{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T16:30:00Z","timestamp":1793464200796,"version":"4.5.0"},"reference-count":28,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100007173","name":"Bio-oriented Technology Research Advancement Institution","doi-asserted-by":"publisher","award":["JPJ011937"],"award-info":[{"award-number":["JPJ011937"]}],"id":[{"id":"10.13039\/501100007173","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["J Nat Med"],"published-print":{"date-parts":[[2026,11]]},"DOI":"10.1007\/s11418-026-02072-z","type":"journal-article","created":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T12:09:49Z","timestamp":1789474189000},"page":"1638-1652","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rhizobial inoculation promotes glycyrrhizic acid production through jasmonic acid signaling in Glycyrrhiza uralensis"],"prefix":"10.1007","volume":"80","author":[{"given":"Shion","family":"Yamamoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Shimomura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoshi","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nazmul","family":"Hasan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"2072_CR1","doi-asserted-by":"publisher","first-page":"e02014","DOI":"10.1016\/j.gecco.2022.e02014","volume":"34","author":"Y Han","year":"2022","unstructured":"Han Y, Pang X, Zhang X, Han R, Liang Z (2022) Resource sustainability and challenges: Status and competitiveness of international trade in licorice extracts under the Belt and Road Initiative. Global Ecol Conserv 34:e02014. https:\/\/doi.org\/10.1016\/j.gecco.2022.e02014","journal-title":"Global Ecol Conserv"},{"key":"2072_CR2","first-page":"76","volume":"64","author":"K Ozaki","year":"2010","unstructured":"Ozaki K, Shibano M, Kusano G, Watanabe H (2010) Aim for Production of Glycyrrhiza Radix in Japan (2) Selection of Pharmaceutically Fine Strains from Glycyrrhiza uralensis Fisher. Japanese Soc Pharmacogn 64:76\u201382","journal-title":"Japanese Soc Pharmacogn"},{"key":"2072_CR3","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1248\/yakushi.123.619","volume":"123","author":"G Kusano","year":"2003","unstructured":"Kusano G, Shibano M, Watanabe H, Ozaki K (2003) Pharmaceutical botanical studies on some Glycyrrhiza species. Yakugaku Zasshi 123:619\u2013631. https:\/\/doi.org\/10.1248\/yakushi.123.619","journal-title":"Yakugaku Zasshi"},{"key":"2072_CR4","unstructured":"The Society of Japanese Pharmacopoeia (2021) The Japanese Pharmacopoeia Eighteenth Edition"},{"key":"2072_CR5","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1093\/pcp\/pcad161","volume":"65","author":"N Chiyo","year":"2024","unstructured":"Chiyo N, Seki H, Kanamoto T, Ueda H, Kojoma M, Muranaka T (2024) Glycyrrhizin production in licorice hairy roots based on metabolic redirection of triterpenoid biosynthetic pathway by genome editing. Plant Cell Physiol 65:185\u2013198. https:\/\/doi.org\/10.1093\/pcp\/pcad161","journal-title":"Plant Cell Physiol"},{"key":"2072_CR6","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1186\/s40659-019-0246-3","volume":"52","author":"T Isah","year":"2019","unstructured":"Isah T (2019) Stress and defense responses in plant secondary metabolites production. Biol Res 52:39. https:\/\/doi.org\/10.1186\/s40659-019-0246-3","journal-title":"Biol Res"},{"key":"2072_CR7","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2020.00007","volume":"11","author":"MK Davies","year":"2020","unstructured":"Davies MK, Jibran R, Zhou Y, Albert WN, Brummell AD, Jordan RB, Bowman LJ, Schwinn EK (2020) The evolution of flavonoid