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K\u00fc\u00e7\u00fck ve Orta \u00d6l\u00e7ekli \u0130\u015fletmeleri Geli\u015ftirme ve Destekleme \u0130daresi Ba\u015fkanl\u0131\u011f\u0131","doi-asserted-by":"publisher","award":["2020-682-3"],"award-info":[{"award-number":["2020-682-3"]}],"id":[{"id":"10.13039\/100020507","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003010","name":"Ege University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003010","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Parasit."],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Background<\/jats:title>\n                <jats:p>Cryptosporidiosis is a disease that causes major intestinal damage in humans and animals. The causative agents of the disease are <jats:italic>Cryptosporidium<\/jats:italic> species. In newborn calves, diarrhea can lead to death, resulting in significant economic losses for the farms. Therefore, accurate, rapid, and cost-effective diagnosis of the disease is very important.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Material and methods<\/jats:title>\n                <jats:p>In this study, a novel colorimetric loop-mediated isothermal amplification (LAMP) test named \u201cRapid-Crypto Colorimetric LAMP test\u201d targeting <jats:italic>Cryptosporidium<\/jats:italic> spp. <jats:italic>18S rRNA<\/jats:italic> gene was developed to detect cryptosporidiosis in the feces of newborn calves. The analytical sensitivity of the test was determined by plasmid controls. Clinical sensitivity was determined using the feces of 127 calves collected from farms in \u0130zmir and Manisa provinces. All of the samples were also investigated with Real-Time PCR targeting the <jats:italic>Cryptosporidium<\/jats:italic> spp. <jats:italic>COWP<\/jats:italic> gene. Cross-reactivity was tested using the DNA of other parasites and bacteria.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>According to the results, the analytical sensitivity of the \u201cRapid-Crypto Colorimetric LAMP test\u201d was found as 1 copy plasmid\/reaction. When the results were compared with the Real-Time PCR test, the sensitivity of the \u201cRapid-Crypto Colorimetric LAMP test\u201d was 100% and the specificity was 97.4%. The test did not cross-react with other parasites and bacteria.  \u00a0<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>The \u201cRapid-Crypto Colorimetric LAMP test\u201d developed in this study provides an advantage in the diagnosis of <jats:italic>Cryptosporidium<\/jats:italic> spp. in calf stool samples since it can be applied in basic laboratories or in the field, does not require experienced personnel, and has high sensitivity. Moreover, diagnosis can be made with the naked eye without using any device.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s11686-023-00791-x","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T07:02:39Z","timestamp":1707980559000},"page":"691-699","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Development of a \u201cRapid-Crypto Colorimetric LAMP Test\u201d to Detect Cryptosporidiosis in Feces of Newborns Calves"],"prefix":"10.1007","volume":"69","author":[{"given":"Muhammet","family":"Karakavuk","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00fcseyin","family":"Can","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u015eeng\u00fcl","family":"Can","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tu\u011fba","family":"Karakavuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mert","family":"D\u00f6\u015fkaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0363-9099","authenticated-orcid":false,"given":"Aysu","family":"De\u011firmenci D\u00f6\u015fkaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2024,2,15]]},"reference":[{"key":"791_CR1","doi-asserted-by":"publisher","first-page":"5660","DOI":"10.1128\/AEM.01152-07","volume":"73","author":"P Karanis","year":"2007","unstructured":"Karanis P, Thekisoe O, Kiouptsi K, Ongerth J, Igarashi I, Inoue N (2007) Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples. 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All experimental studies were carried out in accordance with ARRIVE guidelines.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethical Approval"}}]}}