{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T03:21:54Z","timestamp":1792034514030,"version":"4.4.0"},"reference-count":69,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Sci. China Life Sci."],"published-print":{"date-parts":[[2026,3]]},"DOI":"10.1007\/s11427-024-2996-1","type":"journal-article","created":{"date-parts":[[2025,11,24]],"date-time":"2025-11-24T04:44:03Z","timestamp":1763959443000},"page":"937-953","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Single-nucleus RNA sequencing reveals the underlying roadmap of early gonadal differentiation in teleost"],"prefix":"10.1007","volume":"69","author":[{"given":"Jialin","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajuan","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongkai","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaili","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bowen","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yadong","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Schartl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songlin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,11,20]]},"reference":[{"key":"2996_CR1","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1093\/molehr\/gay009","volume":"24","author":"S Bai","year":"2018","unstructured":"Bai, S., Cheng, L., Zhang, Y., Zhu, C., Zhu, Z., Zhu, R., Cheng, C.Y., Ye, L., and Zheng, K. (2018). A germline-specific role for the mTORC2 component Rictor in maintaining spermatogonial differentiation and intercellular adhesion in mouse testis. Mol Hum Reprod 24, 244\u2013259.","journal-title":"Mol Hum Reprod"},{"key":"2996_CR2","doi-asserted-by":"publisher","first-page":"e26950","DOI":"10.1371\/journal.pone.0026950","volume":"6","author":"A Baillet","year":"2011","unstructured":"Baillet, A., Le Bouffant, R., Volff, J.N., Luangpraseuth, A., Poumerol, E., Th\u00e9pot, D., Pailhoux, E., Livera, G., Cotinot, C., Mandon-P\u00e9pin, B., et al. (2011). TOPAZ1, a novel germ cell-specific expressed gene conserved during evolution across vertebrates. PLoS One 6, e26950.","journal-title":"PLoS One"},{"key":"2996_CR3","doi-asserted-by":"publisher","first-page":"308","DOI":"10.1016\/j.ydbio.2012.12.003","volume":"374","author":"RL Beer","year":"2013","unstructured":"Beer, R.L., and Draper, B.W. (2013). nanos3 maintains germline stem cells and expression of the conserved germline stem cell gene nanos2 in the zebrafish ovary. Dev Biol 374, 308\u2013318.","journal-title":"Dev Biol"},{"key":"2996_CR4","doi-asserted-by":"publisher","first-page":"1202693","DOI":"10.3389\/fcell.2023.1202693","volume":"11","author":"S Biswas","year":"2023","unstructured":"Biswas, S., Ghosh, S., and Maitra, S. (2023). Role of insulin-like growth factor 1 (IGF1) in the regulation of mitochondrial bioenergetics in zebrafish oocytes: lessons from in vivo and in vitro investigations. Front Cell Dev Biol 11, 1202693.","journal-title":"Front Cell Dev Biol"},{"key":"2996_CR5","doi-asserted-by":"publisher","first-page":"14994","DOI":"10.1073\/pnas.0701677104","volume":"104","author":"GJ Bouma","year":"2007","unstructured":"Bouma, G.J., Washburn, L.L., Albrecht, K.H., and Eicher, E.M. (2007). Correct dosage of Fog2 and Gata4 transcription factors is critical for fetal testis development in mice. Proc Natl Acad Sci USA 104, 14994\u201314999.","journal-title":"Proc Natl Acad Sci USA"},{"key":"2996_CR6","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1007\/s12079-021-00628-0","volume":"16","author":"Y Cao","year":"2022","unstructured":"Cao, Y., Liu, L., Lin, J., Sun, P., Guo, K., Li, S., Li, X., Lan, Z., Wang, H., and Lei, Z. (2022). Dysregulation of Notch-FGF signaling axis in germ cells results in cystic dilation of the rete testis in mice. J Cell Commun Signal 16, 75\u201392.","journal-title":"J Cell Commun Signal"},{"key":"2996_CR7","doi-asserted-by":"publisher","first-page":"1709","DOI":"10.1016\/j.celrep.2019.01.061","volume":"26","author":"Z