{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:03:38Z","timestamp":1789585418906,"version":"build-2803163510"},"reference-count":58,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T00:00:00Z","timestamp":1751414400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci. China Life Sci."],"published-print":{"date-parts":[[2025,10]]},"DOI":"10.1007\/s11427-024-2907-7","type":"journal-article","created":{"date-parts":[[2025,7,3]],"date-time":"2025-07-03T23:01:35Z","timestamp":1751583695000},"page":"2965-2981","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["(Z)-Ligustilide alleviates intervertebral disc degeneration by suppressing nucleus pulposus cell pyroptosis via Atg5\/NLRP3 axis"],"prefix":"10.1007","volume":"68","author":[{"given":"Jiale","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyang","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Hua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongwei","family":"Ji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongkang","family":"Deng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dechun","family":"Geng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiexing","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiqing","family":"Mao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,2]]},"reference":[{"key":"2907_CR1","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.jare.2020.08.004","volume":"28","author":"A Al Mamun","year":"2021","unstructured":"Al Mamun, A., Wu, Y., Monalisa, I., Jia, C., Zhou, K., Munir, F., and Xiao, J. (2021). Role of pyroptosis in spinal cord injury and its therapeutic implications. J Adv Res 28, 97\u2013109.","journal-title":"J Adv Res"},{"key":"2907_CR2","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1038\/s41577-019-0228-2","volume":"20","author":"P Broz","year":"2020","unstructured":"Broz, P., Pelegrm, P., and Shao, F. (2020). The gasdermins, a protein family executing cell death and inflammation. Nat Rev Immunol 20, 143\u2013157.","journal-title":"Nat Rev Immunol"},{"key":"2907_CR3","doi-asserted-by":"publisher","first-page":"2768","DOI":"10.1016\/j.apsb.2021.02.006","volume":"11","author":"BE Burdette","year":"2021","unstructured":"Burdette, B.E., Esparza, A.N., Zhu, H., and Wang, S. (2021). Gasdermin D in pyroptosis. Acta Pharm Sin B 11, 2768\u20132782.","journal-title":"Acta Pharm Sin B"},{"key":"2907_CR4","doi-asserted-by":"publisher","first-page":"105138","DOI":"10.1016\/j.chemphyslip.2021.105138","volume":"241","author":"Y Cao","year":"2021","unstructured":"Cao, Y., Dong, Z., Yang, D., Ma, X., and Wang, X. (2021). Alleviation of glucolipotoxicity-incurred cardiomyocyte dysfunction by Z-ligustilide involves in the suppression of oxidative insult, inflammation and fibrosis. Chem Phys Lipids 241, 105138.","journal-title":"Chem Phys Lipids"},{"key":"2907_CR5","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1038\/s41413-020-0087-2","volume":"8","author":"F Chen","year":"2020","unstructured":"Chen, F., Jiang, G., Liu, H., Li, Z., Pei, Y., Wang, H., Pan, H., Cui, H., Long, J., Wang, J., et al. (2020). Melatonin alleviates intervertebral disc degeneration by disrupting the IL-1\u03b2\/NF-\u03baB-NLRP3 inflammasome positive feedback loop. Bone Res 8, 10.","journal-title":"Bone Res"},{"key":"2907_CR6","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1016\/j.biopha.2017.04.093","volume":"91","author":"J Chen","year":"2017","unstructured":"Chen, J., Xuan, J., Gu, Y.T., Shi, K.S., Xie, J.J., Chen, J.X., Zheng, Z.M., Chen, Y., Chen, X.B., Wu, Y.S., et