{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T03:39:13Z","timestamp":1789011553543,"version":"build-2803163510"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100011644","name":"Kahramanmaras Sutcu Imam University","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100011644","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Parasit."],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Purpose<\/jats:title>\n            <jats:p>Anti-tumor immune responses and certain pathogens can exhibit various effects, including signals that reduce the risk of tumor formation or lead to cancer regression. Multiple studies have reported that infectious agents and the products of a wide range of host structures can modulate cancer development and growth positively or negatively and regulate the activation of immune responses. Numerous studies have reported that the parasite <jats:italic>Echinococcus granulosus<\/jats:italic> may have anti-cancer or carcinogenic effects on cancer cell proliferation in various cell cultures and animal models. The primary purpose of the study is to investigate the effect of animal-derived hydatid cyst fluid (HCF) at various concentrations (1\/2, 1\/3, 1\/5) on the viability of human colorectal adenocarcinoma (Caco-2) and human colon epithelial (CoEpi) cell lines using the cell proliferation assay (XTT).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Subsequently, the study aims to investigate cytokine concentrations and gene expression profiles of inflammatory cytokines TNF-\u03b1 and IL-4, as well as epithelial-mesenchymal transition (EMT) regulatory signaling proteins TGF- \u03b21, vimentin and E-cadherin, using ELISA and RT-PCR methods, respectively.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Following the HCF application, EMT was consistently detected in the Caco-2 cell line compared to the CoEpi cell line in the 1\/5 volume application group, as confirmed by ELISA, RT-PCR and cell proliferation assays. On the other hand, a linear relationship was observed between the levels of TGF-\u03b21 and TNF-\u03b1, which regulate the pro-inflammatory signaling mechanism based on the cell micro-environment and the decrease in cell viability. As the HCF concentration volume decreased (1\/5), an increase in cell viability was observed (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), along with an increase in TGF-\u03b21 and TNF-\u03b1 levels. Otherwise, in Caco-2 cells, as the HCF application concentrations increased (1\/2), significant decreases in TGF-\u03b21 and TNF-\u03b1 levels, as well as in cell viability, were observed (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01).<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusions<\/jats:title>\n            <jats:p>In this context, the common antigen-receptor structure between <jats:italic>E. granulosus<\/jats:italic> and cancer may modulate the signals of the immune response it regulates, affecting immune system cells and contributing to the progression of tumor cells.<\/jats:p>\n          <\/jats:sec>","DOI":"10.1007\/s11686-025-01086-z","type":"journal-article","created":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T09:40:06Z","timestamp":1751881206000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Effects of Hydatid Cyst Fluid on Inflammation and Epithelial-Mesenchymal Transition in Colorectal Adenocarcinoma (Caco-2) Cell Line"],"prefix":"10.1007","volume":"70","author":[{"given":"Emin","family":"Yagmur","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u0130pek","family":"Baysal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serra","family":"\u00d6rsten","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma \u0130nan\u00e7","family":"Tolun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,7,7]]},"reference":[{"issue":"7","key":"1086_CR1","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1016\/S1473-3099(18)30221-4","volume":"18","author":"F -Tamarozzi","year":"2018","unstructured":"-Tamarozzi F, Akhan O, Cretu CM, Vutova K, Akinci D, Chipeva R et al (2018) Prevalence of abdominal cystic echinococcosis in rural bulgaria, romania, and turkey: a cross-sectional, ultrasound-based, population study from the HERACLES project. Lancet Infect Dis 18(7):769\u2013778","journal-title":"Lancet Infect Dis"},{"key":"1086_CR2","doi-asserted-by":"crossref","unstructured":"-Eckert J, Deplazes P (2004) Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. 