{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T17:03:01Z","timestamp":1778086981200,"version":"3.51.4"},"reference-count":66,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2019,3,1]],"date-time":"2019-03-01T00:00:00Z","timestamp":1551398400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci. China Life Sci."],"published-print":{"date-parts":[[2019,3]]},"DOI":"10.1007\/s11427-019-9504-5","type":"journal-article","created":{"date-parts":[[2019,3,21]],"date-time":"2019-03-21T09:03:34Z","timestamp":1553159014000},"page":"381-393","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Loss of heterozygosity by SCRaMbLEing"],"prefix":"10.1007","volume":"62","author":[{"given":"Yunxiang","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lu","family":"Ma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhou","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhai","family":"Xiao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingjin","family":"Yuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2019,3,1]]},"reference":[{"key":"9504_CR1","doi-asserted-by":"publisher","first-page":"1179","DOI":"10.1534\/genetics.108.089250","volume":"179","author":"M.P. Andersen","year":"2008","unstructured":"Andersen, M.P., Nelson, Z.W., Hetrick, E.D., and Gottschling, D.E. (2008). A genetic screen for increased loss of heterozygosity in Saccharomyces cerevisiae. Genetics 179, 1179\u20131195.","journal-title":"Genetics"},{"key":"9504_CR2","volume-title":"Cold Spring Harbor Perspect Med","author":"R.J. Bennett","year":"2014","unstructured":"Bennett, R.J., Forche, A., and Berman, J. (2014). Rapid mechanisms for generating genome diversity: whole ploidy shifts, aneuploidy, and loss of heterozygosity. Cold Spring Harbor Perspect Med 4, a019604."},{"key":"9504_CR3","doi-asserted-by":"publisher","first-page":"1932","DOI":"10.1038\/s41467-018-03143-w","volume":"9","author":"B.A. Blount","year":"2018","unstructured":"Blount, B.A., Gowers, G.O.F., Ho, J.C.H., Ledesma-Amaro, R., Jovicevic, D., McKiernan, R.M., Xie, Z.X., Li, B.Z., Yuan, Y.J., and Ellis, T. (2018). Rapid host strain improvement by in vivo rearrangement of a synthetic yeast chromosome. Nat Commun 9, 1932.","journal-title":"Nat Commun"},{"key":"9504_CR4","doi-asserted-by":"publisher","first-page":"756","DOI":"10.1038\/369756a0","volume":"369","author":"E.J. Brown","year":"1994","unstructured":"Brown, E.J., Albers, M.W., Bum Shin, T., Ichikawa, K., Keith, C.T., Lane, W.S., and Schreiber, S.L. (1994). A mammalian protein targeted by G1-arresting rapamycin-receptor complex. Nature 369, 756\u2013758.","journal-title":"Nature"},{"key":"9504_CR5","doi-asserted-by":"publisher","first-page":"3271","DOI":"10.1101\/gad.13.24.3271","volume":"13","author":"M.E. Cardenas","year":"1999","unstructured":"Cardenas, M.E., Cutler, N.S., Lorenz, M.C., Di Como, C.J. and Heitman, J. (1999). The TOR signaling cascade regulates gene expression in response to nutrients. Genes Dev 13, 3271\u20133279.","journal-title":"Genes Dev"},{"key":"9504_CR6","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1038\/nature10795","volume":"482","author":"G. Chen","year":"2012","unstructured":"Chen, G., Bradford, W.D., Seidel, C.W., and Li, R. (2012). Hsp90 stress potentiates rapid cellular adaptation through induction of aneuploidy. Nature 482, 246\u2013250.","journal-title":"Nature"},{"key":"9504_CR7","doi-asserted-by":"publisher","first-page":"778","DOI":"10.1111\/j.0960-7412.2003.01999.x","volume":"37","author":"L. Comai","year":"2004","unstructured":"Comai, L., Young, K., Till, B.J., Reynolds, S.H., Greene, E.A., Codomo, C. A., Enns, L.C., Johnson, J.E., Burtner, C., Odden, A.R., et al. (2004). Efficient discovery of DNA polymorphisms in natural populations by Ecotilling. Plant J 37, 778\u2013786.","journal-title":"Plant