{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T15:40:09Z","timestamp":1748619609238,"version":"3.41.0"},"reference-count":42,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100006567","name":"Suhara Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006567","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007591","name":"Akiyama Life Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007591","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K05950"],"award-info":[{"award-number":["21K05950"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["In Vitro Cell.Dev.Biol.-Animal"],"published-print":{"date-parts":[[2025,4]]},"DOI":"10.1007\/s11626-025-01043-0","type":"journal-article","created":{"date-parts":[[2025,5,15]],"date-time":"2025-05-15T18:26:46Z","timestamp":1747333606000},"page":"416-424","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Epidermal growth factor increases cystathionine \u03b2-synthase expression in cultured embryonic spinal cord cells"],"prefix":"10.1007","volume":"61","author":[{"given":"Ryota","family":"Eguchi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuya","family":"Higashida","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mizuki","family":"Oouchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soichiro","family":"Yamaguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2481-5592","authenticated-orcid":false,"given":"Ken-ichi","family":"Otsuguro","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,5,15]]},"reference":[{"key":"1043_CR1","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1002\/jnr.10279","volume":"69","author":"T Adachi","year":"2002","unstructured":"Adachi T, Takanaga H, Sakurai Y, Ishido M, Kunimoto M, Asou H (2002) Influence of cell density and thyroid hormone on glial cell development in primary cultures of embryonic rat cerebral hemisphere. J Neurosci Res 69:61\u201371","journal-title":"J Neurosci Res"},{"key":"1043_CR2","doi-asserted-by":"publisher","first-page":"1994","DOI":"10.1093\/hmg\/ddn097","volume":"17","author":"N Akahoshi","year":"2008","unstructured":"Akahoshi N, Kobayashi C, Ishizaki Y, Izumi T, Himi T, Suematsu M, Ishii I (2008) Genetic background conversion ameliorates semi-lethality and permits behavioral analyses in cystathionine beta-synthase-deficient mice, an animal model for hyperhomocysteinemia. Hum Mol Genet 17:1994\u20132005","journal-title":"Hum Mol Genet"},{"key":"1043_CR3","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/S0896-6273(00)80937-X","volume":"19","author":"RC Burrows","year":"1997","unstructured":"Burrows RC, Wancio D, Levitt P, Lillien L (1997) Response diversity and the timing of progenitor cell maturation are regulated by developmental changes in EGFR expression in the cortex. Neuron 19:251\u2013267","journal-title":"Neuron"},{"key":"1043_CR4","doi-asserted-by":"publisher","first-page":"4489","DOI":"10.1096\/fj.13-234716","volume":"27","author":"M Campolo","year":"2013","unstructured":"Campolo M, Esposito E, Ahmad A, Di Paola R, Wallace JL, Cuzzocrea S (2013) A hydrogen sulfide-releasing cyclooxygenase inhibitor markedly accelerates recovery from experimental spinal cord injury. FASEB J 27:4489\u20134499","journal-title":"FASEB J"},{"key":"1043_CR5","doi-asserted-by":"publisher","first-page":"1854","DOI":"10.1096\/fj.05-3724fje","volume":"19","author":"Y Enokido","year":"2005","unstructured":"Enokido Y, Suzuki E, Iwasawa K, Namekata K, Okazawa H, Kimura H (2005) Cystathionine \u03b2-synthase, a key enzyme for homocysteine metabolism, is preferentially expressed in the radial glia\/astrocyte lineage of developing mouse CNS. FASEB J 19:1854\u20131856","journal-title":"FASEB