{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T22:00:43Z","timestamp":1790114443785,"version":"4.0.1"},"reference-count":37,"publisher":"Springer Science and Business Media LLC","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Chin. J. Integr. Med."],"DOI":"10.1007\/s11655-026-3867-8","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T08:40:29Z","timestamp":1790066429000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Liangfu Pill Alleviates Constipation-Predominant Irritable Bowel Syndrome through Modulation of 5-HT Signaling Pathway and Gut Microbiota"],"prefix":"10.1007","author":[{"given":"Shui-di","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie-ying","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bai-rong","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun-ping","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan-song","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao-miao","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao-yan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan-fen","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"3867_CR1","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1007\/s00108-021-01218-3","volume":"63","author":"L Gottschalk","year":"2022","unstructured":"Gottschalk L, Andresen V, Layer P. Irritable bowel syndrome and functional dyspepsia: diagnosis and treatment. Der Internist 2022;63:51\u201361.","journal-title":"Der Internist"},{"key":"3867_CR2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41395-018-0084-x","volume":"113","author":"AC Ford","year":"2018","unstructured":"Ford AC, Moayyedi P, Chey WD, Harris LA, Lacy BE, Saito YA, et al. American College of Gastroenterology Monograph on Management of Irritable Bowel Syndrome. Am J Gastroenterol 2018;113:1\u201318.","journal-title":"Am J Gastroenterol"},{"key":"3867_CR3","first-page":"24","volume":"33","author":"L Chu","year":"2019","unstructured":"Chu L, Tang H. Therapeutic effect of Chaihu Shugan Powder on constipation-predominant irritable bowel syndrome. J Pract Tradit Chin Intern Med (Chin) 2019;33:24\u201326.","journal-title":"J Pract Tradit Chin Intern Med (Chin)"},{"key":"3867_CR4","first-page":"74","volume":"20","author":"X Guo","year":"2020","unstructured":"Guo X. Application of Baizhong Yiqi Decoction in constipation-type irritable bowel syndrome. Prac Clin J Integr Tradit Chin West Med (Chin) 2020;20:74\u201375,108.","journal-title":"Prac Clin J Integr Tradit Chin West Med (Chin)"},{"key":"3867_CR5","first-page":"1063","volume":"32","author":"Q Wei","year":"2021","unstructured":"Wei Q, Liang S, Jiang S, Wei N. Study on network pharmacology mechanism of Liangfu Pills in the treatment of gastric ulcer. Chin Pharm (Chin) 2021;32:1063\u20131069.","journal-title":"Chin Pharm (Chin)"},{"key":"3867_CR6","first-page":"198","volume":"42","author":"F Wu","year":"2024","unstructured":"Wu F, Peng S, Wen Z, Chen D, Feng Z. Clinical efficacy of Liangfu Pills in common gastric diseases using meta analysis. J Guangdong Med Univ (Chin) 2024;42:198\u2013202,226.","journal-title":"J Guangdong Med Univ (Chin)"},{"key":"3867_CR7","first-page":"3853","volume":"47","author":"J He","year":"2022","unstructured":"He J, Gui B, Chen Y, Yin Y, Tao S, Shen Z, et al. Mechanism of Liangfu Pills in treatment of functional dyspepsia: based on network pharmacology and experimental verification. China J Chin Mater Med (Chin) 2022;47: 3853\u20133862.","journal-title":"China J Chin Mater Med (Chin)"},{"key":"3867_CR8","first-page":"28","volume":"21","author":"X Ma","year":"2026","unstructured":"Ma X, Bai Y. The impacts of galangin on gut microbiota and intestinal mucosal barrier in rats with acute pancreatitis by adjusting SIRT1\/PGC-1 \u03b1 pathway. J Pathog Biol 2026;21:28\u201333.","journal-title":"J Pathog Biol"},{"key":"3867_CR9","first-page":"937","volume":"26","author":"J Wang","year":"2022","unstructured":"Wang J, Hu Y, Yang L. Study on the correlation between serum zonulin and monocyte chemoattractant protein-1 levels in patients with irritable bowel syndrome and