{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:04:23Z","timestamp":1791234263167,"version":"4.1.0"},"reference-count":71,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2021,9,18]],"date-time":"2021-09-18T00:00:00Z","timestamp":1631923200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,9,18]],"date-time":"2021-09-18T00:00:00Z","timestamp":1631923200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["P30AG013280"],"award-info":[{"award-number":["P30AG013280"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["R01AG061132"],"award-info":[{"award-number":["R01AG061132"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000049","name":"National Institute on Aging","doi-asserted-by":"publisher","award":["F32AG054098"],"award-info":[{"award-number":["F32AG054098"]}],"id":[{"id":"10.13039\/100000049","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["GeroScience"],"published-print":{"date-parts":[[2021,10]]},"DOI":"10.1007\/s11357-021-00436-9","type":"journal-article","created":{"date-parts":[[2021,9,18]],"date-time":"2021-09-18T08:02:51Z","timestamp":1631952171000},"page":"2621-2631","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Generation and characterization of a tractable C. elegans model of tauopathy"],"prefix":"10.1007","volume":"43","author":[{"given":"Joshua C.","family":"Russell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haoyi","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul K.","family":"Chaliparambil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Fish","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan M.","family":"Markiewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-I.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anushka","family":"Noori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1311-3421","authenticated-orcid":false,"given":"Matt","family":"Kaeberlein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,9,18]]},"reference":[{"key":"436_CR1","doi-asserted-by":"crossref","first-page":"1121","DOI":"10.1146\/annurev.neuro.24.1.1121","volume":"24","author":"VM Lee","year":"2001","unstructured":"Lee VM, Goedert M, Trojanowski JQ. Neurodegenerative tauopathies. Annu Rev Neurosci. 2001;24:1121\u201359.","journal-title":"Annu Rev Neurosci"},{"key":"436_CR2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1111\/nan.12208","volume":"41","author":"GG Kovacs","year":"2015","unstructured":"Kovacs GG. Invited review: neuropathology of tauopathies: principles and practice. Neuropathol Appl Neurobiol. 2015;41:3\u201323.","journal-title":"Neuropathol Appl Neurobiol"},{"key":"436_CR3","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1016\/j.bcp.2013.12.024","volume":"88","author":"C Reitz","year":"2014","unstructured":"Reitz C, Mayeux R. Alzheimer disease: epidemiology, diagnostic criteria, risk factors and biomarkers. Biochem Pharmacol. 2014;88:640\u201351.","journal-title":"Biochem Pharmacol"},{"key":"436_CR4","first-page":"679","volume":"62","author":"T Ikeda","year":"2010","unstructured":"Ikeda T, Yamada M. Risk factors for Alzheimer\u2019s disease. Brain Nerve. 2010;62:679\u201390.","journal-title":"Brain Nerve"},{"key":"436_CR5","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1093\/ppar\/prz020","volume":"29","author":"M Kaeberlein","year":"2019","unstructured":"Kaeberlein M. Time for a new strategy in the war on Alzheimer\u2019s disease. Public Policy & Aging Report. 2019;29:119\u201322.","journal-title":"Public Policy & Aging Report"},{"key":"436_CR6","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.cell.2013.05.039","volume":"153","author":"C Lopez-Otin","year":"2013","unstructured":"Lopez-Otin C, Blasco MA, Partridge L, Serrano M, Kroemer G. The hallmarks of aging. Cell. 2013;153:1194\u2013217.","journal-title":"Cell"},{"key":"436_CR7","doi-asserted-by":"crossref","unstructured":"M. Kaeberlein, V. Galvan, Rapamycin and Alzheimer's disease: time for a clinical trial? Sci Transl Med 11 (2019)","DOI":"10.1126\/scitranslmed.aar4289"},{"key":"436_CR8","doi-asserted-by":"crossref","first-page":"53","DOI":"10.29245\/2572.942X\/2016\/6.1059","volume":"1","author":"JF Crary","year":"2016","unstructured":"Crary JF. Primary age-related tauopathy and the amyloid cascade hypothesis: the exception that proves the rule? J Neurol Neuromedicine. 