{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T12:39:33Z","timestamp":1763037573966,"version":"3.37.3"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T00:00:00Z","timestamp":1591920000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T00:00:00Z","timestamp":1591920000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","award":["APQ-00827-08"],"award-info":[{"award-number":["APQ-00827-08"]}],"id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Parasit."],"published-print":{"date-parts":[[2020,12]]},"DOI":"10.1007\/s11686-020-00235-w","type":"journal-article","created":{"date-parts":[[2020,12,15]],"date-time":"2020-12-15T16:04:13Z","timestamp":1608048253000},"page":"995-998","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of Different Oocyst DNA Extraction Methods for Cryptosporidium spp. Research in Environmental Samples"],"prefix":"10.1007","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9211-7008","authenticated-orcid":false,"given":"Neliane Cristina","family":"Moreira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3288-3974","authenticated-orcid":false,"given":"Marlene","family":"Cabrine-Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4853-1559","authenticated-orcid":false,"given":"M\u00e1rcia Benedita","family":"de Oliveira-Silva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2020,6,12]]},"reference":[{"key":"235_CR1","doi-asserted-by":"publisher","first-page":"119","DOI":"10.5216\/rpt.v41i2.19320","volume":"41","author":"RMB Franco","year":"2012","unstructured":"Franco RMB, Branco N, Leal DAG (2012) Parasitologia ambiental: m\u00e9todos de concentra\u00e7\u00e3o e detec\u00e7\u00e3o de Cryptosporidium spp. Giardia spp. em amostras de \u00e1gua (Environmental parasitology: methods of concentration and detection of Cryptosporidium spp. and Giardia spp. in water samples). Rev Patol Trop 41:119\u2013135. https:\/\/doi.org\/10.5216\/rpt.v41i2.19320","journal-title":"Rev Patol Trop"},{"key":"235_CR2","doi-asserted-by":"publisher","first-page":"818","DOI":"10.1016\/j.watres.2003.10.012","volume":"38","author":"CM Carey","year":"2004","unstructured":"Carey CM, Lee H, Trevors JT (2004) Biology, persistence and detection of Cryptosporidium parvum and Cryptosporidium hominis oocyst. Water Res 38:818\u2013862. https:\/\/doi.org\/10.1016\/j.watres.2003.10.012","journal-title":"Water Res"},{"key":"235_CR3","doi-asserted-by":"publisher","first-page":"4446","DOI":"10.1128\/AEM.71.8.4446-4454.2005","volume":"71","author":"J Jiang","year":"2005","unstructured":"Jiang J, Alderisio KA, Xiao L (2005) Distribuition of Cryptosporidium genotypes in storm event water samples from three watersheds in New York. Appl Environ Microbiol 71:4446\u20134454. https:\/\/doi.org\/10.1128\/AEM.71.8.4446-4454.2005","journal-title":"Appl Environ Microbiol"},{"key":"235_CR4","doi-asserted-by":"publisher","first-page":"839","DOI":"10.2307\/3285819","volume":"85","author":"JR Harris","year":"1999","unstructured":"Harris JR, Petry F (1999) Cryptosporidium parvum: structural components of the oocyst wall. J Parasitol 85:839\u2013849. https:\/\/doi.org\/10.2307\/3285819","journal-title":"J Parasitol"},{"key":"235_CR5","doi-asserted-by":"publisher","first-page":"495","DOI":"10.1128\/JCM.28.3.495-503.1990","volume":"28","author":"R Boom","year":"1990","unstructured":"Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, Van der Noordaa J (1990) Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28:495\u2013503","journal-title":"J Clin Microbiol"},{"key":"235_CR6","doi-asserted-by":"publisher","first-page":"3153","DOI":"10.1128\/JCM.37.10.3153-3158.1999","volume":"37","author":"J McLauchlin","year":"1999","unstructured":"McLauchlin J, Pedraza-D\u00edaz S, Amar-Hoetzeneder C, Nichols GL (1999) Genetic characterization of Cryptosporidium strains from 218 patients with diarrhea diagnosed as having sporadic cryptosporidiosis. J Clin Microbiol 37:3153\u20133158","journal-title":"J Clin Microbiol"},{"key":"235_CR7","doi-asserted-by":"publisher","first-page":"1769","DOI":"10.1128\/JCM.34.7.1769-1772.1996","volume":"34","author":"AB Balatbat","year":"1996","unstructured":"Balatbat AB, Jordan GW, Tang YJ, Silva J (1996) Detection of Cryptosporidium parvum DNA in human feces by nested PCR. J Clin Microbiol 34:1769\u20131772","journal-title":"J Clin Microbiol"},{"key":"235_CR8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0304-4017(95)00863-2","volume":"62","author":"X Leng","year":"1996","unstructured":"Leng X, Mosier DA, Oberst RD (1996) Differentiation of Cryptosporidium parvum, C. muris, and C. baileyi by PCR-RFLP analysis of the 18s rRNA gene. Vet Parasitol 62:1\u20137. https:\/\/doi.org\/10.1016\/0304-4017(95)00863-2","journal-title":"Vet