the intestinal flora, inflammatory reaction and severity of gastrointestinal symptoms. Anhui Med Pharm J (Chin) 2022;26: 937\u2013941.","journal-title":"Anhui Med Pharm J (Chin)"},{"key":"3867_CR10","doi-asserted-by":"publisher","first-page":"100195","DOI":"10.1016\/j.fochx.2021.100195","volume":"13","author":"JJ Rodr\u00edguez-Romero","year":"2022","unstructured":"Rodr\u00edguez-Romero JJ, Dur\u00e1n-Casta\u00f1eda AC, C\u00e1rdenas-Castro AP, S\u00e1nchez-Burgos JA, Zamora-Gasga VM, S\u00e1yago-Ayerdi SG. What we know about protein gut metabolites: implications and insights for human health and diseases. Food Chem X 2022;13:100195.","journal-title":"Food Chem X"},{"key":"3867_CR11","first-page":"1206","volume":"44","author":"Y Yao","year":"2024","unstructured":"Yao Y, Xu L, Yang J, Hu D, Yang P, Xu S. Investigation of the mechanism by which Zhizhu Decoction improves intestinal transport function in rats with functional constipation based on gut microbiota structural characteristics and 5-HT. Chin J Gerontol (Chin) 2024;44:1206\u20131209.","journal-title":"Chin J Gerontol (Chin)"},{"key":"3867_CR12","doi-asserted-by":"publisher","first-page":"1395","DOI":"10.1096\/fj.14-259598","volume":"29","author":"CS Reigstad","year":"2015","unstructured":"Reigstad CS, Salmonson CE, Rainey JF3rd, Szurszewski JH, Linden DR, Sonnenburg JL, et al. Gut microbes promote colonic serotonin production through an effect of short-chain fatty acids on enterochromaffin cells. FASEB J 2015;29:1395\u20131403.","journal-title":"FASEB J"},{"key":"3867_CR13","doi-asserted-by":"crossref","unstructured":"Andoh A, Miwa H. Association between the gut microbiota and the pathophysiology of irritable bowel syndrome\u2014 a narrative review. Digestion 2026 [Epub ahead of print]. DOI:10.11591000550907.","DOI":"10.1159\/000550907"},{"key":"3867_CR14","doi-asserted-by":"publisher","first-page":"4851","DOI":"10.1002\/j.2040-4603.2023.tb00272.x","volume":"13","author":"SA Najjar","year":"2023","unstructured":"Najjar SA, Hung LY, Margolis KG. Serotonergic control of gastrointestinal development, motility, and inflammation. Compr Physiol 2023;13:4851\u20134868.","journal-title":"Compr Physiol"},{"key":"3867_CR15","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1016\/j.ejphar.2018.02.005","volume":"826","author":"Y Mine","year":"2018","unstructured":"Mine Y, Itakura T, Oku S, Asada R, Shimizu I. DSP-6952, a novel 5-HT4 receptor partial agonist, inhibits visceral hypersensitivity and ameliorates gastrointestinal dysfunction in experimental animals. Eur J Pharmacol 2018;826:123\u2013132.","journal-title":"Eur J Pharmacol"},{"key":"3867_CR16","first-page":"1555","volume":"31","author":"JH He","year":"2023","unstructured":"He JH, He JY, Zhang BR, Zhang SD, Wei YJ, Yang CY, et al. Establishment and evaluation of constipated irritable bowel syndrome model with liver depression syndrome. Acta Lab Anim Sci Sin 2023;31:1555\u20131563.","journal-title":"Acta Lab Anim Sci Sin"},{"key":"3867_CR17","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.3390\/foods12051101","volume":"12","author":"L Wu","year":"2023","unstructured":"Wu L, Gao L, Jin X, Chen Z, Qiao X, Cui X, et al. Ethanol extract of Mao Jian Green Tea attenuates gastrointestinal symptoms in a rat model of irritable bowel syndrome with constipation via the 5-hydroxytryptamine signaling pathway. Foods 2023;12:1101.","journal-title":"Foods"},{"key":"3867_CR18","first-page":"1324","volume":"41","author":"J Fu","year":"2022","unstructured":"Fu J, Gui B, Li K, Yang C, Wei L, Liang J, et al. Pharmacodynamic study of Liangfu Pill in the treatment of functional dyspepsia of stomach-cold type. Herald Med 2022;41:1324\u20131330.","journal-title":"Herald Med"},{"key":"3867_CR19","doi-asserted-by":"publisher","first-page":"461","DOI":"10.33549\/physiolres.935245","volume":"73","author":"L Gajdo\u0161ov\u00e1","year":"2024","unstructured":"Gajdo\u0161ov\u00e1 L, Katren\u010d\u00edkov\u00e1 B, Borb\u00e9lyov\u00e1 V, Muchov\u00e1 J. The effect of omega-3 fatty acid supplementation and exercise on locomotor