2016;1:53\u20137.","journal-title":"J Neurol Neuromedicine"},{"key":"436_CR9","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s00401-011-0910-3","volume":"123","author":"TJ Montine","year":"2012","unstructured":"Montine TJ, et al. National Institute on Aging-Alzheimer\u2019s Association guidelines for the neuropathologic assessment of Alzheimer\u2019s disease: a practical approach. Acta Neuropathol. 2012;123:1\u201311.","journal-title":"Acta Neuropathol"},{"key":"436_CR10","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1016\/j.jalz.2015.07.492","volume":"12","author":"TJ Montine","year":"2016","unstructured":"Montine TJ, et al. Multisite assessment of NIA-AA guidelines for the neuropathologic evaluation of Alzheimer\u2019s disease. Alzheimers Dement. 2016;12:164\u20139.","journal-title":"Alzheimers Dement"},{"key":"436_CR11","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1007\/s00401-009-0486-3","volume":"118","author":"K Iqbal","year":"2009","unstructured":"Iqbal K, Liu F, Gong CX, Alonso AdC, Grundke-Iqbal I. Mechanisms of tau-induced neurodegeneration. Acta neuropathologica. 2009;118:53\u201369.","journal-title":"Acta neuropathologica"},{"key":"436_CR12","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1007\/BF00308809","volume":"82","author":"H Braak","year":"1991","unstructured":"Braak H, Braak E. Neuropathological stageing of Alzheimer-related changes. Acta Neuropathol. 1991;82:239\u201359.","journal-title":"Acta Neuropathol"},{"key":"436_CR13","doi-asserted-by":"crossref","first-page":"1791","DOI":"10.1212\/WNL.58.12.1791","volume":"58","author":"DR Thal","year":"2002","unstructured":"Thal DR, R\u00fcb U, Orantes M, Braak H. Phases of A\u03b2-deposition in the human brain and its relevance for the development of AD. Neurology. 2002;58:1791\u2013800.","journal-title":"Neurology"},{"key":"436_CR14","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/S0165-0173(00)00019-9","volume":"33","author":"L Bu\u00e9e","year":"2000","unstructured":"Bu\u00e9e L, Bussi\u00e8re T, Bu\u00e9e-Scherrer V, Delacourte A, Hof PR. Tau protein isoforms, phosphorylation and role in neurodegenerative disorders. Brain Res Rev. 2000;33:95\u2013130.","journal-title":"Brain Res Rev"},{"key":"436_CR15","doi-asserted-by":"crossref","first-page":"6","DOI":"10.3390\/biom6010006","volume":"6","author":"G \u0160imi\u0107","year":"2016","unstructured":"\u0160imi\u0107 G, et al. Tau protein hyperphosphorylation and aggregation in Alzheimer\u2019s disease and other tauopathies, and possible neuroprotective strategies. Biomolecules. 2016;6:6.","journal-title":"Biomolecules"},{"key":"436_CR16","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1002\/path.2440","volume":"216","author":"F Pont\u00e9n","year":"2008","unstructured":"Pont\u00e9n F, Jirstr\u00f6m K, Uhlen M. The Human Protein Atlas\u2014a tool for pathology. The Journal of Pathology: A Journal of the Pathological Society of Great Britain and Ireland. 2008;216:387\u201393.","journal-title":"The Journal of Pathology: A Journal of the Pathological Society of Great Britain and Ireland"},{"key":"436_CR17","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1111\/j.1365-2990.2004.00577.x","volume":"30","author":"CA Maurage","year":"2004","unstructured":"Maurage CA, et al. Tau aggregates are abnormally phosphorylated in inclusion body myositis and have an immunoelectrophoretic profile distinct from other tauopathies. Neuropathol Appl Neurobiol. 