{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:41:23Z","timestamp":1790833283724,"version":"4.1.0"},"reference-count":26,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:00:00Z","timestamp":1637712000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:00:00Z","timestamp":1637712000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"funder":[{"DOI":"10.13039\/501100004214","name":"Pamukkale \u00dcniversitesi","doi-asserted-by":"publisher","award":["2017FBE067"],"award-info":[{"award-number":["2017FBE067"]}],"id":[{"id":"10.13039\/501100004214","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Parasit."],"published-print":{"date-parts":[[2022,3]]},"DOI":"10.1007\/s11686-021-00494-1","type":"journal-article","created":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T12:03:05Z","timestamp":1637755385000},"page":"555-559","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Comparative Evaluation of Re 529-Bp Sequence and B1 Gene in the Detection of Toxoplasma gondii Through PCR in Water Samples of Denizli, Turkey"],"prefix":"10.1007","volume":"67","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1654-2261","authenticated-orcid":false,"given":"Tu\u011fba","family":"Sa\u011flam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serdar","family":"D\u00fc\u015fen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ergun","family":"Mete","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00dclk\u00fc","family":"Karaman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2021,11,24]]},"reference":[{"key":"494_CR1","unstructured":"Anonymous (2009) Su kirlili\u011fi kontrol\u00fc y\u00f6netmeli\u011fi [Online]. Available: hptt:\/\/www.mevzuat.adalet.gov.tr\/html\/ 23053.html10. Accessed 15 December 2017"},{"key":"494_CR2","unstructured":"Miman \u00d6, Aktepe OC (2008) \u0130\u00e7me sular\u0131nda protozoon parazitlerin dezenfeksiyonu. Kocatepe T\u0131p Derg 9:31\u201335. https:\/\/dergipark.org.tr\/tr\/pub\/kocatepetip\/issue\/17415\/182373"},{"key":"494_CR3","unstructured":"Usluer G (2004) Su ile bula\u015fan enfeksiyonlar. Ankem Dergisi 18: 17\u201320. https:\/\/www.ankemdernegi.org.tr\/ANKEMJOURNALPDF\/ANKEM_18_Ek2_17_20.pdf"},{"issue":"8","key":"494_CR4","doi-asserted-by":"crossref","first-page":"2684","DOI":"10.4049\/jimmunol.143.8.2684","volume":"143","author":"BR Dannemann","year":"1989","unstructured":"Dannemann BR, Morris VA, Araujo FG, Remington J (1989) Assessment of human natural killer and Lymphokine-Activated killer cell cytotoxicity against Toxoplasma gondii trophozoites and brain cyst. J Immunol 143(8):2684\u20132691 (PMID: 2477453)","journal-title":"J Immunol"},{"issue":"3\u20134","key":"494_CR5","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/j.prevetmed.2012.01.003","volume":"104","author":"M Opsteegha","year":"2012","unstructured":"Opsteegha M, Havemana R, Swarta AN, Mensink-Beerepoota ME, Hofhuisa A, Langelaara MFM, van der Giessena JWB (2012) Seroprevalence and risk factors for Toxoplasma gondii infection in domestic cats in Tte Netherlands. Prev Vet Med 104(3\u20134):317\u2013326. https:\/\/doi.org\/10.1016\/j.prevetmed.2012.01.003","journal-title":"Prev Vet Med"},{"issue":"12\u201313","key":"494_CR6","doi-asserted-by":"publisher","first-page":"1217","DOI":"10.1016\/s0020-7519(00)00124-7","volume":"30","author":"AM Tenter","year":"2000","unstructured":"Tenter AM, Heckeroth AR, Weiss LM (2000) Toxoplasma gondii: from animals to humans. Int J Parasitol 30(12\u201313):1217\u20131258. https:\/\/doi.org\/10.1016\/s0020-7519(00)00124-7","journal-title":"Int J Parasitol"},{"issue":"1","key":"494_CR7","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1007\/s11356-012-1177-5","volume":"20","author":"P Karanis","year":"2013","unstructured":"Karanis P, Aldeyarbi HM, Mirhashemi ME, Khalil KM (2013) The impact of the waterborne transmission of Toxoplasma gondii and analysis efforts for water detection: an overview and update. Environ Sci Pollut Res 20(1):86\u201399. https:\/\/doi.org\/10.1007\/s11356-012-1177-5","journal-title":"Environ Sci Pollut Res"},{"issue":"15","key":"494_CR8","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1016\/j.watres.2015.09.015","volume":"87","author":"B Wells","year":"2015","unstructured":"Wells B, Shaw H, Innocent G, Guido S, Hotchkiss E, Parigi M, Opsteegh M, Green J, Gillespiee S, Innes EA, Katzera F (2015) Molecular detection of Toxoplasma gondii in water samples from Scotland and a comparison between the 529bp real-time PCR and ITS1 nested PCR. Water Res 