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This study aimed to assess the therapeutic efficacy of\n                      <jats:italic>Allium sativum<\/jats:italic>\n                      (garlic) extract loaded onto Fe-MOFs in a murine model of chronic toxoplasmosis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Sixty-five mice were assigned to seven groups. All groups, except the healthy control (GI), were infected with the\n                      <jats:italic>Toxoplasma gondii<\/jats:italic>\n                      ME49 strain. Treatments included Fe-MOFs (GIII), spiramycin (GIV), spiramycin@Fe-MOFs (GV), garlic extract (GVI), and garlic extract@Fe-MOFs (GVII). In vitro drug toxicity for garlic, Fe-MOFs, and garlic extract@Fe-MOFs were detected. Brain cysts counted, histopathological changes in various organs, and parasite DNA load (P29 gene) were assessed post-treatment using real-time PCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Spiramycin@Fe-MOFs (GV) and garlic extract @Fe-MOFs (GVII) groups showed a significant reduction in brain cyst burden (39.63% and 59.45%, respectively), along with marked improvement in histopathological changes compared to the other treated infected groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings support garlic@Fe-MOFs as a potential treatment for toxoplasmosis, demonstrating enhanced efficacy, reduced toxicity, and improved histopathological outcomes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11686-025-01142-8","type":"journal-article","created":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T16:57:27Z","timestamp":1763139447000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Therapeutic Effect of Allium sativum (Garlic) Extract Using Nanotechnology on Murine Chronic Toxoplasmosis"],"prefix":"10.1007","volume":"70","author":[{"given":"Doaa Abdulfttah Ahmad","family":"Amer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma Mohamad","family":"El-Lessy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraf M.","family":"Barakat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rehab Mohamed","family":"El Shahat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabry A.","family":"Sadek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reda M.","family":"Abdelhameed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona Mohammed","family":"Elderbawy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,11,14]]},"reference":[{"issue":"1","key":"1142_CR1","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1007\/s11011-021-00824-2","volume":"37","author":"T Nayeri","year":"2022","unstructured":"Nayeri T, Sarvi S, Daryani A (2022) Toxoplasmosis: targeting neurotransmitter systems in psychiatric disorders. 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