al. (2017). Celastrol reduces IL-1\u03b2 induced matrix catabolism, oxidative stress and inflammation in human nucleus pulposus cells and attenuates rat intervertebral disc degeneration in vivo. Biomed Pharmacother 91, 208\u2013219.","journal-title":"Biomed Pharmacother"},{"key":"2907_CR7","doi-asserted-by":"publisher","first-page":"102323","DOI":"10.1016\/j.arr.2024.102323","volume":"98","author":"X Chen","year":"2024","unstructured":"Chen, X., Zhang, A., Zhao, K., Gao, H., Shi, P., Chen, Y., Cheng, Z., Zhou, W., and Zhang, Y. (2024). The role of oxidative stress in intervertebral disc degeneration: mechanisms and therapeutic implications. Ageing Res Rev 98, 102323.","journal-title":"Ageing Res Rev"},{"key":"2907_CR8","doi-asserted-by":"publisher","first-page":"1206513","DOI":"10.3389\/fimmu.2023.1206513","volume":"14","author":"Y Chen","year":"2023","unstructured":"Chen, Y., Peng, F., Yang, C., Hou, H., Xing, Z., Chen, J., Liu, L., Peng, C., and Li, D. (2023). SIRT1 activation by 2,3,5,6-tetramethylpyrazine alleviates neuroinflammation via inhibiting M1 microglia polarization. Front Immunol 14, 1206513.","journal-title":"Front Immunol"},{"key":"2907_CR9","doi-asserted-by":"publisher","first-page":"2006","DOI":"10.1016\/S0140-6736(20)32340-0","volume":"396","author":"A Cieza","year":"2021","unstructured":"Cieza, A., Causey, K., Kamenov, K., Hanson, S.W., Chatterji, S., and Vos, T. (2021). Global estimates of the need for rehabilitation based on the Global Burden of Disease study 2019: a systematic analysis for the Global Burden of Disease Study 2019. Lancet 396, 2006\u20132017.","journal-title":"Lancet"},{"key":"2907_CR10","doi-asserted-by":"publisher","first-page":"653","DOI":"10.1016\/j.tips.2022.04.003","volume":"43","author":"RC Coll","year":"2022","unstructured":"Coll, R.C., Schroder, K., and Pelegrin, P. (2022). NLRP3 and pyroptosis blockers for treating inflammatory diseases. Trends Pharmacol Sci 43, 653\u2013668.","journal-title":"Trends Pharmacol Sci"},{"key":"2907_CR11","doi-asserted-by":"publisher","first-page":"873","DOI":"10.1080\/15548627.2022.2107314","volume":"19","author":"Q Di","year":"2023","unstructured":"Di, Q., Zhao, X., Tang, H., Li, X., Xiao, Y., Wu, H., Wu, Z., Quan, J., and Chen, W. (2023). USP22 suppresses the NLRP3 inflammasome by degrading NLRP3 via ATG5-dependent autophagy. Autophagy 19, 873\u2013885.","journal-title":"Autophagy"},{"key":"2907_CR12","doi-asserted-by":"publisher","first-page":"2368","DOI":"10.1016\/S0140-6736(18)30489-6","volume":"391","author":"NE Foster","year":"2018","unstructured":"Foster, N.E., Anema, J.R., Cherkin, D., Chou, R., Cohen, S.P., Gross, D.P., Ferreira, P. H., Fritz, J.M., Koes, B.W., Peul, W., et al. (2018). Prevention and treatment of low back pain: evidence, challenges, and promising directions. Lancet 391, 2368\u20132383.","journal-title":"Lancet"},{"key":"2907_CR13","doi-asserted-by":"publisher","first-page":"588","DOI":"10.1007\/s11427-020-1939-1","volume":"65","author":"G Ge","year":"2022","unstructured":"Ge, G., Bai, J., Wang, Q., Liang, X., Tao, H., Chen, H., Wei, M., Niu, J., Yang, H., Xu, Y., et al. (2022). Punicalagin ameliorates collagen-induced arthritis by down-regulating M1 macrophage and pyroptosis via NF-\u03baB signaling pathway. Sci China Life Sci 65, 588\u2013603.","journal-title":"Sci China Life Sci"},{"key":"2907_CR14","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1016\/j.joca.2021.02.204","volume":"29","author":"CY Gong","year":"2021","unstructured":"Gong, C.Y., Cheng, P., and