera","year":"2022","unstructured":"Ostera JM, Malanga G, Puntarulo S (2022) Assessment of oxidative balance in hydrophilic cellular environment in Chlorella vulgaris exposed to glyphosate. Chemosphere 248:125955. https:\/\/doi.org\/10.1016\/j.chemosphere.2020.125955","journal-title":"Chemosphere"},{"key":"38265_CR45","doi-asserted-by":"publisher","first-page":"1115","DOI":"10.1016\/j.chemosphere.2005.03.044","volume":"61","author":"F Peixoto","year":"2005","unstructured":"Peixoto F (2005) Comparative effects of the Roundup and glyphosate on mitochondrial oxidative phosphorylation. Chemosphere 61:1115\u20131122. https:\/\/doi.org\/10.1016\/j.chemosphere.2005.03.044","journal-title":"Chemosphere"},{"key":"38265_CR46","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1007\/s10646-009-0300-y","volume":"18","author":"JL Pereira","year":"2009","unstructured":"Pereira JL, Antunes SC, Castro BB, Masques CR, Gon\u00e7alves AMM, Gon\u00e7alves F, Pereira R (2009) Toxicity evaluation of three pesticides on non-target aquatic and soil organisms: commercial formulation versus active ingredient. Ecotoxicology 18:455\u2013463. https:\/\/doi.org\/10.1007\/s10646-009-0300-y","journal-title":"Ecotoxicology"},{"key":"38265_CR47","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.cotox.2018.12.006","volume":"13","author":"KS Pikula","year":"2019","unstructured":"Pikula KS, Zakharenko AM, Aruoja V, Golokhvast KS, Tsatsakis AM (2019) Oxidative stress and its biomarkers in microalgal ecotoxicology. Curr Opin Toxicol 13:8\u201315. https:\/\/doi.org\/10.1016\/j.cotox.2018.12.006","journal-title":"Curr Opin Toxicol"},{"key":"38265_CR48","doi-asserted-by":"publisher","first-page":"117","DOI":"10.1007\/s10811-011-9657-4","volume":"24","author":"P Pirastru","year":"2012","unstructured":"Pirastru P, Darwish M, Chu FL, Perreault F, Sirois L, Sleno L, Popovic R (2012) Carotenoid production and change of photosynthetic functions in Scenedesmus sp. exposed to nitrogen limitation and acetate treatment. J Appl Phycol 24:117\u2013124. https:\/\/doi.org\/10.1007\/s10811-011-9657-4","journal-title":"J Appl Phycol"},{"key":"38265_CR49","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1016\/s0076-6879(94)33041-7","volume":"233","author":"A Reznick","year":"1994","unstructured":"Reznick A, Packer L (1994) Oxidative damage to proteins: spectrophotometric method for carbonyl assay. Methods Enzymol 233:357\u2013363. https:\/\/doi.org\/10.1016\/s0076-6879(94)33041-7","journal-title":"Methods Enzymol"},{"key":"38265_CR50","doi-asserted-by":"publisher","first-page":"1517","DOI":"10.1128\/ec.00106-06","volume":"5","author":"TA Richards","year":"2006","unstructured":"Richards TA, Dacks JB, Campbell SA, Blanchard JL, Foster PG, McLeod R, Roberts CW (2006) Evolutionary origins of the eukaryotic shikimate pathway: gene fusions, horizontal gene transfer, and endosymbiotic replacements. Eukaryot Cell 5:1517\u20131531. https:\/\/doi.org\/10.1128\/ec.00106-06","journal-title":"Eukaryot Cell"},{"key":"38265_CR51","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/S0166-445X(01)00255-7","volume":"59","author":"C Rioboo","year":"2002","unstructured":"Rioboo C, Gonz\u00e1lez O, Herrero C, Cid A (2002) Physiological response of freshwater microalga (Chlorella vulgaris) to triazine and phenylurea herbicides. Aquat Toxicol 59:225\u2013235. https:\/\/doi.org\/10.1016\/S0166-445X(01)00255-7","journal-title":"Aquat Toxicol"},{"key":"38265_CR52","first-page":"129","volume":"255","author":"JL Rodr\u00edguez-Gil","year":"2021","unstructured":"Rodr\u00edguez-Gil JL, Prosser RS, Duke SO, Solomon KR (2021) Ecotoxicology of glyphosate, its formulants, and environmental degradation products. Rev Environ Contam Toxicol 255:129\u2013205.