Cao","year":"2019","unstructured":"Cao, Z., Mao, X., and Luo, L. (2019). Germline stem cells drive ovary regeneration in zebrafish. Cell Rep 26, 1709\u20131717.e3.","journal-title":"Cell Rep"},{"key":"2996_CR8","doi-asserted-by":"publisher","first-page":"4461","DOI":"10.1242\/dev.078972","volume":"139","author":"AA Chassot","year":"2012","unstructured":"Chassot, A.A., Bradford, S.T., Auguste, A., Gregoire, E.P., Pailhoux, E., de Rooij, D.G., Schedl, A., and Chaboissier, M.C. (2012). WNT4 and RSPO1 together are required for cell proliferation in the early mouse gonad. Development 139, 4461\u20134472.","journal-title":"Development"},{"key":"2996_CR9","doi-asserted-by":"publisher","first-page":"e1009778","DOI":"10.1371\/journal.pgen.1009778","volume":"17","author":"B Chen","year":"2021","unstructured":"Chen, B., Zhu, G., Yan, A., He, J., Liu, Y., Li, L., Yang, X., Dong, C., Kee, K., and Hammoud, S. (2021). IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene. PLoS Genet 17, e1009778.","journal-title":"PLoS Genet"},{"key":"2996_CR10","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.cbpb.2015.01.002","volume":"183","author":"H Chen","year":"2015","unstructured":"Chen, H., Li, S., Xiao, L., Zhang, Y., Li, G., Liu, X., and Lin, H. (2015). Wnt4 in protogynous hermaphroditic orange-spotted grouper (Epinephelus coioides): identification and expression. Comp Biochem Physiol Part B 183, 67\u201374.","journal-title":"Comp Biochem Physiol Part B"},{"key":"2996_CR11","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1038\/ng.2890","volume":"46","author":"S Chen","year":"2014","unstructured":"Chen, S., Zhang, G., Shao, C., Huang, Q., Liu, G., Zhang, P., Song, W., An, N., Chalopin, D., Volff, J.N., et al. (2014). Whole-genome sequence of a flatfish provides insights into ZW sex chromosome evolution and adaptation to a benthic lifestyle. Nat Genet 46, 253\u2013260.","journal-title":"Nat Genet"},{"key":"2996_CR12","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1007\/s10126-011-9395-2","volume":"14","author":"SL Chen","year":"2012","unstructured":"Chen, S.L., Ji, X.S., Shao, C.W., Li, W.L., Yang, J.F., Liang, Z., Liao, X.L., Xu, G.B., Xu, Y., and Song, W.T. (2012). Induction of mitogynogenetic diploids and identification of WW super-female using sex-specific SSR markers in half-smooth tongue sole (Cynoglossus semilaevis). Mar Biotechnol 14, 120\u2013128.","journal-title":"Mar Biotechnol"},{"key":"2996_CR13","doi-asserted-by":"publisher","first-page":"42213","DOI":"10.1038\/srep42213","volume":"7","author":"Z Cui","year":"2017","unstructured":"Cui, Z., Liu, Y., Wang, W., Wang, Q., Zhang, N., Lin, F., Wang, N., Shao, C., Dong, Z., Li, Y., et al. (2017). Genome editing reveals dmrt1 as an essential male sex-determining gene in Chinese tongue sole (Cynoglossus semilaevis). Sci Rep 7, 42213.","journal-title":"Sci Rep"},{"key":"2996_CR14","doi-asserted-by":"publisher","first-page":"4244","DOI":"10.1210\/en.2015-1217","volume":"156","author":"H Dong","year":"2015","unstructured":"Dong, H., Chen, Z., Wang, C., Xiong, Z., Zhao, W., Jia, C., Lin, J., Lin, Y., Yuan, W., Zhao, A.Z., et al. (2015). Rictor regulates spermatogenesis by controlling sertoli cell cytoskeletal organization and cell polarity in the mouse testis. Endocrinology 156, 4244\u20134256.","journal-title":"Endocrinology"},{"key":"2996_CR15","doi-asserted-by":"publisher","first-page":"1484","DOI":"10.1038\/s41596-020-0292-x","volume":"15","author":"M Efremova","year":"2020","unstructured":"Efremova, M., Vento-Tormo, M., Teichmann, S.A., and Vento-Tormo, R. (2020). CellPhoneDB: inferring cell-cell communication from combined expression of multi-subunit ligand-receptor complexes. Nat Protoc 15, 1484\u20131506.","journal-title":"Nat Protoc"},{"key":"2996_CR16","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1186\/1477-7827-10-108","volume":"10","author":"A Evron","year":"2012","unstructured":"Evron, A., Goldman, S., and Shalev, E. (2012). Human