{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T14:57:54Z","timestamp":1780930674457,"version":"3.54.1"},"reference-count":31,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2026JJ81694"],"award-info":[{"award-number":["2026JJ81694"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004735","name":"Natural Science Foundation of Hunan Province","doi-asserted-by":"publisher","award":["2026JJ82507"],"award-info":[{"award-number":["2026JJ82507"]}],"id":[{"id":"10.13039\/501100004735","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["In Vitro Cell.Dev.Biol.-Animal"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Decorin was identified as the most relevant biomarker in type 2 diabetes or obesity-related diseases, but its function in gestational obesity or gestational diabetes mellitus (GDM) remains unknown. RT-qPCR or western blot were employed to validate the expression of Decorin and ADAMTS12 in the umbilical cord blood or placental tissues of the aforementioned groups. Subsequently, an LPS\u2009+\u2009high glucose\u2013induced primary trophoblast cell model was established to verify the expression of DCN and ADAMTS12 in trophoblasts. Based on this model, silencing (si) of Decorin, or Decorin recombinant protein (DCN-r) combined with si-ADAMTS12 intervention, was performed to investigate its effects on trophoblast cell proliferation, migration, invasion, and other characteristics. Compared to the normal pregnancy group, the levels of DCN and ADAMTS12 showed an increasing trend in the gestational obesity group and the GDM group. Notably, DCN and ADAMTS12 were markedly higher in the GDM with obesity group compared to either the gestational obesity or GDM-alone groups. LPS\u2009+\u2009high glucose inhibited the migration and invasion of primary trophoblast cells while promoting increased levels of DCN and ADAMTS12. Under LPS\u2009+\u2009high-glucose intervention, DCN-r further suppressed trophoblast cell migration and invasion while enhancing ADAMTS12 expression, whereas si-DCN reversed these effects. Additionally, si-ADAMTS12 attenuated the LPS\u2009+\u2009high glucose\u2013induced decline in trophoblast cell migration and invasion and blocked the promoting effects of DCN\u00a0recombinant protein. Silencing ADAMTS12 can inhibit the promotive effect of DCN on the migration and invasion capabilities of LPS\u2009+\u2009high glucose\u2013induced primary trophoblast cells. Clinical trial number: not applicable.<\/jats:p>","DOI":"10.1007\/s11626-026-01166-y","type":"journal-article","created":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T17:56:59Z","timestamp":1774375019000},"page":"639-648","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Decorin inhibits the migration and invasion of the LPS\u2009+\u2009high glucose\u2013induced primary trophoblast cell through ADAMTS12"],"prefix":"10.1007","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-5543-2957","authenticated-orcid":false,"given":"Qiuling","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taoyan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,3,24]]},"reference":[{"issue":"11","key":"1166_CR1","doi-asserted-by":"publisher","first-page":"3249","DOI":"10.1093\/humrep\/des297","volume":"27","author":"M Adam","year":"2012","unstructured":"Adam M, Saller S, Str\u00f6bl S, Hennebold JD, Dissen GA, Ojeda SR, Stouffer RL, Berg D, Berg U, Mayerhofer A (2012) Decorin is a part of the ovarian extracellular matrix in primates and may act as a signaling molecule. Hum Reprod 27(11):3249\u20133258","journal-title":"Hum Reprod"},{"issue":"26","key":"1166_CR2","doi-asserted-by":"publisher","first-page":"5231","DOI":"10.1182\/blood-2012-07-442038","volume":"120","author":"A Arning","year":"2012","unstructured":"Arning A, Hiersche M, Witten A, Kurlemann G, Kurnik K, Manner D, Stoll M, Nowak-G\u00f6ttl U (2012) A genome-wide association study identifies a gene network of ADAMTS genes in the predisposition to pediatric stroke. Blood 120(26):5231\u20135236","journal-title":"Blood"},{"issue":"2","key":"1166_CR3","doi-asserted-by":"publisher","first-page":"269","DOI":"10.3171\/2015.7.JNS154","volume":"125","author":"A Arning","year":"2016","unstructured":"Arning A, Jeibmann A, K\u00f6hnemann S, Brokinkel B, Ewelt C, Berger K, Wellmann J, Nowak-G\u00f6ttl U, Stummer W, Stoll