{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T05:32:50Z","timestamp":1789795970983,"version":"4.0.1"},"reference-count":51,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2021,5,21]],"date-time":"2021-05-21T00:00:00Z","timestamp":1621555200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2021,5,21]],"date-time":"2021-05-21T00:00:00Z","timestamp":1621555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"funder":[{"name":"Natural Science Foundation of Shanghai Municipal Health Commission","award":["20184Y0087"],"award-info":[{"award-number":["20184Y0087"]}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"crossref","award":["2017-QN-05"],"award-info":[{"award-number":["2017-QN-05"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"crossref"}]},{"name":"oundation of faculty development of 100 teachers of Shanghai University of Medicine & Health Sciences","award":["B1-0200-19-311006"],"award-info":[{"award-number":["B1-0200-19-311006"]}]},{"name":"Training Program for Young Scholar of Jiading District Central Hospital","award":["ZQN201804"],"award-info":[{"award-number":["ZQN201804"]}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Parasit."],"published-print":{"date-parts":[[2021,12]]},"DOI":"10.1007\/s11686-021-00415-2","type":"journal-article","created":{"date-parts":[[2021,5,21]],"date-time":"2021-05-21T04:02:43Z","timestamp":1621569763000},"page":"1387-1395","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A Novel Combined DNA Vaccine Encoding Toxoplasma gondii SAG1 and ROP18 Provokes Protective Immunity Against a Lethal Challenge in Mice"],"prefix":"10.1007","volume":"66","author":[{"given":"Lamei","family":"Wu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianglin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuwen","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4175-7117","authenticated-orcid":false,"given":"Yanhong","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenxia","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,5,21]]},"reference":[{"issue":"5","key":"415_CR1","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1891\/0730-0832.34.5.274","volume":"34","author":"MM Hampton","year":"2015","unstructured":"Hampton MM (2015) Congenital Toxoplasmosis: A Review. Neonatal Netw 34(5):274\u2013278. https:\/\/doi.org\/10.1891\/0730-0832.34.5.274","journal-title":"Neonatal Netw"},{"issue":"2","key":"415_CR2","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1128\/CMR.05013-11","volume":"25","author":"F Robert-Gangneux","year":"2012","unstructured":"Robert-Gangneux F, Darde ML (2012) Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev 25(2):264\u2013296. https:\/\/doi.org\/10.1128\/CMR.05013-11","journal-title":"Clin Microbiol Rev"},{"issue":"4","key":"415_CR3","first-page":"160","volume":"57","author":"V Nissapatorn","year":"2004","unstructured":"Nissapatorn V, Lee C, Quek KF, Leong CL, Mahmud R, Abdullah KA (2004) Toxoplasmosis in HIV\/AIDS patients: a current situation. Jpn J Infect Dis 57(4):160\u2013165","journal-title":"Jpn J Infect Dis"},{"issue":"3","key":"415_CR4","doi-asserted-by":"publisher","first-page":"e190","DOI":"10.1371\/journal.pntd.0000190","volume":"2","author":"EJ Mui","year":"2008","unstructured":"Mui EJ, Schiehser GA, Milhous WK, Hsu H, Roberts CW, Kirisits M, Muench S, Rice D, Dubey