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Moreover, identifying drugs can effectively eliminate and successfully treat the infection with\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Sixty male Swiss albino mice were divided into six groups, each of 10 mice. G1 served as the control, and G2 was inoculated with 10\n                      <jats:sup>4<\/jats:sup>\n                      oocysts. Mice were orally administered NZ (200\u00a0mg\/kg b.wt.), LF (150\u00a0\u00b5g\/kg b.wt.), or LF-NC (150\u00a0mg\/kg b.wt.\/day) for a period of six days post-infection. The immunomodulatory potential and drug efficacy were assessed by fecal and intestinal oocyst counts, measuring antioxidant activities and cytokine levels in addition. Intestinal tissues were examined histopathologically.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The cytokines TGF-\u03b2, INF-\u03b3, and IL-10 were increased during infection and decreased following therapy. Mutually, the quantity of pathological lesions and oocysts in the ileal tissues was significantly diminished with the NZ plus LF-NC treatment. In addition, a considerable reduction of both fecal and intestinal samples, as well as a notable deterioration in oocyst counts, suggested that treatment with NZ alone or in combination with LF-NC was more effective.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>LF or LF-NC was found to exert a potent immunomodulatory effect on infected mice as well as minimize pathological lesions. This therapy approaches a successful therapeutic alternative for the treatment of cryptosporidiosis with few adverse effects.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11686-025-01022-1","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T05:19:04Z","timestamp":1747372744000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Oral Treatment of Lactoferrin Nanocapsules Modulates the Immune Response of Mice to a Cryptosporidiosis Infection"],"prefix":"10.1007","volume":"70","author":[{"given":"Ibrahim","family":"Aly","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Waleed E.","family":"Elawamy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanan T.","family":"Hamza","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hany M.","family":"El-Wahsh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed","family":"EL-Bahiry","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amira","family":"Matter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lamia I.","family":"Bakr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,5,16]]},"reference":[{"issue":"8","key":"1022_CR1","doi-asserted-by":"publisher","first-page":"e593","DOI":"10.1016\/j.ijid.2012.11.023","volume":"17","author":"AG Abdou","year":"2013","unstructured":"Abdou AG, Harba NM, Afifi AF, Elnaidany NF (2013) Assessment of Cryptosporidium parvum infection in immunocompetent and immunocompromised mice and its role in triggering intestinal dysplasia. 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