\u00a0https:\/\/doi.org\/10.1007\/398_2020_56","journal-title":"Rev Environ Contam Toxicol"},{"key":"38265_CR53","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1016\/j.ecoenv.2010.10.034","volume":"74","author":"D Romero","year":"2011","unstructured":"Romero D, R\u00edos de Molina MC, Ju\u00e1rez AB (2011) Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri. Ecotoxicol Environ Saf 74:741\u2013747. https:\/\/doi.org\/10.1016\/j.ecoenv.2010.10.034","journal-title":"Ecotoxicol Environ Saf"},{"key":"38265_CR54","unstructured":"Ronco A, Carriquiriborde P, Natale GS, Martin M, Mugni H, Bonetto C (2008) Integrated approach for the assessment of biotech soybean pesticides impact on low order stream ecosystems of the Pampasic region. In: Chen J, Guo C (eds) Ecosystem ecology research trends. Nova Science Publisher Inc, New York, USA, p pp 209-239"},{"key":"38265_CR55","doi-asserted-by":"publisher","DOI":"10.1016\/j.impact.2024.100521","volume":"35","author":"N Ruijter","year":"2024","unstructured":"Ruijter N, van der Zee M, Katsumiti A, Boyles M, Cassee FR, Braakhuis H (2024) Improving the dichloro-dihydro-fluorescein (DCFH) assay for the assessment of intracellular reactive oxygen species formation by nanomaterials. NanoImpact 35:100521. https:\/\/doi.org\/10.1016\/j.impact.2024.100521","journal-title":"NanoImpact"},{"key":"38265_CR56","doi-asserted-by":"publisher","first-page":"1200","DOI":"10.1016\/j.ecoenv.2009.01.003","volume":"7","author":"SE Sabatini","year":"2009","unstructured":"Sabatini SE, Ju\u00e1rez AB, Eppis MR, Bianchi L, Luquet CM, R\u00edos de Molina MC (2009) Oxidative stress and antioxidant defenses in two green microalgae exposed to copper. Ecotoxicol Environ Saf 7:1200\u20131206. https:\/\/doi.org\/10.1016\/j.ecoenv.2009.01.003","journal-title":"Ecotoxicol Environ Saf"},{"key":"38265_CR57","doi-asserted-by":"publisher","unstructured":"S\u00e1enz ME, Di Marzio WD (2009) Ecotoxicity of herbicide Glyphosate to four chlorophyceaen freshwater algae. Limnetica 28:149\u2013158 (in Spanish). https:\/\/doi.org\/10.23818\/limn.28.11","DOI":"10.23818\/limn.28.11"},{"key":"38265_CR58","doi-asserted-by":"crossref","unstructured":"S\u00e1enz ME, Di Marzio WD, Alberdi JL, Tortorelli MC (1997) Effects of technical grade and commercial formulation of glyphosate on algal population growth. Bull Environ Contam Toxicol 59:638\u2013644.\u00a0https:\/\/doi.org\/10.1007\/s001289900527","DOI":"10.1007\/s001289900527"},{"key":"38265_CR59","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/0021-9673(96)00071-4","volume":"737","author":"JV Sancho","year":"1996","unstructured":"Sancho JV, Hernandez F, Lopez FJ, Hogendoorn EA, Dijkman E (1996) Rapid determination of glufosinate, glyphosate and aminomethylphosphonic acid in environmental water samples using precolumn fluorogenic labeling and coupled column liquid chromatography. J Chromatogr A 737:75\u201383. https:\/\/doi.org\/10.1016\/0021-9673(96)00071-4","journal-title":"J Chromatogr A"},{"key":"38265_CR60","unstructured":"Sasal MC, Wilson MG, Sione SM, Beghetto SM, Gabioud EA, Oszust JD, Paravani EV, Demonte L, Repetti MR, Bedendo DJ, Medero SL, Goette JJ, Pautasso N, Schulz GA (2017) Monitoring glyphosate in surface water in Entre R\u00edos. Participatory action research as an approach methodology. Revista de Investigaciones Agropecuarias 43(2):195\u2013205 (in Spanish).\u00a0https:\/\/www.scielo.org.ar\/scielo.php?script=sci_arttext&pid=S1669-23142017000200016&lng=es&nrm=iso&tlng=es"},{"key":"38265_CR61","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.pestbp.2006.01.001","volume":"85","author":"IG Sergiev","year":"2006","unstructured":"Sergiev IG, Alexieva VS, Ivanov SV, Moskova II, Karanov EN (2006) The phenylureacytokinin 4PU-30 protects maize plants against glyphosate action. Pestic Biochem Physiol 85:139\u2013146. https:\/\/doi.org\/10.1016\/j.pestbp.2006.01.001","journal-title":"Pestic Biochem Physiol"},{"key":"38265_CR62","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1111\/j.1432-1033.1993.tb18025.x","volume":"215","author":"H Sies","year":"1993","unstructured":"Sies H (1993) Strategies of antioxidant defense. Eur J Biochem 215:213\u2013219. https:\/\/doi.org\/10.1111\/j.1432-1033.1993.tb18025.x","journal-title":"Eur J Biochem"},{"key":"38265_CR63","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.redox.2013.01.007","volume":"1","author":"CM Spickett","year":"2013","unstructured":"Spickett CM (2013) The lipid peroxidation product 4-hydroxy-2-nonenal: advances in chemistry and analysis. Redox Biol 