{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T20:12:58Z","timestamp":1793304778878,"version":"4.5.0"},"reference-count":68,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T00:00:00Z","timestamp":1610582400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T00:00:00Z","timestamp":1610582400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["In Vitro Cell.Dev.Biol.-Animal"],"published-print":{"date-parts":[[2021,2]]},"DOI":"10.1007\/s11626-020-00539-1","type":"journal-article","created":{"date-parts":[[2021,1,14]],"date-time":"2021-01-14T12:03:25Z","timestamp":1610625805000},"page":"148-159","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["In vitro three-dimensional organotypic culture models of the oral mucosa"],"prefix":"10.1007","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0651-6895","authenticated-orcid":false,"given":"Mitchell","family":"Klausner","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Handa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiya","family":"Aizawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,1,14]]},"reference":[{"key":"539_CR1","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1007\/s40268-017-0212-x","volume":"17","author":"A Battaglia","year":"2017","unstructured":"Battaglia A, Nguyen T (2017) Transmucosal delivery of nicotine in combination with tincture of benzoin inhibits apoptosis. Drugs R D 17:615\u2013621","journal-title":"Drugs R D"},{"issue":"7","key":"539_CR2","doi-asserted-by":"publisher","first-page":"704","DOI":"10.1080\/21505594.2015.1081731","volume":"6","author":"GN Belibasakis","year":"2015","unstructured":"Belibasakis GN, Kast JI, Thurnheer T, Akdis CA, Bostanci N (2015) The expression of gingival epithelial junctions in response to subgingival biofilms. Virulence. 6(7):704\u2013709","journal-title":"Virulence."},{"issue":"1","key":"539_CR3","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1186\/2045-8118-10-5","volume":"10","author":"K Benson","year":"2013","unstructured":"Benson K, Cramer S, Galla HJ (2013) Impedance-based cell monitoring: barrier properties and beyond. Fluids Barriers CNS 10(1):5","journal-title":"Fluids Barriers CNS"},{"issue":"3","key":"539_CR4","doi-asserted-by":"publisher","first-page":"1479568","DOI":"10.1080\/21688370.2018.1479568","volume":"6","author":"L Bierbaumer","year":"2018","unstructured":"Bierbaumer L, Schwarze UY, Gruber R, Neuhaus W (2018) Cell culture models of oral mucosal barriers: a review with a focus on applications, culture conditions and barrier properties. Tissue Barriers 6(3):1479568","journal-title":"Tissue Barriers"},{"issue":"11","key":"539_CR5","doi-asserted-by":"publisher","first-page":"1163","DOI":"10.2174\/0929866521666140805124403","volume":"21","author":"JS Boateng","year":"2014","unstructured":"Boateng JS, Mitchell JC, Pawar H, Ayensu I (2014) Functional characterisation and permeation studies of lyophilised thiolated chitosan xerogels for buccal delivery of insulin. Protein Pept Lett 21(11):1163\u20131175","journal-title":"Protein Pept Lett"},{"key":"539_CR6","doi-asserted-by":"publisher","first-page":"104","DOI":"10.3390\/pharmaceutics11030104","volume":"11","author":"JS Boateng","year":"2019","unstructured":"Boateng JS, Okeke O (2019) Evaluation of clay-functionalized wafers and films for nicotine replacement therapy via buccal mucosa. Pharmaceutics 11:104","journal-title":"Pharmaceutics"},{"issue":"5","key":"539_CR7","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1007\/s00430-014-0343-4","volume":"203","author":"J Boros-Majewska","year":"2014","unstructured":"Boros-Majewska J, Salewska N, Borowski E, Milewski S, Malic S, Wei XQ, Hayes AJ, Wilson MJ, Williams DW (2014) Novel Nystatin A1 