{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T14:40:36Z","timestamp":1782225636872,"version":"3.54.5"},"reference-count":44,"publisher":"Springer Science and Business Media LLC","issue":"11","license":[{"start":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T00:00:00Z","timestamp":1652832000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,5,18]],"date-time":"2022-05-18T00:00:00Z","timestamp":1652832000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci. China Life Sci."],"published-print":{"date-parts":[[2022,11]]},"DOI":"10.1007\/s11427-021-2088-1","type":"journal-article","created":{"date-parts":[[2022,5,21]],"date-time":"2022-05-21T12:02:40Z","timestamp":1653134560000},"page":"2269-2286","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Double knock-in pig models with elements of binary Tet-On and phiC31 integrase systems for controllable and switchable gene expression"],"prefix":"10.1007","volume":"65","author":[{"given":"Qin","family":"Jin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shixue","family":"Gou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenpeng","family":"Zhuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanhui","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiayuan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quanjun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaoming","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangbing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weikai","family":"Ge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingke","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengdan","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaozhu","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaowei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Lian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longquan","family":"Quan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinghua","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangxue","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kepin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,5,18]]},"reference":[{"key":"2088_CR1","doi-asserted-by":"publisher","first-page":"1473","DOI":"10.1093\/bioinformatics\/btu048","volume":"30","author":"S Bae","year":"2014","unstructured":"Bae, S., Park, J., and Kim, J.S. (2014). Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases. Bioinformatics 30, 1473\u20131475.","journal-title":"Bioinformatics"},{"key":"2088_CR2","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1002\/gene.20180","volume":"44","author":"C Beard","year":"2006","unstructured":"Beard, C., Hochedlinger, K., Plath, K., Wutz, A., and Jaenisch, R. (2006). Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Genesis 44, 23\u201328.","journal-title":"Genesis"},{"key":"2088_CR3","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1038\/nbt787","volume":"21","author":"G Belteki","year":"2003","unstructured":"Belteki, G., Gertsenstein, M., Ow, D.W., and Nagy, A. (2003). Site-specific cassette exchange and germline transmission with mouse ES cells expressing \u03c6C31 integrase. Nat Biotechnol 21, 321\u2013324.","journal-title":"Nat Biotechnol"},{"key":"2088_CR4","doi-asserted-by":"publisher","first-page":"1616","DOI":"10.1002\/1878-0261.12136","volume":"11","author":"MM Callesen","year":"2017","unstructured":"Callesen, M.M., \u00c1rnad\u00f3ttir, S.S., Lyskjaer, I., \u00d8rntoft, M.B.W., H\u00f8yer, S., Dagnaes-Hansen, F., Liu, Y., Li, R., Callesen, H., Rasmussen, M.H., et al. (2017). A genetically inducible porcine model of intestinal cancer. Mol Oncol 11, 1616\u20131629.","journal-title":"Mol Oncol"},{"key":"2088_CR5","doi-asserted-by":"publisher","first-page":"e19986","DOI":"10.1371\/journal.pone.0019986","volume":"6","author":"W Deng","year":"2011","unstructured":"Deng, W., Yang, D., Zhao, B., Ouyang, Z., Song, J., Fan, N., Liu, Z., Zhao, Y., Wu, Q., Nashun, B., et al. (2011). Use of the 2A peptide for generation of multi-transgenic pigs through a single round of nuclear transfer. PLoS ONE 6, e19986.","journal-title":"PLoS ONE"},{"key":"2088_CR6","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1016\/j.theriogenology.2016.04.058","volume":"86","author":"T