{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T15:54:50Z","timestamp":1780761290073,"version":"3.54.1"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T00:00:00Z","timestamp":1654732800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T00:00:00Z","timestamp":1654732800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci. China Life Sci."],"published-print":{"date-parts":[[2022,12]]},"DOI":"10.1007\/s11427-021-2095-0","type":"journal-article","created":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T08:03:07Z","timestamp":1655107387000},"page":"2517-2526","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Structural and functional analysis of a potent human neutralizing antibody against enterovirus A71"],"prefix":"10.1007","volume":"65","author":[{"given":"Zhe","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linlin","family":"Bao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangli","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianjiao","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xurong","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fengdi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feifei","family":"Qi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenbo","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongrong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,9]]},"reference":[{"key":"2095_CR1","doi-asserted-by":"publisher","first-page":"7978","DOI":"10.1073\/pnas.88.18.7978","volume":"88","author":"CF Barbas 3rd","year":"1991","unstructured":"Barbas, C.F. 3rd, Kang, A.S., Lerner, R.A., and Benkovic, S.J. (1991). Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc Natl Acad Sci USA 88, 7978\u20137982.","journal-title":"Proc Natl Acad Sci USA"},{"key":"2095_CR2","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1002\/rmv.538","volume":"17","author":"JM Bible","year":"2007","unstructured":"Bible, J.M., Pantelidis, P., Chan, P.K.S., and Tong, C.Y.W. (2007). Genetic evolution of enterovirus 71: epidemiological and pathological implications. Rev Med Virol 17, 371\u2013379.","journal-title":"Rev Med Virol"},{"key":"2095_CR3","doi-asserted-by":"publisher","first-page":"332","DOI":"10.1016\/j.jsb.2005.03.009","volume":"150","author":"JAG Briggs","year":"2005","unstructured":"Briggs, J.A.G., Huiskonen, J.T., Fernando, K.V., Gilbert, R.J.C., Scotti, P., Butcher, S.J., and Fuller, S.D. (2005). Classification and three-dimensional reconstruction of unevenly distributed or symmetry mismatched features of icosahedral particles. J Struct Biol 150, 332\u2013339.","journal-title":"J Struct Biol"},{"key":"2095_CR4","doi-asserted-by":"publisher","first-page":"374","DOI":"10.1007\/s13238-020-00715-9","volume":"11","author":"W Chen","year":"2020","unstructured":"Chen, W., Xiao, H., Wang, X., Song, S., Han, Z., Li, X., Yang, F., Wang, L., Song, J., Liu, H., et al. (2020). Structural changes of a bacteriophage upon DNA packaging and maturation. Protein Cell 11, 374\u2013379.","journal-title":"Protein Cell"},{"key":"2095_CR5","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1107\/S0907444910007493","volume":"66","author":"P Emsley","year":"2010","unstructured":"Emsley, P., Lohkamp, B., Scott, W.G., and Cowtan, K. (2010). Features and development of Coot. Acta Crystlogr D Biol Crystlogr 66, 486\u2013501.","journal-title":"Acta Crystlogr D Biol Crystlogr"},{"key":"2095_CR6","doi-asserted-by":"publisher","first-page":"5253","DOI":"10.1038\/s41467-020-19013-3","volume":"11","author":"KYA Huang","year":"2020","unstructured":"Huang, K.Y.A., Zhou, D., Fry, E.E., Kotecha, A., Huang, P.N., Yang, S.L., Tsao, K.C., Huang, Y.C., Lin, T.Y., Ren, J., et al. (2020). Structural and functional analysis of protective antibodies targeting the threefold plateau of enterovirus 71. Nat Commun 11, 5253.","journal-title":"Nat