2004;30:624\u201334.","journal-title":"Neuropathol Appl Neurobiol"},{"key":"436_CR18","first-page":"177","volume":"144","author":"V Askanas","year":"1994","unstructured":"Askanas V, Engel WK, Bilak M, Alvarez RB, Selkoe DJ. Twisted tubulofilaments of inclusion body myositis muscle resemble paired helical filaments of Alzheimer brain and contain hyperphosphorylated tau. Am J Pathol. 1994;144:177\u201387.","journal-title":"Am J Pathol"},{"key":"436_CR19","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/s00401-008-0449-0","volume":"116","author":"V Askanas","year":"2008","unstructured":"Askanas V, Engel WK. Inclusion-body myositis: muscle-fiber molecular pathology and possible pathogenic significance of its similarity to Alzheimer\u2019s and Parkinson\u2019s disease brains. Acta Neuropathol. 2008;116:583\u201395.","journal-title":"Acta Neuropathol"},{"key":"436_CR20","doi-asserted-by":"crossref","unstructured":"D. Levacic, L. R. Peddareddygari, D. Nochlin, L. R. Sharer, R. P. Grewal, Inclusion-body myositis associated with Alzheimer\u2019s disease. Case reports in medicine 2013 (2013)","DOI":"10.1155\/2013\/536231"},{"key":"436_CR21","first-page":"74","volume":"5","author":"QY Liu","year":"2016","unstructured":"Liu QY, et al. Molecular events linking cholesterol to Alzheimer\u2019s disease and inclusion body myositis in a rabbit model. Am J Neurodegener Dis. 2016;5:74.","journal-title":"Am J Neurodegener Dis"},{"key":"436_CR22","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.neulet.2007.11.066","volume":"431","author":"TJ Kannanayakal","year":"2008","unstructured":"Kannanayakal TJ, Mendell JR, Kuret J. Casein kinase 1 alpha associates with the tau-bearing lesions of inclusion body myositis. Neurosci Lett. 2008;431:141\u20135.","journal-title":"Neurosci Lett"},{"key":"436_CR23","doi-asserted-by":"crossref","unstructured":"D. Paul, S. Chipurupalli, A. Justin, K. Raja, S. K. Mohankumar, Caenorhabditis elegans as a possible model to screen anti-Alzheimer's therapeutics. Journal of Pharmacological and Toxicological Methods, 106932 (2020)","DOI":"10.1016\/j.vascn.2020.106932"},{"key":"436_CR24","doi-asserted-by":"crossref","first-page":"e0219486","DOI":"10.1371\/journal.pone.0219486","volume":"14","author":"R Godini","year":"2019","unstructured":"Godini R, Pocock R, Fallahi H. Caenorhabditis elegans hub genes that respond to amyloid beta are homologs of genes involved in human Alzheimer\u2019s disease. Plos one. 2019;14:e0219486.","journal-title":"Plos one"},{"key":"436_CR25","doi-asserted-by":"crossref","first-page":"2596","DOI":"10.1021\/acschemneuro.7b00361","volume":"8","author":"EF Griffin","year":"2017","unstructured":"Griffin EF, Caldwell KA, Caldwell GA. Genetic and pharmacological discovery for Alzheimer\u2019s disease using Caenorhabditis elegans. ACS Chem Neurosci. 2017;8:2596\u2013606.","journal-title":"ACS Chem Neurosci"},{"key":"436_CR26","doi-asserted-by":"crossref","first-page":"279","DOI":"10.3389\/fgene.2014.00279","volume":"5","author":"AG Alexander","year":"2014","unstructured":"Alexander AG, Marfil V, Li C. Use of Caenorhabditis elegans as a model to study Alzheimer\u2019s disease and other neurodegenerative diseases. Front Genet. 2014;5:279.","journal-title":"Front Genet"},{"key":"436_CR27","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1007\/s00429-009-0235-3","volume":"214","author":"CY Ewald","year":"2010","unstructured":"Ewald CY, Li C. Understanding the molecular basis of Alzheimer\u2019s disease using a Caenorhabditis elegans model system. Brain Struct Funct. 2010;214:263\u201383.","journal-title":"Brain Struct Funct"},{"key":"436_CR28","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0378-1119(00)00579-5","volume":"263","author":"L Timmons","year":"2001","unstructured":"Timmons L, Court DL, Fire A. Ingestion of bacterially expressed dsRNAs can produce specific and potent genetic interference in Caenorhabditis elegans. Gene. 2001;263:103\u201312.","journal-title":"Gene"},{"key":"436_CR29","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S1046-2023(03)00050-1","volume":"30","author":"RS Kamath","year":"2003","unstructured":"Kamath RS, Ahringer J. Genome-wide RNAi screening in Caenorhabditis elegans. Methods. 