Parasitol"},{"key":"235_CR9","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1186\/s13104-018-3300-2","volume":"11","author":"E Menu","year":"2018","unstructured":"Menu E, Mary C, Toga I, Raoult D, Ranque S, Bittar F (2018) Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples. BMC Res Notes 11:2\u20136","journal-title":"BMC Res Notes"},{"key":"235_CR10","doi-asserted-by":"publisher","first-page":"1045","DOI":"10.1007\/s00436-011-2342-3","volume":"109","author":"H Yoshikawa","year":"2011","unstructured":"Yoshikawa H, Dogruman AF, Turk S, Kustimur S, Balaban NS (2011) Evaluation of DNA extraction kits for molecular diagnosis of human Blastocystis subtypes from fecal samples. Parasitol Res 109:1045\u20131050. https:\/\/doi.org\/10.1007\/s00436-011-2342-3","journal-title":"Parasitol Res"},{"key":"235_CR11","doi-asserted-by":"publisher","first-page":"512","DOI":"10.1590\/S0037-86822007000500004","volume":"40","author":"MB Oliveira-Silva","year":"2007","unstructured":"Oliveira-Silva MB, Oliveira LR, Resende JCP, Peghini BC, Ramirez LE, Lages-Silva E, Correia D (2007) Seasonal profile and level of CD4+ lymphocytes in the occurrence of cryptosporidiosis and cystoisosporidiosis in HIV\/AIDS patients in the Tri\u00e2ngulo Mineiro region, Brazil. Rev Soc Bras Med Trop 40:512\u2013515. https:\/\/doi.org\/10.1590\/S0037-86822007000500004","journal-title":"Rev Soc Bras Med Trop"},{"key":"235_CR12","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1016\/s0169-4758(00)01699-9","volume":"16","author":"L Xiao","year":"2000","unstructured":"Xiao L, Morgan UM, Fayer R, Thompson RC, Lal AA (2000) Cryptosporidium systematics and implications for public health. Parasitol Today 16:287\u2013292. https:\/\/doi.org\/10.1016\/s0169-4758(00)01699-9","journal-title":"Parasitol Today"},{"key":"235_CR13","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1590\/S0036-46652001000200011","volume":"43","author":"RMB Franco","year":"2001","unstructured":"Franco RMB, Rocha-Eberhardt R, Cantusio Neto R (2001) Occurrence of Cryptosporidium oocysts and Giardia cysts in raw water from the Atibaia river, Campinas, Brazil. Rev Inst Med Trop S\u00e3o Paulo 43:109\u2013111. https:\/\/doi.org\/10.1590\/S0036-46652001000200011","journal-title":"Rev Inst Med Trop S\u00e3o Paulo"},{"key":"235_CR14","doi-asserted-by":"publisher","first-page":"1926","DOI":"10.1128\/AEM.02295-09","volume":"76","author":"M Jenkins","year":"2010","unstructured":"Jenkins M, Eaglesham BS, Anthony LC, Ghiorse WC (2010) Significance of wall structure, macromolecular composition, and surface polymers to the survival and transport of Cryptosporidium parvum oocysts. Appl Environ Microbiol 76:1926\u20131934. https:\/\/doi.org\/10.1128\/AEM.02295-09","journal-title":"Appl Environ Microbiol"},{"key":"235_CR15","doi-asserted-by":"publisher","first-page":"263","DOI":"10.3347\/kjp.2014.52.3.263","volume":"52","author":"Y Hawash","year":"2014","unstructured":"Hawash Y (2014) DNA extration from protozoan oocysts\/cysts in feces for diagnostic PCR. Korean J Parasitol 52:263\u2013271. https:\/\/doi.org\/10.3347\/kjp.2014.52.3.263","journal-title":"Korean J Parasitol"},{"key":"235_CR16","doi-asserted-by":"publisher","first-page":"4","DOI":"10.4322\/2357-9730.59345","volume":"36","author":"PHE Weiss","year":"2016","unstructured":"Weiss PHE, Quadros RM, Milletti LC, Marques SMT (2016) Compara\u00e7\u00e3o de seis m\u00e9todos de extra\u00e7\u00e3o de DNA gen\u00f4mico de Giardia duodenalis (Comparison of six methods of extracting genomic DNA from Giardia duodenalis). Clin Biomed Res 36:4\u201310","journal-title":"Clin Biomed Res"}],"container-title":["Acta Parasitologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11686-020-00235-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11686-020-00235-w\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11686-020-00235-w.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,6,11]],"date-time":"2021-06-11T23:50:20Z","timestamp":1623455420000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11686-020-00235-w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,6,12]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["235"],"URL":"https:\/\/doi.org\/10.1007\/s11686-020-00235-w","relation":{},"ISSN":["1230-2821","1896-1851"],"issn-type":[{"type":"print","value":"1230-2821"},{"type":"electronic","value":"1896-1851"}],"subject":[],"published":{"date-parts":[[2020,6,12]]},"assertion":[{"value":"10 January 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 May 2020","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"12 June 2020","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with Ethical Standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}