{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T15:06:43Z","timestamp":1782832003547,"version":"3.54.5"},"reference-count":32,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"rusa","award":["RUSA\/Order\/2016-17\/270 Dated 18\/06\/2016"],"award-info":[{"award-number":["RUSA\/Order\/2016-17\/270 Dated 18\/06\/2016"]}]},{"name":"rusa","award":["RUSA\/Order\/2016-17\/284 Dated 19\/06\/2016"],"award-info":[{"award-number":["RUSA\/Order\/2016-17\/284 Dated 19\/06\/2016"]}]},{"name":"sarathi","award":["CIN-U74999PN2018NPL177394"],"award-info":[{"award-number":["CIN-U74999PN2018NPL177394"]}]},{"name":"purse","award":["SR\/PURSE PHASE 2\/24 (C) Dated 8 March 2018"],"award-info":[{"award-number":["SR\/PURSE PHASE 2\/24 (C) Dated 8 March 2018"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["In Vitro Cell.Dev.Biol.-Animal"],"published-print":{"date-parts":[[2022,5]]},"DOI":"10.1007\/s11626-022-00690-x","type":"journal-article","created":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T16:03:07Z","timestamp":1654531387000},"page":"376-383","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["In vitro differentiation of human pancreatic duct\u2013derived PANC-1 cells into \u03b2-cell phenotype using Tinospora cordifolia"],"prefix":"10.1007","volume":"58","author":[{"given":"Hemangee","family":"Damame","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheetalnath","family":"Rooge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul","family":"Patil","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chandramukhi","family":"Garad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akalpita","family":"Arvindekar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,6]]},"reference":[{"key":"690_CR1","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.mce.2016.11.008","volume":"445","author":"S Afelik","year":"2017","unstructured":"Afelik S, Rovira M (2017) Pancreatic \u03b2-cell regeneration: facultative or dedicated progenitors? Mol Cell Endocrinol 445:85\u201394","journal-title":"Mol Cell Endocrinol"},{"key":"690_CR2","doi-asserted-by":"crossref","unstructured":"Ansarullah, Bharucha B, Umarani M, Dwivedi M, Ladha N, Rasheedunnisa B, Hardikar A, Ramchandran A (2012) Oreocnide integrifolia flavonoids augment reprogramming for islet neogenesis and \u03b2-cell regeneration in pancreatectomized BALB\/c mice. Evid Based Complement Alternat Med 2012:260467","DOI":"10.1155\/2012\/260467"},{"key":"690_CR3","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1210\/endo-112-3-1049","volume":"112","author":"V Bonnevie-Nielsen","year":"1983","unstructured":"Bonnevie-Nielsen V, Skovgaard L, Lernmark A (1983) \u03b2-cell functions relative to islet volume and hormone content in the isolated perfused mouse pancreas. Endocrinology 112:1049\u20131056","journal-title":"Endocrinology"},{"key":"690_CR4","doi-asserted-by":"publisher","first-page":"998","DOI":"10.1080\/14756360802565346","volume":"24","author":"A Chougale","year":"2009","unstructured":"Chougale A, Ghadyale V, Panaskar S, Arvindekar A (2009) Alpha glucosidase inhibition by stem extract of Tinospora cordifolia. J Enzyme Inhib Med Chem 24:998\u20131001","journal-title":"J Enzyme Inhib Med Chem"},{"key":"690_CR5","first-page":"456","volume":"16","author":"E Corritore","year":"2014","unstructured":"Corritore E, Dugnani E, Pasquale V, Misawa R, Witkowski P, Lei J, Markmann J, Piemonti L, Sokal E, Bonner-Weir S, Lysy P (2014) \u03b2-cell differentiation of human pancreatic duct derived cells after in vitro expansion. Cell Rep 16:456\u2013466","journal-title":"Cell Rep"},{"key":"690_CR6","doi-asserted-by":"publisher","first-page":"280392","DOI":"10.1155\/2013\/280392","volume":"2013","author":"N Dadheech","year":"2013","unstructured":"Dadheech N, Soni S, Srivastava A, Dadheech