17(1):107\u2013135","DOI":"10.1128\/CMR.17.1.107-135.2004"},{"issue":"3","key":"1086_CR3","first-page":"518","volume":"54","author":"E -Brunetti","year":"2016","unstructured":"-Brunetti E, Mccloskey C (2016) Cyst Echinococcosis 54(3):518\u2013523","journal-title":"Cyst Echinococcosis"},{"issue":"1","key":"1086_CR4","doi-asserted-by":"publisher","first-page":"9","DOI":"10.4103\/2277-9175.175242","volume":"5","author":"S -Daneshpour","year":"2016","unstructured":"-Daneshpour S, Bahadoran M, Hejazi S, Eskandarian A, Mahmoudzadeh M, Darani H (2016) Common antigens between hydatid cyst and cancers. Adv Biomed Res 5(1):9","journal-title":"Adv Biomed Res"},{"issue":"1","key":"1086_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.54962\/ankemderg.1107804","volume":"36","author":"\u0130 -Baysal","year":"2022","unstructured":"-Baysal \u0130, \u00d6rsten S (2022) Echinococcus granulosus sensu stricto Kaynakl\u0131 h\u0131\u0307dat\u0131\u0307k k\u0131\u0307st S\u0131v\u0131s\u0131 Uygulamas\u0131n\u0131n Caco-2 H\u00fccre Hattinda Ep\u0131\u0307telyal-Mezenk\u0131\u0307mal ge\u00e7\u0131\u0307\u015f ve Apoptoz \u00fczer\u0131\u0307n etk\u0131\u0307s\u0131\u0307. Ankem Derg 36(1):1\u20138","journal-title":"Ankem Derg"},{"issue":"17","key":"1086_CR6","doi-asserted-by":"publisher","first-page":"4669","DOI":"10.1158\/1078-0432.CCR-20-4459","volume":"27","author":"M -Taki","year":"2021","unstructured":"-Taki M, Abiko K, Ukita M, Murakami R, Yamanoi K, Yamaguchi K et al (2021) Tumor immune microenvironment during epithelial- mesenchymal transition. Clin Cancer Res 27(17):4669\u20134679","journal-title":"Clin Cancer Res"},{"key":"1086_CR7","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1146\/annurev-pathol-020117-043854","volume":"13","author":"V -Mittal","year":"2018","unstructured":"-Mittal V (2018) Epithelial mesenchymal transition in tumor metastasis. Annu Rev Pathol Mech Dis 13:395\u2013412","journal-title":"Annu Rev Pathol Mech Dis"},{"key":"1086_CR8","doi-asserted-by":"publisher","first-page":"230176","DOI":"10.1155\/2013\/230176","volume":"2013","author":"E -Berriel","year":"2013","unstructured":"-Berriel E, Russo S, Monin L, Festari MF, Berois N, Fern\u00e1ndez G, Freire T, Osinaga E (2013) Antitumor activity of human hydatid cyst fluid in a murine model of colon cancer. ScientificWorldJournal 2013:230176. https:\/\/doi.org\/10.1155\/2013\/230176PMID: 24023528; PMCID: PMC3759250","journal-title":"ScientificWorldJournal"},{"key":"1086_CR9","doi-asserted-by":"publisher","unstructured":"-Rostamirad S, Daneshpour S, Mofid MR, Andalib A, Eskandariyan A, Mousavi S, Yousofi Darani H (2023) Inhibition of mouse colon cancer growth following immunotherapy with a fraction of hydatid cyst fluid. Exp Parasitol.;249:108501. https:\/\/doi.org\/10.1016\/j.exppara.2023.108501. Epub 2023 Mar 15. PMID: 36931383","DOI":"10.1016\/j.exppara.2023.108501"},{"key":"1086_CR10","doi-asserted-by":"publisher","first-page":"108829","DOI":"10.1016\/j.exppara.2024.108829","volume":"265","author":"H -Mosajakhah","year":"2024","unstructured":"-Mosajakhah H, Shanehbandi D, Ahmadpour E, Mahami-Oskouei M, Sadeghi K, Spotin A (2024) MicroRNA-145 enhances lung cancer cell progression after exposure to lyophilized fertile hydatid cyst fluid of Echinococcus granulosus sensu stricto. Exp Parasitol 265:108829. https:\/\/doi.org\/10.1016\/j.exppara.2024.108829Epub 2024 Aug 22. PMID: 39179144","journal-title":"Exp Parasitol"},{"key":"1086_CR11","first-page":"3","volume":"2","author":"N -Aref","year":"2012","unstructured":"-Aref N, Shirzad H, Yousefi M, Darani H (2012) Effect of different hydatid cyst molecules on Hela and Vero cell lines growth in vitro. J Immunodefic Disor 2:3","journal-title":"J Immunodefic