87(15):175\u2013181. https:\/\/doi.org\/10.1016\/j.watres.2015.09.015","journal-title":"Water Res"},{"key":"494_CR9","doi-asserted-by":"publisher","first-page":"2278","DOI":"10.1128\/AEM.64.6.2278-2280.1998","volume":"64","author":"J Isaac-Renton","year":"1998","unstructured":"Isaac-Renton J, Bowie WR, King A, Irwin GS, Ong CS, Fung CP, Shokeir MO, Dubey JP (1998) Detection of Toxoplasma gondii oocysts in drinking water. Appl Environ Microbiol 64:2278\u20132280 (PMID: 9603850)","journal-title":"Appl Environ Microbiol"},{"issue":"1","key":"494_CR10","doi-asserted-by":"publisher","first-page":"287","DOI":"10.2166\/wst.2004.0069","volume":"50","author":"C Kourenti","year":"2004","unstructured":"Kourenti C, Karanis P (2004) Development of a sensitive polymerase chain reaction method for the detection of Toxoplasma gondii in water. Water Sci Technol 50(1):287\u2013291. https:\/\/doi.org\/10.2166\/wst.2004.0069","journal-title":"Water Sci Technol"},{"key":"494_CR11","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1111\/j.1472-765X.2006.02008.x","volume":"43","author":"C Kourenti","year":"2006","unstructured":"Kourenti C, Karanis P (2006) Evaluation and applicability of a purification method coupled with Nested PZR for the detection of Toxoplasma oocysts in water. Soc Appl Microbiol 43:475\u2013481. https:\/\/doi.org\/10.1111\/j.1472-765X.2006.02008.x","journal-title":"Soc Appl Microbiol"},{"issue":"11","key":"494_CR12","doi-asserted-by":"publisher","first-page":"3477","DOI":"10.1128\/AEM.00285-09","volume":"75","author":"W Yang","year":"2009","unstructured":"Yang W, Alan Lindquist HD, Cama V, Schaefer FW III, Villegas E, Fayer R, Lewis EJ, Feng Y, Xiao L (2009) Detection of Toxoplasma gondii oocysts in water sample concentrates by Real-Time PCR. ASM Appl Environ Microbiol 75(11):3477\u20133483. https:\/\/doi.org\/10.1128\/AEM.00285-09","journal-title":"ASM Appl Environ Microbiol"},{"key":"494_CR13","unstructured":"Fakir Y (2012) Denizli i\u00e7me suyu \u015febekesindeki su kalitesi parametrelerinin zamana ve konuma g\u00f6re de\u011fi\u015fiminin incelenmesi. Master's thesis, Institute of Science, University of Pamukkale. https:\/\/tez.yok.gov.tr\/UlusalTezMerkezi\/tezDetay.jsp?id=EoMXsCXN_dF7Af8Hbn7NEA&no=UFRGuc2KeFC81Ks56qnI7A"},{"key":"494_CR14","unstructured":"Deski (2015) Denizli b\u00fcy\u00fck\u015fehir belediyesi bu ve kanalizasyon idaresi (Denizli water and sewerage administration), Kentsel altyap\u0131 sistemleri sunumu (Urban infrastructure systems presentation). University of Pamukkale. https:\/\/www.deski.gov.tr\/"},{"key":"494_CR15","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1046\/j.1472-765x.2002.01121.x","volume":"34","author":"P Karanis","year":"2002","unstructured":"Karanis P, Kimura A (2002) Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from water samples. Lett Appl Microbiol 34:444\u2013449. https:\/\/doi.org\/10.1046\/j.1472-765x.2002.01121.x","journal-title":"Lett Appl Microbiol"},{"key":"494_CR16","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1128\/AEM.69.1.102-106.2003","volume":"69","author":"C Kourenti","year":"2003","unstructured":"Kourenti C, Heckeroth A, Tenter A, Karanis P (2003) Development and application of different methods for the detection of Toxoplasma gondii in water. Appl Environ Microbiol 69:102\u2013106. https:\/\/doi.org\/10.1128\/AEM.69.1.102-106.2003","journal-title":"Appl Environ Microbiol"},{"key":"494_CR17","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1016\/j.envres.2006.05.005","volume":"102","author":"P Karanis","year":"2006","unstructured":"Karanis P, Sotiriadou I, Kartashev V, Kourenti C, Tsvetkova N, Stojanova K (2006) Occurrence of Giardia and Cryptosporidium in water supplies of Russia and Bulgaria. Environ Res 102:260\u2013271. https:\/\/doi.org\/10.1016\/j.envres.2006.05.005","journal-title":"Environ Res"},{"key":"494_CR18","unstructured":"WHO (1991) Basic Laboratory methods in Medical Parasitology. Geneva WHO 1991. ISBN 9241544104"},{"key":"494_CR19","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.parint.2013.08.005","volume":"63","author":"SH Fallahi","year":"2014","unstructured":"Fallahi SH, Kazemi B, Seyyed Tabaei SJ, Bandehpour M, Lasjerdi Z, Taghipour N, Zebardst N, Nikmanesh B, Fallah Omrani V, Ebrahimzadeh F (2014) Comparasion of the RE and B1 gene for detection of Toxoplasma gondii infection in children with cancer. Parasitol Int 63:37\u201341. https:\/\/doi.org\/10.1016\/j.parint.2013.08.005","journal-title":"Parasitol Int"},{"key":"494_CR20","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/s0020-7519(99)00170-8","volume":"30","author":"WL