derivatives exhibiting low host cell toxicity and antifungal activity in an in vitro model of oral candidosis. Med Microbiol Immunol 203(5):341\u2013355","journal-title":"Med Microbiol Immunol"},{"issue":"3","key":"539_CR8","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1111\/php.12030","volume":"89","author":"J Breger","year":"2013","unstructured":"Breger J, Baeva L, Agrawal A, Shindell E, Godar DE (2013) UVB-induced inflammatory cytokine release, DNA damage and apoptosis of human oral compared with skin tissue equivalents. Photochem Photobiol 89(3):665\u2013670","journal-title":"Photochem Photobiol"},{"key":"539_CR9","doi-asserted-by":"publisher","first-page":"15779","DOI":"10.1038\/s41598-019-52115-7","volume":"9","author":"JL Brown","year":"2019","unstructured":"Brown JL, Johnston W, Delaney C, Rajendran R, Butcher J, Khan S, Bradshaw D, Ramage G, Culshaw S (2019) Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells. Sci Rep 9:15779. https:\/\/doi.org\/10.1038\/s41598-019-52115-7","journal-title":"Sci Rep"},{"issue":"4","key":"539_CR10","doi-asserted-by":"publisher","first-page":"903","DOI":"10.1177\/039463200802100414","volume":"21","author":"G Campisi","year":"2008","unstructured":"Campisi G, Paderni C, Saccone R, Siragusa MG, Lo Muzio L, Tripodo C, Giannola LI, Florena AM (2008) Carbamazepine transbuccal delivery: the histo-morphological features of reconstituted human oral epithelium and buccal porcine mucosae in the transmucosal permeation. Int J Immunopathol Pharmacol 21(4):903\u2013910","journal-title":"Int J Immunopathol Pharmacol"},{"issue":"1","key":"539_CR11","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1007\/s11626-997-0019-0","volume":"33","author":"A Delcourt-Huard","year":"1997","unstructured":"Delcourt-Huard A, Corlu A, Joffre A, Magloire H, Bonnaure-Mallet M (1997) Reconstituted human gingival epithelium: nonsubmerged in vitro model. In Vitro Cell Dev Biol Anim 33(1):30\u201336","journal-title":"In Vitro Cell Dev Biol Anim"},{"issue":"4","key":"539_CR12","doi-asserted-by":"publisher","first-page":"2012","DOI":"10.1038\/nprot.2006.323","volume":"1","author":"A Dongari-Bagtzoglou","year":"2006","unstructured":"Dongari-Bagtzoglou A, Kashleva H (2006) Development of a highly reproducible three-dimensional organotypic model of the oral mucosa. Nat Protoc 1(4):2012\u20132018","journal-title":"Nat Protoc"},{"key":"539_CR13","doi-asserted-by":"publisher","first-page":"852","DOI":"10.1158\/2159-8290.CD-18-1522","volume":"7","author":"E Driehuis","year":"2019","unstructured":"Driehuis E, Kolders S, Spelier S, L\u00f5hmussaar K, Willems SM, Devriese LA, de Bree R, de Ruiter EJ, Korving J, Begthel H, van Es JH, Geurts V, He G-W, van Jaarsveld RH, Oka R, Muraro MJ, Vivi\u00e9 J, Zandvliet MMJM, Hendrickx APA, Iakobachvili N, Sridevi P, Kranenburg O, van Boxtel R, Kops GJPL, Tuveson DA, Peters PJ, van Oudenaarden A, Clevers H (2019) Oral mucosal organoids as a potential platform for personalized cancer therapy. Cancer Discov 7:852\u2013871","journal-title":"Cancer Discov"},{"issue":"12","key":"539_CR14","doi-asserted-by":"publisher","first-page":"193","DOI":"10.2147\/CCID.S179430","volume":"22","author":"CF Gfeller","year":"2019","unstructured":"Gfeller CF, Wanser R, Mahalingam H, Moore DJ, Wang X, Lin CB, Shanga G, Grove G, Rawlings AV (2019) A series of in vitro and human studies of a novel lip cream formulation for protecting against environmental triggers of recurrent Herpes labialis. Clin Cosmet Investig Dermatol 22(12):193\u2013208","journal-title":"Clin Cosmet Investig Dermatol"},{"issue":"2","key":"539_CR15","doi-asserted-by":"publisher","first-page":"109","DOI":"10.2174\/156720110791011792","volume":"7","author":"LI