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However, the limited availability of stable muscle progenitor cell lines from commercially important species such as Atlantic salmon (\n                    <jats:italic>Salmo salar<\/jats:italic>\n                    ) constrains mechanistic studies and emerging applications in cellular aquaculture. Here, we report the establishment and characterization of a novel embryonic-derived salmon muscle progenitor cell line, termed SsEC. These cells were derived from late embryonic stages and exhibited a spindle-shaped morphology, robust proliferative capacity, and sustained expansion beyond 30 passages under defined culture conditions. SsECs demonstrated a distinct extracellular matrix preference, with vitronectin supporting long-term maintenance and expansion. Molecular characterization confirmed stable expression of canonical myogenic markers, including\u00a0\n                    <jats:italic>myf5<\/jats:italic>\n                    \u00a0and\u00a0\n                    <jats:italic>myod1<\/jats:italic>\n                    , while transcriptomic profiling revealed enrichment of genes associated with muscle development and sarcomere organization relative to a non-myogenic salmon cell line. Directed differentiation to muscle, using a two-step protocol, induced efficient formation of multinucleated myotubes expressing myosin heavy chain and sarcomeric \u03b1-actinin, with upregulation of key differentiation markers such as\u00a0\n                    <jats:italic>myog<\/jats:italic>\n                    \u00a0and\u00a0\n                    <jats:italic>tnnt3a<\/jats:italic>\n                    . Together, these findings establish SsECs as a robust in vitro model cell line for studying salmon muscle development and provide a novel platform for applications in aquaculture research and cellular seafood production.\n                  <\/jats:p>","DOI":"10.1007\/s11626-026-01221-8","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T18:17:17Z","timestamp":1788286637000},"page":"1026-1040","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Derivation and characterization of an embryonic\u2011derived muscle progenitor cell line from Atlantic salmon (Salmo salar)"],"prefix":"10.1007","volume":"62","author":[{"given":"Kyle","family":"Naylor","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia","family":"Webb","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deepika","family":"Rajesh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick J.","family":"Mee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"key":"1221_CR1","unstructured":"ATCC (2026) ASK [Atlantic salmon kidney] (CRL\u20112747). 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Embryos were handled in accordance with institutional and national guidelines for the use of fish embryos in research. All work was conducted in accordance with institutional and national guidelines governing the use of fish embryos and did not require regulated animal procedures.","order":1,"name":"Ethics","label":"Ethics statement","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"All authors are employees of Roslin Technologies Ltd. The authors declare no competing interests.","order":2,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}}]}}