Homan","year":"2000","unstructured":"Homan WL, Vercammen M, De Braekeleer J, Verschueren H (2000) Identification of a 200-to 300-fold repetitive 529 bp DNA fragment in Toxoplasma gondii, and its for diagnostic an quantiative PCR. Int J Parasitol 30:69\u201375. https:\/\/doi.org\/10.1016\/s0020-7519(99)00170-8","journal-title":"Int J Parasitol"},{"issue":"2","key":"494_CR21","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1111\/j.1469-0691.2005.01332.x","volume":"12","author":"B Edvinsson","year":"2006","unstructured":"Edvinsson B, Lappalainen M, Eveng\u00e5rd B (2006) Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosis. Clin Microbiol Infect 12(2):131\u2013136. https:\/\/doi.org\/10.1111\/j.1469-0691.2005.01332.x","journal-title":"Clin Microbiol Infect"},{"key":"494_CR22","doi-asserted-by":"publisher","first-page":"619","DOI":"10.1007\/s00436-007-0524-9","volume":"101","author":"A Kompalic-Cristo","year":"2007","unstructured":"Kompalic-Cristo A, Frotta C, Su\u00e1rez-Mutis M, Fernandes O, Britto C (2007) Evaluation of a real-time PCR assay based on the repetitive B1 gene for the detection of Toxoplasma gondii in human peripheral blood. Parasitol Res 101:619\u2013625. https:\/\/doi.org\/10.1007\/s00436-007-0524-9","journal-title":"Parasitol Res"},{"key":"494_CR23","first-page":"634","volume":"41","author":"CD Jones","year":"2000","unstructured":"Jones CD, Okhravi N, Adamson P, Tasker S, Lightman S (2000) Comparison of PCR detection methods for B1, P30, and 18S rDNA genes of T. gondii in aqueous humor. Invest Ophthalmol Vis Sci 41:634\u2013644","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"494_CR24","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.vetpar.2006.05.008","volume":"141","author":"DE Hill","year":"2006","unstructured":"Hill DE, Chirukandoth S, Dubey JP, Lunney JK, Gamble HR (2006) Comparison of detection methods for Toxoplasma gondii in naturally and experimentally infected swine. Vet Parasitol 141:9\u201317. https:\/\/doi.org\/10.1016\/j.vetpar.2006.05.008","journal-title":"Vet Parasitol"},{"key":"494_CR25","doi-asserted-by":"crossref","unstructured":"Demirel E, Kol\u00f6ren Z, Karaman \u00dc, Ayaz E (2014) Giresun\u2019da su \u00f6rneklerinde Toxoplasma gondii\u2019nin polimeraz zincir reaksiyonu ve ilmi\u011fe dayal\u0131 izotermal amplifikasyon y\u00f6ntemleriyle ara\u015ft\u0131r\u0131lmas\u0131. Mikrobiyol Bul 48(4): 661\u2013668. ISSN:\u00a00374-9096","DOI":"10.5578\/mb.8098"},{"key":"494_CR26","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2021.109515","volume":"296","author":"A Fl\u00e1via Minutti","year":"2021","unstructured":"Fl\u00e1via Minutti A, Gon\u00e7alves Vieira FA, Pedro Sasse J, Agostinho Martins T, de Seixas M, Tosi Cardim S, de Barros LD, Luis Garcia J (2021) Comparison of serological and molecular techniques to detect Toxoplasma gondii in free-range chickens (Gallus gallus domesticus). Vet Parasitol 296:109515. https:\/\/doi.org\/10.1016\/j.vetpar.2021.109515","journal-title":"Vet Parasitol"}],"container-title":["Acta Parasitologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11686-021-00494-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11686-021-00494-1\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11686-021-00494-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T23:16:15Z","timestamp":1744154175000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11686-021-00494-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,24]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["494"],"URL":"https:\/\/doi.org\/10.1007\/s11686-021-00494-1","relation":{},"ISSN":["1230-2821","1896-1851"],"issn-type":[{"value":"1230-2821","type":"print"},{"value":"1896-1851","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,11,24]]},"assertion":[{"value":"11 September 2021","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"4 November 2021","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 November 2021","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"The authors declare that they have no competing interests.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of Interest"}},{"value":"Ethics committee approval was not received due to working in water.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}},{"value":"Not applicable.","order":4,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent to Participate"}},{"value":"Not applicable.","order":5,"name":"Ethics","group":{"name":"EthicsHeading","label":"Consent for Publication"}}]}}