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In this context, microalgae biocompounds have emerged as promising sources of antiparasitic agents.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study aims to evaluate the\n                      <jats:italic>in vitro<\/jats:italic>\n                      preliminary screening activity (vital dye exclusion).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Aqueous extracts of\n                      <jats:italic>Arthrospira platensis<\/jats:italic>\n                      obtained by different extraction methods, as well as the bioactivity of the product derived from\n                      <jats:italic>in vitro<\/jats:italic>\n                      digestion (PDV), aiming to assess the influence of extraction and digestive bioactivation on antiparasitic efficacy. Extracts were produced by magnetic stirring and sonication, followed by exposure of\n                      <jats:italic>Giardia duodenalis<\/jats:italic>\n                      cysts to increasing concentrations, with metronidazole used as a reference drug. The PDV was generated using a standardized simulated gastrointestinal digestion model. Sonicated extracts showed enhanced membrane damage compared to mechanically stirred extracts, consistent with improved release of intracellular bioactive compounds. The IC\n                      <jats:sub>50<\/jats:sub>\n                      values for the sonicated, agitated, and PDV samples were, respectively: 95,4; 153.1 and 43.6\u00a0\u03bcg\/mL.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The PDV demonstrated activity across multiple concentrations and, under comparable conditions, outperformed metronidazole. These findings suggest that digestive processing may increase the bioaccessibility of active moieties responsible for antiparasitic effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Overall, the results support\n                      <jats:italic>A. platensis<\/jats:italic>\n                      as a sustainable source of giardicidal biocompounds and highlight the relevance of physiologically relevant in vitro digestion models for predicting functional antiparasitic activity and guiding future translational research.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11686-026-01313-1","type":"journal-article","created":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T08:01:15Z","timestamp":1780732875000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sustainable Production and In Vitro Giardicidal Activity of Bioactive Compounds Derived from Arthrospira Platensis"],"prefix":"10.1007","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0822-4394","authenticated-orcid":false,"given":"Ketyline Lira","family":"de Lima","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8965-5561","authenticated-orcid":false,"given":"J\u00falia Sofia Neves","family":"de Sousa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-4057-3428","authenticated-orcid":false,"given":"Karina Evelyn","family":"de Lima Albuquerque Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0512-5801","authenticated-orcid":false,"given":"Maria Rafaele","family":"Oliveira Bezerra da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1801-2945","authenticated-orcid":false,"given":"Raquel Pedrosa","family":"Bezerra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7045-2975","authenticated-orcid":false,"given":"Romero Marcos Pedrosa Brand\u00e3o","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0317-0307","authenticated-orcid":false,"given":"Kethylen Barbara Barbosa","family":"Cardoso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6960-3382","authenticated-orcid":false,"given":"Patr\u00edcia Melo","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5209-5043","authenticated-orcid":false,"given":"Gen\u00e9sio Florentino","family":"Duarte Neto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4421-6228","authenticated-orcid":false,"given":"Silvana de F\u00e1tima","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2380-7910","authenticated-orcid":false,"given":"Daniela","family":"de Ara\u00fajo Viana Marques","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,6]]},"reference":[{"key":"#cr-split#-1313_CR1.1","unstructured":"Stephen T, Tobin EH, Juergens AL, Giardiasis (2026) In StatPearls [Internet]"},{"key":"#cr-split#-1313_CR1.2","unstructured":"StatPearls Publishing: Treasure Island, FL, USA, 2025. 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