S, Gupta S, Gopurappilly R, Bhonde RR, Gupta S (2013) A small molecule Swertisin from Enicostemma Littorale differentiates NIH3T3 cells into islet like clusters and restores normoglycemia upon transplantation in diabetic Balb\/c Mice. Evid Based Complement Alternat Med 2013:280392","journal-title":"Evid Based Complement Alternat Med"},{"key":"690_CR7","doi-asserted-by":"publisher","first-page":"100072","DOI":"10.1016\/j.phyplu.2021.100072","volume":"1","author":"H Damame","year":"2021","unstructured":"Damame H, Rooge S, Patil R, Garad C, Arvindekar A (2021) Beta cell protective effect of Curcuma longa and Piper nigrum in cytokine cocktail induced apoptosis in Min6 pancreatic beta cells. Phytomedicine Plus 1:100072","journal-title":"Phytomedicine Plus"},{"key":"690_CR8","doi-asserted-by":"publisher","first-page":"2234","DOI":"10.3390\/ijms18112234","volume":"18","author":"G Donadel","year":"2017","unstructured":"Donadel G, Pastore D, Della-Morte D, Capuani B, Lombardo MF, Pacifici F, Bugliani M, Grieco FA, Marchetti P, Lauro D (2017) FGF-2b and h-PL transform duct and non-endocrine human pancreatic cells into endocrine insulin secreting cells by modulating differentiating genes. Int J Mol Sci 18:2234","journal-title":"Int J Mol Sci"},{"key":"690_CR9","first-page":"49","volume":"9","author":"S Gupta","year":"2010","unstructured":"Gupta S, Dadheech N, Singh A, Soni S, Bhonde R (2010) Enicostemma Littorale: a new therapeutic target for islet neogenesis. Int J Integr Biol 9:49","journal-title":"Int J Integr Biol"},{"key":"690_CR10","doi-asserted-by":"publisher","first-page":"1034","DOI":"10.1210\/jc.2009-1819","volume":"95","author":"P Halban","year":"2010","unstructured":"Halban P, German M, Kahn S, Weir G (2010) Current status of islet cell replacement and regeneration therapy. J Clin Endocrinol Metab 95:1034\u20131043","journal-title":"J Clin Endocrinol Metab"},{"key":"690_CR11","first-page":"53","volume":"10","author":"W Hansen","year":"1989","unstructured":"Hansen W, Christie M, Kahn R, Norgaard A, Abel I, Petersen A, Jorgensen D, Baekkeskov S, Nielsen J, Lernmark A et al (1989) Supravital dithizone staining in the isolation of human and rat pancreatic islets. Diabetes Res 10:53\u201357","journal-title":"Diabetes Res"},{"key":"690_CR12","doi-asserted-by":"publisher","first-page":"e0224344","DOI":"10.1371\/journal.pone.0224344","volume":"15","author":"H Hohmeier","year":"2020","unstructured":"Hohmeier H, Zhang L, Taylor B, Stephens S, Lu D, McNamara P, Laffitte B, Newgard C (2020) Identification of a small molecule that stimulates human \u03b2-cell proliferation and insulin secretion, and protects against cytotoxic stress in rat insulinoma cells. PLoS One 15:e0224344","journal-title":"PLoS One"},{"key":"690_CR13","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1530\/eje.0.1460129","volume":"146","author":"H Hui","year":"2002","unstructured":"Hui H, Perfetti R (2002) Pancreas duodenum homeobox-1 regulates pancreas development during embryogenesis and islet cell function in adulthood. Eur J Endocrinol 146:129\u2013141","journal-title":"Eur J Endocrinol"},{"key":"690_CR14","doi-asserted-by":"publisher","first-page":"1668","DOI":"10.7150\/ijms.36011","volume":"16","author":"Y Hwang","year":"2019","unstructured":"Hwang Y, Cha SH, Hong Y, Jung A, Jun HS (2019) Direct differentiation of insulin-producing cells from human urine-derived stem cells. Int J Med Sci 16:1668\u20131676","journal-title":"Int J Med Sci"},{"key":"690_CR15","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1186\/s13036-017-0066-3","volume":"11","author":"E Jacobson","year":"2017","unstructured":"Jacobson E, Tzanakakis E (2017) Human pluripotent stem cell differentiation to functional pancreatic cells for diabetes therapies: Innovations, challenges and future directions. J