J"},{"key":"1043_CR6","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1007\/BF02623400","volume":"15","author":"S Fedoroff","year":"1979","unstructured":"Fedoroff S, Hall C (1979) Effect of horse serum on neural cell differentiation in tissue culture. In Vitro 15:641\u2013648","journal-title":"In Vitro"},{"key":"1043_CR7","doi-asserted-by":"publisher","first-page":"1353","DOI":"10.1083\/jcb.104.5.1353","volume":"104","author":"ME Hatten","year":"1987","unstructured":"Hatten ME (1987) Neuronal inhibition of astroglial cell proliferation is membrane mediated. J Cell Biol 104:1353\u20131360","journal-title":"J Cell Biol"},{"key":"1043_CR8","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1089\/ars.2010.3671","volume":"15","author":"K Kida","year":"2011","unstructured":"Kida K, Yamada M, Tokuda K, Marutani E, Kakinohana M, Kaneki M, Ichinose F (2011) Inhaled hydrogen sulfide prevents neurodegeneration and movement disorder in a mouse model of Parkinson\u2019s disease. Antioxid Redox Signal 15:343\u2013352","journal-title":"Antioxid Redox Signal"},{"key":"1043_CR9","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.niox.2014.01.002","volume":"41","author":"H Kimura","year":"2014","unstructured":"Kimura H (2014) The physiological role of hydrogen sulfide and beyond. Nitric Oxide 41:4\u201310","journal-title":"Nitric Oxide"},{"key":"1043_CR10","doi-asserted-by":"publisher","first-page":"1165","DOI":"10.1096\/fj.04-1815fje","volume":"18","author":"Y Kimura","year":"2004","unstructured":"Kimura Y, Kimura H (2004) Hydrogen sulfide protects neurons from oxidative stress. FASEB J 18:1165\u20131167","journal-title":"FASEB J"},{"key":"1043_CR11","doi-asserted-by":"publisher","first-page":"2451","DOI":"10.1096\/fj.12-226415","volume":"27","author":"Y Kimura","year":"2013","unstructured":"Kimura Y, Mikami Y, Osumi K, Tsugane M, Oka J, Kimura H (2013) Polysulfides are possible H2S-derived signaling molecules in rat brain. FASEB J 27:2451\u20132457","journal-title":"FASEB J"},{"key":"1043_CR12","first-page":"1589747","volume":"2016","author":"B Kocer","year":"2016","unstructured":"Kocer B, Guven H, Conkbayir I, Comoglu SS, Delibas S (2016) The effect of hyperhomocysteinemia on motor symptoms, cognitive status, and vascular risk in patients with Parkinson\u2019s disease. Parkinsons Dis 2016:1589747","journal-title":"Parkinsons Dis"},{"key":"1043_CR13","doi-asserted-by":"publisher","first-page":"1299","DOI":"10.1002\/glia.20698","volume":"56","author":"A Koulakoff","year":"2008","unstructured":"Koulakoff A, Ezan P, Giaume C (2008) Neurons control the expression of connexin 30 and connexin 43 in mouse cortical astrocytes. Glia 56:1299\u20131311","journal-title":"Glia"},{"key":"1043_CR14","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1038\/sj.sc.3101873","volume":"44","author":"MY Lee","year":"2006","unstructured":"Lee MY, Myers J, Abella J, Froelicher VF, Perkash I, Kiratli BJ (2006) Homocysteine and hypertension in persons with spinal cord injury. Spinal Cord 44:474\u2013479","journal-title":"Spinal Cord"},{"key":"1043_CR15","doi-asserted-by":"publisher","first-page":"1571","DOI":"10.1002\/glia.23630","volume":"67","author":"J Li","year":"2019","unstructured":"Li J, Khankan RR, Caneda C, Godoy MI, Haney MS, Krawczyk MC, Bassik MC, Sloan SA, Zhang Y (2019) Astrocyte-to-astrocyte contact and a positive feedback loop of growth factor signaling regulate astrocyte maturation. Glia 67:1571\u20131597","journal-title":"Glia"},{"key":"1043_CR16","doi-asserted-by":"publisher","first-page":"4993","DOI":"10.1242\/dev.127.22.4993","volume":"127","author":"L Lillien","year":"2000","unstructured":"Lillien L, Raphael H (2000) BMP and FGF regulate the development of EGF-responsive neural progenitor cells. Development 