1:145\u2013152. https:\/\/doi.org\/10.1016\/j.redox.2013.01.007","journal-title":"Redox Biol"},{"key":"38265_CR64","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/s0021-9673(00)01009-8","volume":"907","author":"CD Stalikas","year":"2001","unstructured":"Stalikas CD, Konidari CN (2001) Analytical methods to determine phosphonic and amino acid group containing pesticides. J Chromatogr A 907:1\u201319. https:\/\/doi.org\/10.1016\/s0021-9673(00)01009-8","journal-title":"J Chromatogr A"},{"key":"38265_CR65","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.3390\/md9061101","volume":"9","author":"S Takaichi","year":"2011","unstructured":"Takaichi S (2011) Carotenoids in algae: distributions, biosyntheses and functions. Mar Drugs 9:1101\u20131118. https:\/\/doi.org\/10.3390\/md9061101","journal-title":"Mar Drugs"},{"key":"38265_CR66","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1006\/pest.1998.2319","volume":"59","author":"BD Tang","year":"1998","unstructured":"Tang BD, Siegfried KD, Hoagland KD (1998) Glutathione-S-transferase and in vitro metabolism of atrazine in freshwater algae. Pestic Biochem Physiol 59:155\u2013161. https:\/\/doi.org\/10.1006\/pest.1998.2319","journal-title":"Pestic Biochem Physiol"},{"key":"38265_CR67","doi-asserted-by":"crossref","unstructured":"Telfer A, Pascal A, Gall A (2008) Carotenoids in photosynthesis. In: Britton G, Liaaen-Jensen S, Pfander H (eds) Carotenoids, vol vol 4. Birkh\u00e4user Basel, pp 265\u2013308","DOI":"10.1007\/978-3-7643-7499-0_14"},{"key":"38265_CR68","doi-asserted-by":"crossref","unstructured":"Tsiaka P,\u00a0Tsarpali V,\u00a0Ntaikou\u00a0I,\u00a0Kostopoulou\u00a0MN,\u00a0Lyberatos\u00a0G, Dailianis S (2013) Carbamazepine-mediated pro-oxidant effects on the unicellular marine algal species\u00a0Dunaliella tertiolecta\u00a0and the hemocytes of mussel\u00a0Mytilus galloprovincialis. Ecotoxicoly\u00a022:1208\u20131220.\u00a0https:\/\/doi.org\/10.1007\/s10646-013-1108-3","DOI":"10.1007\/s10646-013-1108-3"},{"key":"38265_CR69","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1016\/S0045-6535(03)00306-0","volume":"52","author":"MT Tsui","year":"2003","unstructured":"Tsui MT, Chu LM (2003) Aquatic toxicity of glyphosate-based formulations: comparison between different organisms and the effects of environmental factors. Chemosphere 52:1189\u20131197. https:\/\/doi.org\/10.1016\/S0045-6535(03)00306-0","journal-title":"Chemosphere"},{"key":"38265_CR70","volume-title":"Glyphosate: herbicide information profile","author":"United States Department of Agriculture, Forest Service, Pacific Northwest Region","year":"2000","unstructured":"United States Department of Agriculture, Forest Service, Pacific Northwest Region (2000) Glyphosate: herbicide information profile. U.S. Department of Agriculture, Washington, DC, USA"},{"key":"38265_CR71","volume-title":"Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms","author":"United States Environmental Protection Agency","year":"2002","unstructured":"United States Environmental Protection Agency (2002) Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms, 4th edn. U.S. Environmental Protection Agency, Washington, DC, USA","edition":"4th edn"},{"key":"38265_CR72","doi-asserted-by":"publisher","unstructured":"Vallotton N, Moser D, Eggen RIL, Junghans M, Ch\u00e8vre N (2008) S-metolachlor pulse exposure on the algae Scenedesmus vacuolatus: effects during exposure and the subsequent recovery. Chemosphere 73:395\u2013400. https:\/\/doi.org\/10.1016\/j.chemosphere.2008.05.039","DOI":"10.1016\/j.chemosphere.2008.05.039"},{"key":"38265_CR73","doi-asserted-by":"publisher","first-page":"1447","DOI":"10.3390\/molecules28031447","volume":"28","author":"J Va\u0161kov\u00e1","year":"2023","unstructured":"Va\u0161kov\u00e1 J, Ko\u010dan L, Va\u0161ko L, Perj\u00e9si P (2023) Glutathione-related enzymes and proteins: a review. Molecules 28:1447. https:\/\/doi.org\/10.3390\/molecules28031447","journal-title":"Molecules"},{"key":"38265_CR74","doi-asserted-by":"publisher","first-page":"233","DOI":"10.1016\/S1011-1344(98)00076-1","volume":"42","author":"DV Vavilin","year":"1998","unstructured":"Vavilin