Flisikowska","year":"2016","unstructured":"Flisikowska, T., Kind, A., and Schnieke, A. (2016). Pigs as models of human cancers. Theriogenology 86, 433\u2013437.","journal-title":"Theriogenology"},{"key":"2088_CR7","doi-asserted-by":"publisher","first-page":"2196","DOI":"10.1093\/carcin\/bgi190","volume":"26","author":"HS Floyd","year":"2005","unstructured":"Floyd, H.S., Farnsworth, C.L., Kock, N.D., Mizesko, M.C., Little, J.L., Dance, S.T., Everitt, J., Tichelaar, J., Whitsett, J.A., and Miller, M.S. (2005). Conditional expression of the mutant Ki-rasG12C allele results in formation of benign lung adenomas: development of a novel mouse lung tumor model. Carcinogenesis 26, 2196\u20132206.","journal-title":"Carcinogenesis"},{"key":"2088_CR8","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.1101\/gad.5.9.1513","volume":"5","author":"G Friedrich","year":"1991","unstructured":"Friedrich, G., and Soriano, P. (1991). Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev 5, 1513\u20131523.","journal-title":"Genes Dev"},{"key":"2088_CR9","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1038\/s41556-019-0333-2","volume":"21","author":"X Gao","year":"2019","unstructured":"Gao, X., Nowak-Imialek, M., Chen, X., Chen, D., Herrmann, D., Ruan, D., Chen, A.C.H., Eckersley-Maslin, M.A., Ahmad, S., Lee, Y.L., et al. (2019). Establishment of porcine and human expanded potential stem cells. Nat Cell Biol 21, 687\u2013699.","journal-title":"Nat Cell Biol"},{"key":"2088_CR10","doi-asserted-by":"publisher","first-page":"1766","DOI":"10.1126\/science.7792603","volume":"268","author":"M Gossen","year":"1995","unstructured":"Gossen, M., Freundlieb, S., Bender, G., M\u00fcller, G., Hillen, W., and Bujard, H. (1995). Transcriptional activation by tetracyclines in mammalian cells. Science 268, 1766\u20131769.","journal-title":"Science"},{"key":"2088_CR11","doi-asserted-by":"publisher","first-page":"5995","DOI":"10.1073\/pnas.090527097","volume":"97","author":"AC Groth","year":"2000","unstructured":"Groth, A.C., Olivares, E.C., Thyagarajan, B., and Calos, M.P. (2000). A phage integrase directs efficient site-specific integration in human cells. Proc Natl Acad Sci USA 97, 5995\u20136000.","journal-title":"Proc Natl Acad Sci USA"},{"key":"2088_CR12","doi-asserted-by":"publisher","first-page":"695","DOI":"10.1016\/j.neuron.2010.09.027","volume":"68","author":"S Hippenmeyer","year":"2010","unstructured":"Hippenmeyer, S., Youn, Y.H., Moon, H.M., Miyamichi, K., Zong, H., Wynshaw-Boris, A., and Luo, L. (2010). Genetic mosaic dissection of Lis1 and Ndel1 in neuronal migration. Neuron 68, 695\u2013709.","journal-title":"Neuron"},{"key":"2088_CR13","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1038\/nrclinonc.2011.34","volume":"8","author":"L Hutchinson","year":"2011","unstructured":"Hutchinson, L., and Kirk, R. (2011). High drug attrition rates\u2014where are we going wrong? Nat Rev Clin Oncol 8, 189\u2013190.","journal-title":"Nat Rev Clin Oncol"},{"key":"2088_CR14","doi-asserted-by":"publisher","first-page":"1477","DOI":"10.1038\/nbt1362","volume":"25","author":"S Irion","year":"2007","unstructured":"Irion, S., Luche, H., Gadue, P., Fehling, H.J., Kennedy, M., and Keller, G. (2007). Identification and targeting of the ROSA26 locus in human embryonic stem cells. Nat Biotechnol 25, 1477\u20131482.","journal-title":"Nat Biotechnol"},{"key":"2088_CR15","doi-asserted-by":"publisher","first-page":"3243","DOI":"10.1101\/gad.943001","volume":"15","author":"EL Jackson","year":"2001","unstructured":"Jackson, E.L., Willis, N., Mercer, K., Bronson, R.T., Crowley, D., Montoya, R., Jacks, T., and Tuveson, D.A. (2001). Analysis of lung tumor initiation and progression using conditional expression of oncogenic K-ras. Genes Dev 15, 3243\u20133248.","journal-title":"Genes Dev"},{"key":"2088_CR16","doi-asserted-by":"publisher","first-page":"e86146","DOI":"10.1371\/journal.pone.0086146","volume":"9","author":"YX Jin","year":"2014","unstructured":"Jin, Y.X., Jeon, Y., Lee, S.H., Kwon, M.S., Kim, T., Cui, X.S., Hyun, S.H., and Kim, N.H. (2014). Production of pigs expressing a transgene under the