J"},{"key":"9504_CR8","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1128\/MMBR.66.4.579-591.2002","volume":"66","author":"J.L. Crespo","year":"2002","unstructured":"Crespo, J.L., and Hall, M.N. (2002). Elucidating TOR signaling and rapamycin action: lessons from Saccharomyces cerevisiae. Microbiol Mol Biol Rev 66, 579\u2013591.","journal-title":"Microbiol Mol Biol Rev"},{"key":"9504_CR9","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1038\/nrg2811","volume":"11","author":"B. Dujon","year":"2010","unstructured":"Dujon, B. (2010). Yeast evolutionary genomics. Nat Rev Genet 11, 512\u2013524.","journal-title":"Nat Rev Genet"},{"key":"9504_CR10","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1038\/nature02579","volume":"430","author":"B. Dujon","year":"2004","unstructured":"Dujon, B., Sherman, D., Fischer, G., Durrens, P., Casaregola, S., Lafontaine, I., De Montigny, J., Marck, C., Neuv\u00e9glise, C., Talla, E., et al. (2004). Genome evolution in yeasts. Nature 430, 35\u201344.","journal-title":"Nature"},{"key":"9504_CR11","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1038\/nature10403","volume":"477","author":"J.S. Dymond","year":"2011","unstructured":"Dymond, J.S., Richardson, S.M., Coombes, C.E., Babatz, T., Muller, H., Annaluru, N., Blake, W.J., Schwerzmann, J.W., Dai, J., Lindstrom, D. L., et al. (2011). Synthetic chromosome arms function in yeast and generate phenotypic diversity by design. Nature 477, 471\u2013476.","journal-title":"Nature"},{"key":"9504_CR12","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/S0076-6879(02)50957-5","volume":"350","author":"R.D. Gietz","year":"2002","unstructured":"Gietz, R.D., and Woods, R.A. (2002). Transformation of yeast by lithium acetate\/single-stranded carrier DNA\/polyethylene glycol method. Methods Enzymol 350, 87\u201396.","journal-title":"Methods Enzymol"},{"key":"9504_CR13","first-page":"1","volume-title":"In eLS.","author":"K.L. Gorringe","year":"2016","unstructured":"Gorringe, K.L. (2016). Loss of heterozygosity. In eLS. pp. 1\u20138, doi: 10.1002\/9780470015902.a0026643."},{"key":"9504_CR14","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1038\/37925","volume":"389","author":"F. Guo","year":"1997","unstructured":"Guo, F., Gopaul, D.N., and van Duyne, G.D. (1997). Structure of Cre recombinase complexed with DNA in a site-specific recombination synapse. Nature 389, 40\u201346.","journal-title":"Nature"},{"key":"9504_CR15","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1002\/wat2.1014","volume":"1","author":"C. Hassall","year":"2014","unstructured":"Hassall, C. (2014). The ecology and biodiversity of urban ponds. WIREs Water 1, 187\u2013206.","journal-title":"WIREs Water"},{"key":"9504_CR16","doi-asserted-by":"publisher","first-page":"6680","DOI":"10.1038\/sj.onc.1204091","volume":"19","author":"M. Hidalgo","year":"2000","unstructured":"Hidalgo, M., and Rowinsky, E.K. (2000). The rapamycin-sensitive signal transduction pathway as a target for cancer therapy. Oncogene 19, 6680\u20136686.","journal-title":"Oncogene"},{"key":"9504_CR17","doi-asserted-by":"publisher","first-page":"1931","DOI":"10.1038\/s41467-017-02208-6","volume":"9","author":"L. Hochrein","year":"2018","unstructured":"Hochrein, L., Mitchell, L.A., Schulz, K., Messerschmidt, K., and Mueller-Roeber, B. (2018). L-SCRaMbLE as a tool for light-controlled Cremediated recombination in yeast. Nat Commun 9, 1931.","journal-title":"Nat Commun"},{"key":"9504_CR18","doi-asserted-by":"publisher","first-page":"1929","DOI":"10.1101\/gad.532109","volume":"23","author":"A. Huber","year":"2009","unstructured":"Huber, A., Bodenmiller, B., Uotila, A., Stahl, M., Wanka, S., Gerrits, B., Aebersold, R., and Loewith, R. (2009). Characterization of the rapamycin-sensitive phosphoproteome reveals that Sch9 is a central coordinator of protein