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Here, we show that frontal cortex samples from brains of cognitively normal aging humans had progressively increased levels of soluble amyloid peptide A\u03b240 throughout the lifespan. Aggregated amyloid fraction was subsequently obtained by formic acid, where A\u03b242 showed increases only in humans over 90\u00a0years old when compared to those younger than 50. Similarly, aging wild-type mice without amyloid plaques had increases of both soluble A\u03b240 and A\u03b242, as previously shown in normal aging rats. Aging also alters secretase enzymes and processing of amyloid precursor protein (APP). Here, we isolate membrane domains known as lipid rafts, a site of APP cleavage. We found that lipid rafts isolated from mouse and human cerebral cortex showed age increases of \u03b2-secretase enzyme activity, while amyloidogenic secretase proteins levels BACE1 and PS1 decreased with age in mouse. 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