{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T04:45:09Z","timestamp":1792817109673,"version":"4.5.0"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T00:00:00Z","timestamp":1753833600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K10298"],"award-info":[{"award-number":["24K10298"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K15311"],"award-info":[{"award-number":["23K15311"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009158","name":"Kawasaki Medical School","doi-asserted-by":"publisher","award":["R06B-098"],"award-info":[{"award-number":["R06B-098"]}],"id":[{"id":"10.13039\/501100009158","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016189","name":"Wesco Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100016189","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["In Vitro Cell.Dev.Biol.-Animal"],"published-print":{"date-parts":[[2025,9]]},"DOI":"10.1007\/s11626-025-01074-7","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:00:03Z","timestamp":1753891203000},"page":"956-965","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Establishment of a method to evaluate the dynamics of highly chemotactic THP-1 cells during differentiation into monocyte-M1 macrophage-like cells"],"prefix":"10.1007","volume":"61","author":[{"given":"Shuichiro","family":"Okamoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei","family":"Miyano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasumitsu","family":"Nishimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahoko","family":"Tomonobu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rie","family":"Kinoshita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakiyo","family":"Sakaguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Einosuke","family":"Ikeshita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayasa","family":"Kamezaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Morihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Futoshi","family":"Kuribayashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9205-6922","authenticated-orcid":false,"given":"Akira","family":"Yamauchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,30]]},"reference":[{"issue":"4","key":"1074_CR1","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1111\/j.1600-0765.1999.tb02243.x","volume":"34","author":"AA Baqui","year":"1999","unstructured":"Baqui AA, Meiller TF, Kelley JI, Turng BF, Falkler WA (1999) Antigen activation of THP-1 human monocytic cells after stimulation with lipopolysaccharide from oral microorganisms and granulocyte-macrophage colony-stimulating factor. J Periodontal Res 34(4):203\u2013213. https:\/\/doi.org\/10.1111\/j.1600-0765.1999.tb02243.x","journal-title":"J Periodontal Res"},{"issue":"12","key":"1074_CR2","doi-asserted-by":"publisher","first-page":"4577","DOI":"10.1111\/bcp.14889","volume":"87","author":"AD Bondarev","year":"2021","unstructured":"Bondarev AD, Attwood MM, Attwood MM, Jonsson J, Chubarev VN, Tarasov VV, Schi\u00f6th HB (2021) Recent developments of HDAC inhibitors: emerging indications and novel molecules. Br J Clin Pharmacol 87(12):4577\u20134597. https:\/\/doi.org\/10.1111\/bcp.14889","journal-title":"Br J Clin Pharmacol"},{"key":"1074_CR3","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1084\/jem.115.3.453","volume":"115","author":"S Boyden","year":"1962","unstructured":"Boyden S (1962) The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leucocytes. J Exp Med 115:453\u2013466","journal-title":"J Exp Med"},{"issue":"1","key":"1074_CR4","doi-asserted-by":"publisher","first-page":"e8668","DOI":"10.1371\/journal.pone.0008668","volume":"5","author":"M Daigneault","year":"2010","unstructured":"Daigneault M, Preston JA, Marriott HM, Whyte MKB, Dockrell DH (2010) The identification of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages. PLoS ONE 5(1):e8668. https:\/\/doi.org\/10.1371\/journal.pone.0008668","journal-title":"PLoS ONE"},{"issue":"6124","key":"1074_CR5","doi-asserted-by":"publisher","first-page":"717","DOI":"10.1038\/327717a0","volume":"327","author":"MC Dinauer","year":"1987","unstructured":"Dinauer