biosynthesis: a bryophyte perspective. Front Plant Sci 11:7. https:\/\/doi.org\/10.3389\/fpls.2020.00007","journal-title":"Front Plant Sci"},{"key":"2072_CR8","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1111\/tps.15387","volume":"107","author":"S Vries","year":"2021","unstructured":"Vries S, F\u00fcrst-Jansen RMJ, Irisarri I, Ashok DA, Ischebeck T, Feussner K, Abreu NI, Petersen M, Feussner I, Vries J (2021) The evolution of the phenylpropanoid pathway entailed pronounced radiations and divergences of enzyme families. Plant J 107:975\u20131002. https:\/\/doi.org\/10.1111\/tps.15387","journal-title":"Plant J"},{"key":"2072_CR9","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.796045","volume":"12","author":"PJ Hawkins","year":"2022","unstructured":"Hawkins PJ, Oresnik IJ (2022) The Rhizobium-legume symbiosis: co-opting successful stress management. Front Plant Sci 12:796045. https:\/\/doi.org\/10.3389\/fpls.2021.796045","journal-title":"Front Plant Sci"},{"key":"2072_CR10","doi-asserted-by":"publisher","first-page":"57","DOI":"10.5511\/plantbiotechnology.20.1124a","volume":"38","author":"I Kusaba","year":"2021","unstructured":"Kusaba I, Nakao T, Maita H, Sato S, Chijiiwa R, Yamada E, Arima S, Mareshige K, Ishimaru K, Akashi R, Suzuki A (2021) Mesorhizobium sp. J8 can establish symbiosis with Glycyrrhiza uralensis, increasing glycyrrhizin production. Plant Biotechnol 38:57\u201366. https:\/\/doi.org\/10.5511\/plantbiotechnology.20.1124a","journal-title":"Plant Biotechnol"},{"key":"2072_CR11","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1007\/s11418-025-01918-2","volume":"79","author":"S Yamamoto","year":"2025","unstructured":"Yamamoto S, Shimomura A, Watanabe S, Kojoma M, Suzuki A (2025) Overwhelming glycyrrhizin production in Glycyrrhiza glabra induced by rhizobial symbiosis. J Nat Med 79:833\u2013844. https:\/\/doi.org\/10.1007\/s11418-025-01918-2","journal-title":"J Nat Med"},{"key":"2072_CR12","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-023-42877-0","volume":"14","author":"X Chen","year":"2023","unstructured":"Chen X, Hudson GA, Mineo C, Amer B, Baidoo EEK, Crowe SA, Liu Y, Keasling JD, Scheller HV (2023) Deciphering triterpenoid saponin biosynthesis by leveraging transcriptome response to methyl jasmonate elicitation in Saponaria vaccaria. Nat Commun 14:1\u201315. https:\/\/doi.org\/10.1038\/s41467-023-42877-0","journal-title":"Nat Commun"},{"key":"2072_CR13","doi-asserted-by":"publisher","first-page":"995","DOI":"10.1111\/j.1467-7652.2012.00729.x","volume":"10","author":"S Hayashi","year":"2012","unstructured":"Hayashi S, Reid DE, Lorenc MT, Stiller J, Edwards D, Gresshoff PM, Ferguson BJ (2012) Transient Nod factor-dependent gene expression in the nodulation-competent zone of soybean (Glycine max [L.] Merr.) roots. Plant Biotechnol J 10:995\u20131010. https:\/\/doi.org\/10.1111\/j.1467-7652.2012.00729.x","journal-title":"Plant Biotechnol J"},{"key":"2072_CR14","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1111\/tpj.13385","volume":"89","author":"K Mochida","year":"2017","unstructured":"Mochida K, Sakurai T, Seki H, Yoshida T, Takahagi K, Sawai S, Uchiyama H, Muranaka T, Saito K (2017) Draft genome assembly and annotation of Glycyrrhiza uralensis, a medicinal legume. Plant J 89:181\u2013194. https:\/\/doi.org\/10.1111\/tpj.13385","journal-title":"Plant J"},{"key":"2072_CR15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1093\/dnares\/dsac043","volume":"29","author":"R Amit","year":"2022","unstructured":"Amit R, Hirakawa H, Megha R, Shimizu Y, Shirasawa K, Kikuchi S, Seki H, Yamazaki