JP, Fowble JW, Rathod PK, Queener SF, Liu SR, Jacobus DP, McLeod R (2008) Novel triazine JPC-2067-B inhibits Toxoplasma gondii in vitro and in vivo. PLoS Negl Trop Dis 2(3):e190. https:\/\/doi.org\/10.1371\/journal.pntd.0000190","journal-title":"PLoS Negl Trop Dis"},{"issue":"6","key":"415_CR5","doi-asserted-by":"publisher","first-page":"942","DOI":"10.3201\/eid1206.060025","volume":"12","author":"A Khan","year":"2006","unstructured":"Khan A, Jordan C, Muccioli C, Vallochi AL, Rizzo LV, Belfort R Jr, Vitor RW, Silveira C, Sibley LD (2006) Genetic divergence of Toxoplasma gondii strains associated with ocular toxoplasmosis. Brazil Emerg Infect Dis 12(6):942\u2013949. https:\/\/doi.org\/10.3201\/eid1206.060025","journal-title":"Brazil Emerg Infect Dis"},{"issue":"11","key":"415_CR6","doi-asserted-by":"publisher","first-page":"1546","DOI":"10.1136\/bjo.2009.162412","volume":"93","author":"J Bottos","year":"2009","unstructured":"Bottos J, Miller RH, Belfort RN, Macedo AC, Belfort R Jr, Grigg ME (2009) Bilateral retinochoroiditis caused by an atypical strain of Toxoplasma gondii. Br J Ophthalmol 93(11):1546\u20131550. https:\/\/doi.org\/10.1136\/bjo.2009.162412","journal-title":"Br J Ophthalmol"},{"issue":"3","key":"415_CR7","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1586\/14760584.4.3.381","volume":"4","author":"MR Schleiss","year":"2005","unstructured":"Schleiss MR, Heineman TC (2005) Progress toward an elusive goal: current status of cytomegalovirus vaccines. Expert Rev Vaccines 4(3):381\u2013406. https:\/\/doi.org\/10.1586\/14760584.4.3.381","journal-title":"Expert Rev Vaccines"},{"issue":"11","key":"415_CR8","doi-asserted-by":"publisher","first-page":"1287","DOI":"10.1586\/14760584.2013.844652","volume":"12","author":"NZ Zhang","year":"2013","unstructured":"Zhang NZ, Chen J, Wang M, Petersen E, Zhu XQ (2013) Vaccines against Toxoplasma gondii: new developments and perspectives. Expert Rev Vaccines 12(11):1287\u20131299. https:\/\/doi.org\/10.1586\/14760584.2013.844652","journal-title":"Expert Rev Vaccines"},{"issue":"8","key":"415_CR9","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1016\/j.vaccine.2007.11.058","volume":"26","author":"E Jongert","year":"2008","unstructured":"Jongert E, Melkebeek V, De Craeye S, Dewit J, Verhelst D, Cox E (2008) An enhanced GRA1-GRA7 cocktail DNA vaccine primes anti-Toxoplasma immune responses in pigs. Vaccine 26(8):1025\u20131031. https:\/\/doi.org\/10.1016\/j.vaccine.2007.11.058","journal-title":"Vaccine"},{"issue":"1\u20132","key":"415_CR10","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1016\/j.vetpar.2011.12.007","volume":"187","author":"R Fang","year":"2012","unstructured":"Fang R, Feng H, Hu M, Khan MK, Wang L, Zhou Y, Zhao J (2012) Evaluation of immune responses induced by SAG1 and MIC3 vaccine cocktails against Toxoplasma gondii. Vet Parasitol 187(1\u20132):140\u2013146. https:\/\/doi.org\/10.1016\/j.vetpar.2011.12.007","journal-title":"Vet Parasitol"},{"issue":"6","key":"415_CR11","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1586\/erv.09.27","volume":"8","author":"J Kur","year":"2009","unstructured":"Kur J, Holec-Gasior L, Hiszczynska-Sawicka E (2009) Current status of toxoplasmosis vaccine development. Expert Rev Vaccines 8(6):791\u2013808. https:\/\/doi.org\/10.1586\/erv.09.27","journal-title":"Expert Rev