Disor"},{"issue":"9","key":"1086_CR12","doi-asserted-by":"publisher","first-page":"1999","DOI":"10.1002\/cncr.11752","volume":"98","author":"H -Akg\u00fcl","year":"2003","unstructured":"-Akg\u00fcl H, Tez M, Unal AE, Ke\u015fkek M, Sayek I, Oz\u00e7elik T (2003) Echinococcus against cancer: why not? Cancer 98(9):1999\u20132000","journal-title":"Cancer"},{"issue":"9","key":"1086_CR13","doi-asserted-by":"publisher","first-page":"1995","DOI":"10.1002\/cncr.11751","volume":"98","author":"CFM -Sier","year":"2003","unstructured":"-Sier CFM, Blasi F, Sidenius N, Shariat SF, Lerner SP, Casella R (2003) Urinary levels of 69 Urokinase-Type plasminogen activator and its receptor in the detection of bladder carcinoma [1] (multiple letters). Cancer 98(9):1995","journal-title":"Cancer"},{"key":"1086_CR14","doi-asserted-by":"crossref","unstructured":"-Yousofi Darani H, Soozangar N, Khorami S, Taji F, Yousofi M, Shirzad H (2012) Hydatid cyst protoscolices induce cell death in WEHI-164 fibrosarcoma cells and inhibit the proliferation of baby hamster kidney fibroblasts in vitro. J Parasitol Res.;2012","DOI":"10.1155\/2012\/304183"},{"issue":"2","key":"1086_CR15","doi-asserted-by":"crossref","first-page":"248","DOI":"10.5578\/mb.20219910","volume":"55","author":"I -Baysal","year":"2021","unstructured":"-Baysal I, \u00d6rsten S (2021) Evaluation of the effect of hydatid cyst fluid on the apoptosis pathway in BEAS-2B and A549 cell lines. Mikrobiyol Bul 55(2):248\u2013255","journal-title":"Mikrobiyol Bul"},{"key":"1086_CR16","doi-asserted-by":"crossref","unstructured":"-Zhao L, Li C, Zhou B, Luo C, Wang Y, Che L et al (2019) Crucial role of serum response factor in renal tubular epithelial cell epithelial-mesenchymal transition in hyperuricemic nephropathy. 11(22):10597\u201310609","DOI":"10.18632\/aging.102479"},{"key":"1086_CR17","doi-asserted-by":"crossref","unstructured":"-Zhong X, Tang J, Li H, Shi X, Wu Y, Xia D (2020) MiR-3175 promotes epithelialmesenchymal transition by targeting Smad7 in human conjunctiva and pterygium. 594:1207\u20131217","DOI":"10.1002\/1873-3468.13698"},{"key":"1086_CR18","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fphar.2019.01137","volume":"10","author":"W -Guan","year":"2019","unstructured":"-Guan W, Zhang X, Wang X, Lu S, Yin J, Zhang J (2019) Employing parasite against cancer: A lesson from the canine tapeworm Echinococcus granulocus. Front Pharmacol 10:1\u20138","journal-title":"Front Pharmacol"},{"issue":"2","key":"1086_CR19","first-page":"2985","volume":"8","author":"H -Li","year":"2015","unstructured":"-Li H, Song T, Shao Y, Wen H (2015) Cystic echinococcosis accompanied by hepatocellular carcinoma in a female herdsman. Int J Clin Exp Med 8(2):2985\u20132988","journal-title":"Int J Clin Exp Med"},{"issue":"11","key":"1086_CR20","doi-asserted-by":"publisher","first-page":"2834","DOI":"10.1158\/1078-0432.CCR-12-3661","volume":"19","author":"K -Oikonomopoulou","year":"2013","unstructured":"-Oikonomopoulou K, Brinc D, Kyriacou K, Diamandis EP (2013) Infection and cancer: Revaluation of the hygiene hypothesis. Clin Cancer Res 19(11):2834\u20132841","journal-title":"Clin Cancer Res"},{"issue":"4","key":"1086_CR21","first-page":"647","volume":"26","author":"J -Noguti","year":"2012","unstructured":"-Noguti J, Barbisan LF, Cesar A, Dias Seabra C, Choueri RB, Ribeiro DA (2012) Review: in vivo models for measuring placental glutatione-S-transferase (GST-P 7\u20137) levels: a suitable biomarker for Understanding cancer pathogenesis. Vivo 26(4):647\u2013650","journal-title":"Vivo"},{"issue":"1","key":"1086_CR22","first-page":"10","volume":"39","author":"S -Turk","year":"2019","unstructured":"-Turk S, Baysal I, Ucar G (2019) Glutathione S-transferase-\u03c0 levels of in vitro generated epithelial mesenchymal transition model in caco-2 cell line. Hacettepe Univ J Fac Pharm 39(1):10\u201316","journal-title":"Hacettepe Univ J Fac Pharm"},{"key":"1086_CR23","doi-asserted-by":"publisher","first-page":"15121520","DOI":"10.1111\/j.1349-7006.2007.00550.x","volume":"98","author":"A -Moustakas","year":"2007","unstructured":"-Moustakas