Commun"},{"key":"2095_CR7","doi-asserted-by":"publisher","first-page":"8843","DOI":"10.1038\/ncomms9843","volume":"6","author":"SL Ilca","year":"2015","unstructured":"Ilca, S.L., Kotecha, A., Sun, X., Poranen, M.M., Stuart, D.I., and Huiskonen, J.T. (2015). Localized reconstruction of subunits from electron cryomicroscopy images of macromolecular complexes. Nat Commun 6, 8843.","journal-title":"Nat Commun"},{"key":"2095_CR8","doi-asserted-by":"publisher","first-page":"46402","DOI":"10.1038\/srep46402","volume":"7","author":"Q Jia","year":"2017","unstructured":"Jia, Q., Ng, Q., Chin, W., Meng, T., Chow, V.T.K., Wang, C.I., Kwang, J., and He, F. (2017). Effective in vivo therapeutic IgG antibody against VP3 of enterovirus 71 with receptor-competing activity. Sci Rep 7, 46402.","journal-title":"Sci Rep"},{"key":"2095_CR9","doi-asserted-by":"publisher","first-page":"e2895","DOI":"10.1371\/journal.pntd.0002895","volume":"8","author":"TK Kiener","year":"2014","unstructured":"Kiener, T.K., Jia, Q., Meng, T., Chow, V.T.K., and Kwang, J. (2014). A novel universal neutralizing monoclonal antibody against enterovirus 71 that targets the highly conserved \u201cKnob\u201d region of VP3 protein. PLoS Negl Trop Dis 8, e2895.","journal-title":"PLoS Negl Trop Dis"},{"key":"2095_CR10","doi-asserted-by":"publisher","first-page":"12084","DOI":"10.1128\/JVI.02189-15","volume":"89","author":"Z Ku","year":"2015","unstructured":"Ku, Z., Ye, X., Shi, J., Wang, X., Liu, Q., and Huang, Z. (2015). Single neutralizing monoclonal antibodies targeting the VP1 GH loop of enterovirus 71 inhibit both virus attachment and internalization during viral entry. J Virol 89, 12084\u201312095.","journal-title":"J Virol"},{"key":"2095_CR11","doi-asserted-by":"publisher","first-page":"11363","DOI":"10.1128\/JVI.01926-13","volume":"87","author":"H Lee","year":"2013","unstructured":"Lee, H., Cifuente, J.O., Ashley, R.E., Conway, J.F., Makhov, A.M., Tano, Y., Shimizu, H., Nishimura, Y., and Hafenstein, S. (2013). A strain-specific epitope of enterovirus 71 identified by cryo-electron microscopy of the complex with Fab from neutralizing antibody. J Virol 87, 11363\u201311370.","journal-title":"J Virol"},{"key":"2095_CR12","doi-asserted-by":"publisher","first-page":"584","DOI":"10.1038\/nmeth.2472","volume":"10","author":"X Li","year":"2013","unstructured":"Li, X., Mooney, P., Zheng, S., Booth, C.R., Braunfeld, M.B., Gubbens, S., Agard, D.A., and Cheng, Y. (2013). Electron counting and beam-induced motion correction enable near-atomic-resolution single-particle cryo-EM. Nat Methods 10, 584\u2013590.","journal-title":"Nat Methods"},{"key":"2095_CR13","doi-asserted-by":"publisher","first-page":"1800590","DOI":"10.2807\/1560-7917.ES.2018.23.46.1800590","volume":"23","author":"LNT Nhan","year":"2018","unstructured":"Nhan, L.N.T., Hong, N.T.T., Nhu, L.N.T., Nguyet, L.A., Ny, N.T.H., Thanh, T.T., Han, D.D.K., Van, H.M.T., Thwaites, C.L., Hien, T.T., et al. (2018). Severe enterovirus A71 associated hand, foot and mouth disease, Vietnam, 2018: preliminary report of an impending outbreak. Eurosurveillance 23, 1800590.","journal-title":"Eurosurveillance"},{"key":"2095_CR14","doi-asserted-by":"publisher","first-page":"e1001174","DOI":"10.1371\/journal.ppat.1001174","volume":"6","author":"Y Nishimura","year":"2010","unstructured":"Nishimura, Y., Wakita, T., and Shimizu, H. (2010). Tyrosine sulfation of the amino terminus of PSGL-1 is critical for enterovirus 71 infection. PLoS Pathog 6, e1001174.","journal-title":"PLoS Pathog"},{"key":"2095_CR15","doi-asserted-by":"publisher","first-page":"e1003511","DOI":"10.1371\/journal.ppat.1003511","volume":"9","author":"Y Nishimura","year":"2013","unstructured":"Nishimura, Y., Lee, H., Hafenstein, S., Kataoka, C., Wakita, T., Bergelson, J.M., and Shimizu, H. (2013). Enterovirus 71 binding to PSGL-1 on leukocytes: VP1\u2013145 acts as a molecular switch to control receptor