Vaccines"},{"issue":"23","key":"415_CR12","doi-asserted-by":"publisher","first-page":"3977","DOI":"10.1016\/j.vaccine.2010.03.028","volume":"28","author":"TG Tan","year":"2010","unstructured":"Tan TG, Mui E, Cong H, Witola WH, Montpetit A, Muench SP, Sidney J, Alexander J, Sette A, Grigg ME, Maewal A, McLeod R (2010) Identification of T. gondii epitopes, adjuvants, and host genetic factors that influence protection of mice and humans. Vaccine 28(23):3977\u20133989. https:\/\/doi.org\/10.1016\/j.vaccine.2010.03.028","journal-title":"Vaccine"},{"issue":"1","key":"415_CR13","doi-asserted-by":"crossref","first-page":"443","DOI":"10.4049\/jimmunol.132.1.443","volume":"132","author":"LH Kasper","year":"1984","unstructured":"Kasper LH, Bradley MS, Pfefferkorn ER (1984) Identification of stage-specific sporozoite antigens of Toxoplasma gondii by monoclonal antibodies. J Immunol 132(1):443\u2013449","journal-title":"J Immunol"},{"issue":"12","key":"415_CR14","doi-asserted-by":"publisher","first-page":"6358","DOI":"10.1128\/IAI.67.12.6358-6363.1999","volume":"67","author":"HV Nielsen","year":"1999","unstructured":"Nielsen HV, Lauemoller SL, Christiansen L, Buus S, Fomsgaard A, Petersen E (1999) Complete protection against lethal Toxoplasma gondii infection in mice immunized with a plasmid encoding the SAG1 gene. Infect Immun 67(12):6358\u20136363","journal-title":"Infect Immun"},{"issue":"1","key":"415_CR15","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1186\/s13643-019-1067-8","volume":"8","author":"JE Feliciano-Alfonso","year":"2019","unstructured":"Feliciano-Alfonso JE, Vargas-Villanueva A, Marin MA, Trivino L, Carvajal N, Moreno M, Luna T, de Mesa CL, Munoz-Ortiz J, de-la Torre A (2019) Antibiotic treatment for ocular toxoplasmosis: a systematic review and meta-analysis: study protocol. Syst Rev 8(1):146. https:\/\/doi.org\/10.1186\/s13643-019-1067-8","journal-title":"Syst Rev"},{"key":"415_CR16","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1186\/1756-3305-7-180","volume":"7","author":"Y Wang","year":"2014","unstructured":"Wang Y, Yin H (2014) Research progress on surface antigen 1 (SAG1) of Toxoplasma gondii. Parasit Vectors 7:180. https:\/\/doi.org\/10.1186\/1756-3305-7-180","journal-title":"Parasit Vectors"},{"issue":"5","key":"415_CR17","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1111\/j.1600-0854.2008.00728.x","volume":"9","author":"MF Cesbron-Delauw","year":"2008","unstructured":"Cesbron-Delauw MF, Gendrin C, Travier L, Ruffiot P, Mercier C (2008) Apicomplexa in mammalian cells: trafficking to the parasitophorous vacuole. Traffic 9(5):657\u2013664. https:\/\/doi.org\/10.1111\/j.1600-0854.2008.00728.x","journal-title":"Traffic"},{"issue":"2","key":"415_CR18","doi-asserted-by":"publisher","first-page":"e16","DOI":"10.1371\/journal.ppat.0030016","volume":"3","author":"AP Sinai","year":"2007","unstructured":"Sinai AP (2007) The toxoplasma kinase ROP18: an active member of a degenerate family. PLoS Pathog 3(2):e16. https:\/\/doi.org\/10.1371\/journal.ppat.0030016","journal-title":"PLoS Pathog"},{"issue":"1","key":"415_CR19","doi-asserted-by":"publisher","first-page":"184","DOI":"10.4161\/hv.26703","volume":"10","author":"Q Liu","year":"2014","unstructured":"Liu Q, Wang F, Wang G, Zhao Q, Min J, Wang S, Cong H, Li Y, He S, Zhou H (2014) Toxoplasma gondii: immune