control of a tetracycline-inducible system. PLoS ONE 9, e86146.","journal-title":"PLoS ONE"},{"key":"2088_CR17","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.1038\/35074129","volume":"410","author":"L Johnson","year":"2001","unstructured":"Johnson, L., Mercer, K., Greenbaum, D., Bronson, R.T., Crowley, D., Tuveson, D.A., and Jacks, T. (2001). Somatic activation of the K-ras oncogene causes early onset lung cancer in mice. Nature 410, 1111\u20131116.","journal-title":"Nature"},{"key":"2088_CR18","doi-asserted-by":"publisher","first-page":"1086","DOI":"10.1096\/fj.11-185041","volume":"26","author":"N Klymiuk","year":"2012","unstructured":"Klymiuk, N., B\u00f6cker, W., Sch\u00f6nitzer, V., B\u00e4hr, A., Radic, T., Fr\u00f6hlich, T., W\u00fcnsch, A., Ke\u00dfler, B., Kurome, M., Schilling, E., et al. (2012). First inducible transgene expression in porcine large animal models. FASEB J 26, 1086\u20131099.","journal-title":"FASEB J"},{"key":"2088_CR19","doi-asserted-by":"publisher","first-page":"591","DOI":"10.1016\/j.immuni.2019.09.010","volume":"51","author":"M Kurachi","year":"2019","unstructured":"Kurachi, M., Ngiow, S.F., Kurachi, J., Chen, Z., and Wherry, E.J. (2019). Hidden caveat of inducible Cre recombinase. Immunity 51, 591\u2013592.","journal-title":"Immunity"},{"key":"2088_CR20","doi-asserted-by":"publisher","first-page":"1089","DOI":"10.1126\/science.1068228","volume":"295","author":"L Lai","year":"2002","unstructured":"Lai, L., Kolber-Simonds, D., Park, K.W., Cheong, H.T., Greenstein, J.L., Im, G.S., Samuel, M., Bonk, A., Rieke, A., Day, B.N., et al. (2002). Production of \u03b1-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 295, 1089\u20131092.","journal-title":"Science"},{"key":"2088_CR21","doi-asserted-by":"publisher","first-page":"743","DOI":"10.1038\/35093537","volume":"2","author":"M Lewandoski","year":"2001","unstructured":"Lewandoski, M. (2001). Conditional control of gene expression in the mouse. Nat Rev Genet 2, 743\u2013755.","journal-title":"Nat Rev Genet"},{"key":"2088_CR22","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1038\/cr.2014.15","volume":"24","author":"X Li","year":"2014","unstructured":"Li, X., Yang, Y., Bu, L., Guo, X., Tang, C., Song, J., Fan, N., Zhao, B., Ouyang, Z., Liu, Z., et al. (2014). Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing. Cell Res 24, 501\u2013504.","journal-title":"Cell Res"},{"key":"2088_CR23","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/B978-0-12-374814-0.00011-2","volume-title":"Methods in Cell Biology. Zebrafish: Genetics, Genomics and Informatics","author":"JA Lister","year":"2011","unstructured":"Lister, J.A. (2011). Use of phage PhiC31 integrase as a tool for zebrafish genome manipulation. In: Detrich, H.W., Westerfield, M., and Zon, L.I., eds. Methods in Cell Biology. Zebrafish: Genetics, Genomics and Informatics, 3rd Ed. New York: Academic Press. 195\u2013208.","edition":"3rd Ed"},{"key":"2088_CR24","doi-asserted-by":"publisher","first-page":"eabd5758","DOI":"10.1126\/scitranslmed.abd5758","volume":"13","author":"JK Lunney","year":"2021","unstructured":"Lunney, J.K., Van Goor, A., Walker, K.E., Hailstock, T., Franklin, J., and Dai, C. (2021). Importance of the pig as a human biomedical model. Sci Transl Med 13, eabd5758.","journal-title":"Sci Transl Med"},{"key":"2088_CR25","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.tim.2011.11.002","volume":"20","author":"F Meurens","year":"2012","unstructured":"Meurens, F., Summerfield, A., Nauwynck, H., Saif, L., and Gerdts, V. (2012). The pig: a model for human infectious diseases. Trends Microbiol 20, 50\u201357.","journal-title":"Trends Microbiol"},{"key":"2088_CR26","doi-asserted-by":"publisher","first-page":"447","DOI":"10.1097\/00000372-200412000-00001","volume":"26","author":"SJ Plumb","year":"2004","unstructured":"Plumb, S.J., Argenyi, Z.B., Stone, M.S., and De Young, B.R. (2004). Cytokeratin 5\/6 immunostaining in cutaneous adnexal neoplasms and metastatic adenocarcinoma. Am J Dermatopathol 26, 447\u2013451.","journal-title":"Am J Dermatopathol"},{"key":"2088_CR27","doi-asserted-by":"publisher","first-page":"14253","DOI":"10.1038\/srep14253","volume":"5","author":"J