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PCSA of the vastus lateralis increased and ascent of stairs time decreased after 6 months of increased serum 25(OH)D.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Purpose<\/jats:title>\n                <jats:p>The Institute of Medicine recommends \u2265 20 ng\/ml of serum 25-hydroxyvitamin D [25(OH)D] for bone and overall health. Serum 25(OH)D levels have been associated with physical performance, postural sway, and falls. The purpose of this study was to determine if increasing postmenopausal women\u2019s serum 25(OH)D levels from 20\u201330 ng\/ml to 40\u201350 ng\/ml improved skeletal muscle size, strength, balance, and functional performance while decreasing skeletal muscle fatigue.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Twenty-six post-menopausal women (60\u201385 years old) with baseline serum 25(OH)D levels between 20 and 30 ng\/ml were recruited. Oral over-the-counter (OTC) vitamin D3 and calcium citrate were prescribed to increase subjects\u2019 serum 25(OH)D to levels between 40 and 50 ng\/ml, serum calcium levels above 9.2 mg\/dl, and PTH levels below 60 pg\/ml, which were confirmed at 6 and 12 weeks. Outcome measures assessed at baseline and 6 months included muscle physiological cross-sectional area (PCSA), muscle strength, postural balance, time to perform functional tasks, and muscle fatigue. Repeated measures comparisons between baseline and follow-up were performed.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>Nineteen subjects completed the study. One individual could not afford the time commitment for the repeated measures. Three individuals did not take their vitamin D as recommended. Two subjects were lost to follow-up (lack of interest), and one did not achieve targeted serum 25(OH)D. Vastus lateralis PCSA increased (<jats:italic>p<\/jats:italic> = 0.007) and ascent of stair time decreased (<jats:italic>p<\/jats:italic> = 0.042) after 6 months of increasing serum 25(OH)D levels from 20\u201330 ng\/ml to 40\u201350 ng\/ml. Isometric strength was unchanged. Anterior-posterior center of pressure (COP) excursion and COP path length decreased (<jats:italic>p<\/jats:italic> &lt; 0.1) albeit non-significantly, suggesting balance may improve from increased serum 25(OH)D and calcium citrate levels.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusions<\/jats:title>\n                <jats:p>Several measures of muscle structure and function were sensitive to elevated serum 25(OH)D and calcium levels indicating that further investigation of this phenomenon in post-menopausal women is warranted.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s11657-020-00814-4","type":"journal-article","created":{"date-parts":[[2020,10,3]],"date-time":"2020-10-03T14:03:04Z","timestamp":1601733784000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Effect of increased serum 25(OH)D and calcium on structure and function of post-menopausal women: a pilot study"],"prefix":"10.1007","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6440-1917","authenticated-orcid":false,"given":"H. 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