{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T06:47:32Z","timestamp":1785653252036,"version":"3.56.0"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T00:00:00Z","timestamp":1744243200000},"content-version":"vor","delay-in-days":9,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100003010","name":"Ege University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003010","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Parasit."],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Purpose<\/jats:title>\n            <jats:p>Anaplasmosis is a tick-borne disease which is caused by different <jats:italic>Anaplasma<\/jats:italic> species. Among <jats:italic>Anaplasma<\/jats:italic> species, <jats:italic>A. ovis<\/jats:italic> which can infect sheep and goats cause ovine anaplasmosis. In this study, the prevalence of <jats:italic>Anaplasma<\/jats:italic> spp. was investigated in 31 different breeds of sheep from different regions of T\u00fcrkiye.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Method<\/jats:title>\n            <jats:p>\n              <jats:italic>Anaplasma<\/jats:italic> spp. was investigated by PCR targeting <jats:italic>MSP-4<\/jats:italic> gene in blood samples of sheep breeds (n\u00a0=\u00a0366) collected from different regions of T\u00fcrkiye. Also, some <jats:italic>Anaplasma<\/jats:italic> spp. positive samples were sequenced for species identification and sub-cluster analyses.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>The molecular prevalence of <jats:italic>Anaplasma<\/jats:italic> spp. was 43.9% (161\/366). In Anatolian Merino (n\u00a0=\u00a010) and Akkaraman (n\u00a0=\u00a011) breeds, the molecular prevalence of <jats:italic>Anaplasma<\/jats:italic> spp. reached to 100%. Also, the highest molecular prevalence was detected in Black Sea region by 70% (28\/40) and the lowest molecular prevalence was detected in Marmara region by 32% (16\/50). While the prevalence of <jats:italic>Anaplasma<\/jats:italic> spp. was 59.7% in sheep produced in the extensive system, it was found as 39.2% and 9.8% in sheep produced in semi-extensive and intensive systems, respectively. Accordingly, these findings suggest that the production of sheep in the intensive system protects them from tick-borne diseases, which are of great economic importance. According to BLAST results, all sequenced <jats:italic>Anaplasma<\/jats:italic> spp. positive samples (n\u00a0=\u00a029) were identified as <jats:italic>A. ovis<\/jats:italic>. Also, mixed infections were detected in 6 positive samples. The phylogenetic tree constructed by 38 sequence data showed the presence of three different sub-clusters for <jats:italic>A. ovis<\/jats:italic> (Sub-cluster 1, 2, and 3). Sub-cluster 2 was found as the most prevalent sub-cluster with 42.1% frequency compared to the other sub-clusters.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>This study showed that sheep grown in different regions of T\u00fcrkiye have a high molecular prevalence value for <jats:italic>Anaplasma<\/jats:italic> spp.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s11686-025-01021-2","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T16:47:31Z","timestamp":1744303651000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Molecular Investigation of Anaplasma spp. and Genotype Profile of A. ovis in Sheep from Different Farms in T\u00fcrkiye"],"prefix":"10.1007","volume":"70","author":[{"given":"\u00d6zge","family":"D\u00fclek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c7a\u011fr\u0131","family":"Kandemir","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ecem Su","family":"Ko\u00e7kaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ecem","family":"S\u00fcrge\u00e7","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mervenur","family":"G\u00fcvendi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammet","family":"Karakavuk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aysu","family":"De\u011firmenci D\u00f6\u015fkaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00d6zlem","family":"G\u00fcnay-Esiyok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Turgay","family":"Ta\u015fk\u0131n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mert","family":"D\u00f6\u015fkaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cemal","family":"\u00dcn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adnan Y\u00fcksel","family":"G\u00fcr\u00fcz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmet Efe","family":"K\u00f6seo\u011flu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sedef","family":"Erkunt Alak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erkan","family":"Pehlivan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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