Biol Eng 11:21","journal-title":"J Biol Eng"},{"key":"690_CR16","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1038\/nature00870","volume":"418","author":"Y Jiang","year":"2002","unstructured":"Jiang Y, Jahagirdar B, Reinhardt R, Schwartz R, Keene C, Ortiz-Gonzalez X, Reyes M, Lenvik T, Lund T, Blackstad M, Du J, Aldrich S, Lisberg A, Low W, Largaespada D, Verfaillie C (2002) Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418:41\u201349","journal-title":"Nature"},{"key":"690_CR17","doi-asserted-by":"publisher","first-page":"837","DOI":"10.1046\/j.1365-2036.2003.01738.x","volume":"18","author":"M Juh\u00e1sz","year":"2003","unstructured":"Juh\u00e1sz M, Chen J, Lendeckel U, Kellner U, Kasper H, Tulassay Z, Pastorekova S, Malfertheiner P, Ebert M (2003) Expression of carbonic anhydrase IX in human pancreatic cancer. Aliment Pharmacol Ther 18:837\u2013846","journal-title":"Aliment Pharmacol Ther"},{"key":"690_CR18","doi-asserted-by":"publisher","first-page":"923","DOI":"10.1016\/j.biocel.2005.09.019","volume":"38","author":"I Kojima","year":"2006","unstructured":"Kojima I, Umezawa K (2006) Conophylline: a novel differentiation inducer for pancreatic beta cells. Int J Biochem Cell Biol 38:923\u2013930","journal-title":"Int J Biochem Cell Biol"},{"key":"690_CR19","doi-asserted-by":"publisher","first-page":"827","DOI":"10.1097\/00007890-198804000-00038","volume":"45","author":"ZA Latif","year":"1988","unstructured":"Latif ZA, Noel J, Alejandro R (1988) A simple method of staining fresh and cultured islets. Transplantation 45:827\u2013830","journal-title":"Transplantation"},{"key":"690_CR20","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1186\/s13287-020-02080-0","volume":"12","author":"Y Lee","year":"2021","unstructured":"Lee Y, Yi H, Seo E, Oh J, Lee S, Ferber S, Okano T, Shim I, Kim S (2021) Improvement of the therapeutic capacity of insulin-producing cells trans-differentiated from human liver cells using engineered cell sheet. Stem Cell Res Ther 12:3","journal-title":"Stem Cell Res Ther"},{"key":"690_CR21","doi-asserted-by":"publisher","first-page":"741","DOI":"10.1002\/ijc.2910150505","volume":"15","author":"M Lieber","year":"1975","unstructured":"Lieber M, Mazzetta J, Nelson-Rees W, Kaplan M, Todaro G (1975) Establishment of continuous cell line (PANC-1) from human carcinoma of the exocrine pancreas. Int J Cancer 15:741\u2013749","journal-title":"Int J Cancer"},{"key":"690_CR22","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1007\/s00125-001-0728-y","volume":"45","author":"D Melloul","year":"2002","unstructured":"Melloul D, Marshak S, Cerasi E (2002) Regulation of insulin gene transcription. Diabetologia 45:309\u2013326","journal-title":"Diabetologia"},{"key":"690_CR23","doi-asserted-by":"publisher","first-page":"1364","DOI":"10.1073\/pnas.0336479100","volume":"100","author":"E Mezey","year":"2003","unstructured":"Mezey E, Key S, Vogelsang G, Szalayova I, Lange G, Crain B (2003) Transplanted bone marrow generates new neurons in human brains. Proc Natl Acad Sci U S A 100:1364\u20131369","journal-title":"Proc Natl Acad Sci U S A"},{"key":"690_CR24","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1007\/s13105-012-0228-1","volume":"69","author":"P Moshtagh","year":"2013","unstructured":"Moshtagh P, Emami S, Sharifi A (2013) Differentiation of human adipose-derived mesenchymal stem cell into insulin-producing cells: an in vitro study. J Physiol Biochem 69:451\u2013458","journal-title":"J Physiol Biochem"},{"key":"690_CR25","doi-asserted-by":"publisher","first-page":"2472","DOI":"10.1242\/dev.093187","volume":"140","author":"F Pagliuca","year":"2013","unstructured":"Pagliuca F, Melton D (2013) How to make a functional \u03b2-cell. Development 