Ruan","year":"2015","unstructured":"Ruan, J., Li, H., Xu, K., Wu, T., Wei, J., Zhou, R., Liu, Z., Mu, Y., Yang, S., Ouyang, H., et al. (2015). Highly efficient CRISPR\/Cas9-mediated transgene knockin at the H11 locus in pigs. Sci Rep 5, 14253.","journal-title":"Sci Rep"},{"key":"2088_CR28","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1038\/ni0707-665","volume":"8","author":"M Schmidt-Supprian","year":"2007","unstructured":"Schmidt-Supprian, M., and Rajewsky, K. (2007). Vagaries of conditional gene targeting. Nat Immunol 8, 665\u2013668.","journal-title":"Nat Immunol"},{"key":"2088_CR29","doi-asserted-by":"publisher","first-page":"861","DOI":"10.1016\/j.cell.2007.11.019","volume":"131","author":"K Takahashi","year":"2007","unstructured":"Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. (2007). Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861\u2013872.","journal-title":"Cell"},{"key":"2088_CR30","doi-asserted-by":"publisher","first-page":"7902","DOI":"10.1073\/pnas.1019507108","volume":"108","author":"B Tasic","year":"2011","unstructured":"Tasic, B., Hippenmeyer, S., Wang, C., Gamboa, M., Zong, H., Chen-Tsai, Y., and Luo, L. (2011). Site-specific integrase-mediated transgenesis in mice via pronuclear injection. Proc Natl Acad Sci USA 108, 7902\u20137907.","journal-title":"Proc Natl Acad Sci USA"},{"key":"2088_CR31","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.gene.2012.11.016","volume":"515","author":"S Turan","year":"2013","unstructured":"Turan, S., Zehe, C., Kuehle, J., Qiao, J., and Bode, J. (2013). Recombinase-mediated cassette exchange (RMCE)\u2014a rapidly-expanding toolbox for targeted genomic modifications. Gene 515, 1\u201327.","journal-title":"Gene"},{"key":"2088_CR32","doi-asserted-by":"publisher","first-page":"11730","DOI":"10.1038\/s41598-019-48029-z","volume":"9","author":"S Vatansever","year":"2019","unstructured":"Vatansever, S., Erman, B., and G\u00fcm\u00fc\u015f, Z.H. (2019). Oncogenic G12D mutation alters local conformations and dynamics of K-Ras. Sci Rep 9, 11730.","journal-title":"Sci Rep"},{"key":"2088_CR33","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1186\/s13024-015-0036-5","volume":"10","author":"G Wang","year":"2015","unstructured":"Wang, G., Yang, H., Yan, S., Wang, C.E., Liu, X., Zhao, B., Ouyang, Z., Yin, P., Liu, Z., Zhao, Y., et al. (2015a). Cytoplasmic mislocalization of RNA splicing factors and aberrant neuronal gene splicing in TDP-43 transgenic pig brain. Mol Neurodegener 10, 42.","journal-title":"Mol Neurodegener"},{"key":"2088_CR34","doi-asserted-by":"publisher","first-page":"2061","DOI":"10.1101\/gr.222521.117","volume":"27","author":"K Wang","year":"2017","unstructured":"Wang, K., Jin, Q., Ruan, D., Yang, Y., Liu, Q., Wu, H., Zhou, Z., Ouyang, Z., Liu, Z., Zhao, Y., et al. (2017). Cre-dependent Cas9-expressing pigs enable efficient in vivo genome editing. Genome Res 27, 2061\u20132071.","journal-title":"Genome Res"},{"key":"2088_CR35","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1007\/s11248-014-9833-9","volume":"24","author":"Y Wang","year":"2015","unstructured":"Wang, Y., Yang, H.Q., Jiang, W., Fan, N.N., Zhao, B.T., Ou-Yang, Z., Liu, Z.M., Zhao, Y., Yang, D.S., Zhou, X.Y., et al. (2015b). Transgenic expression of human cytoxic T-lymphocyte associated antigen4-Immunoglobulin (hCTLA4Ig) by porcine skin for xenogeneic skin grafting. Transgenic Res 24, 199\u2013211.","journal-title":"Transgenic Res"},{"key":"2088_CR36","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1007\/s11248-018-0064-3","volume":"27","author":"KM Whitworth","year":"2018","unstructured":"Whitworth, K.M., Cecil, R., Benne, J.A., Redel, B.K., Spate, L.D., Samuel, M.S., Prather, R.S., and Wells, K.D. (2018). Zygote injection of RNA encoding Cre recombinase results in efficient removal of LoxP flanked neomycin cassettes in pigs. Transgenic Res 27, 167\u2013178.","journal-title":"Transgenic Res"},{"key":"2088_CR37","doi-asserted-by":"publisher","first-page":"3593","DOI":"10.1007\/s00018-018-2810-3","volume":"75","author":"H Wu","year":"2018","unstructured":"Wu, H., Liu, Q., Shi, H., Xie, J., Zhang, Q., Ouyang, Z., Li, N., Yang, Y., Liu, Z., Zhao, Y., et