synthesis. Genes Dev 23, 1929\u20131943.","journal-title":"Genes Dev"},{"key":"9504_CR19","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1038\/nbt.4060","volume":"36","author":"M. Jain","year":"2018","unstructured":"Jain, M., Koren, S., Miga, K.H., Quick, J., Rand, A.C., Sasani, T.A., Tyson, J.R., Beggs, A.D., Dilthey, A.T., Fiddes, I.T., et al. (2018). Nanopore sequencing and assembly of a human genome with ultra-long reads. Nat Biotechnol 36, 338\u2013345.","journal-title":"Nat Biotechnol"},{"key":"9504_CR20","doi-asserted-by":"publisher","first-page":"14061","DOI":"10.1038\/ncomms14061","volume":"8","author":"D.C. Jeffares","year":"2017","unstructured":"Jeffares, D.C., Jolly, C., Hoti, M., Speed, D., Shaw, L., Rallis, C., Balloux, F., Dessimoz, C., B\u00e4hler, J., and Sedlazeck, F.J. (2017). Transient structural variations have strong effects on quantitative traits and reproductive isolation in fission yeast. Nat Commun 8, 14061.","journal-title":"Nat Commun"},{"key":"9504_CR21","doi-asserted-by":"publisher","first-page":"1933","DOI":"10.1038\/s41467-018-03084-4","volume":"9","author":"B. Jia","year":"2018","unstructured":"Jia, B., Wu, Y., Li, B.Z., Mitchell, L.A., Liu, H., Pan, S., Wang, J., Zhang, H.R., Jia, N., Li, B., et al. (2018). Precise control of SCRaMbLE in synthetic haploid and diploid yeast. Nat Commun 9, 1933.","journal-title":"Nat Commun"},{"key":"9504_CR22","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1038\/nature06862","volume":"453","author":"J.M. Kidd","year":"2008","unstructured":"Kidd, J.M., Cooper, G.M., Donahue, W.F., Hayden, H.S., Sampas, N., Graves, T., Hansen, N., Teague, B., Alkan, C., Antonacci, F., et al. (2008). Mapping and sequencing of structural variation from eight human genomes. Nature 453, 56\u201364.","journal-title":"Nature"},{"key":"9504_CR23","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1038\/ng1195-241","volume":"11","author":"E. Lander","year":"1995","unstructured":"Lander, E., and Kruglyak, L. (1995). Genetic dissection of complex traits: guidelines for interpreting and reporting linkage results. Nat Genet 11, 241\u2013247.","journal-title":"Nat Genet"},{"key":"9504_CR24","doi-asserted-by":"publisher","first-page":"2037","DOI":"10.1126\/science.8091226","volume":"265","author":"E.S. Lander","year":"1994","unstructured":"Lander, E.S., and Schork, N.J. (1994). Genetic dissection of complex traits. Science 265, 2037\u20132048.","journal-title":"Science"},{"key":"9504_CR25","doi-asserted-by":"publisher","first-page":"602","DOI":"10.1002\/pd.4076","volume":"33","author":"T.K. Lau","year":"2013","unstructured":"Lau, T.K., Jiang, F.M., Stevenson, R.J., Lo, T.K., Chan, L.W., Chan, M.K., Lo, P.S.S., Wang, W., Zhang, H.Y., Chen, F., et al. (2013). Secondary findings from non-invasive prenatal testing for common fetal aneuploidies by whole genome sequencing as a clinical service. Prenat Diagn 33, 602\u2013608.","journal-title":"Prenat Diagn"},{"key":"9504_CR26","doi-asserted-by":"publisher","first-page":"e3000069","DOI":"10.1371\/journal.pbio.3000069","volume":"16","author":"S. Lauer","year":"2018","unstructured":"Lauer, S., Avecilla, G., Spealman, P., Sethia, G., Brandt, N., Levy, S.F., and Gresham, D. (2018). Single-cell copy number variant detection reveals the dynamics and diversity of adaptation. PLoS Biol 16, e3000069.","journal-title":"PLoS Biol"},{"key":"9504_CR27","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1038\/386623a0","volume":"386","author":"C. Lengauer","year":"1997","unstructured":"Lengauer, C., Kinzler, K.W., and Vogelstein, B. (1997). Genetic instability in colorectal cancers. Nature 386, 623\u2013627.","journal-title":"Nature"},{"key":"9504_CR28","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1038\/ng1097-223","volume":"17","author":"M. Lewandoski","year":"1997","unstructured":"Lewandoski, M., and