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Traditional approaches for measurement of muscle protein turnover require infusion of expensive, sterile, isotopically labelled tracers which limits the applicability of these approaches in certain populations (e.g. clinical, frail elderly). To concurrently quantify skeletal muscle mass and muscle protein turnover i.e. muscle protein synthesis (MPS) and muscle protein breakdown (MPB), in elderly human volunteers using stable-isotope labelled tracers i.e. Methyl-[D<jats:sub>3<\/jats:sub>]-creatine (D<jats:sub>3<\/jats:sub>-Cr), deuterium oxide (D<jats:sub>2<\/jats:sub>O), and Methyl-[D<jats:sub>3<\/jats:sub>]-3-methylhistidine (D<jats:sub>3<\/jats:sub>-3MH), to measure muscle mass, MPS and MPB, respectively. We recruited 10 older males (71\u2009\u00b1\u20094 y, BMI: 25\u2009\u00b1\u20094\u00a0kg<jats:sup>.<\/jats:sup>m<jats:sup>2<\/jats:sup>, mean\u2009\u00b1\u2009SD) into a 4-day study, with DXA and consumption of D<jats:sub>2<\/jats:sub>O and D<jats:sub>3<\/jats:sub>-Cr tracers on day 1. D<jats:sub>3<\/jats:sub>-3MH was consumed on day 3, 24\u00a0h prior to returning to the lab. From urine, saliva and blood samples, and a single muscle biopsy (vastus lateralis), we determined muscle mass, MPS and MPB. D<jats:sub>3<\/jats:sub>-Cr derived muscle mass was positively correlated to appendicular fat-free mass (AFFM) estimated by DXA (r\u2009=\u20090.69, P\u2009=\u20090.027). Rates of cumulative myofibrillar MPS over 3\u00a0days were 0.072%\/h (95% CI, 0.064 to 0.081%\/h). Whole-body MPB over 6\u00a0h was 0.052 (95% CI, 0.038 to 0.067). These rates were similar to previous literature. We demonstrate the potential for D<jats:sub>3<\/jats:sub>-Cr to be used alongside D<jats:sub>2<\/jats:sub>O and D<jats:sub>3<\/jats:sub>-3MH for concurrent measurement of muscle mass, MPS, and MPB using a minimally invasive design, applicable for clinical and frail populations.<\/jats:p>","DOI":"10.1007\/s11357-021-00386-2","type":"journal-article","created":{"date-parts":[[2021,5,27]],"date-time":"2021-05-27T21:02:37Z","timestamp":1622149357000},"page":"2653-2665","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Combined in vivo muscle mass, muscle protein synthesis and muscle protein breakdown measurement: a \u2018Combined Oral Stable Isotope Assessment of Muscle (COSIAM)\u2019 approach"],"prefix":"10.1007","volume":"43","author":[{"given":"Jessica","family":"Cegielski","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel J.","family":"Wilkinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew S.","family":"Brook","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Catherine","family":"Boereboom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bethan E.","family":"Phillips","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F. 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