Zhang, H.L. (2021). Roles of NLRP3 inflammasome in intervertebral disc degeneration. Osteoarthritis Cartilage 29, 793\u2013801.","journal-title":"Osteoarthritis Cartilage"},{"key":"2907_CR15","doi-asserted-by":"publisher","first-page":"284","DOI":"10.1186\/s12974-023-02959-8","volume":"20","author":"X Huang","year":"2023","unstructured":"Huang, X., Ye, C., Zhao, X., Tong, Y., Lin, W., Huang, Q., Zheng, Y., Wang, J., Zhang, A., and Mo, Y. (2023). TRIM45 aggravates microglia pyroptosis via Atg5\/NLRP3 axis in septic encephalopathy. J Neuroinflamm 20, 284.","journal-title":"J Neuroinflamm"},{"key":"2907_CR16","doi-asserted-by":"publisher","first-page":"3965","DOI":"10.3390\/ijms22083965","volume":"22","author":"M Ito","year":"2021","unstructured":"Ito, M., Yurube, T., Kanda, Y., Kakiuchi, Y., Takeoka, Y., Takada, T., Kuroda, R., and Kakutani, K. (2021). Inhibition of autophagy at different stages by ATG5 knockdown and chloroquine supplementation enhances consistent human disc cellular apoptosis and senescence induction rather than extracellular matrix catabolism. Int J Mol Sci 22, 3965.","journal-title":"Int J Mol Sci"},{"key":"2907_CR17","doi-asserted-by":"publisher","first-page":"115336","DOI":"10.1016\/j.taap.2020.115336","volume":"410","author":"X Jiang","year":"2021","unstructured":"Jiang, X., Zhao, W., Zhu, F., Wu, H., Ding, X., Bai, J., Zhang, X., and Qian, M. (2021). Ligustilide inhibits the proliferation of non-small cell lung cancer via glycolytic metabolism. Toxicol Appl Pharmacol 410, 115336.","journal-title":"Toxicol Appl Pharmacol"},{"key":"2907_CR18","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1001\/jama.2017.7918","volume":"318","author":"JNS Juch","year":"2017","unstructured":"Juch, J.N.S., Maas, E.T., Ostelo, R.W.J.G., Groeneweg, J.G., Kallewaard, J.W., Koes, B. W., Verhagen, A.P., van Dongen, J.M., Huygen, F.J.P.M., and van Tulder, M.W. (2017). Effect of radiofrequency denervation on pain intensity among patients with chronic low back pain: the mint randomized clinical trials. JAMA 318, 68.","journal-title":"JAMA"},{"key":"2907_CR19","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1080\/15548627.2020.1797280","volume":"17","author":"DJ Klionsky","year":"2021","unstructured":"Klionsky, D.J., Abdel-Aziz, A.K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M.H., Abudu, Y.P., Acevedo-Arozena, A., et al. (2021). Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition). Autophagy 17, 1\u2013382.","journal-title":"Autophagy"},{"key":"2907_CR20","doi-asserted-by":"publisher","first-page":"110486","DOI":"10.1016\/j.intimp.2023.110486","volume":"121","author":"G Li","year":"2023","unstructured":"Li, G., Hu, C., Liu, Y., and Lin, H. (2023). Ligustilide, a novel SIRT1 agonist, alleviates lipopolysaccharide-induced acute lung injury through deacetylation of NICD. Int Immunopharmacol 121, 110486.","journal-title":"Int Immunopharmacol"},{"key":"2907_CR21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12974-020-02040-8","volume":"18","author":"Y Li","year":"2021","unstructured":"Li, Y., Song, W., Tong, Y., Zhang, X., Zhao, J., Gao, X., Yong, J., and Wang, H. (2021). Isoliquiritin ameliorates depression by suppressing NLRP3-mediated pyroptosis via miRNA-27a\/SYK\/NF-\u03baB axis. J Neuroinflamm 18, 1.","journal-title":"J Neuroinflamm"},{"key":"2907_CR22","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1007\/s11427-023-2587-2","volume":"68","author":"ZM Lin","year":"2025","unstructured":"Lin, Z.M., Xiang, M., Wei, W.H., Fan, S.H., Chen, L., Wang, J., Yang, X.Q., Yang, C.H., He, S.J., and