response and protective efficacy induced by ROP16\/GRA7 multicomponent DNA vaccine with a genetic adjuvant B7\u20132. Hum Vaccin Immunother 10(1):184\u2013191. https:\/\/doi.org\/10.4161\/hv.26703","journal-title":"Hum Vaccin Immunother"},{"issue":"12","key":"415_CR20","doi-asserted-by":"publisher","first-page":"1916","DOI":"10.1128\/CVI.00397-12","volume":"19","author":"PY Wang","year":"2012","unstructured":"Wang PY, Yuan ZG, Petersen E, Li J, Zhang XX, Li XZ, Li HX, Lv ZC, Cheng T, Ren D, Yang GL, Lin RQ, Zhu XQ (2012) Protective efficacy of a Toxoplasma gondii rhoptry protein 13 plasmid DNA vaccine in mice. Clin Vaccine Immunol 19(12):1916\u20131920. https:\/\/doi.org\/10.1128\/CVI.00397-12","journal-title":"Clin Vaccine Immunol"},{"issue":"38","key":"415_CR21","doi-asserted-by":"publisher","first-page":"6614","DOI":"10.1016\/j.vaccine.2011.06.110","volume":"29","author":"ZG Yuan","year":"2011","unstructured":"Yuan ZG, Zhang XX, Lin RQ, Petersen E, He S, Yu M, He XH, Zhou DH, He Y, Li HX, Liao M, Zhu XQ (2011) Protective effect against toxoplasmosis in mice induced by DNA immunization with gene encoding Toxoplasma gondii ROP18. Vaccine 29(38):6614\u20136619. https:\/\/doi.org\/10.1016\/j.vaccine.2011.06.110","journal-title":"Vaccine"},{"issue":"6","key":"415_CR22","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1016\/j.chom.2010.11.005","volume":"8","author":"SJ Fentress","year":"2010","unstructured":"Fentress SJ, Behnke MS, Dunay IR, Mashayekhi M, Rommereim LM, Fox BA, Bzik DJ, Taylor GA, Turk BE, Lichti CF, Townsend RR, Qiu W, Hui R, Beatty WL, Sibley LD (2010) Phosphorylation of immunity-related GTPases by a Toxoplasma gondii-secreted kinase promotes macrophage survival and virulence. Cell Host Microbe 8(6):484\u2013495. https:\/\/doi.org\/10.1016\/j.chom.2010.11.005","journal-title":"Cell Host Microbe"},{"issue":"5806","key":"415_CR23","doi-asserted-by":"publisher","first-page":"1780","DOI":"10.1126\/science.1133690","volume":"314","author":"JP Saeij","year":"2006","unstructured":"Saeij JP, Boyle JP, Coller S, Taylor S, Sibley LD, Brooke-Powell ET, Ajioka JW, Boothroyd JC (2006) Polymorphic secreted kinases are key virulence factors in toxoplasmosis. Science 314(5806):1780\u20131783. https:\/\/doi.org\/10.1126\/science.1133690","journal-title":"Science"},{"key":"415_CR24","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.exppara.2014.02.016","volume":"139","author":"J Chen","year":"2014","unstructured":"Chen J, Zhou DH, Li ZY, Petersen E, Huang SY, Song HQ, Zhu XQ (2014) Toxoplasma gondii: protective immunity induced by rhoptry protein 9 (TgROP9) against acute toxoplasmosis. Exp Parasitol 139:42\u201348. https:\/\/doi.org\/10.1016\/j.exppara.2014.02.016","journal-title":"Exp Parasitol"},{"issue":"23","key":"415_CR25","doi-asserted-by":"publisher","first-page":"E5344","DOI":"10.1073\/pnas.1801118115","volume":"115","author":"R An","year":"2018","unstructured":"An R, Tang Y, Chen L, Cai H, Lai DH, Liu K, Wan L, Gong L, Yu L, Luo Q, Shen J, Lun ZR, Ayala FJ, Du J (2018) Encephalitis is mediated by ROP18 of Toxoplasma gondii, a severe pathogen in AIDS patients. Proc Natl Acad Sci USA 115(23):E5344\u2013E5352. https:\/\/doi.org\/10.1073\/pnas.1801118115","journal-title":"Proc Natl Acad Sci USA"},{"issue":"5806","key":"415_CR26","doi-asserted-by":"publisher","first-page":"1776","DOI":"10.1126\/science.1133643","volume":"314","author":"S