M, Holling M (2016) ADAMTS genes and the risk of cerebral aneurysm. J Neurosurg 125(2):269\u2013274","journal-title":"J Neurosurg"},{"issue":"8","key":"1166_CR4","doi-asserted-by":"publisher","first-page":"4846","DOI":"10.1210\/jc.2004-2242","volume":"90","author":"L Belkacemi","year":"2005","unstructured":"Belkacemi L, Lash GE, Macdonald-Goodfellow SK, Caldwell JD, Graham CH (2005) Inhibition of human trophoblast invasiveness by high glucose concentrations. J Clin Endocrinol Metab 90(8):4846\u20134851","journal-title":"J Clin Endocrinol Metab"},{"issue":"1","key":"1166_CR5","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1007\/s12017-012-8169-y","volume":"14","author":"IN Bespalova","year":"2012","unstructured":"Bespalova IN, Angelo GW, Ritter BP, Hunter J, Reyes-Rabanillo ML, Siever LJ, Silverman JM (2012) Genetic variations in the ADAMTS12 gene are associated with schizophrenia in Puerto Rican patients of Spanish descent. Neuromolecular Med 14(1):53\u201364","journal-title":"Neuromolecular Med"},{"issue":"7","key":"1166_CR6","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1038\/ijo.2008.41","volume":"32","author":"K Bolton","year":"2008","unstructured":"Bolton K, Segal D, McMillan J, Jowett J, Heilbronn L, Abberton K, Zimmet P, Chisholm D, Collier G, Walder K (2008) Decorin is a secreted protein associated with obesity and type 2 diabetes. International journal of obesity. 32(7):1113\u201321","journal-title":"International journal of obesity."},{"key":"1166_CR7","first-page":"67","volume-title":"The small leucine-rich proteoglycan, biglycan, is highly expressed in adipose tissue of Psammomys obesus and is associated with obesity and type 2 diabetes","author":"K Bolton","year":"2012","unstructured":"Bolton K, Segal D, Walder K (2012) The small leucine-rich proteoglycan, biglycan, is highly expressed in adipose tissue of Psammomys obesus and is associated with obesity and type 2 diabetes. Targets and Therapy, Biologics, pp 67\u201372"},{"key":"1166_CR8","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2020.479258","volume":"11","author":"F Chen","year":"2020","unstructured":"Chen F, Lai J, Zhu Y, He M, Hou H, Wang J, Chen C, Wang DW, Tang J (2020) Cardioprotective effect of decorin in type 2 diabetes. Front Endocrinol (Lausanne) 11:479258","journal-title":"Front Endocrinol (Lausanne)"},{"key":"1166_CR9","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1016\/j.bbrc.2023.05.117","volume":"671","author":"Q Chen","year":"2023","unstructured":"Chen Q, Hu K, Shi J, Li H, Li W (2023) Hesperidin inhibits methylation and autophagy in LPS and high glucose-induced human villous trophoblasts. Biochem Biophys Res Commun 671:278\u2013285","journal-title":"Biochem Biophys Res Commun"},{"key":"1166_CR10","volume":"2022","author":"Q Chen","year":"2022","unstructured":"Chen Q, Li W, Deng Y, Li Y, Huang L, Zhao L, Li H (2022) Correlation analysis of umbilical cord blood metabolic phenotype and inflammation in patients with gestational diabetes mellitus complicated with overweight and obesity. Evid Based Complement Alternat Med 2022:6072286","journal-title":"Evid Based Complement Alternat Med"},{"issue":"4","key":"1166_CR11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0224251","volume":"15","author":"S Du","year":"2020","unstructured":"Du S, Shao J, Xie D, Zhang F (2020) Decorin inhibits glucose-induced lens epithelial cell apoptosis via suppressing p22phox-p38 MAPK signaling pathway. PLoS One 15(4):e0224251","journal-title":"PLoS One"},{"issue":"3","key":"1166_CR12","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1093\/annonc\/mdw652","volume":"28","author":"TH Ho","year":"2017","unstructured":"Ho TH, Serie DJ, Parasramka M, Cheville JC, Bot BM, Tan W, Wang L, Joseph RW, Hilton T, Leibovich BC, Parker AS (2017) Differential gene expression profiling of matched primary renal cell carcinoma and metastases reveals upregulation of extracellular matrix genes. Annals of Oncology. 28(3):604\u201310","journal-title":"Annals of Oncology."