amniotic epithelial cells differentiate into cells expressing germ cell specific markers when cultured in medium containing serum substitute supplement. Reprod Biol Endocrinol 10, 108.","journal-title":"Reprod Biol Endocrinol"},{"key":"2996_CR17","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1016\/j.ydbio.2019.11.008","volume":"459","author":"DR Farnsworth","year":"2020","unstructured":"Farnsworth, D.R., Saunders, L.M., and Miller, A.C. (2020). A single-cell transcriptome atlas for zebrafish development. Dev Biol 459, 100\u2013108.","journal-title":"Dev Biol"},{"key":"2996_CR18","doi-asserted-by":"publisher","first-page":"pdb.prot4986","DOI":"10.1101\/pdb.prot4986","volume":"5","author":"AH Fischer","year":"2008","unstructured":"Fischer, A.H., Jacobson, K.A., Rose, J., and Zeller, R. (2008). Hematoxylin and eosin staining of tissue and cell sections. Cold Spring Harb Protoc 5, pdb.prot4986.","journal-title":"Cold Spring Harb Protoc"},{"key":"2996_CR19","doi-asserted-by":"publisher","first-page":"1415","DOI":"10.1021\/ac302330a","volume":"85","author":"JM Fonville","year":"2013","unstructured":"Fonville, J.M., Carter, C.L., Pizarro, L., Steven, R.T., Palmer, A.D., Griffiths, R.L., Lalor, P.F., Lindon, J.C., Nicholson, J.K., Holmes, E., et al. (2013). Hyperspectral visualization of mass spectrometry imaging data. Anal Chem 85, 1415\u20131423.","journal-title":"Anal Chem"},{"key":"2996_CR20","doi-asserted-by":"publisher","first-page":"3385","DOI":"10.7150\/thno.94943","volume":"14","author":"Y Fu","year":"2024","unstructured":"Fu, Y., Zhang, M., Sui, B., Yuan, F.F., Zhang, W., Weng, Y., Xiang, L., Li, C., Shao, L., You, Y., et al. (2024). Mesenchymal stem cell-derived apoptotic vesicles ameliorate impaired ovarian folliculogenesis in polycystic ovary syndrome and ovarian aging by targeting WNT signaling. Theranostics 14, 3385\u20133403.","journal-title":"Theranostics"},{"key":"2996_CR21","doi-asserted-by":"publisher","first-page":"416","DOI":"10.1016\/j.devcel.2013.06.018","volume":"26","author":"Y Fujimoto","year":"2013","unstructured":"Fujimoto, Y., Tanaka, S.S., Yamaguchi, Y.L., Kobayashi, H., Kuroki, S., Tachibana, M., Shinomura, M., Kanai, Y., Morohashi, K., Kawakami, K., et al. (2013). Homeoproteins Six1 and Six4 regulate male sex determination and mouse gonadal development. Dev Cell 26, 416\u2013430.","journal-title":"Dev Cell"},{"key":"2996_CR22","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/1471-2105-14-7","volume":"14","author":"S H\u00e4nzelmann","year":"2013","unstructured":"H\u00e4nzelmann, S., Castelo, R., and Guinney, J. (2013). GSVA: gene set variation analysis for microarray and RNA-Seq data. BMC BioInf 14, 7.","journal-title":"BMC BioInf"},{"key":"2996_CR23","doi-asserted-by":"publisher","first-page":"3573","DOI":"10.1016\/j.cell.2021.04.048","volume":"184","author":"Y Hao","year":"2021","unstructured":"Hao, Y., Hao, S., Andersen-Nissen, E., Mauck Iii, W.M., Zheng, S., Butler, A., Lee, M.J., Wilk, A.J., Darby, C., Zager, M., et al. (2021). Integrated analysis of multimodal single-cell data. Cell 184, 3573\u20133587.e29.","journal-title":"Cell"},{"key":"2996_CR24","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1095\/biolreprod.111.091629","volume":"85","author":"DE Hickford","year":"2011","unstructured":"Hickford, D.E., Frankenberg, S., Pask, A.J., Shaw, G., and Renfree, M.B. (2011). DDX4 (VASA) is conserved in germ cell development in marsupials and monotremes1. Biol Reprod 85, 733\u2013743.","journal-title":"Biol Reprod"},{"key":"2996_CR25","doi-asserted-by":"publisher","first-page":"2528","DOI":"10.1016\/j.yexcr.2006.04.015","volume":"312","author":"T Iwai","year":"2006","unstructured":"Iwai, T., Yoshii, A., Yokota, T., Sakai, C., Hori, H., Kanamori, A., and Yamashita, M. (2006). Structural components of the synaptonemal complex, SYCP1 and SYCP3, in the medaka fish Oryzias latipes. Exp Cell Res 312, 2528\u20132537.","journal-title":"Exp Cell