MC, Orkin SH, Brown R, Jesaitis AJ, Parkos CA (1987) The glycoprotein encoded by the X-linked chronic granulomatous disease locus is a component of the neutrophil cytochrome b complex. Nature 327(6124):717\u2013720. https:\/\/doi.org\/10.1038\/327717a0","journal-title":"Nature"},{"issue":"1","key":"1074_CR6","doi-asserted-by":"publisher","first-page":"9466","DOI":"10.1038\/s41598-018-27710-9","volume":"8","author":"JM Fox","year":"2018","unstructured":"Fox JM, Kausar F, Day A, Osborne M, Hussain K, Mueller A, Lin J, Tsuchiya T, Kanegasaki S, Pease JE (2018) CXCL4\/platelet factor 4 is an agonist of CCR1 and drives human monocyte migration. Sci Rep 8(1):9466. https:\/\/doi.org\/10.1038\/s41598-018-27710-9","journal-title":"Sci Rep"},{"issue":"5","key":"1074_CR7","first-page":"345","volume":"76","author":"H Hemmi","year":"1985","unstructured":"Hemmi H, Breitman TR (1985) Induction of functional differentiation of a human monocytic leukemia cell line (THP-1) by retinoic acid and cholera toxin. Jpn J Cancer Res 76(5):345\u2013351","journal-title":"Jpn J Cancer Res"},{"issue":"1\u20132","key":"1074_CR8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jim.2003.07.008","volume":"282","author":"S Kanegasaki","year":"2003","unstructured":"Kanegasaki S, Nomura Y, Nitta N, Akiyama S, Tamatani T, Goshoh Y, Yoshida T, Sato T, Kikuchi Y (2003) A novel optical assay system for the quantitative measurement of chemotaxis. J Immunol Methods 282(1\u20132):1\u201311. https:\/\/doi.org\/10.1016\/j.jim.2003.07.008","journal-title":"J Immunol Methods"},{"key":"1074_CR9","doi-asserted-by":"publisher","first-page":"113213","DOI":"10.1016\/j.jim.2021.113213","volume":"501","author":"C Kawai","year":"2022","unstructured":"Kawai C, Miyano K, Okamoto S, Yamauchi A, Kuribayashi F (2022) Fine definition of the epitopes on the human gp91\/NOX2 for the monoclonal antibodies CL-5 and 48. J Immunol Methods 501:113213. https:\/\/doi.org\/10.1016\/j.jim.2021.113213","journal-title":"J Immunol Methods"},{"key":"1074_CR10","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1021\/acsmedchemlett.7b00315","volume":"8","author":"N Kindon","year":"2017","unstructured":"Kindon N, Andrews G, Baxter A, Cheshire D, Hemsley P, Johnson T, Liu YZ, McGinnity D, McHale M, Mete A, Reuberson J, Roberts B, Steele J, Teobald B, Unitt J, Vaughan D, Walters I, Stocks MJ (2017) Discovery of AZD-2098 and AZD-1678, two potent and bioavailable CCR4 receptor antagonists. ACS Med Chem Lett 8:981\u2013986. https:\/\/doi.org\/10.1021\/acsmedchemlett.7b00315","journal-title":"ACS Med Chem Lett"},{"issue":"25","key":"1074_CR11","doi-asserted-by":"publisher","first-page":"19000","DOI":"10.1074\/jbc.M001222200","volume":"275","author":"M Liang","year":"2000","unstructured":"Liang M, Mallari C, Rosser M, Ng HP, May K, Monahan S, Bauman JG, Islam I, Ghannam A, Buckman B, Shaw K, Wei GP, Xu W, Zhao Z, Ho E, Shen J, Oanh H, Subramanyam B, Vergona R, Taub D, Dunning L, Harvey S, Snider RM, Hesselgesser J, Morrissey MM, Perez HD (2000) Identification and characterization of a potent, selective, and orally active antagonist of the CC chemokine receptor-1. J Biol Chem 275(25):19000\u201319008. https:\/\/doi.org\/10.1074\/jbc.M001222200","journal-title":"J Biol Chem"},{"issue":"4","key":"1074_CR12","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1016\/j.immuni.2005.10.001","volume":"23","author":"A Mantovani","year":"2005","unstructured":"Mantovani A, Sica A, Locati M (2005) Macrophage polarization comes of age. Immunity 23(4):344\u2013346. https:\/\/doi.org\/10.1016\/j.immuni.2005.10.001","journal-title":"Immunity"},{"issue":"4","key":"1074_CR13","doi-asserted-by":"publisher","first-page":"1485","DOI":"10.1084\/jem.169.4.1485","volume":"169","author":"K Matsushima","year":"1989","unstructured":"Matsushima K, Larsen CG, DuBois GC, Oppenheim JJ (1989) Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line. J Exp Med 169(4):1485. https:\/\/doi.org\/10.1084\/jem.169.4.1485","journal-title":"J Exp Med"},{"key":"1074_CR14","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.diff.2022.10.001","volume":"128","author":"ZN