activity, exploratory activity, and anxiety-like behavior in adult and aged rats. Physiol Res 2024;73:461\u2013480.","journal-title":"Physiol Res"},{"key":"3867_CR20","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1007\/s00706-022-02924-w","volume":"153","author":"D Nov\u00e1kov\u00e1","year":"2022","unstructured":"Nov\u00e1kov\u00e1 D, Kudl\u00e1\u010dek K, Novotn\u00fd J, Nesm\u0115r\u00e1k K. Improvement of conditions for the determination of neurotransmitters in rat brain tissue by HPLC with fluorimetric detection. Monatsh Chem 2022;153:753\u2013758.","journal-title":"Monatsh Chem"},{"key":"3867_CR21","doi-asserted-by":"publisher","first-page":"7730","DOI":"10.1080\/10408398.2021.1917508","volume":"62","author":"L Chen","year":"2022","unstructured":"Chen L, Cao H, Huang Q, Xiao J, Teng H. Absorption, metabolism and bioavailability of flavonoids: a review. Crit Rev Food Sci Nutr 2022;62:7730\u20137742.","journal-title":"Crit Rev Food Sci Nutr"},{"key":"3867_CR22","doi-asserted-by":"publisher","first-page":"132531","DOI":"10.1016\/j.foodchem.2022.132531","volume":"383","author":"N Shen","year":"2022","unstructured":"Shen N, Wang T, Gan Q, Liu S, Wang L, Jin B. Plant flavonoids: classification, distribution, biosynthesis, and antioxidant activity. Food Chem 2022;383:132531.","journal-title":"Food Chem"},{"key":"3867_CR23","first-page":"6461","volume":"37","author":"D Xin","year":"2022","unstructured":"Xin D, Liang X, Teng J, Zhang Y, Ma X, Tang Y. Effect and mechanism of couplet medicine of Aurantii Fructus Immaturus\u2013Paeoniae Radix Alba on regulation of C-IBS based on VIP-cAMP-PKA-AQP3 signaling pathway. China J Tradit Chin Med Pharm (Chin) 2022;37:6461\u20136465.","journal-title":"China J Tradit Chin Med Pharm (Chin)"},{"key":"3867_CR24","first-page":"4849","volume":"24","author":"H Zhang","year":"2022","unstructured":"Zhang H, Li X, Li L, Zhou G, Zhou X, Gong A, et al. Effects of thermal moxibustion on SP and CGRP expression in serum and colon tissue of IBS model rats. Mod Tradit Chin Med Mater Med World Sci Technol (Chin) 2022;24:4849\u20134854.","journal-title":"Mod Tradit Chin Med Mater Med World Sci Technol (Chin)"},{"key":"3867_CR25","doi-asserted-by":"publisher","first-page":"948","DOI":"10.1590\/S0100-879X2012007500122","volume":"45","author":"C Yan","year":"2012","unstructured":"Yan C, Liu XG, Wang HH, Li JX, Li YX. Effect of the 5-HT4 receptor and serotonin transporter on visceral hypersensitivity in rats. Braz J Med Biol Res 2012:45:948\u2013954.","journal-title":"Braz J Med Biol Res"},{"key":"3867_CR26","series-title":"dissertation","volume-title":"Research about the mechanism of IBS-C treated with electroacupuncture based on 5-HT signaling system","author":"L Pei","year":"2017","unstructured":"Pei L. Research about the mechanism of IBS-C treated with electroacupuncture based on 5-HT signaling system [dissertation]. Nanjing: Nanjing University of Chinese Medicine; 2017."},{"key":"3867_CR27","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/S0091-3057(01)00746-8","volume":"71","author":"D Hoyer","year":"2002","unstructured":"Hoyer D, Hannon JP, Martin GR. Molecular, pharmacological and functional diversity of 5-HT receptors. Pharmacol Biochem Behav 2002;71:533\u2013554.","journal-title":"Pharmacol Biochem Behav"},{"key":"3867_CR28","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/j.ejphar.2014.03.061","volume":"735","author":"MJ Lee","year":"2014","unstructured":"Lee MJ, Cho KH, Park HM, Sung HJ, Choi S, Im W. Pharmacological profile of DA-6886, a novel 5-HT4 receptor agonist to accelerate colonic motor activity in mice. Eur J Pharmacol 2014;735:115\u2013122.","journal-title":"Eur J Pharmacol"},{"key":"3867_CR29","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s12328-017-0813-5","volume":"11","author":"A Nishida","year":"2018","unstructured":"Nishida A, Inoue R, Inatomi O, Bamba S, Naito Y, Andoh A. Gut microbiota in the pathogenesis of inflammatory bowel disease. Clin J Gastroenterol 2018;11:1\u201310.","journal-title":"Clin J Gastroenterol"},{"key":"3867_CR30","first-page":"764","volume":"37","author":"E