Martin, G.R. (1997). Cre-mediated chromosome loss in mice. Nat Genet 17, 223\u2013225.","journal-title":"Nat Genet"},{"key":"9504_CR29","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1534\/genetics.109.106229","volume":"183","author":"D.L. Lindstrom","year":"2009","unstructured":"Lindstrom, D.L., and Gottschling, D.E. (2009). The Mother Enrichment Program: A genetic system for facile replicative life span analysis in Saccharomyces cerevisiae. Genetics 183, 413\u2013422.","journal-title":"Genetics"},{"key":"9504_CR30","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1038\/nature07743","volume":"458","author":"G. Liti","year":"2009","unstructured":"Liti, G., Carter, D.M., Moses, A.M., Warringer, J., Parts, L., James, S.A., Davey, R.P., Roberts, I.N., Burt, A., Koufopanou, V., et al. (2009). Population genomics of domestic and wild yeasts. Nature 458, 337\u2013341.","journal-title":"Nature"},{"key":"9504_CR31","doi-asserted-by":"publisher","first-page":"1936","DOI":"10.1038\/s41467-018-04254-0","volume":"9","author":"W. Liu","year":"2018","unstructured":"Liu, W., Luo, Z., Wang, Y., Pham, N.T., Tuck, L., P\u00e9rez-Pi, I., Liu, L., Shen, Y., French, C., Auer, M., et al. (2018). Rapid pathway prototyping and engineering using in vitro and in vivo synthetic genome SCRaMbLE-in methods. Nat Commun 9, 1936.","journal-title":"Nat Commun"},{"key":"9504_CR32","doi-asserted-by":"publisher","first-page":"1627","DOI":"10.1126\/science.1229112","volume":"338","author":"S. Lu","year":"2012","unstructured":"Lu, S., Zong, C., Fan, W., Yang, M., Li, J., Chapman, A.R., Zhu, P., Hu, X., Xu, L., Yan, L., et al. (2012). Probing meiotic recombination and aneuploidy of single sperm cells by whole-genome sequencing. Science 338, 1627\u20131630.","journal-title":"Science"},{"key":"9504_CR33","doi-asserted-by":"publisher","first-page":"1930","DOI":"10.1038\/s41467-017-00806-y","volume":"9","author":"Z. Luo","year":"2018","unstructured":"Luo, Z., Wang, L., Wang, Y., Zhang, W., Guo, Y., Shen, Y., Jiang, L., Wu, Q., Zhang, C., Cai, Y., et al. (2018a). Identifying and characterizing SCRaMbLEd synthetic yeast using ReSCuES. Nat Commun 9, 1930.","journal-title":"Nat Commun"},{"key":"9504_CR34","doi-asserted-by":"publisher","first-page":"1515","DOI":"10.1007\/s11427-018-9403-y","volume":"61","author":"Z. Luo","year":"2018","unstructured":"Luo, Z., Yang, Q., Geng, B., Jiang, S., Yang, S., Li, X., Cai, Y., and Dai, J. (2018b). Whole genome engineering by synthesis. Sci China Life Sci 61, 1515\u20131527.","journal-title":"Sci China Life Sci"},{"key":"9504_CR35","volume-title":"Science","author":"G. Mercy","year":"2017","unstructured":"Mercy, G., Mozziconacci, J., Scolari, V.F., Yang, K., Zhao, G., Thierry, A., Luo, Y., Mitchell, L.A., Shen, M., Shen, Y., et al. (2017). 3D organization of synthetic and scrambled chromosomes. Science 355, eaaf4597."},{"key":"9504_CR36","volume-title":"Science","author":"L.A. Mitchell","year":"2017","unstructured":"Mitchell, L.A., Wang, A., Stracquadanio, G., Kuang, Z., Wang, X., Yang, K., Richardson, S., Martin, J.A., Zhao, Y., Walker, R., et al. (2017). Synthesis, debugging, and effects of synthetic chromosome consolidation: synVI and beyond. Science 355, eaaf4831."},{"key":"9504_CR37","first-page":"3552","volume":"21","author":"A.F. O\u2019Donnell","year":"2010","unstructured":"O\u2019Donnell, A.F., Apffel, A., Gardner, R.G., and Cyert, M.S. (2010). \u03b1-Arrestins Aly1 and Aly2 regulate intracellular trafficking in response to nutrient signaling. Mol Biol Cell 21, 3552\u20133566.","journal-title":"\u03b1-Arrestins Aly1 and Aly2 regulate intracellular trafficking in response to nutrient signaling. Mol Biol Cell"},{"key":"9504_CR38","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1038\/s41586-018-0030-5","volume":"556","author":"J. Peter","year":"2018","unstructured":"Peter, J., De