Giannola","year":"2010","unstructured":"Giannola LI, De Caro V, Giandalia G, Siragusa MG, Paderni C, Campisi G, Florena AM (2010) 5-Fluorouracil buccal tablets for locoregional chemotherapy of oral squamous cell carcinoma: formulation, drug release and histological effects on reconstituted human oral epithelium and porcine buccal mucosa. Curr Drug Deliv 7(2):109\u2013117","journal-title":"Curr Drug Deliv"},{"key":"539_CR16","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.colsurfb.2013.07.019","volume":"112","author":"C Giovino","year":"2013","unstructured":"Giovino C, Ayensu I, Tetteh J, Boateng JS (2013) An integrated buccal delivery system combining chitosan films impregnated with peptide loaded PEG-b-PLA nanoparticles. Colloids Surf B: Biointerfaces 112:9\u201315","journal-title":"Colloids Surf B: Biointerfaces"},{"key":"539_CR17","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1186\/1743-422X-3-84","volume":"3","author":"R Hai","year":"2006","unstructured":"Hai R, Chu A, Li H, Umamoto S, Rider P, Liu F (2006) Infection of human cytomegalovirus in cultured human gingival tissue. Virol J 3:84","journal-title":"Virol J"},{"issue":"5","key":"539_CR18","doi-asserted-by":"publisher","first-page":"055003","DOI":"10.1088\/1468-6996\/15\/5\/055003","volume":"15","author":"S Hayakumo","year":"2014","unstructured":"Hayakumo S, Arakawa S, Takahashi M, Kondo K, Mano Y, Izumi Y (2014) Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells -in vitro studies. Sci Technol Adv Mater 15(5):055003","journal-title":"Sci Technol Adv Mater"},{"key":"539_CR19","doi-asserted-by":"publisher","first-page":"3224","DOI":"10.1038\/srep03224","volume":"3","author":"H Hisha","year":"2013","unstructured":"Hisha H, Tanaka T, Kanno S, Tokuyama Y, Komai Y, Ohe S, Yanai H, Omachi T, Ueno H (2013) Establishment of a novel lingual organoid culture system: generation of organoids having mature keratinized epithelium from adult epithelial stem cells. Sci Rep 3:3224","journal-title":"Sci Rep"},{"issue":"1\u20132","key":"539_CR20","first-page":"66","volume":"404","author":"L Hu","year":"2010","unstructured":"Hu L, Damaj BB, Martin R, Michniak-Kohn BB (2010) Enhanced in vitro transbuccal drug delivery of ondansetron HCl. Int J Pharm 404(1\u20132):66\u201374","journal-title":"Int J Pharm"},{"key":"539_CR21","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/0378-5173(95)00109-V","volume":"125","author":"J Jacobsen","year":"1995","unstructured":"Jacobsen J, van Deurs B, Pedersen M, Romer Rassing M (1995) TR146 cells grown on filters as a model for human buccal epithelium: I. morphology, growth, barrier properties, and permeability. Int J Pharm 125:165\u2013184","journal-title":"Int J Pharm"},{"key":"539_CR22","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1007\/s11046-008-9096-1","volume":"165","author":"JAMS Jayatilake","year":"2008","unstructured":"Jayatilake JAMS, Samaranayake YH, Samaranayake LPA (2008) Comparative study of candidal invasion in rabbit tongue mucosal explants and reconstituted human oral epithelium. Mycopathologia 165:373\u2013380","journal-title":"Mycopathologia"},{"key":"539_CR23","doi-asserted-by":"publisher","first-page":"33","DOI":"10.3389\/fmolb.2020.00033","volume":"7","author":"C Jensen","year":"2020","unstructured":"Jensen C, Teng Y (2020) Is it time to start transitioning from 2D to 3D cell culture? Front Mol Biosci 7:33","journal-title":"Front Mol Biosci"},{"key":"539_CR24","first-page":"101","volume":"43","author":"Y Kaluzhny","year":"2006","unstructured":"Kaluzhny Y, Kand\u00e1rov\u00e1 H, Handa Y, Deluca J, Truong T, Hunter A, Kearney P, d\u2019Argembeau L, Klausner M (2006) The EpiOcular Eye Irritation Test (EIT) for hazard identification and labelling of eye