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This study compared 3 methods to detect babesiosis in naturally infected cattle; microscopy, loop-mediated isothermal amplification (LAMP), and conventional PCR (cPCR), in Egypt\u2019s New Valley Governorate.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 339 tick-infested cattle were examined for\n                      <jats:italic>Babesia<\/jats:italic>\n                      infection. Microscopic examination was done. Genomic DNA extraction for the LAMP and PCR assays was carried out. LAMP was performed and compared with cPCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Molecular methods revealed higher infection rates than microscopic examination: LAMP detected\n                      <jats:italic>Babesia<\/jats:italic>\n                      DNA in 40.7% (138\/339) of samples, cPCR in 41.9% (142\/339), while microscopy identified 31.9% (108\/339). Clinically infected cattle exhibited fever, hemoglobinuria, pallor, and weight loss. Seasonal variation showed peak prevalence in summer (37.8%) and the lowest in winter (20%). Based on comparative analysis, LAMP showed 97.18% sensitivity, 100% specificity, and almost perfect agreement with cPCR (\u03ba\u2009=\u20090.98,\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.13).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These findings demonstrate high\n                      <jats:italic>Babesia<\/jats:italic>\n                      prevalence in tick-infested cattle and underscore LAMP\u2019s value as a rapid, sensitive, and field-friendly tool for surveillance.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11686-025-01194-w","type":"journal-article","created":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T09:43:30Z","timestamp":1768297410000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Comparative Diagnostic Efficacy of Microscopy, LAMP and PCR for Detection of Bovine Babesiosis in New Valley Governorate, Egypt"],"prefix":"10.1007","volume":"71","author":[{"given":"Khatib H.","family":"Abdelwahab","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed E. 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