DV, Ducruet JM, Matorin DN, Venediktov PS, Rubin AB (1998) Membrane lipid peroxidation, cell viability and photosystem II activity in the green alga Chlorella pyrenoidosa subjected to various stress conditions. J Photochem Photobiol B 42:233\u2013239. https:\/\/doi.org\/10.1016\/S1011-1344(98)00076-1","journal-title":"J Photochem Photobiol B"},{"key":"38265_CR75","first-page":"167","volume-title":"Phytoplankton manual, monographs on oceanographic methodology","author":"EL Venrick","year":"1978","unstructured":"Venrick EL (1978) How many cells to count? In: Sournia A (ed) Phytoplankton manual, monographs on oceanographic methodology. UNESCO Press, Paris, pp 167\u2013180"},{"key":"38265_CR76","doi-asserted-by":"publisher","first-page":"612","DOI":"10.1016\/S0891-5849(99)00107-0","volume":"27","author":"H Wang","year":"1999","unstructured":"Wang H, Joseph JA (1999) Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader. Free Radic Biol Med 27:612\u2013616. https:\/\/doi.org\/10.1016\/S0891-5849(99)00107-0","journal-title":"Free Radic Biol Med"},{"key":"38265_CR77","first-page":"56","volume":"36","author":"E Will\u00e9n","year":"2000","unstructured":"Will\u00e9n E (2000) Phytoplankton in water quality assessment \u2014 an indicator concept. Phycologia 36:56\u201380.\u00a0https:\/\/doi.org\/10.1002\/9780470511121.ch6","journal-title":"Phycologia"},{"key":"38265_CR78","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/S0045-6535(99)00408-7","volume":"41","author":"PK Wong","year":"2000","unstructured":"Wong PK (2000) Effects of 2,4-D, glyphosate and paraquat on growth, photosynthesis and chlorophyll-a synthesis of Scenedesmus quadricauda Berb 614. Chemosphere 41:177\u2013182. https:\/\/doi.org\/10.1016\/S0045-6535(99)00408-7","journal-title":"Chemosphere"},{"key":"38265_CR79","doi-asserted-by":"publisher","first-page":"4225","DOI":"10.1529\/biophysj.107.112565","volume":"93","author":"J Wong-Ekkabut","year":"2007","unstructured":"Wong-Ekkabut J, Xu Z, Triampo W, Tang I-M, Tieleman DP, Monticelli L (2007) Effect of lipid peroxidation on the properties of lipid bilayers: a molecular dynamics study. Biophys J 93:4225\u20134236. https:\/\/doi.org\/10.1529\/biophysj.107.112565","journal-title":"Biophys J"},{"key":"38265_CR80","doi-asserted-by":"publisher","first-page":"374","DOI":"10.3109\/10715762.2015.1014813","volume":"49","author":"X Zhang","year":"2015","unstructured":"Zhang X, Gao F (2015) Imaging mitochondrial reactive oxygen species with fluorescent probes: current applications and challenges. Free Radic Res 49:374\u2013382. https:\/\/doi.org\/10.3109\/10715762.2015.1014813","journal-title":"Free Radic Res"}],"container-title":["Environmental Science and Pollution Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11356-026-38265-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11356-026-38265-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11356-026-38265-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:03:57Z","timestamp":1792440237000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11356-026-38265-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,5]]},"references-count":80,"journal-issue":{"issue":"34","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["38265"],"URL":"https:\/\/doi.org\/10.1007\/s11356-026-38265-y","relation":{},"ISSN":["1614-7499"],"issn-type":[{"value":"1614-7499","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,5]]},"assertion":[{"value":"6 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 September 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"5 October 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"This is not applicable.","order":1,"name":"Ethics","label":"Ethical approval","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This is not applicable.","order":2,"name":"Ethics","label":"Consent to participate","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This is not applicable.","order":3,"name":"Ethics","label":"Consent to publication","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":4,"name":"Ethics","label":"Competing interests","group":{"name":"EthicsHeading","label":"Declarations"}}]}}