Res"},{"key":"2996_CR26","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1530\/eje.0.1470397","volume":"147","author":"I Ketola","year":"2002","unstructured":"Ketola, I., Anttonen, M., Vaskivuo, T., Tapanainen, J.S., Toppari, J., and Heikinheimo, M. (2002). Developmental expression and spermatogenic stage specificity of transcription factors GATA-1 and GATA-4 and their cofactors FOG-1 and FOG-2 in the mouse testis. Eur J Endocrinol 147, 397\u2013406.","journal-title":"Eur J Endocrinol"},{"key":"2996_CR27","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1186\/1471-2105-9-559","volume":"9","author":"P Langfelder","year":"2008","unstructured":"Langfelder, P., and Horvath, S. (2008). WGCNA: an R package for weighted correlation network analysis. BMC BioInf 9, 559.","journal-title":"BMC BioInf"},{"key":"2996_CR28","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1128\/MCB.24.1.377-388.2004","volume":"24","author":"N Lei","year":"2004","unstructured":"Lei, N., and Heckert, L.L. (2004). Gata4 regulates testis expression of Dmrt1. Mol Cell Biol 24, 377\u2013388.","journal-title":"Mol Cell Biol"},{"key":"2996_CR29","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1111\/rda.13920","volume":"56","author":"Z Li","year":"2021","unstructured":"Li, Z., Wang, J., Zhao, Y., Ma, D., Zhao, M., Li, N., Men, Y., Zhang, Y., Chu, H., Lei, C., et al. (2021). scRNA-seq of ovarian follicle granulosa cells from different fertility goats reveals distinct expression patterns. Reprod Domest Anim 56, 801\u2013811.","journal-title":"Reprod Domest Anim"},{"key":"2996_CR30","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1016\/j.cels.2015.12.004","volume":"1","author":"A Liberzon","year":"2015","unstructured":"Liberzon, A., Birger, C., Thorvaldsd\u00f3ttir, H., Ghandi, M., Mesirov, J.P., and Tamayo, P. (2015). The molecular signatures database hallmark gene set collection. Cell Syst 1, 417\u2013425.","journal-title":"Cell Syst"},{"key":"2996_CR31","doi-asserted-by":"publisher","first-page":"110110","DOI":"10.1016\/j.celrep.2021.110110","volume":"37","author":"R Liu","year":"2021","unstructured":"Liu, R., Kasowitz, S.D., Homolka, D., Leu, N.A., Shaked, J.T., Ruthel, G., Jain, D., Lin, H., Keeney, S., Luo, M., et al. (2021). YTHDC2 is essential for pachytene progression and prevents aberrant microtubule-driven telomere clustering in male meiosis. Cell Rep 37, 110110.","journal-title":"Cell Rep"},{"key":"2996_CR32","doi-asserted-by":"publisher","first-page":"828124","DOI":"10.3389\/fcell.2022.828124","volume":"10","author":"X Liu","year":"2022","unstructured":"Liu, X., Huang, Y., Tan, F., Wang, H.Y., Chen, J.Y., Zhang, X., Zhao, X., Liu, K., Wang, Q., Liu, S., et al. (2022a). Single-cell atlas of the Chinese tongue sole (Cynoglossus semilaevis) ovary reveals transcriptional programs of oogenesis in fish. Front Cell Dev Biol 10, 828124.","journal-title":"Front Cell Dev Biol"},{"key":"2996_CR33","doi-asserted-by":"publisher","first-page":"e76014","DOI":"10.7554\/eLife.76014","volume":"11","author":"Y Liu","year":"2022","unstructured":"Liu, Y., Kossack, M.E., McFaul, M.E., Christensen, L.N., Siebert, S., Wyatt, S.R., Kamei, C.N., Horst, S., Arroyo, N., Drummond, I.A., et al. (2022b). Single-cell transcriptome reveals insights into the development and function of the zebrafish ovary. eLife 11, e76014.","journal-title":"eLife"},{"key":"2996_CR34","first-page":"55","volume":"2","author":"X Ma","year":"2006","unstructured":"Ma, X., Liu, X., Wen, H., Xu, Y., and Zhang, L. (2006). Histological observation on gonadal sex differentiation in Cynoglossus semilaevis (in Chinese). Prog Fish Sci 2, 55\u201361.","journal-title":"Prog Fish Sci"},{"key":"2996_CR35","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1038\/s42003-024-06418-9","volume":"7","author":"JF Madsen","year":"2024","unstructured":"Madsen, J.F., Ernst, E.H., Amoushahi, M., Dueholm, M., Ernst, E., and Lykke-Hartmann, K. (2024). Dorsomorphin inhibits