2003;30:313\u201321.","journal-title":"Methods"},{"key":"436_CR30","doi-asserted-by":"crossref","first-page":"9980","DOI":"10.1073\/pnas.1533448100","volume":"100","author":"BC Kraemer","year":"2003","unstructured":"Kraemer BC, et al. Neurodegeneration and defective neurotransmission in a Caenorhabditis elegans model of tauopathy. Proc Natl Acad Sci. 2003;100:9980\u20135.","journal-title":"Proc Natl Acad Sci"},{"key":"436_CR31","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1016\/j.nbd.2005.03.017","volume":"20","author":"T Miyasaka","year":"2005","unstructured":"Miyasaka T, et al. Progressive neurodegeneration in C. elegans model of tauopathy. Neurobiology of disease. 2005;20:372\u201383.","journal-title":"Neurobiology of disease"},{"key":"436_CR32","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.neurobiolaging.2007.05.011","volume":"30","author":"R Brandt","year":"2009","unstructured":"Brandt R, Gergou A, Wacker I, Fath T, Hutter H. A Caenorhabditis elegans model of tau hyperphosphorylation: induction of developmental defects by transgenic overexpression of Alzheimer\u2019s disease-like modified tau. Neurobiol Aging. 2009;30:22\u201333.","journal-title":"Neurobiol Aging"},{"key":"436_CR33","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13024-015-0066-z","volume":"11","author":"GJ Pir","year":"2016","unstructured":"Pir GJ, Choudhary B, Mandelkow E, Mandelkow E-M. Tau mutant A152T, a risk factor for FTD\/PSP, induces neuronal dysfunction and reduced lifespan independently of aggregation in a C. elegans Tauopathy model. Molecular neurodegeneration. 2016;11:1\u201321.","journal-title":"Molecular neurodegeneration"},{"key":"436_CR34","doi-asserted-by":"crossref","first-page":"1498","DOI":"10.1093\/hmg\/ddy442","volume":"28","author":"VJ Butler","year":"2019","unstructured":"Butler VJ, et al. Tau\/MAPT disease-associated variant A152T alters tau function and toxicity via impaired retrograde axonal transport. Hum Mol Genet. 2019;28:1498\u2013514.","journal-title":"Hum Mol Genet"},{"key":"436_CR35","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.neurobiolaging.2015.11.004","volume":"39","author":"T Miyasaka","year":"2016","unstructured":"Miyasaka T, et al. Curcumin improves tau-induced neuronal dysfunction of nematodes. Neurobiol Aging. 2016;39:69\u201381.","journal-title":"Neurobiol Aging"},{"key":"436_CR36","doi-asserted-by":"crossref","first-page":"226","DOI":"10.1016\/j.nbd.2018.06.018","volume":"117","author":"F Morelli","year":"2018","unstructured":"Morelli F, et al. V363I and V363A mutated tau affect aggregation and neuronal dysfunction differently in C. elegans. Neurobiology of disease. 2018;117:226\u201334.","journal-title":"Neurobiology of disease"},{"key":"436_CR37","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1038\/nature01278","volume":"421","author":"RS Kamath","year":"2003","unstructured":"Kamath RS, et al. Systematic functional analysis of the Caenorhabditis elegans genome using RNAi. Nature. 2003;421:231\u20137.","journal-title":"Nature"},{"key":"436_CR38","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1126\/science.1087117","volume":"301","author":"EH Feinberg","year":"2003","unstructured":"Feinberg EH, Hunter CP. Transport of dsRNA into cells by the transmembrane protein SID-1. Science. 2003;301:1545\u20137.","journal-title":"Science"},{"key":"436_CR39","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1093\/genetics\/135.2.385","volume":"135","author":"PG Okkema","year":"1993","unstructured":"Okkema PG, Harrison SW, Plunger V, Aryana A, Fire A. Sequence requirements for myosin gene expression and regulation in Caenorhabditis elegans. Genetics. 