interaction. PLoS Pathog 9, e1003511.","journal-title":"PLoS Pathog"},{"key":"2095_CR16","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1038\/nm.1961","volume":"71","author":"Y Nishimura","year":"2009","unstructured":"Nishimura, Y., Shimojima, M., Tano, Y., Miyamura, T., Wakita, T., and Shimizu, H. (2009). Human P-selectin glycoprotein ligand-1 is a functional receptor for enterovirus 71. Nat Med 15, 794\u2013797.","journal-title":"Nat Med 15"},{"key":"2095_CR17","doi-asserted-by":"publisher","first-page":"1941","DOI":"10.1128\/JVI.73.3.1941-1948.1999","volume":"73","author":"MS Oberste","year":"1999","unstructured":"Oberste, M.S., Maher, K., Kilpatrick, D.R., and Pallansch, M.A. (1999). Molecular evolution of the human enteroviruses: correlation of serotype with VP1 sequence and application to picornavirus classification. J Virol 73, 1941\u20131948.","journal-title":"J Virol"},{"key":"2095_CR18","doi-asserted-by":"publisher","first-page":"1097","DOI":"10.1016\/S1474-4422(10)70209-X","volume":"9","author":"MH Ooi","year":"2010","unstructured":"Ooi, M.H., Wong, S.C., Lewthwaite, P., Cardosa, M.J., and Solomon, T. (2010). Clinical features, diagnosis, and management of enterovirus 71. Lancet Neurol 9, 1097\u20131105.","journal-title":"Lancet Neurol"},{"key":"2095_CR19","doi-asserted-by":"publisher","first-page":"1274","DOI":"10.1126\/science.1218713","volume":"336","author":"P Plevka","year":"2012","unstructured":"Plevka, P., Perera, R., Cardosa, J., Kuhn, R.J., and Rossmann, M.G. (2012). Crystal structure of human enterovirus 71. Science 336, 1274.","journal-title":"Science"},{"key":"2095_CR20","doi-asserted-by":"publisher","first-page":"2000","DOI":"10.1096\/fj.02-0281fje","volume":"16","author":"C Rader","year":"2002","unstructured":"Rader, C., Popkov, M., Neves, J.A., and Barbas, C.F. 3rd (2002). Integrin av\u03b23-targeted therapy for Kaposi\u2019s sarcoma with an in vitro-evolved antibody. FASEB J 16, 2000\u20132002.","journal-title":"FASEB J"},{"key":"2095_CR21","first-page":"43","volume":"1","author":"S Ranganathan","year":"2002","unstructured":"Ranganathan, S., Singh, S., Poh, C.L., and Chow, V.T. (2002). The hand, foot and mouth disease virus capsid: sequence analysis and prediction of antigenic sites from homology modelling. Appl Bioinformatics 1, 43\u201352.","journal-title":"Appl Bioinformatics"},{"key":"2095_CR22","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1016\/j.cell.2017.04.024","volume":"169","author":"DF Robbiani","year":"2017","unstructured":"Robbiani, D.F., Bozzacco, L., Keeffe, J.R., Khouri, R., Olsen, P.C., Gazumyan, A., Schaefer-Babajew, D., Avila-Rios, S., Nogueira, L., Patel, R., et al. (2017). Recurrent potent human neutralizing antibodies to Zika virus in Brazil and Mexico. Cell 169, 597\u2013609.e11.","journal-title":"Cell"},{"key":"2095_CR23","doi-asserted-by":"publisher","first-page":"216","DOI":"10.1016\/j.jsb.2015.08.008","volume":"192","author":"A Rohou","year":"2015","unstructured":"Rohou, A., and Grigorieff, N. (2015). CTFFIND4: fast and accurate defocus estimation from electron micrographs. J Struct Biol 192, 216\u2013221.","journal-title":"J Struct Biol"},{"key":"2095_CR24","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1016\/j.jsb.2012.09.006","volume":"180","author":"SHW Scheres","year":"2012","unstructured":"Scheres, S.H.W. (2012). RELION: implementation of a Bayesian approach to cryo-EM structure determination. J Struct Biol 180, 519\u2013530.","journal-title":"J Struct Biol"},{"key":"2095_CR25","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1093\/infdis\/129.3.304","volume":"129","author":"NJ Schmidt","year":"1974","unstructured":"Schmidt, N.J., Lennette, E.H., and Ho, H.H. (1974). An apparently new enterovirus isolated from patients with disease of the central nervous system. J Infect Dis 129, 304\u2013309.","journal-title":"J Infect