AMPK, upregulates Wnt and Foxo genes and promotes the activation of dormant follicles. Commun Biol 7, 747.","journal-title":"Commun Biol"},{"key":"2996_CR36","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1038\/nature10239","volume":"476","author":"CK Matson","year":"2011","unstructured":"Matson, C.K., Murphy, M.W., Sarver, A.L., Griswold, M.D., Bardwell, V.J., and Zarkower, D. (2011). DMRT1 prevents female reprogramming in the postnatal mammalian testis. Nature 476, 101\u2013104.","journal-title":"Nature"},{"key":"2996_CR37","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1038\/nrg3161","volume":"13","author":"CK Matson","year":"2012","unstructured":"Matson, C.K., and Zarkower, D. (2012). Sex and the singular DM domain: insights into sexual regulation, evolution and plasticity. Nat Rev Genet 13, 163\u2013174.","journal-title":"Nat Rev Genet"},{"key":"2996_CR38","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1007\/s10695-023-01200-w","volume":"49","author":"OD Moreno Acosta","year":"2023","unstructured":"Moreno Acosta, O.D., Boan, A.F., Hattori, R.S., and Fernandino, J.I. (2023). Notch pathway is required for protection against heat stress in spermatogonial stem cells in medaka. Fish Physiol Biochem 49, 487\u2013500.","journal-title":"Fish Physiol Biochem"},{"key":"2996_CR39","doi-asserted-by":"publisher","first-page":"5170","DOI":"10.1016\/j.ygeno.2020.09.031","volume":"112","author":"J Niu","year":"2020","unstructured":"Niu, J., Huang, Y., Liu, X., Zhang, Z., Tang, J., Wang, B., Lu, Y., Cai, J., and Jian, J. (2020). Single-cell RNA-seq reveals different subsets of non-specific cytotoxic cells in teleost. Genomics 112, 5170\u20135179.","journal-title":"Genomics"},{"key":"2996_CR40","doi-asserted-by":"publisher","first-page":"e1011210","DOI":"10.1371\/journal.pgen.1011210","volume":"20","author":"S Qi","year":"2024","unstructured":"Qi, S., Dai, S., Zhou, X., Wei, X., Chen, P., He, Y., Kocher, T.D., Wang, D., Li, M., and Schartl, M. (2024). Dmrt1 is the only male pathway gene tested indispensable for sex determination and functional testis development in tilapia. PLoS Genet 20, e1011210.","journal-title":"PLoS Genet"},{"key":"2996_CR41","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1038\/nmeth.4402","volume":"14","author":"X Qiu","year":"2017","unstructured":"Qiu, X., Mao, Q., Tang, Y., Wang, L., Chawla, R., Pliner, H.A., and Trapnell, C. (2017). Reversed graph embedding resolves complex single-cell trajectories. Nat Methods 14, 979\u2013982.","journal-title":"Nat Methods"},{"key":"2996_CR42","doi-asserted-by":"publisher","first-page":"1116","DOI":"10.24272\/j.issn.2095-8137.2024.046","volume":"45","author":"Z Ren","year":"2024","unstructured":"Ren, Z., Ye, D., Su, N., Wang, C., He, L., Wang, H., He, M., and Sun, Y. (2024). foxl2l is a germ cell-intrinsic gatekeeper of oogenesis in zebrafish. Zool Res 45, 1116\u20131130.","journal-title":"Zool Res"},{"key":"2996_CR43","doi-asserted-by":"publisher","first-page":"3252","DOI":"10.1016\/j.celrep.2018.11.090","volume":"25","author":"JM Saju","year":"2018","unstructured":"Saju, J.M., Hossain, M.S., Liew, W.C., Pradhan, A., Thevasagayam, N.M., Tan, L.S.E., Anand, A., Olsson, P.E., and Orb\u00e1n, L. (2018). Heat shock factor 5 is essential for spermatogenesis in zebrafish. Cell Rep 25, 3252\u20133261.e4.","journal-title":"Cell Rep"},{"key":"2996_CR44","doi-asserted-by":"publisher","first-page":"2498","DOI":"10.1101\/gr.1239303","volume":"13","author":"P Shannon","year":"2003","unstructured":"Shannon, P., Markiel, A., Ozier, O., Baliga, N.S., Wang, J.T., Ramage, D., Amin, N., Schwikowski, B., and Ideker, T. (2003). Cytoscape: a software environment for integrated models of biomolecular interaction networks. Genome Res 13, 2498\u20132504.","journal-title":"Genome Res"},{"key":"2996_CR45","doi-asserted-by":"publisher","first-page":"580","DOI":"10.1095\/biolreprod.108.074971","volume":"82","author":"A