irritating chemicals: protocol optimisation for solid materials and the results after extended shipment. ATLA 43:101\u2013127","journal-title":"ATLA"},{"key":"539_CR25","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1016\/j.archoralbio.2006.05.007","volume":"51","author":"JR Kimball","year":"2006","unstructured":"Kimball JR, Nittayananta W, Klausner M, Chung WO, Dale BA (2006) Antimicrobial barrier of an in vitro oral epithelial model. Arch Oral Biol 51:775\u2013783","journal-title":"Arch Oral Biol"},{"key":"539_CR26","doi-asserted-by":"publisher","first-page":"938","DOI":"10.1016\/j.tiv.2007.01.024","volume":"21","author":"M Klausner","year":"2007","unstructured":"Klausner M, Ayehunie S, Breyfogle BA, Wertz PW, Bacca L, Kubilus J (2007) Organotypic human oral tissue models for toxicological studies. Toxicol in Vitro 21:938\u2013949","journal-title":"Toxicol in Vitro"},{"key":"539_CR27","first-page":"347","volume-title":"Advances in animal alternatives","author":"M Klausner","year":"1997","unstructured":"Klausner M, Neal PJ, Cannon CL, Kubilus J, Curren RD, Harbell JW (1997) Intra- and interlaboratory reproducibility of EpiDerm, an in vitro model for dermal irritancy testing. In: Salem H, Katz SA (eds) Advances in animal alternatives. Taylor & Francis, Washington, DC, pp 347\u2013357"},{"key":"539_CR28","doi-asserted-by":"crossref","unstructured":"Komiyama S, Miyasaka R, Kikukawa K, Hayman R (2019) Can nano-hydroxyapatite permeate the oral mucosa? A histological study using three-dimensional tissue models. PLoS One 14(4)","DOI":"10.1371\/journal.pone.0215681"},{"issue":"8","key":"539_CR29","doi-asserted-by":"publisher","first-page":"2038","DOI":"10.1128\/AAC.43.8.2038","volume":"43","author":"HC Korting","year":"1999","unstructured":"Korting HC, Schaller M, Eder G, Hamm G, B\u00f6hmer U, Hube B (1999) Effects of the human immunodeficiency virus (HIV) proteinase inhibitors saquinavir and indinavir on in vitro activities of secreted aspartyl proteinases of Candida albicans isolates from HIV-infected patients. Antimicrob Agents Chemother 43(8):2038\u20132042","journal-title":"Antimicrob Agents Chemother"},{"issue":"10","key":"539_CR30","doi-asserted-by":"publisher","first-page":"2524","DOI":"10.1016\/j.fct.2011.06.018","volume":"49","author":"F Koschier","year":"2011","unstructured":"Koschier F, Kostrubsky V, Toole C, Gallo MA (2011) In vitro effects of ethanol and mouthrinse on permeability in an oral buccal mucosal tissue construct. Food Chem Toxicol 49(10):2524\u20132929","journal-title":"Food Chem Toxicol"},{"key":"539_CR31","doi-asserted-by":"publisher","first-page":"907","DOI":"10.4161\/cbt.25935","volume":"14","author":"A Kovalchuk","year":"2013","unstructured":"Kovalchuk A, Aladedunye F, Rodriguez-Juarez R, Li D, Thomas J, Kovalchuk O, Przybylski R (2013) Novel antioxidants are not toxic to normal tissues but effectively kill cancer cells. Cancer Biol Ther 14:907\u2013915","journal-title":"Cancer Biol Ther"},{"key":"539_CR32","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1016\/j.gdata.2015.07.029","volume":"6","author":"MP Lambros","year":"2015","unstructured":"Lambros MP, DeSalvo MK, Moreno J, Mulamalla HC, Kondapalli L (2015a) Transcriptional profiling of radiation damage and preventive treatments in a 3-dimensional (3D) human cell culture model of oral mucositis. Genomics Data 6:40\u201343","journal-title":"Genomics Data"},{"key":"539_CR33","doi-asserted-by":"publisher","first-page":"Article ID 4257","DOI":"10.1155\/2015\/425760","volume":"2015","author":"MP Lambros","year":"2015","unstructured":"Lambros MP, Kondapalli L, Parsa C, Mulamalla HC, Orlando R, Pon D, Huang Y, Chow MSS (2015b) Signatures in the prevention of radiation damage by