{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T15:53:35Z","timestamp":1786982015263,"version":"build-2736575974"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,8,21]],"date-time":"2021-08-21T00:00:00Z","timestamp":1629504000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,8,21]],"date-time":"2021-08-21T00:00:00Z","timestamp":1629504000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Parasit."],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      To perform environmental sampling and molecular identification of\n                      <jats:italic>Paragonimus<\/jats:italic>\n                      in endemic regions, which may help in minimizing transmission among humans.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Mountain crabs from the genus\n                      <jats:italic>Potamiscus<\/jats:italic>\n                      were collected and the encysted metacercariae were extracted and subjected to morphological identification, followed by animal inoculation in Sprague\u2013Dawley (SD) rats. After 112\u00a0days of infection, animals were killed and adult worms were extracted from lungs and muscles. The morphology of adult worms was characterized by microscopy and molecular identification was done by polymerase chain reaction, followed by sequencing of\n                      <jats:italic>cox1<\/jats:italic>\n                      and\n                      <jats:italic>ITS2<\/jats:italic>\n                      genes. Phylogenetic analysis was done by maximum parsimony method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 447 crabs were captured from the streams of Tongchang Town, Jinping County, Yunnan Province, China. The infection rate was found to be 41% (186 out of 447 crabs). The metacercariae of\n                      <jats:italic>Paragonimus skrjabini<\/jats:italic>\n                      was identified by the characteristics round or spherical encysted form measuring 410 to 460\u2009\u00d7\u2009400 to 460\u00a0\u00b5m. After animal infection in SD rats, adults were presumptively confirmed to be\n                      <jats:italic>P. skrjabini<\/jats:italic>\n                      , which was also confirmed by gene amplification and sequence analysis of\n                      <jats:italic>cox1<\/jats:italic>\n                      and\n                      <jats:italic>ITS2<\/jats:italic>\n                      regions.\n                      <jats:italic>Paragonimus skrjabini<\/jats:italic>\n                      clustered with previously reported\n                      <jats:italic>P. skrjabini<\/jats:italic>\n                      from Yunnan and Vietnam. The confidence values of their branches were\u2009&gt;\u200995%. Phylogenetic analysis of the\n                      <jats:italic>ITS2<\/jats:italic>\n                      region revealed two distinct clusters with distinct geographical grouping. Phylogenetic analysis with the combined data sets reiterated the geographical grouping with\n                      <jats:italic>P. skrjabini<\/jats:italic>\n                      from Yunnan clustering with strains from Vietnam.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Metacercariae of\n                      <jats:italic>P. skrjabini<\/jats:italic>\n                      was discovered in freshwater crabs in Yunnan province, China, and the strains were phylogenetically related to\n                      <jats:italic>P. skrjabini<\/jats:italic>\n                      from Vietnam.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11686-021-00461-w","type":"journal-article","created":{"date-parts":[[2021,8,20]],"date-time":"2021-08-20T22:02:37Z","timestamp":1629496957000},"page":"316-321","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Morphological and Molecular Characterization of Paragonimus skrjabini Complex from Yunnan, China: A Brief Report"],"prefix":"10.1007","volume":"67","author":[{"given":"Qiu-Hong","family":"Shu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-De","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Tian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Meng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu-Mei-Qi","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miao-Miao","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3754-4709","authenticated-orcid":false,"given":"Wen-Lin","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,8,21]]},"reference":[{"key":"461_CR1","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1093\/trstmh\/trv101","volume":"110","author":"D Blair","year":"2016","unstructured":"Blair D, Nawa Y, Mitreva M, Doanh PN (2016) Gene diversity and genetic variation in lung flukes (genus Paragonimus). 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