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Conventional PCR assays were performed for\n                      <jats:italic>Babesia<\/jats:italic>\n                      spp. and\n                      <jats:italic>Hepatozoon<\/jats:italic>\n                      spp., whereas nested PCR was used for\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      spp. Positive amplicons were sequenced and subjected to phylogenetic analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Five samples (4.35%) from\n                      <jats:italic>D. albiventris<\/jats:italic>\n                      collected in Botucatu were positive for\n                      <jats:italic>Babesia<\/jats:italic>\n                      spp. based on the 18\u00a0S rRNA gene, and two samples (1.74%) were positive for\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      spp. based on the\n                      <jats:italic>dsb<\/jats:italic>\n                      gene. No\n                      <jats:italic>Hepatozoon<\/jats:italic>\n                      spp. DNA was detected. Sequence analysis revealed 100% identity between the\n                      <jats:italic>Babesia<\/jats:italic>\n                      amplicons and\n                      <jats:italic>Babesia<\/jats:italic>\n                      sp. (GenBank: MW290046), while one\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      sequence showed 100% identity with\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      sp. strain Natal (GenBank: KY207546; OP270482), and the second sequence showed 99.71% identity with\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      sp. (GenBank: OP270482). Phylogenetic reconstruction clustered the\n                      <jats:italic>Babesia<\/jats:italic>\n                      sequences within the South American Marsupialia Group and positioned the\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      sequences together with lineages previously reported in South American marsupials.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These findings provide the first molecular detection of\n                      <jats:italic>Babesia<\/jats:italic>\n                      sp. and\n                      <jats:italic>Ehrlichia<\/jats:italic>\n                      sp. in free-living\n                      <jats:italic>D. albiventris<\/jats:italic>\n                      from Botucatu, S\u00e3o Paulo State, Brazil. The results expand current knowledge on vector-borne pathogens in synanthropic marsupials and reinforce the importance of continued surveillance to improve understanding of host\u2013pathogen interactions at urban\u2013wildlife interfaces within a One Health perspective.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11686-026-01325-x","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T10:07:08Z","timestamp":1781172428000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular detection and phylogenetic characterization of Babesia and Ehrlichia in free-living opossums from southeastern Brazil"],"prefix":"10.1007","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-4978-0015","authenticated-orcid":false,"given":"Stephany Rocha","family":"Ribeiro","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7901-9411","authenticated-orcid":false,"given":"Larissa Pereira","family":"Rodrigues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7824-2666","authenticated-orcid":false,"given":"Jo\u00e3o Ot\u00e1vio","family":"Mochiuti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-6577-6595","authenticated-orcid":false,"given":"Catharina Eulasya Viana","family":"Resende","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2939-5301","authenticated-orcid":false,"given":"Gilberto Salles","family":"Gazeta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4499-5401","authenticated-orcid":false,"given":"Ana Beatriz","family":"Borsoi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7101-8174","authenticated-orcid":false,"given":"Mariana Guimar\u00e3es C\u00f4rtes","family":"da Cruz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7812-9336","authenticated-orcid":false,"given":"Karla","family":"Bitencourth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8219-0845","authenticated-orcid":false,"given":"Rodrigo Hidalgo Friciello","family":"Teixeira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4513-753X","authenticated-orcid":false,"given":"L\u00edgia Souza Silveira","family":"da Lima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,6,11]]},"reference":[{"key":"1325_CR1","doi-asserted-by":"publisher","first-page":"689","DOI":"10.1016\/j.arcmed.2005.03.041","volume":"36","author":"AA Khasnis","year":"2005","unstructured":"Khasnis AA, Nettleman MD (2005) Global warming and infectious disease. 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