the synergistic effect of N-acetyl cysteine and Qingre Liyan Decoction, a traditional Chinese medicine, using a 3-dimensional cell culture model of oral mucositis. Evid Based Complement Altern Med 2015:Article ID 425760. https:\/\/doi.org\/10.1155\/2015\/425760","journal-title":"Evid Based Complement Altern Med"},{"key":"539_CR34","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1016\/j.bbrc.2010.12.135","volume":"405","author":"MP Lambros","year":"2011","unstructured":"Lambros MP, Parsa C, Mulamalla HC, Orlando R, Lau B, Huang Y, Pon D, Chow M (2011) Identifying cell and molecular stress after radiation in a three-dimensional (3-d) model of oral mucositis. Biochem Biophys Res Comm 405:102\u2013106","journal-title":"Biochem Biophys Res Comm"},{"key":"539_CR35","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1111\/j.1751-1097.2012.01163.x","volume":"88","author":"D Mitchell","year":"2012","unstructured":"Mitchell D, Paniker L, Godar D (2012) Nucleotide excision repair is reduced in oral epithelial tissues compared with skin. Photochem Photobiol 88:1027\u20131032","journal-title":"Photochem Photobiol"},{"key":"539_CR36","doi-asserted-by":"publisher","unstructured":"Moghaddam B, Yang J, Roohpour N (2016) Biologic evaluation of devices with chronic exposure using 3D human gingival model. Front Bioeng Biotech 4. https:\/\/doi.org\/10.3389\/conf.FBIOE.2016.01.01697","DOI":"10.3389\/conf.FBIOE.2016.01.01697"},{"key":"539_CR37","doi-asserted-by":"publisher","unstructured":"Moharamzadeh K, Brook IM, Scutt AM, Thornhill MH, Van Noort R (2008) Mucotoxicity of dental composite resins on a tissue-engineered human oral mucosal model. J Dent 36(5):331\u2013336. https:\/\/doi.org\/10.1016\/j.jdent.2008.01.019","DOI":"10.1016\/j.jdent.2008.01.019"},{"key":"539_CR38","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1099\/jmm.0.000677","volume":"67","author":"DJ Morse","year":"2018","unstructured":"Morse DJ, Wilson MJ, Wei X, Lewis MAO, Bradshaw DJ, Murdoch C, Williams DW (2018) Denture-associated biofilm infection in three-dimensional oral mucosal tissue models. J Med Microbiol 67:364\u2013375","journal-title":"J Med Microbiol"},{"key":"539_CR39","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/0022-1759(83)90303-4","volume":"65","author":"T Mosmann","year":"1983","unstructured":"Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55\u201363","journal-title":"J Immunol Methods"},{"issue":"3","key":"539_CR40","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.chom.2010.08.002","volume":"8","author":"DL Moyes","year":"2010","unstructured":"Moyes DL, Runglall M, Murciano C, Shen C, Nayar D, Thavaraj S, Kohli A, Islam A, Mora-Montes H, Challacombe SJ, Naglik JR (2010) A biphasic innate immune MAPK response discriminates between the yeast and hyphal forms of Candida albicans in epithelial cells. Cell Host Microbe 8(3):225\u2013235","journal-title":"Cell Host Microbe"},{"issue":"2\u20133","key":"539_CR41","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/S0168-3659(99)00081-4","volume":"60","author":"HM Nielsen","year":"1999","unstructured":"Nielsen HM, Verhoef JC, Ponec M, Rassing MR (1999) TR146 cells grown on filters as a model of human buccal epithelium: permeability of fluorescein isothiocyanate-labelled dextrans in the presence of sodium glycocholate. J Control Release 60(2\u20133):223\u2013233","journal-title":"J Control Release"},{"key":"539_CR42","doi-asserted-by":"crossref","unstructured":"Nishiyama K, Akagi T, Iwai S, Akashi M (2019) Construction of vascularized oral mucosa equivalents using a layer-by-layer cell coating technology. Tissue Eng C: Methods:262\u2013275","DOI":"10.1089\/ten.tec.2018.0337"},{"key":"539_CR43","doi-asserted-by":"publisher","first-page":"1013","DOI":"10.1089\/aid.2009.0032","volume":"25","author":"W