M, Toyoda A, Isobe S, Muranaka T, Saito K (2022) Chromosome-scale genome assembly of Glycyrrhiza uralensis revealed metabolic gene cluster centred specialized metabolites biosynthesis. DNA Res 29:1\u201314. https:\/\/doi.org\/10.1093\/dnares\/dsac043","journal-title":"DNA Res"},{"key":"2072_CR16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41467-026-68881-8","volume":"17","author":"Z Zhang","year":"2026","unstructured":"Zhang Z, Li W, Meng F, Jin Y, Sun W, Kuang Q, Ren S, Liu W, Zhang L, Qin L, Lv B, Jia H, Li C (2026) Discover the maze-like network for glabridin biosynthesis. Nat Commun 17:1\u201314. https:\/\/doi.org\/10.1038\/s41467-026-68881-8","journal-title":"Nat Commun"},{"key":"2072_CR17","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1042\/bj1251075","volume":"125","author":"WJ Broughton","year":"1971","unstructured":"Broughton WJ, Dilworth MJ (1971) Control of leghemoglobin synthesis in snake beans. Biochem J 125:1075\u20131080. https:\/\/doi.org\/10.1042\/bj1251075","journal-title":"Biochem J"},{"key":"2072_CR18","doi-asserted-by":"publisher","first-page":"1955","DOI":"10.1093\/bioinformatics\/btz854","volume":"36","author":"C Prieto","year":"2020","unstructured":"Prieto C, Barrios D (2020) RaNA-Seq: interactive RNA-Seq analysis from FASTQ files to functional analysis. Bioinformatics 36:1955\u20131956. https:\/\/doi.org\/10.1093\/bioinformatics\/btz854","journal-title":"Bioinformatics"},{"key":"2072_CR19","doi-asserted-by":"publisher","first-page":"772","DOI":"10.1093\/molbev\/mst010","volume":"30","author":"K Katoh","year":"2013","unstructured":"Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol 30:772\u2013780. https:\/\/doi.org\/10.1093\/molbev\/mst010","journal-title":"Mol Biol Evol"},{"key":"2072_CR20","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msag117","volume":"43","author":"TKF Wong","year":"2026","unstructured":"Wong TKF, Ly-Trong N, Ren H, Demotte P, Ba\u00f1os H, Roger AJ, Susko E, Bielow C, De Maio N, Goldman N, Hahn MW, Dos Reis M, Vinh LS, Huttley G, Lanfear R, Minh BQ (2026) IQ-TREE 3: phylogenomic inference software using complex evolutionary models. Mol Biol Evol 43:msag117. https:\/\/doi.org\/10.1093\/molbev\/msag117","journal-title":"Mol Biol Evol"},{"key":"2072_CR21","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1093\/bioinformatics\/btp033","volume":"25","author":"AM Waterhouse","year":"2009","unstructured":"Waterhouse AM, Procter JB, Martin DMA, Clamp M, Barton GJ (2009) Jalview Version 2\u2014a multiple sequence alignment editor and analysis workbench. Bioinformatics 25:1189\u20131191. https:\/\/doi.org\/10.1093\/bioinformatics\/btp033","journal-title":"Bioinformatics"},{"key":"2072_CR22","doi-asserted-by":"publisher","first-page":"D384","DOI":"10.1093\/nar\/gkac1096","volume":"51","author":"J Wang","year":"2023","unstructured":"Wang J, Chitsaz F, Derbyshire MK, Gonzales NR, Gwadz M, Lu S, Marchler GH, Song JS, Thanki N, Yamashita RA, Yang M, Zhang D, Zheng C, Lanczycki CJ, Marchler-Bauer A (2023) The conserved domain database in 2023. Nucleic Acids Res 51:D384\u2013D388. https:\/\/doi.org\/10.1093\/nar\/gkac1096","journal-title":"Nucleic Acids Res"},{"key":"2072_CR23","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","volume":"25","author":"KJ Livak","year":"2001","unstructured":"Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2009\u2013 \u2206\u2206CT method. Methods 25:402\u2013408. https:\/\/doi.org\/10.1006\/meth.2001.1262","journal-title":"Methods"},{"key":"2072_CR24","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-025-92115-4","volume":"15","author":"J