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These genes predominantly supported\n                    <jats:italic>de novo<\/jats:italic>\n                    fatty acid synthesis, such as\n                    <jats:italic>FASN<\/jats:italic>\n                    and\n                    <jats:italic>ACSL3<\/jats:italic>\n                    . Furthermore, we showed that an isoform switch to the proximal poly(A) site of these genes depended on the androgen receptor, and the expression of cancer-associated genes was upregulated by the escape of miRNA-regulated repression machinery. To address the role of key gene shortening in PCa, we prepared 22RV1-FS cells missing the distal poly(A) signal of\n                    <jats:italic>FASN<\/jats:italic>\n                    in the AR\n                    <jats:sup>+<\/jats:sup>\n                    PCa cell line 22RV1 using CRISPR\/Cas9 technology. As expected, the edited 22RV1-FS cells overexpressed\n                    <jats:italic>FASN<\/jats:italic>\n                    mRNA and protein and were inclined to cell proliferation\n                    <jats:italic>in vitro<\/jats:italic>\n                    and tumorigenesis\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Interestingly, we found that FASN transcripts with a shortened 3\u2032UTR were significantly increased in advanced PCa and castration-resistant prostate cancer compared with benign prostate hyperplasia, suggesting a possible association between usage of the proximal poly(A) site and disease progression. Therefore, our study highlights the importance of the APA mechanism in response to DHT stimulation in PCa cells and provides a novel regulatory mechanism through which DHT-induced APA causes altered expression of\n                    <jats:italic>de novo<\/jats:italic>\n                    lipogenesis genes, with a possible association with the progression of PCa.\n                  <\/jats:p>","DOI":"10.1007\/s11427-024-2740-7","type":"journal-article","created":{"date-parts":[[2025,7,11]],"date-time":"2025-07-11T04:39:53Z","timestamp":1752208793000},"page":"3217-3230","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Androgen induces 3\u2032UTR shortening of de novo lipogenesis genes by alternative polyadenylation in prostate cancer cells"],"prefix":"10.1007","volume":"68","author":[{"given":"Wei-Jie","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Mei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong-Yin","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"An-Long","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Gui","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Ouyang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Newton O.","family":"Otecko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben-Xia","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teng-Fei","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong-Dong","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Ping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,8]]},"reference":[{"key":"2740_CR1","doi-asserted-by":"publisher","first-page":"11706","DOI":"10.1038\/ncomms11706","volume":"7","author":"SE Abdel-Ghany","year":"2016","unstructured":"Abdel-Ghany, S.E., Hamilton, M., Jacobi, J.L., Ngam, P., Devitt, N., Schilkey, F., Ben-Hur, A., and Reddy, A.S.N. 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