Nittayananta","year":"2009","unstructured":"Nittayananta W, Hladik F, Klausner M, Harb S, Dale W (2009) HIV type 1 fails to trigger innate immune factor synthesis in differentiated oral epithelium. AIDS Res Hum Retrovir 25:1013\u20131021","journal-title":"AIDS Res Hum Retrovir"},{"key":"539_CR44","unstructured":"Office of the Surgeon General US (2004) The health consequences of smoking: a report of the surgeon general. Atlanta: Centers for Disease Control and Prevention)"},{"key":"539_CR45","doi-asserted-by":"publisher","first-page":"12","DOI":"10.1016\/j.ijpharm.2017.05.026","volume":"527","author":"L Pindakova","year":"2017","unstructured":"Pindakova L, Kasparkova V, Kejlova K, Dvorakova M, Krsek D, Jirova D, Kasparova L (2017) Behaviour of silver nanoparticles in simulated saliva and gastrointestinal fluids. Int J Pharm 527:12\u201320","journal-title":"Int J Pharm"},{"key":"539_CR46","doi-asserted-by":"publisher","first-page":"2275","DOI":"10.1093\/carcin\/bgh243","volume":"25","author":"JL Port","year":"2004","unstructured":"Port JL, Yamaguchi K, Du B, De Lorenzo M, Chang M, Heerdt PM, Kopelovich L, Marcus CB, Altorki NK, Subbaramaiah K, Dannenberg AJ (2004) Tobacco smoke induces CYP1B1 in the aerodigestive tract. Carcinogenesis 25:2275\u20132281","journal-title":"Carcinogenesis"},{"key":"539_CR47","doi-asserted-by":"publisher","first-page":"015026","DOI":"10.1088\/1748-6041\/10\/1\/015026","volume":"10","author":"SK Ramineni","year":"2015","unstructured":"Ramineni SK, Fowler CB, Fisher PD, Cunningham LL, Puleo DA (2015) Effects of epidermal growth factor loaded mucoadhesive films on wounded oral tissue rafts. Biomed Mater 10:015026. https:\/\/doi.org\/10.1088\/1748-6041\/10\/1\/015026","journal-title":"Biomed Mater"},{"key":"539_CR48","unstructured":"REGULATION (EC) No 1223\/2009 of the European Parliament and of the council of 30 November 2009 on cosmetic products. https:\/\/eur-lex.europa.eu\/legal-content\/EN\/TXT\/HTML\/?uri=CELEX:02009R1223-20150416&from=EN"},{"key":"539_CR49","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.yrtph.2005.03.005","volume":"45","author":"A Rispin","year":"2006","unstructured":"Rispin A, Stitzel K, Harbell J, Klausner M (2006) Ensuring quality of in vitro alternative test methods: current practice. Regul Toxicol Pharmacol 45:97\u2013103","journal-title":"Regul Toxicol Pharmacol"},{"issue":"4","key":"539_CR50","first-page":"621","volume":"75","author":"HT Rupniak","year":"1985","unstructured":"Rupniak HT, Rowlatt C, Lane EB, Steele JG, Trejdosiewicz LK, Laskiewicz B, Povey S, Hill BT (1985) Characteristics of four new human cell lines derived from squamous cell carcinomas of the head and neck. J Natl Cancer Inst 75(4):621\u2013635","journal-title":"J Natl Cancer Inst"},{"key":"539_CR51","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.ejps.2015.09.019","volume":"81","author":"G Sa","year":"2016","unstructured":"Sa G, Xiong X, Wu T, Yang J, He S, Zhao Y (2016) Histological features of oral epithelium in seven animal species: as a reference for selecting animal models. Eur J Pharm Sci 81:10\u201317","journal-title":"Eur J Pharm Sci"},{"key":"539_CR52","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1128\/CMR.14.2.398-429.2001","volume":"14","author":"YH Samaranayake","year":"2001","unstructured":"Samaranayake YH, Samaranayake LP (2001) Experimental oral candidiasis in animal models. Clin Microbiol Rev 14:398\u2013429","journal-title":"Clin Microbiol Rev"},{"issue":"7","key":"539_CR53","doi-asserted-by":"publisher","first-page":"470","DOI":"10.3109\/15376516.2014.943441","volume":"24","author":"WK Schlage","year":"2014","unstructured":"Schlage