Kim","year":"2025","unstructured":"Kim J, Lee J, Kang JS, Shim H, Kang D, Lee SH, Choi JP, Kim HS, Kim MS, Kim YI, Lee Y, Ziyaev Z, Shin YK, Bhak J, Yang TJ (2025) Contributions of interspecific hybrids to genetic variability in Glycyrrhiza uralensis and G. glabra. Sci Rep 15:1\u201313. https:\/\/doi.org\/10.1038\/s41598-025-92115-4","journal-title":"Sci Rep"},{"key":"2072_CR25","doi-asserted-by":"publisher","first-page":"788","DOI":"10.1038\/nature08854","volume":"464","author":"L Pauwels","year":"2010","unstructured":"Pauwels L, Barbero GF, Geerinck J, Tilleman S, Grunewald W, Perez AC, Chico JM, Bossche RV, Sewell J, Gil E, Garc\u00eda-Casado G, Witters E, Inz\u00e9 D, Long J, Jaeger G, Solano R, Goossens A (2010) NINJA connects the co-repressor TOPLESS to jasmonate signalling. Nature 464:788\u2013791. https:\/\/doi.org\/10.1038\/nature08854","journal-title":"Nature"},{"key":"2072_CR26","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.920172","volume":"13","author":"H Yao","year":"2022","unstructured":"Yao H, Wang F, Bi Q, Liu H, Liu L, Xiao G, Zhu J, Shen H, Li H (2022) Combined analysis of pharmaceutical active ingredients and transcriptomes of Glycyrrhiza uralensis under PEG6000-induced drought stress revealed glycyrrhizic acid and flavonoids accumulation via JA-mediated signaling. Front Plant Sci 13:920172. https:\/\/doi.org\/10.3389\/fpls.2022.920172","journal-title":"Front Plant Sci"},{"key":"2072_CR27","doi-asserted-by":"publisher","first-page":"1806","DOI":"10.1073\/pnas.1913349117","volume":"117","author":"Y Shimoda","year":"2020","unstructured":"Shimoda Y, Nishigaya Y, Yamaya-Ito H, Inagaki N, Umehara Y, Hirakawa H, Sato S, Yamazaki T, Hayashi M (2020) The rhizobial autotransporter determines the symbiotic nitrogen fixation activity of Lotus japonicus in a host-specific manner. Proc Natl Acad Sci U S A 117:1806\u20131815. https:\/\/doi.org\/10.1073\/pnas.1913349117","journal-title":"Proc Natl Acad Sci U S A"},{"key":"2072_CR28","doi-asserted-by":"publisher","DOI":"10.1038\/srep39447","volume":"6","author":"T Mun","year":"2016","unstructured":"Mun T, Bachmann A, Gupta V, Stougaard J, Andersen SU (2016) Lotus Base: an integrated information portal for the model legume Lotus japonicus. Sci Rep 6:39447. https:\/\/doi.org\/10.1038\/srep39447","journal-title":"Sci Rep"}],"container-title":["Journal of Natural Medicines"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11418-026-02072-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11418-026-02072-z","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11418-026-02072-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T15:33:00Z","timestamp":1793460780000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11418-026-02072-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,15]]},"references-count":28,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["2072"],"URL":"https:\/\/doi.org\/10.1007\/s11418-026-02072-z","relation":{},"ISSN":["1340-3443","1861-0293"],"issn-type":[{"value":"1340-3443","type":"print"},{"value":"1861-0293","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,15]]},"assertion":[{"value":"20 May 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 September 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"All authors have and declare that: (i) no support, financial or otherwise, has been received from any organization that may have an interest in the submitted work; and (ii) there are no other relationships or activities that could appear to have influenced the submitted work.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}}]}}