al. (2018). Engineering CRISPR\/Cpf1 with tRNA promotes genome editing capability in mammalian systems. Cell Mol Life Sci 75, 3593\u20133607.","journal-title":"Cell Mol Life Sci"},{"key":"2088_CR38","doi-asserted-by":"publisher","first-page":"2852","DOI":"10.1038\/s41467-019-10421-8","volume":"10","author":"J Xie","year":"2019","unstructured":"Xie, J., Ge, W., Li, N., Liu, Q., Chen, F., Yang, X., Huang, X., Ouyang, Z., Zhang, Q., Zhao, Y., et al. (2019). Efficient base editing for multiple genes and loci in pigs using base editors. Nat Commun 10, 2852.","journal-title":"Nat Commun"},{"key":"2088_CR39","doi-asserted-by":"publisher","first-page":"989","DOI":"10.1016\/j.cell.2018.03.005","volume":"173","author":"S Yan","year":"2018","unstructured":"Yan, S., Tu, Z., Liu, Z., Fan, N., Yang, H., Yang, S., Yang, W., Zhao, Y., Ouyang, Z., Lai, C., et al. (2018). A Huntingtin knockin pig model recapitulates features of selective neurodegeneration in Huntington\u2019s disease. Cell 173, 989\u20131002.e13.","journal-title":"Cell"},{"key":"2088_CR40","doi-asserted-by":"publisher","first-page":"3983","DOI":"10.1093\/hmg\/ddq313","volume":"19","author":"D Yang","year":"2010","unstructured":"Yang, D., Wang, C.E., Zhao, B., Li, W., Ouyang, Z., Liu, Z., Yang, H., Fan, P., O\u2019Neill, A., Gu, W., et al. (2010). Expression of Huntington\u2019s disease protein results in apoptotic neurons in the brains of cloned transgenic pigs. Hum Mol Genet 19, 3983\u20133994.","journal-title":"Hum Mol Genet"},{"key":"2088_CR41","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1038\/cr.2014.25","volume":"24","author":"H Yang","year":"2014","unstructured":"Yang, H., Wang, G., Sun, H., Shu, R., Liu, T., Wang, C.E., Liu, Z., Zhao, Y., Zhao, B., Ouyang, Z., et al. (2014). Species-dependent neuropathology in transgenic SOD1 pigs. Cell Res 24, 464\u2013481.","journal-title":"Cell Res"},{"key":"2088_CR42","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1093\/nsr\/nwz013","volume":"6","author":"J Zhao","year":"2019","unstructured":"Zhao, J., Lai, L., Ji, W., and Zhou, Q. (2019). Genome editing in large animals: current status and future prospects. Natl Sci Rev 6, 402\u2013420.","journal-title":"Natl Sci Rev"},{"key":"2088_CR43","doi-asserted-by":"publisher","first-page":"E9474","DOI":"10.1073\/pnas.1707853114","volume":"114","author":"Q Zheng","year":"2017","unstructured":"Zheng, Q., Lin, J., Huang, J., Zhang, H., Zhang, R., Zhang, X., Cao, C., Hambly, C., Qin, G., Yao, J., et al. (2017). Reconstitution of UCP1 using CRISPR\/Cas9 in the white adipose tissue of pigs decreases fat deposition and improves thermogenic capacity. Proc Natl Acad Sci USA 114, E9474\u2013E9482.","journal-title":"Proc Natl Acad Sci USA"},{"key":"2088_CR44","first-page":"927","volume":"17","author":"X Zhou","year":"2019","unstructured":"Zhou, X., Li, G., Wang, D., Sun, X., and Li, X. (2019). Cytokeratin expression in epidermal stem cells in skin adnexal tumors. Oncol Lett 17, 927\u2013932.","journal-title":"Oncol Lett"}],"container-title":["Science China Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11427-021-2088-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11427-021-2088-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11427-021-2088-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,11,21]],"date-time":"2022-11-21T08:23:59Z","timestamp":1669019039000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11427-021-2088-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,18]]},"references-count":44,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["2088"],"URL":"https:\/\/doi.org\/10.1007\/s11427-021-2088-1","relation":{},"ISSN":["1674-7305","1869-1889"],"issn-type":[{"value":"1674-7305","type":"print"},{"value":"1869-1889","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,18]]},"assertion":[{"value":"5 November 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 January 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"18 May 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"<b>Compliance and ethics<\/b> The author(s) declare that they have no conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics"}}]}}