140:2472\u20132483","journal-title":"Development"},{"key":"690_CR26","doi-asserted-by":"publisher","first-page":"2408","DOI":"10.1016\/j.celrep.2018.02.006","volume":"22","author":"M Qadir","year":"2018","unstructured":"Qadir M, \u00c1lvarez-Cubela S, Klein D, Lanzoni G, Garc\u00eda-Santana C, Montalvo A, Pl\u00e1ceres-Uray F, Mazza E, Ricordi C, Inverardi L, Pastori R, Dom\u00ednguez-Bendala J (2018) P2RY1\/ALK3-expressing cells within the adult human exocrine pancreas are BMP-7 expandable and exhibit progenitor-like characteristics. Cell Rep 22:2408\u20132420","journal-title":"Cell Rep"},{"key":"690_CR27","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1111\/j.1463-1326.2008.00969.x","volume":"4","author":"J Rahier","year":"2008","unstructured":"Rahier J, Guiot Y, Goebbels R, Sempoux C, Henquin J (2008) Pancreatic beta-cell mass in European subjects with type 2 diabetes. Diabetes Obes Metab 4:32\u201342","journal-title":"Diabetes Obes Metab"},{"key":"690_CR28","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.cbi.2015.11.021","volume":"243","author":"M Rajalakshmi","year":"2016","unstructured":"Rajalakshmi M, Anita R (2016) \u03b2-cell regenerative efficacy of a polysaccharide isolated from methanolic extract of Tinospora cordifolia stem on streptozotocin-induced diabetic Wistar rats. Chem Biol Interact 243:45\u201353","journal-title":"Chem Biol Interact"},{"key":"690_CR29","doi-asserted-by":"publisher","first-page":"267","DOI":"10.3109\/10520295209105089","volume":"27","author":"H Scott","year":"1952","unstructured":"Scott H (1952) Rapid staining of beta cell granules in pancreatic islets. Stain Technol 27:267\u2013268","journal-title":"Stain Technol"},{"key":"690_CR30","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/S2221-1691(15)30173-8","volume":"5","author":"R Sharma","year":"2015","unstructured":"Sharma R, Amin H, Galib PP (2015) Antidiabetic claims of Tinospora cordifolia (wild.) milers: critical appraisal and role of therapy. Asian Pac J Trop Biomed 5:68\u201378","journal-title":"Asian Pac J Trop Biomed"},{"key":"690_CR31","doi-asserted-by":"publisher","first-page":"383","DOI":"10.1016\/j.bbrc.2009.03.114","volume":"383","author":"B Xia","year":"2009","unstructured":"Xia B, Zhan X, Yi R, Yang B (2009) Can pancreatic duct-derived progenitors be a source of islet regeneration? Biochem Biophys Res Commun 383:383\u2013385","journal-title":"Biochem Biophys Res Commun"},{"key":"690_CR32","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1186\/s13287-020-01694-8","volume":"11","author":"XF Yang","year":"2020","unstructured":"Yang XF, Zhou SY, Wang C, Huang W, Li N, He F, Li FR (2020) Inhibition of LSD1 promotes the differentiation of human induced pluripotent stem cells into insulin-producing cells. Stem Cell Res Ther 11:185","journal-title":"Stem Cell Res Ther"}],"container-title":["In Vitro Cellular &amp; Developmental Biology - Animal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11626-022-00690-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11626-022-00690-x\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11626-022-00690-x.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T13:23:53Z","timestamp":1655817833000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11626-022-00690-x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5]]},"references-count":32,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["690"],"URL":"https:\/\/doi.org\/10.1007\/s11626-022-00690-x","relation":{},"ISSN":["1071-2690","1543-706X"],"issn-type":[{"value":"1071-2690","type":"print"},{"value":"1543-706X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5]]},"assertion":[{"value":"2 February 2022","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 May 2022","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 June 2022","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}