Zhao","year":"2021","unstructured":"Zhao E, Zhou J, Chu H, Lv S, Yang Y. Epidemiology and pathogenic factors of irritable bowel syndrome: a review on research progress. Chin J Public Health (Chin) 2021;37:764\u2013768.","journal-title":"Chin J Public Health (Chin)"},{"key":"3867_CR31","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1152\/physrev.00005.2019","volume":"100","author":"K Talavera","year":"2020","unstructured":"Talavera K, Startek JB, Alvarez-Collazo J, Boonen B, Alpizar YA, Sanchez A, et al. Mammalian transient receptor potential TRPA1 channels: from structure to disease. Physiol Rev 2020;100:725\u2013803.","journal-title":"Physiol Rev"},{"key":"3867_CR32","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.jcmgh.2020.08.002","volume":"11","author":"MA Engevik","year":"2021","unstructured":"Engevik MA, Luck B, Visuthranukul C, Ihekweazu FD, Engevik AC, Shi Z, et al. Human-derived Bifidobacterium dentium modulates the mammalian serotonergic system and gut\u2013brain axis. Cell Mol Gastroenterol Hepatol 2021;11:221\u2013248.","journal-title":"Cell Mol Gastroenterol Hepatol"},{"key":"3867_CR33","doi-asserted-by":"publisher","first-page":"130344","DOI":"10.1016\/j.bbagen.2023.130344","volume":"1867","author":"S Kumei","year":"2023","unstructured":"Kumei S, Ishioh M, Nozu T, Okumura T. Prostaglandin I2 suppresses the development of gut\u2013brain axis disorder in irritable bowel syndrome in rats. Biochim Biophys Acta Gen Sub 2023;1867:130344.","journal-title":"Biochim Biophys Acta Gen Sub"},{"key":"3867_CR34","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1128\/CMR.00014-14","volume":"28","author":"JC Lagier","year":"2015","unstructured":"Lagier JC, Hugon P, Khelaifia S, Fournier PE, La Scola B, Raoult D. The rebirth of culture in microbiology through the example of culturomics to study human gut microbiota. Clin Microbiol Rev 2015;28:237\u2013264.","journal-title":"Clin Microbiol Rev"},{"key":"3867_CR35","first-page":"7","volume":"39","author":"W Feng","year":"2019","unstructured":"Feng W, Wu H. Mechanism of the treatment of irritable bowel syndrome by traditional Chinese medicine based on the short chain fatty acid regulating 5-HT. Jilin J Chin Med (Chin) 2019;39:7\u20139,23.","journal-title":"Jilin J Chin Med (Chin)"},{"key":"3867_CR36","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1038\/s41575-019-0157-3","volume":"16","author":"B Dalile","year":"2019","unstructured":"Dalile B, Van Oudenhove L, Vervliet B, Verbeke K. The role of short-chain fatty acids in microbiota\u2013gut\u2013brain communication. Nat Rev Gastroenterol Hepatol 2019;16:461\u2013478.","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"3867_CR37","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1093\/jpp\/rgad120","volume":"76","author":"M Zhang","year":"2024","unstructured":"Zhang M, Dang M, Li Y, Wei P, Zhao C, Dong T. Natural products derived from herbs as promising source for diarrhea predominant-irritable bowel syndrome via gut microbiota\u2013serotonin pathway. J Pharm Pharmacol 2024;76:75\u201385.","journal-title":"J Pharm Pharmacol"}],"container-title":["Chinese Journal of Integrative Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11655-026-3867-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11655-026-3867-8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11655-026-3867-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T21:03:36Z","timestamp":1790111016000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11655-026-3867-8"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,22]]},"references-count":37,"alternative-id":["3867"],"URL":"https:\/\/doi.org\/10.1007\/s11655-026-3867-8","relation":{},"ISSN":["1672-0415","1993-0402"],"issn-type":[{"value":"1672-0415","type":"print"},{"value":"1993-0402","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,22]]},"assertion":[{"value":"29 April 2026","order":1,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 September 2026","order":2,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}