Sironen","year":"2010","unstructured":"Sironen, A., Hansen, J., Thomsen, B., Andersson, M., Vilkki, J., Toppari, J., and Kotaja, N. (2010). Expression of SPEF2 during mouse spermatogenesis and identification of IFT20 as an interacting protein1. Biol Reprod 82, 580\u2013590.","journal-title":"Biol Reprod"},{"key":"2996_CR46","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1038\/nature08298","volume":"461","author":"CA Smith","year":"2009","unstructured":"Smith, C.A., Roeszler, K.N., Ohnesorg, T., Cummins, D.M., Farlie, P.G., Doran, T.J., and Sinclair, A.H. (2009). The avian Z-linked gene DMRT1 is required for male sex determination in the chicken. Nature 461, 267\u2013271.","journal-title":"Nature"},{"key":"2996_CR47","volume-title":"Gastrulation: From Cells to Embryo","author":"CD Stern","year":"2004","unstructured":"Stern, C.D. (2004). Gastrulation: From Cells to Embryo (New York: Cold Spring Harbor Laboratory Press)."},{"key":"2996_CR48","doi-asserted-by":"publisher","first-page":"3272","DOI":"10.1016\/j.celrep.2019.02.069","volume":"26","author":"I St\u00e9vant","year":"2019","unstructured":"St\u00e9vant, I., K\u00fchne, F., Greenfield, A., Chaboissier, M.C., Dermitzakis, E.T., and Nef, S. (2019). Dissecting cell lineage specification and sex fate determination in gonadal somatic cells using single-cell transcriptomics. Cell Rep 26, 3272\u20133283.e3.","journal-title":"Cell Rep"},{"key":"2996_CR49","doi-asserted-by":"publisher","first-page":"1888","DOI":"10.1016\/j.cell.2019.05.031","volume":"177","author":"T Stuart","year":"2019","unstructured":"Stuart, T., Butler, A., Hoffman, P., Hafemeister, C., Papalexi, E., Mauck Iii, W.M., Hao, Y., Stoeckius, M., Smibert, P., and Satija, R. (2019). Comprehensive integration of single-cell data. Cell 177, 1888\u20131902.e21.","journal-title":"Cell"},{"key":"2996_CR50","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1038\/s41586-018-0698-6","volume":"563","author":"R Vento-Tormo","year":"2018","unstructured":"Vento-Tormo, R., Efremova, M., Botting, R.A., Turco, M.Y., Vento-Tormo, M., Meyer, K.B., Park, J.E., Stephenson, E., Pola\u0144ski, K., Goncalves, A., et al. (2018). Single-cell reconstruction of the early maternal-fetal interface in humans. Nature 563, 347\u2013353.","journal-title":"Nature"},{"key":"2996_CR51","first-page":"91","volume":"15","author":"J Wan","year":"2024","unstructured":"Wan, J., Jiang, Y., and Zhuang, Z. (2024). Morphological and developmental characters at the early stages of the tonguefish Cynoglossus semilaevis. Curr Zool 15, 91\u2013102.","journal-title":"Curr Zool"},{"key":"2996_CR52","doi-asserted-by":"publisher","first-page":"1151","DOI":"10.1007\/s11427-021-2236-4","volume":"66","author":"HY Wang","year":"2023","unstructured":"Wang, H.Y., Liu, X., Chen, J.Y., Huang, Y., Lu, Y., Tan, F., Liu, Q., Yang, M., Li, S., Zhang, X., et al. (2023a). Single-cell-resolution transcriptome map revealed novel genes involved in testicular germ cell progression and somatic cells specification in Chinese tongue sole with sex reversal. Sci China Life Sci 66, 1151\u20131169.","journal-title":"Sci China Life Sci"},{"key":"2996_CR53","doi-asserted-by":"publisher","first-page":"147089","DOI":"10.1016\/j.gene.2022.147089","volume":"853","author":"J Wang","year":"2023","unstructured":"Wang, J., Shi, R., Yang, Q., Chen, Z., Wang, J., Gong, Z., Chen, S., and Wang, N. (2023b). Characterization and potential function of 7-dehydrocholesterol reductase (dhcr7) and lathosterol 5-desaturase (sc5d) in Cynoglossus semilaevis sexual size dimorphism. Gene 853, 147089.","journal-title":"Gene"},{"key":"2996_CR54","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1007\/s10142-018-0595-y","volume":"18","author":"N Wang","year":"2018","unstructured":"Wang, N., Wang, R., Wang, R., and Chen, S. (2018). Transcriptomics analysis revealing candidate networks and genes for the body size sexual dimorphism of Chinese tongue sole (Cynoglossus semilaevis). Funct Integr Genomic 18, 