Mohd Yasin","year":"2022","unstructured":"Mohd Yasin ZN, Mohd Idrus FN, Hoe CH, Yvonne-Tee GB (2022) Macrophage polarization in THP-1 cell line and primary monocytes: a systematic review. Differentiation 128:67\u201382. https:\/\/doi.org\/10.1016\/j.diff.2022.10.001","journal-title":"Differentiation"},{"issue":"Pt 1","key":"1074_CR15","doi-asserted-by":"publisher","first-page":"124581","DOI":"10.1016\/j.ijbiomac.2023.124581","volume":"242","author":"M Nandave","year":"2023","unstructured":"Nandave M, Acharjee R, Bhaduri K, Upadhyay J, Rupanagunta GP, Ansari MN (2023) A pharmacological review on SIRT 1 and SIRT 2 proteins, activators, and inhibitors: Call for further research. Int J Biol Macromol 242(Pt 1):124581. https:\/\/doi.org\/10.1016\/j.ijbiomac.2023.124581","journal-title":"Int J Biol Macromol"},{"issue":"1\u20132","key":"1074_CR16","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/j.jim.2006.12.010","volume":"320","author":"N Nitta","year":"2007","unstructured":"Nitta N, Tsuchiya T, Yamauchi A, Tamatani T, Kanegasaki S (2007) Quantitative analysis of eosinophil chemotaxis tracked using a novel optical device \u2013 TAXIScan. J Immunol Methods 320(1\u20132):155\u2013163. https:\/\/doi.org\/10.1016\/j.jim.2006.12.010","journal-title":"J Immunol Methods"},{"issue":"3","key":"1074_CR17","doi-asserted-by":"publisher","first-page":"1018","DOI":"10.4049\/jimmunol.141.3.1018","volume":"141","author":"TJ Schall","year":"1988","unstructured":"Schall TJ, Jongstra J, Dyer BJ, Jorgensen J, Clayberger C, Davis MM, Krensky AK (1988) A human T cell-specific molecule is a member of a new gene family. J Immunol 141(3):1018\u20131025","journal-title":"J Immunol"},{"key":"1074_CR18","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1016\/j.molimm.2017.05.027","volume":"88","author":"H Shiratori","year":"2017","unstructured":"Shiratori H, Feinweber C, Luckhardt S, Linke B, Resch E, Geisslinger G, Weigert A, Parnham MJ (2017) THP-1 and human peripheral blood mononuclear cell-derived macrophages differ in their capacity to polarize in vitro. Mol Immunol 88:58\u201368. https:\/\/doi.org\/10.1016\/j.molimm.2017.05.027","journal-title":"Mol Immunol"},{"issue":"4","key":"1074_CR19","first-page":"1530","volume":"42","author":"S Tsuchiya","year":"1982","unstructured":"Tsuchiya S, Kobayashi Y, Goto Y, Okumura H, Nakae S, Konno T, Tada K (1982) Induction of maturation in cultured human monocytic leukemia cells by a phorbol diester. Cancer Res 42(4):1530\u20131536","journal-title":"Cancer Res"},{"issue":"2","key":"1074_CR20","doi-asserted-by":"publisher","first-page":"171","DOI":"10.1002\/ijc.2910260208","volume":"26","author":"S Tsuchiya","year":"1980","unstructured":"Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K (1980) Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer 26(2):171\u2013176. https:\/\/doi.org\/10.1002\/ijc.2910260208","journal-title":"Int J Cancer"},{"key":"1074_CR21","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1084\/jem.128.3.415","volume":"128","author":"R van Furth","year":"1968","unstructured":"van Furth R, Cohn ZA (1968) The origin and kinetics of mononuclear phagocytes. J Exp Med 128:415\u2013435. https:\/\/doi.org\/10.1084\/jem.128.3.415","journal-title":"J Exp Med"},{"issue":"6","key":"1074_CR22","doi-asserted-by":"publisher","first-page":"2040","DOI":"10.4049\/jimmunol.149.6.2040","volume":"149","author":"E Vey","year":"1992","unstructured":"Vey E, Zhang JH, Dayer JM (1992) IFN-gamma and 1,25(OH)2D3 induce on THP-1 cells distinct patterns of cell surface antigen expression, cytokine production, and responsiveness to contact with activated T cells. J Immunol 149(6):2040\u20132046","journal-title":"J Immunol"},{"issue":"4","key":"1074_CR23","doi-asserted-by":"publisher","first-page":"587","DOI":"10.1124\/dmd.104.002626","volume":"33","author":"DK Walker","year":"2005","unstructured":"Walker DK, Abel S, Comby P, Muirhead GJ, Nedderman AN, Smith DA (2005) Species differences in the disposition of the CCR5 antagonist, UK-427,857, a new potential treatment for HIV. Drug Metab Dispos 