1993;135:385\u2013404.","journal-title":"Genetics"},{"key":"436_CR40","doi-asserted-by":"crossref","unstructured":"L. Wu et al., Human tau isoform aggregation and selective detection of misfolded tau from post-mortem Alzheimer\u2019s disease brains. bioRxiv, 2020. 2019.2012. 2031.876946","DOI":"10.1101\/2019.12.31.876946"},{"key":"436_CR41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41467-019-13993-7","volume":"11","author":"Z He","year":"2020","unstructured":"He Z, et al. Transmission of tauopathy strains is independent of their isoform composition. Nat Commun. 2020;11:1\u201318.","journal-title":"Nat Commun"},{"key":"436_CR42","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/0012-1606(76)90119-6","volume":"51","author":"L Byerly","year":"1976","unstructured":"Byerly L, Cassada R, Russell R. The life cycle of the nematode Caenorhabditis elegans: I Wild-type growth and reproduction. Developmental biology. 1976;51:23\u201333.","journal-title":"Developmental biology"},{"key":"436_CR43","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/1750-1326-7-57","volume":"7","author":"G McColl","year":"2012","unstructured":"McColl G, et al. Utility of an improved model of amyloid-beta (A\u03b2 1\u201342) toxicity in Caenorhabditis elegans for drug screening for Alzheimer\u2019s disease. Mol Neurodegener. 2012;7:1\u20139.","journal-title":"Mol Neurodegener"},{"key":"436_CR44","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1038\/nature25143","volume":"552","author":"V Sorrentino","year":"2017","unstructured":"Sorrentino V, et al. Enhancing mitochondrial proteostasis reduces amyloid-\u03b2 proteotoxicity. Nature. 2017;552:187\u201393.","journal-title":"Nature"},{"key":"436_CR45","doi-asserted-by":"crossref","first-page":"1133","DOI":"10.1016\/j.jalz.2017.01.016","volume":"13","author":"S Mukherjee","year":"2017","unstructured":"Mukherjee S, et al. Systems biology approach to late-onset Alzheimer\u2019s disease genome-wide association study identifies novel candidate genes validated using brain expression data and Caenorhabditis elegans experiments. Alzheimers Dement. 2017;13:1133\u201342.","journal-title":"Alzheimers Dement"},{"key":"436_CR46","doi-asserted-by":"crossref","first-page":"138","DOI":"10.3389\/fnagi.2017.00138","volume":"9","author":"N Groh","year":"2017","unstructured":"Groh N, et al. Age-dependent protein aggregation initiates amyloid-\u03b2 aggregation. Frontiers in aging neuroscience. 2017;9:138.","journal-title":"Frontiers in aging neuroscience"},{"key":"436_CR47","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1111\/j.1474-9726.2008.00385.x","volume":"7","author":"KA Steinkraus","year":"2008","unstructured":"Steinkraus KA, et al. Dietary restriction suppresses proteotoxicity and enhances longevity by an hsf-1-dependent mechanism in Caenorhabditis elegans. Aging Cell. 2008;7:394\u2013404.","journal-title":"Aging Cell"},{"key":"436_CR48","doi-asserted-by":"crossref","first-page":"1196","DOI":"10.1126\/science.1173507","volume":"324","author":"R Mehta","year":"2009","unstructured":"Mehta R, et al. Proteasomal regulation of the hypoxic response modulates aging in C. elegans. Science. 2009;324:1196\u20138.","journal-title":"Science"},{"key":"436_CR49","doi-asserted-by":"crossref","first-page":"225a","DOI":"10.1016\/j.bpj.2017.11.1253","volume":"114","author":"S Chia","year":"2018","unstructured":"Chia S, et al. Systematic development of small molecules to inhibit specific microscopic steps of amyloid-beta42 aggregation in Alzheimer\u2019s disease. Biophys J . 2018;114:225a.","journal-title":"Biophys J"},{"key":"436_CR50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s13065-014-0076-x","volume":"9","author":"X Chen","year":"2015","unstructured":"Chen X, Barclay JW, Burgoyne RD, Morgan A. Using C. elegans to discover therapeutic compounds for ageing-associated neurodegenerative diseases. Chemistry Central Journal. 