127:4993\u20135005","journal-title":"Development"},{"key":"1043_CR17","doi-asserted-by":"publisher","first-page":"104787","DOI":"10.1016\/j.neuint.2020.104787","volume":"139","author":"Z Martinez-Lozada","year":"2020","unstructured":"Martinez-Lozada Z, Robinson MB (2020) Reciprocal communication between astrocytes and endothelial cells is required for astrocytic glutamate transporter 1 (GLT-1) expression. Neurochem Int 139:104787","journal-title":"Neurochem Int"},{"key":"1043_CR18","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/j.neures.2015.03.003","volume":"97","author":"R Miyamoto","year":"2015","unstructured":"Miyamoto R, Otsuguro K, Yamaguchi S, Ito S (2015) Neuronal regulation of expression of hydrogen sulfide-producing enzyme cystathionine \u03b2-synthase in rat spinal cord astrocytes. Neurosci Res 97:52\u201359","journal-title":"Neurosci Res"},{"key":"1043_CR19","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1096\/fj.03-1052fje","volume":"18","author":"Y Nagai","year":"2004","unstructured":"Nagai Y, Tsugane M, Oka J, Kimura H (2004) Hydrogen sulfide induces calcium waves in astrocytes. FASEB J 18:557\u2013559","journal-title":"FASEB J"},{"key":"1043_CR20","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.neures.2021.01.008","volume":"171","author":"T Nii","year":"2021","unstructured":"Nii T, Eguchi R, Otsuguro K (2021b) Hydrogen sulfide induces Ca2+ release from intracellular Ca2+ stores and stimulates lactate production in spinal cord astrocytes. Neurosci Res 171:67\u201373","journal-title":"Neurosci Res"},{"key":"1043_CR21","doi-asserted-by":"publisher","first-page":"173684","DOI":"10.1016\/j.ejphar.2020.173684","volume":"891","author":"T Nii","year":"2021","unstructured":"Nii T, Eguchi R, Yamaguchi S, Otsuguro K (2021a) Hydrogen sulfide induces Ca2+ release from the endoplasmic reticulum and suppresses ATP-induced Ca2+ signaling in rat spinal cord astrocytes. Eur J Pharmacol 891:173684","journal-title":"Eur J Pharmacol"},{"key":"1043_CR22","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1007\/BF01271539","volume":"100","author":"B Regland","year":"1995","unstructured":"Regland B, Johansson BV, Grenfeldt B, Hjelmgren LT, Medhus M (1995) Homocysteinemia is a common feature of schizophrenia. J Neural Transm Gen Sect 100:165\u2013169","journal-title":"J Neural Transm Gen Sect"},{"key":"1043_CR23","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1177\/002215540305100311","volume":"51","author":"K Robert","year":"2003","unstructured":"Robert K, Vialard F, Thiery E, Toyama K, Sinet PM, Janel N, London J (2003) Expression of the cystathionine \u03b2 synthase (CBS) gene during mouse development and immunolocalization in adult brain. J Histochem Cytochem 51:363\u2013371","journal-title":"J Histochem Cytochem"},{"key":"1043_CR24","doi-asserted-by":"publisher","first-page":"1513","DOI":"10.1083\/jcb.149.7.1513","volume":"149","author":"N Rouach","year":"2000","unstructured":"Rouach N, Glowinski J, Giaume C (2000) Activity-dependent neuronal control of gap-junctional communication in astrocytes. J Cell Biol 149:1513\u20131526","journal-title":"J Cell Biol"},{"key":"1043_CR25","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1038\/nature09611","volume":"468","author":"DH Rowitch","year":"2010","unstructured":"Rowitch DH, Kriegstein AR (2010) Developmental genetics of vertebrate glial-cell specification. Nature 468:214\u2013222","journal-title":"Nature"},{"key":"1043_CR26","doi-asserted-by":"crossref","unstructured":"Sato M, Nakahara K, Goto S, Kaiya H, Miyazato M, Date Y, Nakazato M, Kangawa K, Murakami N (2006) Effects of ghrelin and des-acyl ghrelin on neurogenesis of the rat fetal spinal cord. Biochem Biophys Res Commun 