WK, Iskandar AR, Kostadinova R, Xiang Y, Sewer A, Majeed S, Kuehn D, Frentzel S, Talikka M, Geertz M, Mathis C, Ivanov N, Hoeng J, Peitsch MC (2014) In vitro systems toxicology approach to investigate the effects of repeated cigarette smoke exposure on human buccal and gingival organotypic epithelial tissue cultures. Toxicol Mech Methods 24(7):470\u2013487","journal-title":"Toxicol Mech Methods"},{"issue":"1\u20133","key":"539_CR54","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/S0168-3659(01)00269-3","volume":"72","author":"S Senel","year":"2001","unstructured":"Senel S, Hincal AA (2001) Drug permeation enhancement via buccal route: possibilities and limitations. J Control Release 72(1\u20133):133\u2013144","journal-title":"J Control Release"},{"issue":"2","key":"539_CR55","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1111\/j.1469-0691.2010.03248.x","volume":"17","author":"S Silva","year":"2011","unstructured":"Silva S, Hooper SJ, Henriques M, Oliveira R, Azeredo J, Williams DW (2011) The role of secreted aspartyl proteinases in Candida tropicalis invasion and damage of oral mucosa. Clin Microbiol Infect 17(2):264\u2013272","journal-title":"Clin Microbiol Infect"},{"issue":"3","key":"539_CR56","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1517\/17425247.2.3.507","volume":"2","author":"JD Smart","year":"2005","unstructured":"Smart JD (2005) Buccal drug delivery. Expert Opin Drug Deliv 2(3):507\u2013517","journal-title":"Expert Opin Drug Deliv"},{"key":"539_CR57","doi-asserted-by":"publisher","first-page":"1617","DOI":"10.1208\/s12249-016-0630-z","volume":"18","author":"G Song","year":"2017","unstructured":"Song G, Banov D, Bassani AS, Valdez BC (2017) Evaluation of the safety, cell migration, and mucoadhesive properties of a mucoadhesive polymer blend in human oral mucosa. AAPS PharmSciTech 18:1617\u20131623","journal-title":"AAPS PharmSciTech"},{"key":"539_CR58","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1093\/oxfordjournals.jncimonographs.a003443","volume":"29","author":"CA Squier","year":"2001","unstructured":"Squier CA, Kremer MJ (2001) Biology of oral mucosa and esophagus. J Natl Cancer Inst Monogr 29:7\u201315","journal-title":"J Natl Cancer Inst Monogr"},{"issue":"47","key":"539_CR59","doi-asserted-by":"publisher","first-page":"77196","DOI":"10.18632\/oncotarget.12857","volume":"7","author":"IK Sundar","year":"2016","unstructured":"Sundar IK, Javed F, Romanos GE, Rahman I (2016) E-cigarettes and flavorings induce inflammatory and prosenescence responses in oral epithelial cells and periodontal fibroblasts. Oncotarget 7(47):77196\u201377204","journal-title":"Oncotarget"},{"key":"539_CR60","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1080\/03639040600901994","volume":"33","author":"RA Thakur","year":"2007","unstructured":"Thakur RA, Michniak BB, Meidan VM (2007) Transdermal and buccal delivery of methylxanthines through human tissue in vitro. Drug Dev Ind Pharm 33:513\u2013521","journal-title":"Drug Dev Ind Pharm"},{"issue":"1","key":"539_CR61","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1093\/ejo\/cjl063","volume":"29","author":"B Vande Vannet","year":"2007","unstructured":"Vande Vannet B, Hanssens JL, Wehrbein H (2007) The use of three-dimensional oral mucosa cell cultures to assess the toxicity of soldered and welded wires. Eur J Orthod 29(1):60\u201366","journal-title":"Eur J Orthod"},{"issue":"5","key":"539_CR62","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1093\/ejo\/cjl002","volume":"28","author":"B Vande Vannet","year":"2006","unstructured":"Vande Vannet B, Mohebbian N, Wehrbein H (2006) Toxicity of used orthodontic archwires assessed by three-dimensional cell culture. Eur