Zuo, J.P. (2025). Identification of a dual functional betulinic acid analog for the treatment of osteoarthritis by phenotypic screening. Sci China Life Sci 68, 300\u2013302.","journal-title":"Sci China Life Sci"},{"key":"2907_CR23","doi-asserted-by":"publisher","first-page":"1804","DOI":"10.3390\/biom12121804","volume":"12","author":"J Luo","year":"2022","unstructured":"Luo, J., Yang, Y., Wang, X., Chang, X., and Fu, S. (2022). Role of pyroptosis in intervertebral disc degeneration and its therapeutic implications. Biomolecules 12, 1804.","journal-title":"Biomolecules"},{"key":"2907_CR24","doi-asserted-by":"publisher","first-page":"154720","DOI":"10.1016\/j.phymed.2023.154720","volume":"112","author":"Z Niu","year":"2023","unstructured":"Niu, Z., Tang, G., Wang, X., Yang, X., Zhao, Y., Wang, Y., Liu, Q., Zhang, F., Zhao, Y., Ding, X., et al. (2023). Trigonochinene E promotes lysosomal biogenesis and enhances autophagy via TFEB\/TFE3 in human degenerative NP cells against oxidative stress. Phytomedicine 112, 154720.","journal-title":"Phytomedicine"},{"key":"2907_CR25","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1016\/j.bbi.2024.02.001","volume":"117","author":"A Oladapo","year":"2024","unstructured":"Oladapo, A., Jackson, T., Menolascino, J., and Periyasamy, P. (2024). Role of pyroptosis in the pathogenesis of various neurological diseases. Brain Behav Immun 117, 428\u2013446.","journal-title":"Brain Behav Immun"},{"key":"2907_CR26","doi-asserted-by":"publisher","first-page":"1180","DOI":"10.3109\/13880209.2011.576346","volume":"49","author":"X Ran","year":"2011","unstructured":"Ran, X., Ma, L., Peng, C., Zhang, H., and Qin, L.P. (2011). Ligusticum chuanxiong Hort: a review of chemistry and pharmacology. Pharm Biol 49, 1180\u20131189.","journal-title":"Pharm Biol"},{"key":"2907_CR27","doi-asserted-by":"publisher","first-page":"803","DOI":"10.1038\/s41401-023-01212-5","volume":"45","author":"J Shao","year":"2024","unstructured":"Shao, J., Li, W., Sun, J., Zhuang, Z., Min, J., Long, X., Wu, G., Xu, H., and Liang, G. (2024). Britannin as a novel NLRP3 inhibitor, suppresses inflammasome activation in macrophages and alleviates NLRP3-related diseases in mice. Acta Pharmacol Sin 45, 803\u2013814.","journal-title":"Acta Pharmacol Sin"},{"key":"2907_CR28","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1016\/j.intimp.2014.02.007","volume":"19","author":"Y Shi","year":"2014","unstructured":"Shi, Y., Wang, D., Lu, L., Yin, Y., Wang, M., Li, C., Diao, J., Wang, Y., and Wei, L. (2014). Ligustilide prevents the apoptosis effects of tumour necrosis factor-alpha during C2C12 cell differentiation. Int Immunopharmacol 19, 358\u2013364.","journal-title":"Int Immunopharmacol"},{"key":"2907_CR29","doi-asserted-by":"publisher","first-page":"5481","DOI":"10.3390\/molecules27175481","volume":"27","author":"A Skrzypczak-Wiercioch","year":"2022","unstructured":"Skrzypczak-Wiercioch, A., and Salat, K. (2022). Lipopolysaccharide-induced model of neuroinflammation: mechanisms of action, research application and future directions for its use. Molecules 27, 5481.","journal-title":"Molecules"},{"key":"2907_CR30","doi-asserted-by":"publisher","first-page":"2313248","DOI":"10.1002\/adma.202313248","volume":"36","author":"K Sun","year":"2024","unstructured":"Sun, K., Yan, C., Dai, X., Shi, Y., Li, F., Chen, L., Sun, J., Chen, Y., and Shi, J. (2024a). Catalytic nanodots-driven pyroptosis suppression in nucleus pulposus for antioxidant intervention of