350:598\u2013603","DOI":"10.1016\/j.bbrc.2006.09.088"},{"key":"1043_CR27","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1124\/mol.53.3.355","volume":"53","author":"BD Schlag","year":"1998","unstructured":"Schlag BD, Vondrasek JR, Munir M, Kalandadze A, Zelenaia OA, Rothstein JD, Robinson MB (1998) Regulation of the glial Na+-dependent glutamate transporters by cyclic AMP analogs and neurons. Mol Pharmacol 53:355\u2013369","journal-title":"Mol Pharmacol"},{"key":"1043_CR28","doi-asserted-by":"publisher","first-page":"476","DOI":"10.1056\/NEJMoa011613","volume":"346","author":"S Seshadri","year":"2002","unstructured":"Seshadri S, Beiser A, Selhub J, Jacques PF, Rosenberg IH, D\u2019Agostino RB, Wilson PW, Wolf PA (2002) Plasma homocysteine as a risk factor for dementia and Alzheimer\u2019s disease. N Engl J Med 346:476\u2013483","journal-title":"N Engl J Med"},{"key":"1043_CR29","doi-asserted-by":"publisher","first-page":"588","DOI":"10.1016\/S0021-9258(17)43702-1","volume":"259","author":"F Skovby","year":"1984","unstructured":"Skovby F, Kraus JP, Rosenberg LE (1984) Biosynthesis and proteolytic activation of cystathionine beta-synthase in rat liver. J Biol Chem 259:588\u2013593","journal-title":"J Biol Chem"},{"key":"1043_CR30","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1038\/nature24638","volume":"551","author":"JA Stogsdill","year":"2017","unstructured":"Stogsdill JA, Ramirez J, Liu D, Kim YH, Baldwin KT, Enustun E, Ejikeme T, Ji RR, Eroglu C (2017) Astrocytic neuroligins control astrocyte morphogenesis and synaptogenesis. Nature 551:192\u2013197","journal-title":"Nature"},{"key":"1043_CR31","doi-asserted-by":"publisher","first-page":"932","DOI":"10.1523\/JNEUROSCI.17-03-00932.1997","volume":"17","author":"RA Swanson","year":"1997","unstructured":"Swanson RA, Liu J, Miller JW, Rothstein JD, Farrell K, Stein BA, Longuemare MC (1997) Neuronal regulation of glutamate transporter subtype expression in astrocytes. J Neurosci 17:932\u2013940","journal-title":"J Neurosci"},{"key":"1043_CR32","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1038\/nrd2425","volume":"6","author":"C Szab\u00f3","year":"2007","unstructured":"Szab\u00f3 C (2007) Hydrogen sulphide and its therapeutic potential. Nat Rev Drug Discov 6:917\u2013935","journal-title":"Nat Rev Drug Discov"},{"key":"1043_CR33","doi-asserted-by":"publisher","first-page":"1386","DOI":"10.7150\/ijms.36516","volume":"16","author":"R Tabassum","year":"2019","unstructured":"Tabassum R, Jeong NY (2019) Potential for therapeutic use of hydrogen sulfide in oxidative stress-induced neurodegenerative diseases. Int J Med Sci 16:1386\u20131396","journal-title":"Int J Med Sci"},{"key":"1043_CR34","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1038\/35102174","volume":"414","author":"S Temple","year":"2001","unstructured":"Temple S (2001) The development of neural stem cells. Nature 414:112\u2013117","journal-title":"Nature"},{"key":"1043_CR35","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1089\/ars.2007.9.257","volume":"9","author":"M Tsugane","year":"2007","unstructured":"Tsugane M, Nagai Y, Kimura Y, Oka J, Kimura H (2007) Differentiated astrocytes acquire sensitivity to hydrogen sulfide that is diminished by the transformation into reactive astrocytes. Antioxid Redox Signal 9:257\u2013269","journal-title":"Antioxid Redox Signal"},{"key":"1043_CR36","doi-asserted-by":"publisher","first-page":"1164","DOI":"10.1038\/s41467-017-01283-z","volume":"8","author":"GE Tyzack","year":"2017","unstructured":"Tyzack GE, Hall CE, Sibley CR, Cymes T, Forostyak S, Carlino G, Meyer IF, Schiavo G, Zhang SC, Gibbons GM, Newcombe J, Patani R, Lakatos A (2017) A neuroprotective astrocyte state is induced by neuronal signal