{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T04:01:11Z","timestamp":1781064071468,"version":"3.54.1"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"2","license":[{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T00:00:00Z","timestamp":1771891200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Parasit."],"published-print":{"date-parts":[[2026,4]]},"DOI":"10.1007\/s11686-026-01233-0","type":"journal-article","created":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T10:46:59Z","timestamp":1771930019000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Reliable and Accurate In-House kDNA-Based qPCR Assay for Detection of Leishmania DNA in Microscopy-Negative Cutaneous Leishmaniasis"],"prefix":"10.1007","volume":"71","author":[{"given":"Amirmohammad","family":"FallahiLima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahdi","family":"Fakhar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajar","family":"Ziaei Hezarjaribi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"Saberi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Maleksabet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabeeh","family":"Tabaripour","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,2,24]]},"reference":[{"issue":"1","key":"1233_CR1","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1007\/s00436-023-08043-7","volume":"123","author":"A Tom","year":"2023","unstructured":"Tom A, Kumar NP, Kumar A, Saini P (2023) Interactions between leishmania parasite and sandfly: a review. Parasitol Res 123(1):6","journal-title":"Parasitol Res"},{"key":"1233_CR2","doi-asserted-by":"publisher","first-page":"1453211","DOI":"10.3389\/fmed.2024.1453211","volume":"11","author":"S Ekemen","year":"2024","unstructured":"Ekemen S, Nalcaci M, Toz S, Sanjoba C, Demirkesen C, Cetin ED et al (2024) Diagnostic challenges in CL due to atypical leishmania infantum: pathologists\u2019 insights from re-emergence zones. Front Med 11:1453211","journal-title":"Front Med"},{"issue":"1","key":"1233_CR3","doi-asserted-by":"publisher","first-page":"412","DOI":"10.1186\/s13071-022-05524-z","volume":"15","author":"I Gow","year":"2022","unstructured":"Gow I, Smith NC, Stark D, Ellis J (2022) Laboratory diagnostics for human leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods. Parasites Vectors 15(1):412","journal-title":"Parasites Vectors"},{"issue":"6","key":"1233_CR4","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1007\/s40257-022-00726-8","volume":"23","author":"HJC de Vries","year":"2022","unstructured":"de Vries HJC, Schallig HD (2022) CL: a 2022 updated narrative review into diagnosis and management developments. Am J Clin Dermatol 23(6):823\u2013840","journal-title":"Am J Clin Dermatol"},{"key":"1233_CR5","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1051\/parasite\/2022047","volume":"29","author":"Z Nasiri","year":"2022","unstructured":"Nasiri Z, Kalantari M, Mohammadi J, Daliri S, Mehrabani D, Azizi K (2022) CL in Iran: a review of epidemiological aspects, with emphasis on molecular findings. Parasite (Paris France) 29:47","journal-title":"Parasite (Paris France)"},{"issue":"11","key":"1233_CR6","doi-asserted-by":"publisher","first-page":"1632","DOI":"10.3390\/microorganisms8111632","volume":"8","author":"JQ Reim\u00e3o","year":"2020","unstructured":"Reim\u00e3o JQ, Coser EM, Lee MR, Coelho AC (2020) Laboratory diagnosis of cutaneous and visceral leishmaniasis: current and future methods. Microorganisms 8(11):1632","journal-title":"Microorganisms"},{"issue":"1","key":"1233_CR7","first-page":"1","volume":"6","author":"M Yaghoobi-Ershadi","year":"2012","unstructured":"Yaghoobi-Ershadi M (2012) Phlebotomine sand flies (Diptera: Psychodidae) in Iran and their role on leishmania transmission. J Arthropod-Borne Dis 6(1):1","journal-title":"J Arthropod-Borne Dis"},{"issue":"1","key":"1233_CR8","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1186\/s13071-018-2859-8","volume":"11","author":"L