33(4):587\u2013595. https:\/\/doi.org\/10.1124\/dmd.104.002626","journal-title":"Drug Metab Dispos"},{"issue":"16","key":"1074_CR24","doi-asserted-by":"publisher","first-page":"14687","DOI":"10.18632\/oncotarget.4189","volume":"6","author":"R Ward","year":"2015","unstructured":"Ward R, Sims AH, Lee A, Lo C, Wynne L, Yusuf H, Gregson H, Lisanti MP, Sotgia F, Landberg G, Lamb R (2015) Monocytes and macrophages, implications for breast cancer migration and stem cell-like activity and treatment. Oncotarget 6(16):14687\u201314699. https:\/\/doi.org\/10.18632\/oncotarget.4189","journal-title":"Oncotarget"},{"issue":"2","key":"1074_CR25","doi-asserted-by":"publisher","first-page":"570","DOI":"10.1084\/jem.167.2.570","volume":"167","author":"SD Wolpe","year":"1988","unstructured":"Wolpe SD, Davatelis G, Sherry B, Beutler B, Hesse DG, Nguyen HT, Moldawer LL, Nathan CF, Lowry SF, Cerami A (1988) Macrophages secrete a novel heparin-binding protein with inflammatory and neutrophil chemokinetic properties. J Exp Med 167(2):570\u2013581. https:\/\/doi.org\/10.1084\/jem.167.2.570","journal-title":"J Exp Med"},{"key":"1074_CR26","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/j.jim.2013.12.005","volume":"404","author":"A Yamauchi","year":"2014","unstructured":"Yamauchi A, Degawa-Yamauchi M, Kuribayashi F, Kanegasaki S, Tsuchiya T (2014) Systematic single cell analysis of migration and morphological changes of human neutrophils over stimulus concentration gradients. J Immunol Methods 404:59\u201370. https:\/\/doi.org\/10.1016\/j.jim.2013.12.005","journal-title":"J Immunol Methods"},{"issue":"1","key":"1074_CR27","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1186\/s12885-017-3218-4","volume":"17","author":"A Yamauchi","year":"2017","unstructured":"Yamauchi A, Yamamura M, Katase N, Itadani M, Okada N, Kobiki K, Nakamura M, Yamaguchi Y, Kuribayashi F (2017) Evaluation of pancreatic cancer cell migration with multiple parameters in vitro by using an optical real-time cell mobility assay device. BMC Cancer 17(1):234. https:\/\/doi.org\/10.1186\/s12885-017-3218-4","journal-title":"BMC Cancer"},{"issue":"3","key":"1074_CR28","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1111\/j.1348-0421.2001.tb02614.x","volume":"45","author":"A Yamauchi","year":"2001","unstructured":"Yamauchi A, Yu L, P\u00f6tgens AJ, Kuribayashi F, Nunoi H, Kanegasaki S, Roos D, Malech HL, Dinauer MC, Nakamura M (2001) Location of the epitope for 7D5, a monoclonal antibody raised against human flavocytochrome b558, to the extracellular peptide portion of primate gp91phox. Microbiol Immunol 45(3):249\u2013257. https:\/\/doi.org\/10.1111\/j.1348-0421.2001.tb02614.x","journal-title":"Microbiol Immunol"},{"issue":"24","key":"1074_CR29","doi-asserted-by":"publisher","first-page":"9233","DOI":"10.1073\/pnas.84.24.9233","volume":"84","author":"T Yoshimura","year":"1987","unstructured":"Yoshimura T, Matsushima K, Tanaka S, Robinson EA, Appella E, Oppenheim JJ, Leonard EJ (1987) Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines. Proc Natl Acad Sci U S A 84(24):9233\u20139237. https:\/\/doi.org\/10.1073\/pnas.84.24.9233","journal-title":"Proc Natl Acad Sci U S A"}],"container-title":["In Vitro Cellular &amp; Developmental Biology - Animal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11626-025-01074-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11626-025-01074-7\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11626-025-01074-7.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T15:08:54Z","timestamp":1762355334000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11626-025-01074-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,30]]},"references-count":29,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,9]]}},"alternative-id":["1074"],"URL":"https:\/\/doi.org\/10.1007\/s11626-025-01074-7","relation":{},"ISSN":["1071-2690","1543-706X"],"issn-type":[{"value":"1071-2690","type":"print"},{"value":"1543-706X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,30]]},"assertion":[{"value":"3 February 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"2 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}