Taylor","year":"2006","unstructured":"Taylor S, Barragan A, Su C, Fux B, Fentress SJ, Tang K, Beatty WL, Hajj HE, Jerome M, Behnke MS, White M, Wootton JC, Sibley LD (2006) A secreted serine-threonine kinase determines virulence in the eukaryotic pathogen Toxoplasma gondii. Science 314(5806):1776\u20131780. https:\/\/doi.org\/10.1126\/science.1133643","journal-title":"Science"},{"issue":"12","key":"415_CR27","doi-asserted-by":"publisher","first-page":"1609","DOI":"10.1586\/14760584.2015.1098539","volume":"14","author":"NZ Zhang","year":"2015","unstructured":"Zhang NZ, Wang M, Xu Y, Petersen E, Zhu XQ (2015) Recent advances in developing vaccines against Toxoplasma gondii: an update. Expert Rev Vaccines 14(12):1609\u20131621. https:\/\/doi.org\/10.1586\/14760584.2015.1098539","journal-title":"Expert Rev Vaccines"},{"issue":"10","key":"415_CR28","doi-asserted-by":"publisher","first-page":"488","DOI":"10.1016\/j.pt.2004.07.009","volume":"20","author":"J Vercruysse","year":"2004","unstructured":"Vercruysse J, Knox DP, Schetters TP, Willadsen P (2004) Veterinary parasitic vaccines: pitfalls and future directions. Trends Parasitol 20(10):488\u2013492. https:\/\/doi.org\/10.1016\/j.pt.2004.07.009","journal-title":"Trends Parasitol"},{"issue":"5","key":"415_CR29","doi-asserted-by":"publisher","first-page":"549","DOI":"10.3892\/ijmm.4.5.549","volume":"4","author":"HL Robinson","year":"1999","unstructured":"Robinson HL (1999) DNA vaccines: basic mechanism and immune responses (Review). Int J Mol Med 4(5):549\u2013555. https:\/\/doi.org\/10.3892\/ijmm.4.5.549","journal-title":"Int J Mol Med"},{"issue":"4","key":"415_CR30","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1016\/s0952-7915(00)00118-7","volume":"12","author":"S Gurunathan","year":"2000","unstructured":"Gurunathan S, Wu CY, Freidag BL, Seder RA (2000) DNA vaccines: a key for inducing long-term cellular immunity. Curr Opin Immunol 12(4):442\u2013447. https:\/\/doi.org\/10.1016\/s0952-7915(00)00118-7","journal-title":"Curr Opin Immunol"},{"key":"415_CR31","doi-asserted-by":"publisher","first-page":"808","DOI":"10.3389\/fmicb.2016.00808","volume":"7","author":"P Sonaimuthu","year":"2016","unstructured":"Sonaimuthu P, Ching XT, Fong MY, Kalyanasundaram R, Lau YL (2016) Induction of protective immunity against Toxoplasmosis in BALB\/c mice vaccinated with toxoplasma gondii Rhoptry-1. Front Microbiol 7:808. https:\/\/doi.org\/10.3389\/fmicb.2016.00808","journal-title":"Front Microbiol"},{"key":"415_CR32","doi-asserted-by":"publisher","first-page":"59","DOI":"10.3389\/fcimb.2016.00059","volume":"6","author":"LM Bastos","year":"2016","unstructured":"Bastos LM, Macedo AG Jr, Silva MV, Santiago FM, Ramos EL, Santos FA, Pirovani CP, Goulart LR, Mineo TW, Mineo JR (2016) Toxoplasma gondii-derived synthetic peptides containing B- and T-cell epitopes from GRA2 protein are able to enhance mice survival in a model of experimental toxoplasmosis. Front Cell Infect Microbiol 6:59. https:\/\/doi.org\/10.3389\/fcimb.2016.00059","journal-title":"Front Cell Infect Microbiol"},{"key":"415_CR33","doi-asserted-by":"publisher","first-page":"730","DOI":"10.3389\/fimmu.2017.00730","volume":"8","author":"JL Wang","year":"2017","unstructured":"Wang JL, Elsheikha HM, Zhu WN, Chen K, Li