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However, data on its molecular prevalence in cattle from semiarid and transitional areas of the Northeast region of Brazil remain scarce. This study aimed to determine the prevalence of\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    spp. in cattle from smallholder farms located in the Sert\u00e3o and Agreste mesoregions of the states of Para\u00edba and Pernambuco, Brazil. A total of 380 whole blood samples from cattle were analyzed by conventional PCR targeting the\n                    <jats:italic>msp4<\/jats:italic>\n                    gene, following confirmation of DNA extraction by amplification of the endogenous\n                    <jats:italic>GAPDH<\/jats:italic>\n                    gene.\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    spp. was detected in 92.1% of the cattle, with no significant association with sex, age group, breed, production system, or level of tick infestation. Positive cattle exhibited significantly lower hematocrit values compared to negative animals (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.0087). These results represent the first report of molecular detection of\n                    <jats:italic>Anaplasma<\/jats:italic>\n                    spp. in cattle from the states of Pernambuco and Para\u00edba and suggest the presence of enzootic stability microregions in the Sert\u00e3o and Agreste mesoregions of Northeast Brazil.\n                  <\/jats:p>","DOI":"10.1007\/s11686-026-01322-0","type":"journal-article","created":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T08:01:06Z","timestamp":1780732866000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High Molecular Prevalence of Anaplasma spp. in Cattle from the Sert\u00e3o and Agreste Regions, Northeast Brazil"],"prefix":"10.1007","volume":"71","author":[{"given":"Leila Maria Rosa","family":"dos Santos","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus Dias","family":"Cordeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maiara Vasconcelos","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina de Paula","family":"de Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliano Martins","family":"Santiago","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia Bezerra","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna de Azevedo","family":"Ba\u00eata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonatas Campos","family":"de Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,6]]},"reference":[{"key":"1322_CR1","first-page":"7","volume-title":"Report of the Government on Veterinary Bacteriology in Transvaal (1908\u20131909)","author":"A Theiler","year":"1910","unstructured":"Theiler A (1910) Anaplasma marginale (gen. and spec. nov). 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