Galluzzi","year":"2018","unstructured":"Galluzzi L, Ceccarelli M, Diotallevi A, Menotta M, Magnani M (2018) Real-time PCR applications for diagnosis of leishmaniasis. Parasit Vectors 11(1):273","journal-title":"Parasit Vectors"},{"issue":"3","key":"1233_CR9","doi-asserted-by":"publisher","first-page":"475","DOI":"10.4269\/ajtmh.1983.32.475","volume":"32","author":"JD Chulay","year":"1983","unstructured":"Chulay JD, Bryceson ADM (1983) Quantitation of amastigotes of leishmania donovani in smears of Splenic aspirates from patients with visceral leishmaniasis. Am J Trop Med Hyg 32(3):475\u2013479","journal-title":"Am J Trop Med Hyg"},{"issue":"2","key":"1233_CR10","doi-asserted-by":"publisher","first-page":"310","DOI":"10.2478\/s11686-019-00154-5","volume":"65","author":"S Asfaram","year":"2020","unstructured":"Asfaram S, Fakhar M, Mirani N, Derakhshani-Niya M, Valadan R, Ziaei Hezarjaribi H et al (2020) HRM-PCR is an accurate and sensitive technique for the diagnosis of CL as compared with conventional PCR. Acta Parasitol 65(2):310\u2013316","journal-title":"Acta Parasitol"},{"issue":"2","key":"1233_CR11","doi-asserted-by":"publisher","first-page":"111","DOI":"10.4103\/0972-9062.242557","volume":"55","author":"BR Esboei","year":"2018","unstructured":"Esboei BR, Mohebali M, Mousavi P, Fakhar M, Akhoundi B (2018) Potent antileishmanial activity of Chitosan against Iranian strain of leishmania major (MRHO\/IR\/75\/ER): in vitro: and: in vivo: assay. J Vector Borne Dis 55(2):111\u2013115","journal-title":"J Vector Borne Dis"},{"issue":"2","key":"1233_CR12","first-page":"337","volume":"8","author":"M Mohaghegh","year":"2013","unstructured":"Mohaghegh M, Fata A, Salehi G, Berenji F, Bazzaz MM, Rafatpanah H et al (2013) Molecular identification of leishmania species using samples obtained from negative stained smears. Iran J Parasitol 8(2):337\u2013341","journal-title":"Iran J Parasitol"},{"key":"1233_CR13","doi-asserted-by":"crossref","unstructured":"Allahem A, Alajmi R, Alajami MA, El-Ashram S, Bashir MA, Abdel-Gaber R (2024) Molecular detection of Leishmania\u2009species in sand flies by PCR-RFLP technique in refugee camps. Arquivo Brasileiro de Med Veterin\u00e1ria e Zootecnia 76","DOI":"10.1590\/1678-4162-13178"},{"issue":"18","key":"1233_CR14","doi-asserted-by":"publisher","first-page":"2989","DOI":"10.3390\/diagnostics13182989","volume":"13","author":"SB Piyasiri","year":"2023","unstructured":"Piyasiri SB, Dewasurendra R, Samaranayake N, Karunaweera N (2023) Diagnostic tools for CL caused by leishmania donovani: a narrative review. Diagnostics 13(18):2989","journal-title":"Diagnostics"},{"issue":"3","key":"1233_CR15","first-page":"233","volume":"16","author":"S Mohammadi-Azni","year":"2022","unstructured":"Mohammadi-Azni S, Kalantari M, Pourmohammadi B (2022) Molecular detection of leishmania infection in phlebotomine sand flies from an endemic focus of zoonotic CL in Iran. J Arthropod Borne Dis 16(3):233\u2013242","journal-title":"J Arthropod Borne Dis"},{"issue":"11","key":"1233_CR16","doi-asserted-by":"publisher","first-page":"3680","DOI":"10.1128\/JCM.01286-07","volume":"45","author":"AK Boggild","year":"2007","unstructured":"Boggild AK, Miranda-Verastegui C, Espinosa D, Arevalo J, Adaui V, Tulliano G et al (2007) Evaluation of a microculture method for isolation of leishmania parasites from cutaneous lesions of patients in Peru. J Clin Microbiol 45(11):3680\u20133684","journal-title":"J Clin Microbiol"},{"issue":"6","key":"1233_CR17","first-page":"465","volume":"65","author":"KK Dixit","year":"2020","unstructured":"Dixit KK, Singh R, Salotra P (2020) Advancement in molecular diagnosis of post Kala-Azar dermal leishmaniasis. Indian J Dermatol 65(6):465\u2013472","journal-title":"Indian J Dermatol"},{"issue":"3","key":"1233_CR18","first-page":"382","volume":"8","author":"F Kheirandish","year":"2013","unstructured":"Kheirandish F, Chegeni Sharafi A, Kazemi B, Mohebali M, Sarlak