Dis"},{"key":"2095_CR26","doi-asserted-by":"publisher","first-page":"1900","DOI":"10.1128\/JVI.03098-14","volume":"89","author":"KL Shingler","year":"2015","unstructured":"Shingler, K.L., Cifuente, J.O., Ashley, R.E., Makhov, A.M., Conway, J.F., and Hafenstein, S. (2015). The enterovirus 71 procapsid binds neutralizing antibodies and rescues virus infection in vitro. J Virol 89, 1900\u20131908.","journal-title":"J Virol"},{"key":"2095_CR27","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1128\/JVI.02226-12","volume":"87","author":"CW Tan","year":"2012","unstructured":"Tan, C.W., Poh, C.L., Sam, I.C., and Chan, Y.F. (2012). Enterovirus 71 uses cell surface heparan sulfate glycosaminoglycan as an attachment receptor. J Virol 87, 611\u2013620.","journal-title":"J Virol"},{"key":"2095_CR28","doi-asserted-by":"publisher","first-page":"38","DOI":"10.1016\/j.jsb.2006.05.009","volume":"157","author":"G Tang","year":"2007","unstructured":"Tang, G., Peng, L., Baldwin, P.R., Mann, D.S., Jiang, W., Rees, I., and Ludtke, S.J. (2007). EMAN2: an extensible image processing suite for electron microscopy. J Struct Biol 157, 38\u201346.","journal-title":"J Struct Biol"},{"key":"2095_CR29","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.chom.2018.12.004","volume":"25","author":"AZ Wec","year":"2019","unstructured":"Wec, A.Z., Bornholdt, Z.A., He, S., Herbert, A.S., Goodwin, E., Wirchnianski, A.S., Gunn, B.M., Zhang, Z., Zhu, W., Liu, G., et al. (2019). Development of a human antibody cocktail that deploys multiple functions to confer pan-ebolavirus protection. Cell Host Microbe 25, 39\u201348.e5.","journal-title":"Cell Host Microbe"},{"key":"2095_CR30","doi-asserted-by":"publisher","first-page":"798","DOI":"10.1038\/nm.1992","volume":"15","author":"S Yamayoshi","year":"2009","unstructured":"Yamayoshi, S., Yamashita, Y., Li, J., Hanagata, N., Minowa, T., Takemura, T., and Koike, S. (2009). Scavenger receptor B2 is a cellular receptor for enterovirus 71. Nat Med 15, 798\u2013801.","journal-title":"Nat Med"},{"key":"2095_CR31","doi-asserted-by":"publisher","first-page":"e1005454","DOI":"10.1371\/journal.ppat.1005454","volume":"12","author":"X Ye","year":"2016","unstructured":"Ye, X., Fan, C., Ku, Z., Zuo, T., Kong, L., Zhang, C., Shi, J., Liu, Q., Chen, T., Zhang, Y., et al. (2016). Structural basis for recognition of human enterovirus 71 by a bivalent broadly neutralizing monoclonal antibody. PLoS Pathog 12, e1005454.","journal-title":"PLoS Pathog"},{"key":"2095_CR32","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1038\/s41586-020-2548-6","volume":"584","author":"SJ Zost","year":"2020","unstructured":"Zost, S.J., Gilchuk, P., Case, J.B., Binshtein, E., Chen, R.E., Nkolola, J.P., Sch\u00e4fer, A., Reidy, J.X., Trivette, A., Nargi, R.S., et al. (2020). Potently neutralizing and protective human antibodies against SARS-CoV-2. Nature 584, 443\u2013449.","journal-title":"Nature"}],"container-title":["Science China Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11427-021-2095-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11427-021-2095-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11427-021-2095-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T13:03:27Z","timestamp":1744203807000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11427-021-2095-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,9]]},"references-count":32,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["2095"],"URL":"https:\/\/doi.org\/10.1007\/s11427-021-2095-0","relation":{},"ISSN":["1674-7305","1869-1889"],"issn-type":[{"value":"1674-7305","type":"print"},{"value":"1869-1889","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,6,9]]},"assertion":[{"value":"21 November 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 March 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 June 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}