intervertebral disc degeneration. Adv Mater 36, 2313248.","journal-title":"Adv Mater"},{"key":"2907_CR31","doi-asserted-by":"publisher","first-page":"101263","DOI":"10.1016\/j.preteyeres.2024.101263","volume":"101","author":"Y Sun","year":"2024","unstructured":"Sun, Y., Li, F., Liu, Y., Qiao, D., Yao, X., Liu, G.S., Li, D., Xiao, C., Wang, T., and Chi, W. (2024b). Targeting inflammasomes and pyroptosis in retinal diseases\u2014molecular mechanisms and future perspectives. Prog Retinal Eye Res 101, 101263.","journal-title":"Prog Retinal Eye Res"},{"key":"2907_CR32","doi-asserted-by":"publisher","first-page":"122562","DOI":"10.1016\/j.biomaterials.2024.122562","volume":"308","author":"SN Tang","year":"2024","unstructured":"Tang, S.N., Salazar-Puerta, A.I., Heimann, M.K., Kuchynsky, K., Rincon-Benavides, M.A., Kordowski, M., Gunsch, G., Bodine, L., Diop, K., Gantt, C., et al. (2024). Engineered extracellular vesicle-based gene therapy for the treatment of discogenic back pain. Biomaterials 308, 122562.","journal-title":"Biomaterials"},{"key":"2907_CR33","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1038\/s41569-023-00946-3","volume":"21","author":"S Toldo","year":"2024","unstructured":"Toldo, S., and Abbate, A. (2024). The role of the NLRP3 inflammasome and pyroptosis in cardiovascular diseases. Nat Rev Cardiol 21, 219\u2013237.","journal-title":"Nat Rev Cardiol"},{"key":"2907_CR34","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1016\/j.joca.2021.12.004","volume":"30","author":"R Tsujimoto","year":"2022","unstructured":"Tsujimoto, R., Yurube, T., Takeoka, Y., Kanda, Y., Miyazaki, K., Ohnishi, H., Kakiuchi, Y., Miyazaki, S., Zhang, Z., Takada, T., et al. (2022). Involvement of autophagy in the maintenance of rat intervertebral disc homeostasis: An in-vitro and in-vivo RNA interference study of Atg5. Osteoarthritis Cartilage 30, 481\u2013493.","journal-title":"Osteoarthritis Cartilage"},{"key":"2907_CR35","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1186\/s40824-017-0106-6","volume":"21","author":"S van Uden","year":"2017","unstructured":"van Uden, S., Silva-Correia, J., Oliveira, J.M., and Reis, R.L. (2017). Current strategies for treatment of intervertebral disc degeneration: substitution and regeneration possibilities. BioMater Res 21, 22.","journal-title":"BioMater Res"},{"key":"2907_CR36","doi-asserted-by":"publisher","first-page":"eabj3859","DOI":"10.1126\/sciimmunol.abj3859","volume":"6","author":"C Wang","year":"2021","unstructured":"Wang, C., Yang, T., Xiao, J., Xu, C., Alippe, Y., Sun, K., Kanneganti, T.D., Monahan, J. B., Abu-Amer, Y., Lieberman, J., et al. (2021). NLRP3 inflammasome activation triggers gasdermin D-independent inflammation. Sci Immunol 6, eabj3859.","journal-title":"Sci Immunol"},{"key":"2907_CR37","doi-asserted-by":"publisher","first-page":"154998","DOI":"10.1016\/j.phymed.2023.154998","volume":"119","author":"D Wang","year":"2023","unstructured":"Wang, D., Zhang, L., He, D., Zhang, Y., Bao, J., Gao, W., Cheng, W., Zhu, C., Jin, H., Zhang, W., et al. (2023a). Systemic pharmacology reveal the mechanism by which the Qiangjin Zhuanggu Qufeng mixture inhibits LPS-induced pyroptosis of rat nucleus pulposus cells. Phytomedicine 119, 154998.","journal-title":"Phytomedicine"},{"key":"2907_CR38","doi-asserted-by":"publisher","first-page":"101081","DOI":"10.1016\/j.mtbio.2024.101081","volume":"26","author":"D Wang","year":"2024","unstructured":"Wang, D., Zhang, L., He, D., Zhang, Y., Zhao, L., Miao, Z., Cheng, W., Zhu, C., Shao, Y., Ge, G., et