327\u2013339.","journal-title":"Funct Integr Genomic"},{"key":"2996_CR55","doi-asserted-by":"publisher","first-page":"e1009665","DOI":"10.1371\/journal.ppat.1009665","volume":"17","author":"Q Wang","year":"2021","unstructured":"Wang, Q., Peng, C., Yang, M., Huang, F., Duan, X., Wang, S., Cheng, H., Yang, H., Zhao, H., Qin, Q., et al. (2021). Single-cell RNA-seq landscape midbrain cell responses to red spotted grouper nervous necrosis virus infection. PLoS Pathog 17, e1009665.","journal-title":"PLoS Pathog"},{"key":"2996_CR56","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1186\/s13059-017-1382-0","volume":"19","author":"FA Wolf","year":"2018","unstructured":"Wolf, F.A., Angerer, P., and Theis, F.J. (2018). SCANPY: large-scale single-cell gene expression data analysis. Genome Biol 19, 15.","journal-title":"Genome Biol"},{"key":"2996_CR57","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1186\/s13059-019-1663-x","volume":"20","author":"FA Wolf","year":"2019","unstructured":"Wolf, F.A., Hamey, F.K., Plass, M., Solana, J., Dahlin, J.S., G\u00f6ttgens, B., Rajewsky, N., Simon, L., and Theis, F.J. (2019). PAGA: graph abstraction reconciles clustering with trajectory inference through a topology preserving map of single cells. Genome Biol 20, 59.","journal-title":"Genome Biol"},{"key":"2996_CR58","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/j.ygcen.2019.03.016","volume":"277","author":"L Wu","year":"2019","unstructured":"Wu, L., Li, Y., Xu, Y., Li, Y., Wang, L., Ma, X., Liu, H., Li, X., and Zhou, L. (2019). Cloning and characterization of wnt4a gene in a natural triploid teleost, Qi river crucian carp (Carassius auratus). Gen Comp Endocrinol 277, 104\u2013111.","journal-title":"Gen Comp Endocrinol"},{"key":"2996_CR59","doi-asserted-by":"publisher","first-page":"e2018345118","DOI":"10.1073\/pnas.2018345118","volume":"118","author":"G Xi","year":"2021","unstructured":"Xi, G., An, L., Wang, W., Hao, J., Yang, Q., Ma, L., Lu, J., Wang, Y., Wang, W., Zhao, W., et al. (2021). The mRNA-destabilizing protein Tristetraprolin targets \u201cmeiosis arrester\u201d Nppc mRNA in mammalian preovulatory follicles. Proc Natl Acad Sci USA 118, e2018345118.","journal-title":"Proc Natl Acad Sci USA"},{"key":"2996_CR60","doi-asserted-by":"publisher","first-page":"100919","DOI":"10.1016\/j.cbd.2021.100919","volume":"40","author":"W Xu","year":"2021","unstructured":"Xu, W., Cui, Z., Wang, N., Zhang, M., Wang, J., Xu, X., Liu, Y., and Chen, S. (2021). Transcriptomic analysis revealed gene expression profiles during the sex differentiation of Chinese tongue sole (Cynoglossus semilaevis). Comp Biochem Physiol D Genomics Proteomics 40, 100919.","journal-title":"Comp Biochem Physiol D Genomics Proteomics"},{"key":"2996_CR61","doi-asserted-by":"publisher","first-page":"737956","DOI":"10.1016\/j.aquaculture.2022.737956","volume":"551","author":"Q Yang","year":"2022","unstructured":"Yang, Q., Li, X., Shi, R., Cheng, P., Wang, N., and Chen, S. (2022). The female-biased expression, transcriptional regulation and knock-down effect of insulin-like growth factor binding protein 7 in Chinese tongue sole, Cynoglossus semilaevis. Aquaculture 551, 737956.","journal-title":"Aquaculture"},{"key":"2996_CR62","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1095\/biolreprod55.1.152","volume":"55","author":"Y Yoshimura","year":"1996","unstructured":"Yoshimura, Y., Ando, M., Nagamatsu, S., Iwashita, M., Adachi, T., Sueoka, K., Miyazaki, T., Kuji, N., and Tanaka, M. (1996). Effects of insulin-like growth factor-I on follicle growth, oocyte maturation, and ovarian steroidogenesis and plasminogen activator activity in the rabbit. Biol Reprod 55, 152\u2013160.","journal-title":"Biol Reprod"},{"key":"2996_CR63","doi-asserted-by":"publisher","first-page":"732","DOI":"10.1038\/s41586-022-05526-y","volume":"612","author":"J Zhai","year":"2022","unstructured":"Zhai, J., Guo, J., Wan, H., Qi, L., Liu, L., Xiao, Z., Yan, L., Schmitz, D.A., Xu, Y., Yu, D., et