Chiara, M., Friedrich, A., Yue, J.X., Pflieger, D., Bergstr\u00f6m, A., Sigwalt, A., Barre, B., Freel, K., Llored, A., et al. (2018). Genome evolution across 1,011 Saccharomyces cerevisiae isolates. Nature 556, 339\u2013344.","journal-title":"Nature"},{"key":"9504_CR39","doi-asserted-by":"publisher","first-page":"e140","DOI":"10.1093\/nar\/gng140","volume":"31","author":"O. Philipp","year":"2003","unstructured":"Philipp, O., Otipoby, K.L., Mitsuo, M., and Klaus, R. (2003). Unidirectional Cre-mediated genetic inversion in mice using the mutant loxP pair lox66\/lox71. Nucleic Acids Res 31, e140.","journal-title":"Nucleic Acids Res"},{"key":"9504_CR40","first-page":"181","volume":"21","author":"C.G. Proud","year":"1996","unstructured":"Proud, C.G. (1996). p70 S6 kinase: an enigma with variations. Trends Biochem Sci 21, 181\u2013185.","journal-title":"p70 S6 kinase: an enigma with variations. Trends Biochem Sci"},{"key":"9504_CR41","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1016\/S1369-5266(02)00240-6","volume":"5","author":"A. Rafalski","year":"2002","unstructured":"Rafalski, A. (2002). Applications of single nucleotide polymorphisms in crop genetics. Curr Opin Plant Biol 5, 94\u2013100.","journal-title":"Curr Opin Plant Biol"},{"key":"9504_CR42","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1038\/nature05329","volume":"444","author":"R. Redon","year":"2006","unstructured":"Redon, R., Ishikawa, S., Fitch, K.R., Feuk, L., Perry, G.H., Andrews, T.D., Fiegler, H., Shapero, M.H., Carson, A.R., Chen, W., et al. (2006). Global variation in copy number in the human genome. Nature 444, 444\u2013454.","journal-title":"Nature"},{"key":"9504_CR43","doi-asserted-by":"publisher","first-page":"1799","DOI":"10.1534\/genetics.108.087999","volume":"180","author":"R.J. Reid","year":"2008","unstructured":"Reid, R.J., Sunjevaric, I., Voth, W.P., Ciccone, S., Du, W., Olsen, A.E., Stillman, D.J., and Rothstein, R. (2008). Chromosome-scale genetic mapping using a set of 16 conditionally stable Saccharomyces cerevisiae chromosomes. Genetics 180, 1799\u20131808.","journal-title":"Genetics"},{"key":"9504_CR44","doi-asserted-by":"publisher","first-page":"558","DOI":"10.1007\/s11427-017-9039-6","volume":"60","author":"H. Ren","year":"2017","unstructured":"Ren, H., and Zhao, H. (2017). SynV and SynX: A story more than DNA synthesis. Sci China Life Sci 60, 558\u2013560.","journal-title":"Sci China Life Sci"},{"key":"9504_CR45","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.1126\/science.aaf4557","volume":"355","author":"S.M. Richardson","year":"2017","unstructured":"Richardson, S.M., Mitchell, L.A., Stracquadanio, G., Yang, K., Dymond, J. S., DiCarlo, J.E., Lee, D., Huang, C.L.V., Chandrasegaran, S., Cai, Y., et al. (2017). Design of a synthetic yeast genome. Science 355, 1040\u20131044.","journal-title":"Science"},{"key":"9504_CR46","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1038\/nrc1974","volume":"6","author":"D.M. Sabatini","year":"2006","unstructured":"Sabatini, D.M. (2006). mTOR and cancer: insights into a complex relationship. Nat Rev Cancer 6, 729\u2013734.","journal-title":"Nat Rev Cancer"},{"key":"9504_CR47","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1038\/nprot.2008.73","volume":"3","author":"T.D. Schmittgen","year":"2008","unstructured":"Schmittgen, T.D., and Livak, K.J. (2008). Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3, 1101\u20131108.","journal-title":"Nat Protoc"},{"key":"9504_CR48","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/0092-8674(84)90301-5","volume":"37","author":"D.C. Schwartz","year":"1984","unstructured":"Schwartz, D.C., and Cantor, C.R. (1984). Separation of yeast chromosomesized DNAs by pulsed field gradient gel electrophoresis. Cell 37, 67\u201375.","journal-title":"Cell"},{"key":"9504_CR49","doi-asserted-by":"publisher","first-page":"1934","DOI":"10.1038\/s41467-018-04157-0","volume":"9","author":"M.J. Shen","year":"2018","unstructured":"Shen, M.J., Wu, Y., Yang, K., Li, Y., Xu, H., Zhang, H., Li, B.Z., Li, X., Xiao, W.H., Zhou, X., et al. (2018). Heterozygous diploid and interspecies SCRaMbLEing. Nat Commun 9, 1934.","journal-title":"Nat Commun"},{"key":"9504_CR50","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1101\/gr.193433.115","volume":"26","author":"Y. Shen","year":"2016","unstructured":"Shen, Y., Stracquadanio, G., Wang, Y., Yang, K., Mitchell, L.A., Xue, Y., Cai, Y., Chen, T., Dymond, J.S., Kang, K., et al. (2016). SCRaMbLE generates designed combinatorial stochastic diversity in synthetic chromosomes. Genome Res 26, 36\u201349.","journal-title":"Genome Res"},{"key":"9504_CR51","volume-title":"Science","author":"Y. Shen","year":"2017","unstructured":"Shen, Y., Wang, Y., Chen, T., Gao, F., Gong, J., Abramczyk, D., Walker, R., Zhao, H., Chen, S., Liu, W., et al. (2017). Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome. Science 355, eaaf4791."},{"key":"9504_CR52","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1146\/annurev-med-100708-204735","volume":"61","author":"P. Stankiewicz","year":"2010","unstructured":"Stankiewicz, P., and Lupski, J.R. (2010). Structural variation in the human genome and its role in disease. Annu Rev Med 61, 437\u2013455.","journal-title":"Annu Rev Med"},{"key":"9504_CR53","doi-asserted-by":"publisher","first-page":"916","DOI":"10.1126\/science.1142210","volume":"317","author":"E.M. Torres","year":"2007","unstructured":"Torres, E.M., Sokolsky, T., Tucker, C.M., Chan, L.Y., Boselli, M., Dunham, M.J., and Amon, A. (2007). Effects of aneuploidy on cellular physiology and cell division in haploid yeast. Science 317, 916\u2013924.","journal-title":"Science"},{"key":"9504_CR54","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1186\/1471-2156-9-8","volume":"9","author":"U. V\u00e4li","year":"2008","unstructured":"V\u00e4li, U., Brandstr\u00f6m, M., Johansson, M., and Ellegren, H. (2008). Insertion-deletion polymorphisms (indels) as genetic markers in natural populations. BMC Genet 9, 8.","journal-title":"BMC Genet"},{"key":"9504_CR55","doi-asserted-by":"publisher","first-page":"3783","DOI":"10.1038\/s41467-018-06216-y","volume":"9","author":"J. Wang","year":"2018","unstructured":"Wang, J., Xie, Z.X., Ma, Y., Chen, X.R., Huang, Y.Q., He, B., Bin Jia, B., Li, B.Z., and Yuan, Y.J. (2018). Ring synthetic chromosome V SCRaMbLE. Nat Commun 9, 3783.","journal-title":"Nat Commun"},{"key":"9504_CR56","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1016\/j.ceb.2006.10.002","volume":"18","author":"B.A.A. Weaver","year":"2006","unstructured":"Weaver, B.A.A., and Cleveland, D.W. (2006). Does aneuploidy cause cancer? Curr Opin Cell Biol 18, 658\u2013667.","journal-title":"Curr Opin Cell Biol"},{"key":"9504_CR57","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12918-016-0290-3","volume":"10","author":"T.S. Weber","year":"2016","unstructured":"Weber, T.S., Dukes, M., Miles, D.C., Glaser, S.P., Naik, S.H., and Duffy, K. R. (2016). Site-specific recombinatorics: in situ cellular barcoding with the Cre Lox system. BMC Syst Biol 10, 1\u201313.","journal-title":"BMC Syst Biol"},{"key":"9504_CR58","volume-title":"Science","author":"Y. Wu","year":"2017","unstructured":"Wu, Y., Li, B.Z., Zhao, M., Mitchell, L.A., Xie, Z.X., Lin, Q.H., Wang, X., Xiao, W.H., Wang, Y., and Zhou, X. (2017). Bug mapping and fitness testing of chemically synthesized chromosome X. Science 355."},{"key":"9504_CR59","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03743-6","volume-title":"In vitro DNA SCRaMbLE","author":"Y. Wu","year":"2018","unstructured":"Wu, Y., Zhu, R.Y., Mitchell, L.A., Ma, L., Liu, R., Zhao, M., Jia, B., Xu, H., Li, Y.X., and Yang, Z.M. (2018). In vitro DNA SCRaMbLE. Nat Commun 9."