J Orthod 28(5):426\u2013432","journal-title":"Eur J Orthod"},{"issue":"4","key":"539_CR63","doi-asserted-by":"publisher","first-page":"716","DOI":"10.2319\/052706-212.1","volume":"77","author":"BMRA Vande Vannet","year":"2007","unstructured":"Vande Vannet BMRA, Hanssens JL (2007) Cytotoxicity of two bonding adhesives assessed by three-dimensional cell culture. Angle Orthod 77(4):716\u2013722","journal-title":"Angle Orthod"},{"key":"539_CR64","doi-asserted-by":"publisher","first-page":"2020040315","DOI":"10.20944\/preprints202004.0315.v1","volume":"2020","author":"B Wang","year":"2020","unstructured":"Wang B, Kovalchuk A, Li D, Ilnytskyy Y, Kovalchuk I, Kovalchuk O (2020) In search of preventative strategies: novel anti-inflammatory high-CBD cannabis sativa extracts modulate ACE2 expression in COVID-19 gateway tissues. Preprints 2020:2020040315. https:\/\/doi.org\/10.20944\/preprints202004.0315.v1","journal-title":"Preprints"},{"key":"539_CR65","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1016\/j.micpath.2010.11.009","volume":"50","author":"NP Yadev","year":"2011","unstructured":"Yadev NP, Murdoch C, Saville SP, Thornhill MH (2011) Evaluation of tissue engineered models of the oral mucosa to investigate oral candidiasis. Microb Pathog 50:278\u2013285","journal-title":"Microb Pathog"},{"issue":"Suppl 3","key":"539_CR66","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1111\/j.1875-595X.2011.00048.x","volume":"61","author":"J Yang","year":"2011","unstructured":"Yang J, Deol G, Myangar N (2011) Retention of o-cymen-5-ol and zinc on reconstructed gingival tissue from a toothpaste formulation. Int Dentral J 61(Suppl 3):41\u201345","journal-title":"Int Dentral J"},{"issue":"6","key":"539_CR67","doi-asserted-by":"publisher","first-page":"e1006457","DOI":"10.1371\/journal.ppat.1006457","volume":"13","author":"S Yost","year":"2017","unstructured":"Yost S, Duran-Pinedo AE, Krishnan K, Frias-Lopez J (2017) Potassium is a key signal in host-microbiome dysbiosis in periodontitis. PLoS Pathog 13(6):e1006457","journal-title":"PLoS Pathog"},{"key":"539_CR68","doi-asserted-by":"publisher","first-page":"1252","DOI":"10.1021\/acs.chemrestox.6b00174","volume":"29","author":"F Zanetti","year":"2016","unstructured":"Zanetti F, Sewer A, Mathis C, Iskandar AR, Kostadinova R, Schlage WK, Leroy P, Majeed S, Guedj E, Trivedi K, Martin F, Elamin A, Merg C, Ivanov NV, Frentzel S, Peitsch MC, Hoeng J (2016) Systems toxicology assessment of the biological impact of a candidate modified risk tobacco product on human organotypic oral epithelial cultures. Chem Res Toxicol 29:1252\u20131269","journal-title":"Chem Res Toxicol"}],"container-title":["In Vitro Cellular & Developmental Biology - Animal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11626-020-00539-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11626-020-00539-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11626-020-00539-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,9]],"date-time":"2025-04-09T02:44:17Z","timestamp":1744166657000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11626-020-00539-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,14]]},"references-count":68,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,2]]}},"alternative-id":["539"],"URL":"https:\/\/doi.org\/10.1007\/s11626-020-00539-1","relation":{},"ISSN":["1071-2690","1543-706X"],"issn-type":[{"value":"1071-2690","type":"print"},{"value":"1543-706X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,14]]},"assertion":[{"value":"25 June 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 December 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 January 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}}]}}