},{"issue":"4","key":"1166_CR13","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1080\/15513815.2023.2197057","volume":"42","author":"I Icen Taskin","year":"2023","unstructured":"Icen Taskin I (2023) Elevated expression of ADAMTS-12 in maternal and umbilical cord serum of placenta accreta spectrum. Fetal Pediatr Pathol 42(4):642\u2013650","journal-title":"Fetal Pediatr Pathol"},{"key":"1166_CR14","doi-asserted-by":"crossref","unstructured":"Jinsheng L, Fuqiong C, Jing C, Guoran R, Mengying H, Chen C, Jiarong T and Wen WD (2017)\"Overexpression of decorin promoted angiogenesis in diabetic cardiomyopathy via IGF1R-AKT-VEGF signaling.\" Scientific reports 7(1).","DOI":"10.1038\/srep44473"},{"issue":"2","key":"1166_CR15","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1111\/jhn.12999","volume":"35","author":"SC Langley-Evans","year":"2022","unstructured":"Langley-Evans SC, Pearce J, Ellis S (2022) Overweight, obesity and excessive weight gain in pregnancy as risk factors for adverse pregnancy outcomes: a narrative review. J Hum Nutr Diet 35(2):250\u2013264","journal-title":"J Hum Nutr Diet"},{"key":"1166_CR16","doi-asserted-by":"publisher","DOI":"10.3390\/biom12070959","author":"T Li","year":"2022","unstructured":"Li T, Peng J, Li Q, Shu Y, Zhu P, Hao L (2022) The mechanism and role of ADAMTS protein family in osteoarthritis. Biomolecules. https:\/\/doi.org\/10.3390\/biom12070959","journal-title":"Biomolecules"},{"issue":"7","key":"1166_CR17","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.92941","volume":"3","author":"TJ Mead","year":"2018","unstructured":"Mead TJ, McCulloch DR, Ho JC, Du Y, Adams SM, Birk DE, Apte SS (2018) The metalloproteinase-proteoglycans ADAMTS7 and ADAMTS12 provide an innate, tendon-specific protective mechanism against heterotopic ossification. JCI insight. 3(7):e92941","journal-title":"JCI insight."},{"key":"1166_CR18","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.ejogrb.2024.02.008","volume":"295","author":"J Nian","year":"2024","unstructured":"Nian J, Zhu Y, Lv X, Zhang Y, Xue Z, Wu Z, Zhou Y, Liu Y (2024) Expression levels of ADAMTS 5, 9, and 12 in endometrial polyps and their predictive value for the diagnosis and recurrence of endometrial polyps. Eur J Obstet Gynecol Reprod Biol 295:86\u201391","journal-title":"Eur J Obstet Gynecol Reprod Biol"},{"key":"1166_CR19","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.mehy.2014.06.020","volume":"104","author":"S Ozler","year":"2017","unstructured":"Ozler S, Demircan K (2017) The investigation of the role of proteoglycans and ADAMTS levels in fetal membranes in physiopathological process of gestational diabetes. Med Hypotheses 104:182\u2013184","journal-title":"Med Hypotheses"},{"issue":"2","key":"1166_CR20","doi-asserted-by":"publisher","first-page":"e78","DOI":"10.1542\/neo.22-2-e78","volume":"22","author":"C Paredes","year":"2021","unstructured":"Paredes C, Hsu RC, Tong A, Johnson JR (2021) Obesity and pregnancy. NeoReviews 22(2):e78\u2013e87","journal-title":"NeoReviews"},{"issue":"6","key":"1166_CR21","doi-asserted-by":"publisher","first-page":"2265","DOI":"10.1007\/s13300-019-00713-z","volume":"10","author":"DF Sarina","year":"2019","unstructured":"Sarina DF, Li ZQ, Feng J, Du WH, Zhao N, Huang JC, Jia ZY, Wu A, Wang YY, Ji XL, Yu L (2019) Mechanism of placenta damage in gestational diabetes mellitus by investigating TXNIP of patient samples and gene functional research in cell line. Diabetes Ther 10(6):2265\u20132288","journal-title":"Diabetes Ther"},{"issue":"1","key":"1166_CR22","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.bpobgyn.2010.10.006","volume":"25","author":"D Simmons","year":"2011","unstructured":"Simmons D (2011) Diabetes and obesity in pregnancy. Best practice & research Clinical obstetrics & gynaecology. 25(1):25\u201336","journal-title":"Best practice & research Clinical obstetrics & gynaecology."},{"issue":"7","key":"1166_CR23","doi-asserted-by":"publisher","first-page":"2698","DOI":"10.1080\/01443615.2022.2099253","volume":"42","author":"N Soobryan","year":"2022","unstructured":"Soobryan N, Kumar A, Moodley J, Mackraj I (2022) An observational study of pro- and anti-angiogenic factors in hypertensive disorders of pregnancy in women of African ancestry. J Obstet Gynaecol 42(7):2698\u20132703","journal-title":"J Obstet Gynaecol"},{"key":"1166_CR24","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-37501-x","author":"J Sv\u00e4rd","year":"2019","unstructured":"Sv\u00e4rd J, R\u00f8st TH, Sommervoll CEN, Haugen C, Gudbrandsen OA, Mellgren AE, R\u00f8dahl E, Fern\u00f8 J, Dankel SN, Sagen JV, Mellgren G (2019) Absence of the proteoglycan decorin reduces glucose tolerance in overfed male mice. Sci Rep. https:\/\/doi.org\/10.1038\/s41598-018-37501-x","journal-title":"Sci Rep"},{"issue":"7","key":"1166_CR25","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1139\/apnm-2018-0667","volume":"44","author":"CP Talbot","year":"2019","unstructured":"Talbot CP, Dolinsky VW (2019) Sex differences in the developmental origins of cardiometabolic disease following exposure to maternal obesity and gestational diabetes. Applied Physiology, Nutrition, and Metabolism. 