al. (2022). Primate gastrulation and early organogenesis at single-cell resolution. Nature 612, 732\u2013738.","journal-title":"Nature"},{"key":"2996_CR64","doi-asserted-by":"publisher","first-page":"1459427","DOI":"10.3389\/fgene.2024.1459427","volume":"15","author":"D Zhang","year":"2024","unstructured":"Zhang, D., Tian, T., Li, S., Du, J., Lei, C., Zhu, T., Han, L., and Song, H. (2024a). Transcriptome analysis of four types of gonadal tissues in largemouth bass (Micropterus salmoides) to reveal its sex-related genes. Front Genet 15, 1459427.","journal-title":"Front Genet"},{"key":"2996_CR65","doi-asserted-by":"publisher","first-page":"D144","DOI":"10.1093\/nar\/gkr965","volume":"40","author":"HM Zhang","year":"2012","unstructured":"Zhang, H.M., Chen, H., Liu, W., Liu, H., Gong, J., Wang, H., and Guo, A.Y. (2012). AnimalTFDB: a comprehensive animal transcription factor database. Nucleic Acids Res 40, D144\u2013D149.","journal-title":"Nucleic Acids Res"},{"key":"2996_CR66","doi-asserted-by":"publisher","first-page":"2203631","DOI":"10.1002\/advs.202203631","volume":"9","author":"R Zhang","year":"2022","unstructured":"Zhang, R., Tu, Y., Ye, D., Gu, Z., Chen, Z., and Sun, Y. (2022). A germline-specific regulator of mitochondrial fusion is required for maintenance and differentiation of germline stem and progenitor cells. Adv Sci 9, 2203631.","journal-title":"Adv Sci"},{"key":"2996_CR67","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1186\/s13293-024-00643-x","volume":"15","author":"X Zhang","year":"2024","unstructured":"Zhang, X., Wu, Y., Zhang, Y., Zhang, J., Chu, P., Chen, K., Liu, H., Luo, Q., Fei, S., Zhao, J., et al. (2024b). Histological observations and transcriptome analyses reveal the dynamic changes in the gonads of the blotched snakehead (Channa maculata) during sex differentiation and gametogenesis. Biol Sex Differ 15, 70.","journal-title":"Biol Sex Differ"},{"key":"2996_CR68","doi-asserted-by":"publisher","first-page":"2064","DOI":"10.1007\/s11427-023-2670-5","volume":"67","author":"Q Zhou","year":"2024","unstructured":"Zhou, Q., Wang, J., Li, J., Chen, Z., Wang, N., Li, M., Wang, L., Si, Y., Lu, S., Cui, Z., et al. (2024). Decoding the fish genome opens a new era in important trait research and molecular breeding in China. Sci China Life Sci 67, 2064\u20132083.","journal-title":"Sci China Life Sci"},{"key":"2996_CR69","unstructured":"Zhuang, Z., Wan, J., Chen, S., and Liu, X. (2005). Feeding and growth of the tonguefish Cynoglossus semilaevis larvae. Current Zoology 51."}],"container-title":["Science China Life Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11427-024-2996-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11427-024-2996-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11427-024-2996-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T10:02:40Z","timestamp":1772791360000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11427-024-2996-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,20]]},"references-count":69,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["2996"],"URL":"https:\/\/doi.org\/10.1007\/s11427-024-2996-1","relation":{},"ISSN":["1674-7305","1869-1889"],"issn-type":[{"value":"1674-7305","type":"print"},{"value":"1869-1889","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,20]]},"assertion":[{"value":"20 March 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 November 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no conflict of interest. This study was conducted by the principles of the Animal Care and Use Committee at the Chinese Academy of Fishery Sciences. During all the experimental processes, we fully complied with the animal welfare ethics and animal experiment safety guidelines of the Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences. The corresponding animal ethics approval number is YSFRI-2025017.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance and ethics"}}]}}