TT, Yue DM, Zhang XX, Huang SY, Zhu XQ (2017) Immunization with Toxoplasma gondii GRA17 deletion mutant induces partial protection and survival in challenged mice. Front Immunol 8:730. https:\/\/doi.org\/10.3389\/fimmu.2017.00730","journal-title":"Front Immunol"},{"issue":"25","key":"415_CR34","doi-asserted-by":"publisher","first-page":"3058","DOI":"10.1016\/j.vaccine.2014.03.042","volume":"32","author":"ZY Li","year":"2014","unstructured":"Li ZY, Chen J, Petersen E, Zhou DH, Huang SY, Song HQ, Zhu XQ (2014) Synergy of mIL-21 and mIL-15 in enhancing DNA vaccine efficacy against acute and chronic Toxoplasma gondii infection in mice. Vaccine 32(25):3058\u20133065. https:\/\/doi.org\/10.1016\/j.vaccine.2014.03.042","journal-title":"Vaccine"},{"issue":"8","key":"415_CR35","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1016\/s0264-410x(96)00249-6","volume":"15","author":"HL Robinson","year":"1997","unstructured":"Robinson HL (1997) Nucleic acid vaccines: an overview. Vaccine 15(8):785\u2013787. https:\/\/doi.org\/10.1016\/s0264-410x(96)00249-6","journal-title":"Vaccine"},{"key":"415_CR36","doi-asserted-by":"publisher","first-page":"927","DOI":"10.1146\/annurev.immunol.18.1.927","volume":"18","author":"S Gurunathan","year":"2000","unstructured":"Gurunathan S, Klinman DM, Seder RA (2000) DNA vaccines: immunology, application, and optimization*. Annu Rev Immunol 18:927\u2013974. https:\/\/doi.org\/10.1146\/annurev.immunol.18.1.927","journal-title":"Annu Rev Immunol"},{"issue":"5","key":"415_CR37","doi-asserted-by":"publisher","first-page":"505","DOI":"10.3347\/kjp.2017.55.5.505","volume":"55","author":"Y Han","year":"2017","unstructured":"Han Y, Zhou A, Lu G, Zhao G, Sha W, Wang L, Guo J, Zhou J, Zhou H, Cong H, He S (2017) DNA vaccines encoding Toxoplasma gondii Cathepsin C 1 induce protection against toxoplasmosis in mice. Korean J Parasitol 55(5):505\u2013512. https:\/\/doi.org\/10.3347\/kjp.2017.55.5.505","journal-title":"Korean J Parasitol"},{"key":"415_CR38","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/j.exppara.2018.04.003","volume":"188","author":"Y Chen","year":"2018","unstructured":"Chen Y, Yu M, Hemandez JA, Li J, Yuan ZG, Yan H (2018) Immuno-efficacy of DNA vaccines encoding PLP1 and ROP18 against experimental Toxoplasma gondii infection in mice. Exp Parasitol 188:73\u201378. https:\/\/doi.org\/10.1016\/j.exppara.2018.04.003","journal-title":"Exp Parasitol"},{"key":"415_CR39","doi-asserted-by":"publisher","first-page":"259","DOI":"10.17179\/excli2018-1993","volume":"18","author":"M Foroutan","year":"2019","unstructured":"Foroutan M, Zaki L, Tavakoli S, Soltani S, Taghipour A, Ghaffarifar F (2019) Rhomboid antigens are promising targets in the vaccine development against Toxoplasma gondii. EXCLI J 18:259\u2013272. https:\/\/doi.org\/10.17179\/excli2018-1993","journal-title":"EXCLI J"},{"issue":"7","key":"415_CR40","doi-asserted-by":"publisher","first-page":"10782","DOI":"10.1002\/jcp.27747","volume":"234","author":"P Alizadeh","year":"2019","unstructured":"Alizadeh P, Ahmadpour E, Daryani A, Kazemi T, Spotin A, Mahami-Oskouei M, Flynn RJ, Azadi Y, Rajabi S, Sandoghchian S (2019) IL-17 and IL-22 elicited by a DNA vaccine encoding ROP13 associated with protection against Toxoplasma gondii in BALB\/c mice. J Cell Physiol 