2015;9:1\u201320.","journal-title":"Chemistry Central Journal"},{"key":"436_CR51","first-page":"2195","volume":"9","author":"G Genes","year":"2019","unstructured":"Genes G. C. G. Silva-Garc\u00eda et al., Single-copy knock-in loci for defined gene expression in Caenorhabditis elegans. G3. Genetics. 2019;9:2195\u20138.","journal-title":"Genetics"},{"key":"436_CR52","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1016\/j.gene.2007.06.020","volume":"400","author":"H Qadota","year":"2007","unstructured":"Qadota H, et al. Establishment of a tissue-specific RNAi system in C. elegans. Gene. 2007;400:166\u201373.","journal-title":"Gene"},{"key":"436_CR53","first-page":"4167","volume":"10","author":"G Genes","year":"2020","unstructured":"Genes G. J. S. Watts et al., New strains for tissue-specific RNAi studies in Caenorhabditis elegans. G3. Genetics. 2020;10:4167\u201376.","journal-title":"Genetics"},{"key":"436_CR54","doi-asserted-by":"crossref","first-page":"e1000028","DOI":"10.1371\/journal.pgen.1000028","volume":"4","author":"MW Davis","year":"2008","unstructured":"Davis MW, Morton JJ, Carroll D, Jorgensen EM. Gene activation using FLP recombinase in C. elegans. PLoS Genet. 2008;4:e1000028.","journal-title":"PLoS Genet"},{"key":"436_CR55","doi-asserted-by":"publisher","DOI":"10.1007\/s12035-020-02084-0","author":"S Guha","year":"2020","unstructured":"Guha S, Johnson GVW, Nehrke K. The crosstalk between pathological tau phosphorylation and mitochondrial dysfunction as a key to understanding and treating Alzheimer\u2019s disease. Mol Neurobiol. 2020. https:\/\/doi.org\/10.1007\/s12035-020-02084-0.","journal-title":"Mol Neurobiol"},{"key":"436_CR56","doi-asserted-by":"crossref","first-page":"44","DOI":"10.3389\/fnins.2018.00044","volume":"12","author":"T Kimura","year":"2018","unstructured":"Kimura T, Sharma G, Ishiguro K, Hisanaga S-I. Phospho-tau bar code: analysis of phosphoisotypes of tau and its application to tauopathy. Front Neurosci. 2018;12:44.","journal-title":"Front Neurosci"},{"key":"436_CR57","doi-asserted-by":"crossref","first-page":"83","DOI":"10.3389\/fneur.2013.00083","volume":"4","author":"W Noble","year":"2013","unstructured":"Noble W, Hanger DP, Miller CC, Lovestone S. The importance of tau phosphorylation for neurodegenerative diseases. Front Neurol. 2013;4:83.","journal-title":"Front Neurol"},{"key":"436_CR58","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1007\/s11357-019-00124-9","volume":"41","author":"JN Pitt","year":"2019","unstructured":"Pitt JN, et al. WormBot, an open-source robotics platform for survival and behavior analysis in C. elegans. GeroScience. 2019;41:961\u201373.","journal-title":"GeroScience"},{"key":"436_CR59","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1093\/hmg\/ddz319","volume":"29","author":"SJ Benbow","year":"2020","unstructured":"Benbow SJ, Strovas TJ, Darvas M, Saxton A, Kraemer BC. Synergistic toxicity between tau and amyloid drives neuronal dysfunction and neurodegeneration in transgenic C. elegans. Human molecular genetics. 2020;29:495\u2013505.","journal-title":"Human molecular genetics"},{"key":"436_CR60","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.nbd.2017.10.003","volume":"109","author":"C Wang","year":"2018","unstructured":"Wang C, Saar V, Leung KL, Chen L, Wong G. Human amyloid \u03b2 peptide and tau co-expression impairs behavior and causes specific gene expression changes in Caenorhabditis elegans. Neurobiol Dis. 2018;109:88\u2013101.","journal-title":"Neurobiol Dis"},{"key":"436_CR61","doi-asserted-by":"crossref","first-page":"e1000027","DOI":"10.1371\/journal.pgen.1000027","volume":"4","author":"TJ Van Ham","year":"2008","unstructured":"Van Ham TJ, et al. C. elegans model identifies genetic modifiers of \u03b1-synuclein inclusion formation during aging. PLoS Genet. 2008;4:e1000027.","journal-title":"PLoS Genet."