al. (2024). A natural hydrogel complex improves intervertebral disc degeneration by correcting fatty acid metabolism and inhibiting nucleus pulposus cell pyroptosis. Mater Today Bio 26, 101081.","journal-title":"Mater Today Bio"},{"key":"2907_CR39","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1016\/j.intimp.2019.01.004","volume":"69","author":"K Wang","year":"2019","unstructured":"Wang, K., Chen, T., Ying, X., Zhang, Z., Shao, Z., Lin, J., Xu, T., Chen, Y., Wang, X., Chen, J., et al. (2019). Ligustilide alleviated IL-1 \u03b2 induced apoptosis and extracellular matrix degradation of nucleus pulposus cells and attenuates intervertebral disc degeneration in vivo. Int Immunopharmacol 69, 398\u2013407.","journal-title":"Int Immunopharmacol"},{"key":"2907_CR40","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/j.molcel.2022.12.002","volume":"83","author":"L Wang","year":"2023","unstructured":"Wang, L., Cai, J., Zhao, X., Ma, L., Zeng, P., Zhou, L., Liu, Y., Yang, S., Cai, Z., Zhang, S., et al. (2023b). Palmitoylation prevents sustained inflammation by limiting NLRP3 inflammasome activation through chaperone-mediated autophagy. Mol Cell 83, 281\u2013297.e10.","journal-title":"Mol Cell"},{"key":"2907_CR41","doi-asserted-by":"publisher","first-page":"153884","DOI":"10.1016\/j.phymed.2021.153884","volume":"95","author":"Q Wu","year":"2022","unstructured":"Wu, Q., Liu, J., Mao, Z., Tian, L., Wang, N., Wang, G., Wang, Y., and Seto, S. (2022). Ligustilide attenuates ischemic stroke injury by promoting Drp1-mediated mitochondrial fission via activation of AMPK. Phytomedicine 95, 153884.","journal-title":"Phytomedicine"},{"key":"2907_CR42","first-page":"506","volume":"33","author":"H Xiang","year":"2024","unstructured":"Xiang, H., Zhao, W., Jiang, K., He, J., Chen, L., Cui, W., and Li, Y. (2024). Progress in regulating inflammatory biomaterials for intervertebral disc regeneration. Bioact Mater 33, 506\u2013531.","journal-title":"Bioact Mater"},{"key":"2907_CR43","doi-asserted-by":"publisher","first-page":"1966","DOI":"10.1002\/ptr.6662","volume":"34","author":"Q Xie","year":"2020","unstructured":"Xie, Q., Zhang, L., Xie, L., Zheng, Y., Liu, K., Tang, H., Liao, Y., and Li, X. (2020). Z-ligustilide: a review of its pharmacokinetics and pharmacology. Phytother Res 34, 1966\u20131991.","journal-title":"Phytother Res"},{"key":"2907_CR44","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1186\/s12951-021-00991-5","volume":"19","author":"H Xing","year":"2021","unstructured":"Xing, H., Zhang, Z., Mao, Q., Wang, C., Zhou, Y., Zhou, X., Ying, L., Xu, H., Hu, S., and Zhang, N. (2021). Injectable exosome-functionalized extracellular matrix hydrogel for metabolism balance and pyroptosis regulation in intervertebral disc degeneration. J Nanobiotechnol 19, 264.","journal-title":"J Nanobiotechnol"},{"key":"2907_CR45","doi-asserted-by":"publisher","first-page":"154176","DOI":"10.1016\/j.phymed.2022.154176","volume":"102","author":"H Xu","year":"2022","unstructured":"Xu, H., Dai, Z.H., He, G.L., Cai, H.C., Chen, X.Y., Chen, Y.L., Xu, C., and Sheng, S.R. (2022). Gamma-oryzanol alleviates intervertebral disc degeneration development by intercepting the IL-1\u03b2\/NLRP3 inflammasome positive cycle. Phytomedicine 102, 154176.","journal-title":"Phytomedicine"},{"key":"2907_CR46","doi-asserted-by":"publisher","first-page":"155216","DOI":"10.1016\/j.phymed.2023.155216","volume":"123","author":"X Xu","year":"2024","unstructured":"Xu, X., Tao, N., Sun, C., Hoffman, R.D., Shi, D., Ying, Y., Dong, S., and