A, Heldin C-H (2007) Signaling networks guiding epithelial-mesenchymal transitions during embryogenesis and cancer progression. Cancer Sci 98:15121520","journal-title":"Cancer Sci"},{"issue":"8","key":"1086_CR24","doi-asserted-by":"publisher","first-page":"314","DOI":"10.2183\/pjab.85.314","volume":"85","author":"K -Miyazono","year":"2009","unstructured":"-Miyazono K (2009) Transforming growth factor-\u03b2 signaling in epithelial-mesenchymal transition and progression of cancer. Proc Japan Acad Ser B Phys Biol Sci 85(8):314\u2013323","journal-title":"Proc Japan Acad Ser B Phys Biol Sci"},{"issue":"1","key":"1086_CR25","first-page":"49","volume":"21","author":"B -Bierie","year":"2011","unstructured":"-Bierie B, Moses HL (2011) Cancer Cytokine 21(1):49\u201359","journal-title":"Cancer Cytokine"},{"key":"1086_CR26","doi-asserted-by":"publisher","first-page":"1","DOI":"10.3389\/fimmu.2021.810286","volume":"12","author":"ZW -Liu","year":"2022","unstructured":"-Liu ZW, Zhang YM, Zhang LY, Zhou T, Li YY, Zhou GC et al (2022) Duality of interactions between TGF-\u03b2 and TNF-\u03b1 during tumor formation. Front Immunol 12:1\u201314","journal-title":"Front Immunol"},{"issue":"7","key":"1086_CR27","doi-asserted-by":"publisher","first-page":"371","DOI":"10.1007\/s11626-009-9190-9","volume":"45","author":"R -Paduch","year":"2009","unstructured":"-Paduch R, Niedziela P (2009) TGF-beta1 influence on TNF-alpha production and sTNF-Rs shedding in a coculture of colon carcinoma cell spheroids with normal cells. Vitro Cell Dev Biol Anim 45(7):371\u2013377","journal-title":"Vitro Cell Dev Biol Anim"},{"issue":"11","key":"1086_CR28","doi-asserted-by":"publisher","first-page":"1717","DOI":"10.1084\/jem.20031701","volume":"198","author":"K -Suzuki","year":"2003","unstructured":"-Suzuki K, Nakajima H, Ikeda K, Maezawa Y, Suto A, Takatori H, Saito Y, Iwamoto I (2003) IL-4-Stat6 signaling induces tristetraprolin expression and inhibits TNF-alpha production in mast cells. J Exp Med 198(11):1717\u20131727. https:\/\/doi.org\/10.1084\/jem.20031701Epub 2003 Nov 24. PMID: 14638848; PMCID: PMC2194141","journal-title":"J Exp Med"},{"issue":"4","key":"1086_CR29","doi-asserted-by":"publisher","first-page":"978","DOI":"10.1158\/1055-9965.EPI-10-0064","volume":"19","author":"FM -Shebl","year":"2010","unstructured":"-Shebl FM, Pinto LA, Garc\u00eda-Pi\u00f1eres A, Lempicki R, Williams M, Harro C, Hildesheim A (2010) Comparison of mRNA and protein measures of cytokines following vaccination with human papillomavirus-16 L1 virus-like particles. Cancer Epidemiol Biomarkers Prev 19(4):978\u2013981. https:\/\/doi.org\/10.1158\/1055-9965.EPI-10-0064Epub 2010 Mar 23. PMID: 20332253; PMCID: PMC2852493","journal-title":"Cancer Epidemiol Biomarkers Prev"}],"container-title":["Acta Parasitologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11686-025-01086-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11686-025-01086-z\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11686-025-01086-z.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T06:31:07Z","timestamp":1757485867000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11686-025-01086-z"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,7]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,8]]}},"alternative-id":["1086"],"URL":"https:\/\/doi.org\/10.1007\/s11686-025-01086-z","relation":{},"ISSN":["1230-2821","1896-1851"],"issn-type":[{"value":"1230-2821","type":"print"},{"value":"1896-1851","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,7]]},"assertion":[{"value":"30 April 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 June 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 July 2025","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"Ethics committee approval is not required for this study, as cell culture was performed using commercially obtained cell lines and hydatid cyst fluid was obtained from a slaughterhouse.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Committee Approval"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing Interests"}}],"article-number":"146"}}