},{"key":"436_CR62","doi-asserted-by":"crossref","first-page":"e0173644","DOI":"10.1371\/journal.pone.0173644","volume":"12","author":"AL Lee","year":"2017","unstructured":"Lee AL, Ung HM, Sands LP, Kikis EA. A new Caenorhabditis elegans model of human huntingtin 513 aggregation and toxicity in body wall muscles. PloS one. 2017;12:e0173644.","journal-title":"PloS one."},{"key":"436_CR63","doi-asserted-by":"crossref","first-page":"21","DOI":"10.4161\/auto.3528","volume":"3","author":"K Jia","year":"2007","unstructured":"Jia K, Hart AC, Levine B. Autophagy genes protect against disease caused by polyglutamine expansion proteins in Caenorhabditis elegans. Autophagy. 2007;3:21\u20135.","journal-title":"Autophagy"},{"key":"436_CR64","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1093\/genetics\/77.1.71","volume":"77","author":"S Brenner","year":"1974","unstructured":"Brenner S. The genetics of Caenorhabditis elegans. Genetics. 1974;77:71\u201394.","journal-title":"Genetics"},{"key":"436_CR65","doi-asserted-by":"crossref","first-page":"W597","DOI":"10.1093\/nar\/gks400","volume":"40","author":"P Artimo","year":"2012","unstructured":"Artimo P, et al. ExPASy: SIB bioinformatics resource portal. Nucleic Acids Res. 2012;40:W597\u2013603.","journal-title":"Nucleic Acids Res"},{"key":"436_CR66","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1038\/nmeth.1565","volume":"8","author":"S Redemann","year":"2011","unstructured":"Redemann S, et al. Codon adaptation\u2013based control of protein expression in C. elegans. Nature methods. 2011;8:250\u20132.","journal-title":"Nature methods"},{"key":"436_CR67","doi-asserted-by":"crossref","first-page":"2162","DOI":"10.1101\/gr.2505604","volume":"14","author":"J-F Rual","year":"2004","unstructured":"Rual J-F, et al. Toward improving Caenorhabditis elegans phenome mapping with an ORFeome-based RNAi library. Genome Res. 2004;14:2162\u20138.","journal-title":"Genome Res"},{"key":"436_CR68","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin J, et al. Fiji: an open-source platform for biological-image analysis. Nat Methods. 2012;9:676\u201382.","journal-title":"Nat Methods"},{"key":"436_CR69","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1017\/S1431927619001442","volume":"25","author":"CT Rueden","year":"2019","unstructured":"Rueden CT, Eliceiri KW. ImageJ for the next generation of scientific image data. Microsc Microanal. 2019;25:142\u20133.","journal-title":"Microsc Microanal"},{"key":"436_CR70","doi-asserted-by":"crossref","unstructured":"M. Koopman, R. I. Seinstra, E. A. Nollen, C. elegans as a model for synucleinopathies and other neurodegenerative diseases: tools and techniques in Alpha-Synuclein. (Springer, 2019), pp. 93\u2013112","DOI":"10.1007\/978-1-4939-9124-2_9"},{"key":"436_CR71","doi-asserted-by":"crossref","unstructured":"G. L. Sutphin, M. Kaeberlein, Measuring Caenorhabditis elegans life span on solid media. Journal of visualized experiments: JoVE (2009)","DOI":"10.3791\/1152"}],"container-title":["GeroScience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11357-021-00436-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11357-021-00436-9\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11357-021-00436-9.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,1,9]],"date-time":"2023-01-09T16:50:28Z","timestamp":1673283028000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11357-021-00436-9"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,9,18]]},"references-count":71,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,10]]}},"alternative-id":["436"],"URL":"https:\/\/doi.org\/10.1007\/s11357-021-00436-9","relation":{},"ISSN":["2509-2715","2509-2723"],"issn-type":[{"value":"2509-2715","type":"print"},{"value":"2509-2723","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,9,18]]},"assertion":[{"value":"6 April 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 August 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 September 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}