44(7):687\u201395","journal-title":"Applied Physiology, Nutrition, and Metabolism."},{"key":"1166_CR26","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1016\/j.matbio.2022.12.005","volume":"115","author":"M Urbanczyk","year":"2023","unstructured":"Urbanczyk M, Jeyagaran A, Zbinden A, Lu CE, Marzi J, Kuhlburger L, Nahnsen S, Layland SL, Duffy G, Schenke-Layland K (2023) Decorin improves human pancreatic \u03b2-cell function and regulates ECM expression in vitro. Matrix Biol 115:160\u2013183","journal-title":"Matrix Biol"},{"key":"1166_CR27","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/649718","volume":"2014","author":"J Wei","year":"2014","unstructured":"Wei J, Richbourgh B, Jia T, Liu C (2014) ADAMTS-12: a multifaced metalloproteinase in arthritis and inflammation. Mediators Inflamm 2014:649718","journal-title":"Mediators Inflamm"},{"issue":"8","key":"1166_CR28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0237928","volume":"15","author":"A Witten","year":"2020","unstructured":"Witten A, R\u00fchle F, de Witt M, Barysenka A, Stach M, Junker R, Nowak-G\u00f6ttl U, Stoll M (2020) ADAMTS12, a new candidate gene for pediatric stroke. PLoS One 15(8):e0237928","journal-title":"PLoS One"},{"issue":"2","key":"1166_CR29","first-page":"233","volume":"18","author":"S Yamachika","year":"2000","unstructured":"Yamachika S, Brayer J, Oxford GE, Peck AB, Humphreysbeher MG (2000) Aberrant proteolytic digestion of biglycan and decorin by saliva and exocrine gland lysates from the NOD mouse model for autoimmune exocrinopathy. Clin Exp Rheumatol 18(2):233","journal-title":"Clin Exp Rheumatol"},{"issue":"1","key":"1166_CR30","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-023-00661-2","volume":"29","author":"S Yang","year":"2023","unstructured":"Yang S, Zhou X, Jia Z, Zhang M, Yuan M, Zhou Y, Wang J, Xia D (2023) Epigenetic regulatory mechanism of ADAMTS12 expression in osteoarthritis. Mol Med 29(1):86","journal-title":"Mol Med"},{"issue":"5","key":"1166_CR31","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000028738","volume":"101","author":"L Zhao","year":"2022","unstructured":"Zhao L, Wu T, Li J, Cai C, Yao Q, Zhu YS (2022) Data-independent acquisition-based proteomics analysis correlating type 2 diabetes mellitus with osteoarthritis in total knee arthroplasty patients. Medicine (Baltimore) 101(5):e28738","journal-title":"Medicine (Baltimore)"}],"container-title":["In Vitro Cellular &amp; Developmental Biology - Animal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11626-026-01166-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11626-026-01166-y","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11626-026-01166-y.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T14:00:18Z","timestamp":1780927218000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11626-026-01166-y"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,3,24]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["1166"],"URL":"https:\/\/doi.org\/10.1007\/s11626-026-01166-y","relation":{},"ISSN":["1071-2690","1543-706X"],"issn-type":[{"value":"1071-2690","type":"print"},{"value":"1543-706X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,3,24]]},"assertion":[{"value":"6 November 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 January 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 March 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study was approved by Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control, Changsha Hospital for Maternal & Child Health Care Affiliated to Hunan Normal University (EC-20240828-19), Changsha, China.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics approval and consent to participate"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}