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This issue has driven research and the development of new therapeutic and preventive strategies, with a particular emphasis on vaccine design.\n                  <\/jats:p>\n                  <jats:p>Materials and methods<\/jats:p>\n                  <jats:p>\n                    In the present study, a bioinformatics approach was employed to evaluate\n                    <jats:italic>T. cruzi<\/jats:italic>\n                    glyceraldehyde-3-phosphate dehydrogenase (TcGAPDH) as a potential vaccine candidate by analysing its physicochemical and immunogenic properties.\n                  <\/jats:p>\n                  <jats:p>Results<\/jats:p>\n                  <jats:p>In this analysis, epitopes for B and T lymphocytes were predicted, and a predictive immune response determined that TcGAPDH has the capacity to induce a Th1-type response driven by IFN-\u03b3 and IL-2. Finally, molecular docking and dynamics simulation demonstrated favourable interaction between TcGAPDH and TLR2\/TLR4 receptors, which remained constant throughout a 300\u00a0ns simulation.<\/jats:p>\n                  <jats:p>Conclusions<\/jats:p>\n                  <jats:p>These findings highlight the immunogenic potential of TcGAPDH and support its feasibility as a promising candidate for the development of a vaccine against Chagas disease.<\/jats:p>","DOI":"10.1007\/s11686-026-01221-4","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T12:07:44Z","timestamp":1771243664000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Silico Design and Evaluation of TcGAPDH as a Vaccine Against Chagas Disease: A Reverse Vaccinology Approach"],"prefix":"10.1007","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3099-9685","authenticated-orcid":false,"given":"Veronica","family":"Nava-Cuamatzi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3416-1625","authenticated-orcid":false,"given":"Ricardo Enrique","family":"Buendia-Corona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8062-4160","authenticated-orcid":false,"given":"Mar\u00eda Cristina","family":"Gonz\u00e1lez-V\u00e1zquez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Lilia","family":"Cedillo-Ramirez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0162-4929","authenticated-orcid":false,"given":"Alejandro","family":"Carabarin-Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,16]]},"reference":[{"key":"1221_CR1","unstructured":"World Health Organization Control of Neglected Tropical Diseases. https:\/\/www.who.int\/teams\/control-of-neglected-tropical-diseases. 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