},{"key":"9504_CR60","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1016\/j.cell.2006.01.016","volume":"124","author":"S. Wullschleger","year":"2006","unstructured":"Wullschleger, S., Loewith, R., and Hall, M.N. (2006). TOR signaling in growth and metabolism. Cell 124, 471\u2013484.","journal-title":"Cell"},{"key":"9504_CR61","volume-title":"Science","author":"Z.X. Xie","year":"2017","unstructured":"Xie, Z.X., Li, B.Z., Mitchell, L.A., Wu, Y., Qi, X., Jin, Z., Jia, B., Wang, X., Zeng, B.X., and Liu, H.M. (2017). \u201cPerfect\u201d designer chromosome V and behavior of a ring derivative. Science 355."},{"key":"9504_CR62","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.mrgentox.2007.10.013","volume":"651","author":"Y. Shen","year":"2008","unstructured":"Shen, Y., Betzendahl, I., Tinneberg, H.R., and Eichenlaub-Ritter, U. (2008). Enhanced polarizing microscopy as a new tool in aneuploidy research in oocytes. Mutat Res\/Genet Toxicol Environ Mutagenesis 651, 131\u2013140.","journal-title":"Mutat Res\/Genet Toxicol Environ Mutagenesis"},{"key":"9504_CR63","doi-asserted-by":"publisher","first-page":"21010","DOI":"10.1073\/pnas.1211150109","volume":"109","author":"A.H. Yona","year":"2012","unstructured":"Yona, A.H., Manor, Y.S., Herbst, R.H., Romano, G.H., Mitchell, A., Kupiec, M., Pilpel, Y., and Dahan, O. (2012). Chromosomal duplication is a transient evolutionary solution to stress. Proc Natl Acad Sci USA 109, 21010\u201321015.","journal-title":"Proc Natl Acad Sci USA"},{"key":"9504_CR64","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1038\/ng.3847","volume":"49","author":"J.X. Yue","year":"2017","unstructured":"Yue, J.X., Li, J., Aigrain, L., Hallin, J., Persson, K., Oliver, K., Bergstr\u00f6m, A., Coupland, P., Warringer, J., Lagomarsino, M.C., et al. (2017). Contrasting evolutionary genome dynamics between domesticated and wild yeasts. Nat Genet 49, 913\u2013924.","journal-title":"Nat Genet"},{"key":"9504_CR65","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1007\/s11427-018-9325-1","volume":"61","author":"F. Zhang","year":"2018","unstructured":"Zhang, F., and Voytas, D.F. (2018). Synthetic genomes engineered by SCRaMbLEing. Sci China Life Sci 61, 975\u2013977.","journal-title":"Sci China Life Sci"},{"key":"9504_CR66","volume-title":"Science","author":"W. Zhang","year":"2017","unstructured":"Zhang, W., Zhao, G., Luo, Z., Lin, Y., Wang, L., Guo, Y., Wang, A., Jiang, S., Jiang, Q., Gong, J., et al. (2017). Engineering the ribosomal DNA in a megabase synthetic chromosome. Science 355, eaaf3981."}],"container-title":["Science China Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11427-019-9504-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/article\/10.1007\/s11427-019-9504-5\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/link.springer.com\/content\/pdf\/10.1007\/s11427-019-9504-5.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,29]],"date-time":"2020-02-29T00:10:29Z","timestamp":1582935029000},"score":1,"resource":{"primary":{"URL":"http:\/\/link.springer.com\/10.1007\/s11427-019-9504-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3]]},"references-count":66,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,3]]}},"alternative-id":["9504"],"URL":"https:\/\/doi.org\/10.1007\/s11427-019-9504-5","relation":{},"ISSN":["1674-7305","1869-1889"],"issn-type":[{"value":"1674-7305","type":"print"},{"value":"1869-1889","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3]]},"assertion":[{"value":"15 January 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"19 February 2019","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 March 2019","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}