Gao, J. (2024). Ligustilide prevents thymic immune senescence by regulating thymosin \u03b215-dependent spatial distribution of thymic epithelial cells. Phytomedicine 123, 155216.","journal-title":"Phytomedicine"},{"key":"2907_CR47","doi-asserted-by":"publisher","first-page":"109738","DOI":"10.1016\/j.biopha.2019.109738","volume":"123","author":"L Yu","year":"2020","unstructured":"Yu, L., Hao, Y., Peng, C., Zhang, P., Zhu, J., Cai, Y., and Zhu, G. (2020). Effect of Ginsenoside Rg1 on the intervertebral disc degeneration rats and the degenerative pulposus cells and its mechanism. Biomed Pharmacother 123, 109738.","journal-title":"Biomed Pharmacother"},{"key":"2907_CR48","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1038\/s41392-021-00507-5","volume":"6","author":"P Yu","year":"2021","unstructured":"Yu, P., Zhang, X., Liu, N., Tang, L., Peng, C., and Chen, X. (2021). Pyroptosis: mechanisms and diseases. Sig Transduct Target Ther 6, 128.","journal-title":"Sig Transduct Target Ther"},{"key":"2907_CR49","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1038\/s41584-022-00783-7","volume":"18","author":"U Zehra","year":"2022","unstructured":"Zehra, U., Tryfonidou, M., Iatridis, J.C., Illien-J\u00fcnger, S., Mwale, F., and Samartzis, D. (2022). Mechanisms and clinical implications of intervertebral disc calcification. Nat Rev Rheumatol 18, 352\u2013362.","journal-title":"Nat Rev Rheumatol"},{"key":"2907_CR50","doi-asserted-by":"publisher","first-page":"118993","DOI":"10.1016\/j.lfs.2020.118993","volume":"288","author":"B Zhang","year":"2022","unstructured":"Zhang, B., Wu, D., Hu, L., Cha, X., Liu, Y., Li, J., Xie, B., Li, B., and Zheng, L. (2022a). Ligustilide inhibits the proliferation of human osteoblastoma MG63 cells through the TLR4-ERK pathway. Life Sci 288, 118993.","journal-title":"Life Sci"},{"key":"2907_CR51","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1038\/s12276-022-00729-9","volume":"54","author":"W Zhang","year":"2022","unstructured":"Zhang, W., Li, G., Luo, R., Lei, J., Song, Y., Wang, B., Ma, L., Liao, Z., Ke, W., Liu, H., et al. (2022b). Cytosolic escape of mitochondrial DNA triggers cGAS-STING-NLRP3 axis-dependent nucleus pulposus cell pyroptosis. Exp Mol Med 54, 129\u2013142.","journal-title":"Exp Mol Med"},{"key":"2907_CR52","doi-asserted-by":"publisher","first-page":"117188","DOI":"10.1016\/j.jep.2023.117188","volume":"319","author":"Y Zhang","year":"2024","unstructured":"Zhang, Y., Li, D., Jia, Z., Mei, J., Wang, Y., Zhang, Y., Zhou, Q., and Xu, F. (2024). Zhizi-Chuanxiong herb pair alleviates atherosclerosis progression in ApoE\u2212\/\u2212 mice by promoting the methylation of FGFR3 to inhibit MAPK\u2019ERK-mediated apoptosis. J Ethnopharmacol 319, 117188.","journal-title":"J Ethnopharmacol"},{"key":"2907_CR53","first-page":"51","volume":"37","author":"H Zhou","year":"2024","unstructured":"Zhou, H., He, J., Liu, R., Cheng, J., Yuan, Y., Mao, W., Zhou, J., He, H., Liu, Q., Tan, W., et al. (2024). Microenvironment-responsive metal-phenolic network release platform with ROS scavenging, anti-pyroptosis, and ECM regeneration for intervertebral disc degeneration. Bioact Mater 37, 51\u201371.","journal-title":"Bioact Mater"},{"key":"2907_CR54","doi-asserted-by":"publisher","first-page":"3357","DOI":"10.1111\/jcmm.14226","volume":"23","author":"Y Zhou","year":"2019","unstructured":"Zhou, Y., Ming, J., Li, Y., Deng, M., Chen, Q., Ma, Y., Chen, Z., Zhang, Y., and Liu, S. (2019). Ligustilide attenuates nitric oxide-induced apoptosis in rat chondrocytes