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The disease is caused by the biting fly-transmitted subspecies <jats:italic>Trypanosoma brucei evansi<\/jats:italic>, which is very closely related to the tsetse-transmitted subspecies <jats:italic>T. b. brucei<\/jats:italic> and the sexually transmitted subspecies <jats:italic>T. b. equiperdum<\/jats:italic>. At least two phylogenetically distinct groups of <jats:italic>T. b. evansi<\/jats:italic> can be distinguished, called type A and type B. These evolved from <jats:italic>T. b. brucei<\/jats:italic> independently. The close relationships between the <jats:italic>T. brucei<\/jats:italic> subspecies and the multiple evolutionary origins of <jats:italic>T. b. evansi<\/jats:italic> pose diagnostic challenges.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Methods<\/jats:title>\n                <jats:p>Here we use previously established and newly developed PCR assays based on nuclear and mitochondrial genetic markers to type the causative agent of recent trypanosome infections of camels in Southern Algeria.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results\/conclusion<\/jats:title>\n                <jats:p>We confirm that these infections have been caused by <jats:italic>T. b. evansi<\/jats:italic> type A. We also report a newly designed PCR assay specific for <jats:italic>T. b. evansi<\/jats:italic> type A that we expect will be of diagnostic use for the community.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s11686-022-00577-7","type":"journal-article","created":{"date-parts":[[2022,6,3]],"date-time":"2022-06-03T13:04:13Z","timestamp":1654261453000},"page":"1246-1253","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular Analysis of Trypanosome Infections in Algerian Camels"],"prefix":"10.1007","volume":"67","author":[{"given":"Djamila","family":"Boushaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Wallis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederik","family":"Van den Broeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2132-5560","authenticated-orcid":false,"given":"Achim","family":"Schnaufer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,6,3]]},"reference":[{"key":"577_CR1","doi-asserted-by":"publisher","first-page":"e3404","DOI":"10.1371\/journal.pntd.0003404","volume":"9","author":"J Carnes","year":"2015","unstructured":"Carnes J, Anupama A, Balmer O, Jackson A, Lewis M, Brown R et al (2015) Genome and phylogenetic analyses of Trypanosoma evansi reveal extensive similarity to T. brucei and multiple independent origins for dyskinetoplasty. 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At each wilaya, the study had been authorized and supervised by the respective Inspection V\u00e9t\u00e9rinaire de Wilaya (IVW B\u00e9char, El Bayadh, Ouargla, and Tamanrasset), operating under the umbrella of the Direction des Services V\u00e9t\u00e9rinaires. All experiments in Achim Schnaufer\u2019s laboratory are carried out after local ethical approval at the University of Edinburgh by the School of Biological Sciences Ethics Committee (application \u201caschnauf-0002 Mitochondrial biology of trypanosomes\u201d).","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}}]}}