EphB1 but fails in ALS models. Nat Commun 8:1164","journal-title":"Nat Commun"},{"key":"1043_CR37","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1111\/j.1468-1331.2009.02752.x","volume":"17","author":"F Valentino","year":"2010","unstructured":"Valentino F, Bivona G, Butera D, Paladino P, Fazzari M, Piccoli T, Ciaccio M, La Bella V (2010) Elevated cerebrospinal fluid and plasma homocysteine levels in ALS. Eur J Neurol 17:84\u201389","journal-title":"Eur J Neurol"},{"key":"1043_CR38","doi-asserted-by":"publisher","first-page":"1585","DOI":"10.1073\/pnas.92.5.1585","volume":"92","author":"M Watanabe","year":"1995","unstructured":"Watanabe M, Osada J, Aratani Y, Kluckman K, Reddick R, Malinow MR, Maeda N (1995) Mice deficient in cystathionine beta-synthase: animal models for mild and severe homocyst(e)inemia. Proc Natl Acad Sci USA 92:1585\u20131589","journal-title":"Proc Natl Acad Sci USA"},{"key":"1043_CR39","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1016\/j.bbrc.2004.11.110","volume":"326","author":"M Whiteman","year":"2005","unstructured":"Whiteman M, Cheung NS, Zhu YZ, Chu SH, Siau JL, Wong BS, Armstrong JS, Moore PK (2005) Hydrogen sulphide: a novel inhibitor of hypochlorous acid-mediated oxidative damage in the brain? Biochem Biophys Res Commun 326:794\u2013798","journal-title":"Biochem Biophys Res Commun"},{"key":"1043_CR40","doi-asserted-by":"publisher","first-page":"1566","DOI":"10.1007\/s00018-008-7440-8","volume":"65","author":"MJ Wieduwilt","year":"2008","unstructured":"Wieduwilt MJ, Moasser MM (2008) The epidermal growth factor receptor family: biology driving targeted therapeutics. Cell Mol Life Sci 65:1566\u20131584","journal-title":"Cell Mol Life Sci"},{"key":"1043_CR41","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1186\/1742-2094-9-202","volume":"9","author":"A Xuan","year":"2012","unstructured":"Xuan A, Long D, Li J, Ji W, Zhang M, Hong L, Liu J (2012) Hydrogen sulfide attenuates spatial memory impairment and hippocampal neuroinflammation in \u03b2-amyloid rat model of Alzheimer\u2019s disease. J Neuroinflammation 9:202","journal-title":"J Neuroinflammation"},{"key":"1043_CR42","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1002\/embr.201338213","volume":"15","author":"K Zhao","year":"2014","unstructured":"Zhao K, Ju Y, Li S, Altaany Z, Wang R, Yang G (2014) S-sulfhydration of MEK1 leads to PARP-1 activation and DNA damage repair. EMBO Rep 15:792\u2013800","journal-title":"EMBO Rep"}],"container-title":["In Vitro Cellular & Developmental Biology - Animal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11626-025-01043-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11626-025-01043-0\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11626-025-01043-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T15:07:52Z","timestamp":1748617672000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11626-025-01043-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4]]},"references-count":42,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4]]}},"alternative-id":["1043"],"URL":"https:\/\/doi.org\/10.1007\/s11626-025-01043-0","relation":{},"ISSN":["1071-2690","1543-706X"],"issn-type":[{"type":"print","value":"1071-2690"},{"type":"electronic","value":"1543-706X"}],"subject":[],"published":{"date-parts":[[2025,4]]},"assertion":[{"value":"9 January 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"23 March 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"15 May 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declared that they do not have anything to disclose regarding funding or conflict of interest with respect to this manuscript.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interest"}}]}}