A, Tarahi MJ et al (2013) Identification of leishmania species using PCR assay on giemsa-stained slides prepared from CL patients. Iran J Parasitol 8(3):382\u2013388","journal-title":"Iran J Parasitol"},{"issue":"5","key":"1233_CR19","doi-asserted-by":"publisher","first-page":"1156","DOI":"10.4269\/ajtmh.18-0223","volume":"99","author":"MC Almaz\u00e1n","year":"2018","unstructured":"Almaz\u00e1n MC, Hoyos CL, Krolewiecki AJ, Cajal SP, Copa GN, Fleitas PE et al (2018) Molecular identification of leishmania spp. DNA from archived Giemsa-Stained slides of patients from Salta, Argentina. Am J Trop Med Hyg 99(5):1156\u20131161","journal-title":"Am J Trop Med Hyg"},{"issue":"4","key":"1233_CR20","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1007\/s12639-020-01246-0","volume":"44","author":"S Aghamolaei","year":"2020","unstructured":"Aghamolaei S, Behniafar H, Behravan M, Hajjaran H, Moin Vaziri V (2020) Probability of false-negative results in microscopical detection of CL: more accurate screening by kDNA-PCR during epidemiological survey. J Parasitic Dis Off Organ Indian Soc Parasitol 44(4):781\u2013784","journal-title":"J Parasitic Dis Off Organ Indian Soc Parasitol"},{"issue":"4","key":"1233_CR21","doi-asserted-by":"publisher","first-page":"1435","DOI":"10.1128\/JCM.44.4.1435-1439.2006","volume":"44","author":"E Bensoussan","year":"2006","unstructured":"Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL (2006) Comparison of PCR assays for diagnosis of CL. J Clin Microbiol 44(4):1435\u20131439","journal-title":"J Clin Microbiol"},{"key":"1233_CR22","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.exppara.2016.02.007","volume":"164","author":"IS Koltas","year":"2016","unstructured":"Koltas IS, Eroglu F, Uzun S, Alabaz D (2016) A comparative analysis of different molecular targets using PCR for diagnosis of old world leishmaniasis. Exp Parasitol 164:43\u201348","journal-title":"Exp Parasitol"},{"issue":"1","key":"1233_CR23","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.exppara.2012.10.017","volume":"133","author":"A Mohammadiha","year":"2013","unstructured":"Mohammadiha A, Mohebali M, Haghighi A, Mahdian R, Abadi AR, Zarei Z et al (2013) Comparison of real-time PCR and conventional PCR with two DNA targets for detection of leishmania (Leishmania) infantum infection in human and dog blood samples. Exp Parasitol 133(1):89\u201394","journal-title":"Exp Parasitol"}],"container-title":["Acta Parasitologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11686-026-01233-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11686-026-01233-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11686-026-01233-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T03:24:22Z","timestamp":1781061862000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11686-026-01233-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,24]]},"references-count":23,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,4]]}},"alternative-id":["1233"],"URL":"https:\/\/doi.org\/10.1007\/s11686-026-01233-0","relation":{},"ISSN":["1230-2821","1896-1851"],"issn-type":[{"value":"1230-2821","type":"print"},{"value":"1896-1851","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,24]]},"assertion":[{"value":"22 September 2025","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"29 October 2025","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"24 February 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Declarations"}},{"value":"This study was approved by the ethical committee at Mazandaran University of Medical Sciences (IR.MAZUMS.REC.1404.037). All participants gave written informed consent to provide skin scrapings from their lesions, which were collected for research and diagnostic purposes.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics Approval and Consent to Participate"}},{"value":"The authors declare no competing interests.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"51"}}