and cartilage degradation via inhibiting JNK and p38 MAPK pathways. J Cell Mol Medi 23, 3357\u20133368.","journal-title":"J Cell Mol Medi"},{"key":"2907_CR55","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1038\/s41392-024-01801-8","volume":"9","author":"C Zhu","year":"2024","unstructured":"Zhu, C., Xu, S., Jiang, R., Yu, Y., Bian, J., and Zou, Z. (2024). The gasdermin family: emerging therapeutic targets in diseases. Sig Transduct Target Ther 9, 87.","journal-title":"Sig Transduct Target Ther"},{"key":"2907_CR56","doi-asserted-by":"publisher","first-page":"113117","DOI":"10.1016\/j.jep.2020.113117","volume":"263","author":"L Zhu","year":"2020","unstructured":"Zhu, L., Yu, C., Zhang, X., Yu, Z., Zhan, F., Yu, X., Wang, S., He, F., Han, Y., and Zhao, H. (2020). The treatment of intervertebral disc degeneration using Traditional Chinese Medicine. J Ethnopharmacol 263, 113117.","journal-title":"J Ethnopharmacol"},{"key":"2907_CR57","doi-asserted-by":"publisher","first-page":"1988","DOI":"10.1007\/s11427-020-1909-6","volume":"64","author":"X Zong","year":"2021","unstructured":"Zong, X., Xiao, X., Jie, F., Cheng, Y., Jin, M., Yin, Y., and Wang, Y. (2021). YTHDF1 promotes NLRP3 translation to induce intestinal epithelial cell inflammatory injury during endotoxic shock. Sci China Life Sci 64, 1988\u20131991.","journal-title":"Sci China Life Sci"},{"key":"2907_CR58","doi-asserted-by":"publisher","first-page":"628","DOI":"10.1021\/acscentsci.3c01414","volume":"10","author":"J Zou","year":"2024","unstructured":"Zou, J., Qiu, Z.C., Yu, Q.Q., Wu, J.M., Wang, Y.H., Shi, K.D., Li, Y.F., He, R.R., Qin, L., Yao, X.S., et al. (2024). Discovery of a potent antiosteoporotic drug molecular scaffold derived from Angelica sinensis and its bioinspired total synthesis. ACS Cent Sci 10, 628\u2013636.","journal-title":"ACS Cent Sci"}],"container-title":["Science China Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11427-024-2907-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11427-024-2907-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11427-024-2907-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,4]],"date-time":"2025-10-04T17:02:58Z","timestamp":1759597378000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11427-024-2907-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,2]]},"references-count":58,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["2907"],"URL":"https:\/\/doi.org\/10.1007\/s11427-024-2907-7","relation":{},"ISSN":["1674-7305","1869-1889"],"issn-type":[{"value":"1674-7305","type":"print"},{"value":"1869-1889","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,2]]},"assertion":[{"value":"28 November 2024","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no conflict of interest. The ethical review for the human clinical study was approved by the Ethics Committee of the First Affiliated Hospital of Soochow University. The study adhered to the principles outlined in the Declaration of Helsinki to ensure the protection of human subjects. Informed consent was obtained from all patients involved in the research. In brief, human NP tissue samples were obtained from patients with varying degrees of IVDD who underwent foraminal endoscopic surgery, and used for subsequent tissue staining. All procedures involving the rats were conducted in accordance with the standard guidelines approved by Soochow University\u2019s Animal Ethics Committee (Approval No. SUDA20240711A06).","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance and ethics"}}]}}