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Among all participants, 86 were asymptomatic, while 64 experienced symptoms. PCR analysis confirmed <jats:italic>Blastocystis<\/jats:italic> in 26 out of the 42 cases. PCR-restriction fragment length polymorphism (RFLP) analysis identified 60% of isolates as Group A and 16% as Group C, while sequencing later confirmed that 24% belonged to Group B. PCR-sequence-tagged site (STS) analysis revealed five STs (ST1, ST2, ST3, ST5, and ST7), with ST1 (52%) and ST3 (24%) identified as the most prevalent STs.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>\n              <jats:italic>Blastocystis<\/jats:italic> infection was significantly higher in CRC patients, suggesting a possible association with the disease. It appears to act as an opportunistic pathogen, contributing to symptom development regardless of CRC. The absence of significant differences in ST distribution across groups indicates that <jats:italic>Blastocystis<\/jats:italic> pathogenicity is complex and not exclusively linked to specific STs.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s11686-025-01079-y","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T13:56:02Z","timestamp":1752501362000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Insights into the Association Between Blastocystis Infection and Colorectal Cancer"],"prefix":"10.1007","volume":"70","author":[{"given":"Amel","family":"Shehab","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona","family":"El-Sayad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal","family":"Allam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bassam","family":"Mohamed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasha","family":"Elsaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa","family":"Ibrahim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naglaa Abd","family":"El-Latif","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"key":"1079_CR1","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1016\/j.rvsc.2020.09.031","volume":"135","author":"JSY Hublin","year":"2021","unstructured":"Hublin JSY, Maloney JG, Santin M (2021) Blastocystis in domesticated and wild mammals and birds. 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