234(7):10782\u201310788. https:\/\/doi.org\/10.1002\/jcp.27747","journal-title":"J Cell Physiol"},{"issue":"10","key":"415_CR41","doi-asserted-by":"publisher","first-page":"e12578","DOI":"10.1111\/pim.12578","volume":"40","author":"G Lu","year":"2018","unstructured":"Lu G, Zhou J, Zhao YH, Wang L (2018) DNA vaccine ROP29 from Toxoplasma gondii containing R848 enhances protective immunity in mice. Parasite Immunol 40(10):e12578. https:\/\/doi.org\/10.1111\/pim.12578","journal-title":"Parasite Immunol"},{"issue":"1","key":"415_CR42","doi-asserted-by":"publisher","first-page":"1474","DOI":"10.1038\/s41598-018-37671-8","volume":"9","author":"C Ramakrishnan","year":"2019","unstructured":"Ramakrishnan C, Maier S, Walker RA, Rehrauer H, Joekel DE, Winiger RR, Basso WU, Grigg ME, Hehl AB, Deplazes P, Smith NC (2019) An experimental genetically attenuated live vaccine to prevent transmission of Toxoplasma gondii by cats. Sci Rep 9(1):1474. https:\/\/doi.org\/10.1038\/s41598-018-37671-8","journal-title":"Sci Rep"},{"key":"415_CR43","doi-asserted-by":"publisher","first-page":"592","DOI":"10.3389\/fimmu.2019.00592","volume":"10","author":"J Guo","year":"2019","unstructured":"Guo J, Zhou A, Sun X, Sha W, Ai K, Pan G, Zhou C, Zhou H, Cong H, He S (2019) Immunogenicity of a virus-like-particle vaccine containing multiple antigenic epitopes of Toxoplasma gondii against acute and chronic toxoplasmosis in mice. Front Immunol 10:592. https:\/\/doi.org\/10.3389\/fimmu.2019.00592","journal-title":"Front Immunol"},{"issue":"7","key":"415_CR44","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1038\/emboj.2009.24","volume":"28","author":"W Qiu","year":"2009","unstructured":"Qiu W, Wernimont A, Tang K, Taylor S, Lunin V, Schapira M, Fentress S, Hui R, Sibley LD (2009) Novel structural and regulatory features of rhoptry secretory kinases in Toxoplasma gondii. EMBO J 28(7):969\u2013979. https:\/\/doi.org\/10.1038\/emboj.2009.24","journal-title":"EMBO J"},{"issue":"13\u201314","key":"415_CR45","doi-asserted-by":"publisher","first-page":"1327","DOI":"10.1016\/s0264-410x(02)00692-8","volume":"21","author":"A Fachado","year":"2003","unstructured":"Fachado A, Rodriguez A, Angel SO, Pinto DC, Vila I, Acosta A, Amendoeira RR, Lannes-Vieira J (2003) Protective effect of a naked DNA vaccine cocktail against lethal toxoplasmosis in mice. Vaccine 21(13\u201314):1327\u20131335. https:\/\/doi.org\/10.1016\/s0264-410x(02)00692-8","journal-title":"Vaccine"},{"issue":"2","key":"415_CR46","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1007\/s00436-007-0465-3","volume":"101","author":"J Zhang","year":"2007","unstructured":"Zhang J, He S, Jiang H, Yang T, Cong H, Zhou H, Gu Q, Li Y, Zhao Q (2007) Evaluation of the immune response induced by multiantigenic DNA vaccine encoding SAG1 and ROP2 of Toxoplasma gondii and the adjuvant properties of murine interleukin-12 plasmid in BALB\/c mice. Parasitol Res 101(2):331\u2013338. https:\/\/doi.org\/10.1007\/s00436-007-0465-3","journal-title":"Parasitol Res"},{"issue":"1\u20132","key":"415_CR47","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1111\/j.1365-3024.2005.00738.x","volume":"27","author":"H Cong","year":"2005","unstructured":"Cong H, Gu QM, Jiang Y, He SY, Zhou HY, Yang TT, Li Y, Zhao QL (2005) Oral immunization with a live recombinant attenuated Salmonella typhimurium protects mice against Toxoplasma gondii. Parasite Immunol 27(1\u20132):29\u201335. https:\/\/doi.org\/10.1111\/j.1365-3024.2005.00738.x","journal-title":"Parasite Immunol"},{"issue":"4","key":"415_CR48","doi-asserted-by":"publisher","first-page":"e1004047","DOI":"10.1371\/journal.ppat.1004047","volume":"10","author":"CD Dupont","year":"2014","unstructured":"Dupont CD, Christian DA, Selleck EM, Pepper M, Leney-Greene M, Harms Pritchard G, Koshy AA, Wagage S, Reuter MA, Sibley LD, Betts MR, Hunter CA (2014) Parasite fate and involvement of infected cells in the induction of CD4+ and CD8+ T cell responses to Toxoplasma gondii. PLoS Pathog 10(4):e1004047. https:\/\/doi.org\/10.1371\/journal.ppat.1004047","journal-title":"PLoS Pathog"},{"issue":"2","key":"415_CR49","doi-asserted-by":"publisher","first-page":"187","DOI":"10.1016\/S0020-7519(00)00159-4","volume":"31","author":"GI Solano Aguilar","year":"2001","unstructured":"Solano Aguilar GI, Beshah E, Vengroski KG, Zarlenga D, Jauregui L, Cosio M, Douglass LW, Dubey JP, Lunney JK (2001) Cytokine and lymphocyte profiles in miniature swine after oral infection with Toxoplasma gondii oocysts. Int J Parasitol 31(2):187\u2013195","journal-title":"Int J Parasitol"},{"issue":"1","key":"415_CR50","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1017\/S0031182011001673","volume":"139","author":"JP Dubey","year":"2012","unstructured":"Dubey JP, Ferreira LR, Martins J, McLeod R (2012) Oral oocyst-induced mouse model of toxoplasmosis: effect of infection with Toxoplasma gondii strains of different genotypes, dose, and mouse strains (transgenic, out-bred, in-bred) on pathogenesis and mortality. Parasitology 139(1):1\u201313. https:\/\/doi.org\/10.1017\/S0031182011001673","journal-title":"Parasitology"},{"issue":"5","key":"415_CR51","doi-asserted-by":"publisher","first-page":"2629","DOI":"10.4049\/jimmunol.164.5.2629","volume":"164","author":"H Kang","year":"2000","unstructured":"Kang H, Remington JS, Suzuki Y (2000) Decreased resistance of B cell-deficient mice to infection with Toxoplasma gondii despite unimpaired expression of IFN-gamma, TNF-alpha, and inducible nitric oxide synthase. J Immunol 164(5):2629\u20132634. https:\/\/doi.org\/10.4049\/jimmunol.164.5.2629","journal-title":"J Immunol"}],"container-title":["Acta Parasitologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11686-021-00415-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11686-021-00415-2\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11686-021-00415-2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,4]],"date-time":"2023-11-04T00:13:51Z","timestamp":1699056831000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11686-021-00415-2"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,21]]},"references-count":51,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["415"],"URL":"https:\/\/doi.org\/10.1007\/s11686-021-00415-2","relation":{},"ISSN":["1230-2821","1896-1851"],"issn-type":[{"value":"1230-2821","type":"print"},{"value":"1896-1851","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,21]]},"assertion":[{"value":"28 March 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 May 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 May 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors have no financial conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}}]}}