{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T06:05:59Z","timestamp":1789106759545,"version":"build-2803163510"},"reference-count":17,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100006416","name":"Universidade Estadual De Campinas","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006416","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Acta Parasit."],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Parasite structures eliminated in feces have chemical characteristics resulting from their interactions with the environment in which they are found. These poorly documented physicochemical conditions may interfere with separation techniques used to detect helminth eggs and larvae, as well as protozoan cysts, oocysts, and spores. This interference negatively affects diagnostic protocols for these pathogens, which are generally low to moderate in sensitivity. As such, we sought to evaluate the surface charge by zeta potential (ZP) of the species\n                      <jats:italic>Ascaris lumbricoides<\/jats:italic>\n                      ,\n                      <jats:italic>Giardia duodenalis<\/jats:italic>\n                      , and\n                      <jats:italic>Taenia<\/jats:italic>\n                      spp.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We screened fecal samples that tested positive for these parasites at high intensity. These positive samples were then filtered through tubes containing 400\u00a0\u00b5m and 200\u00a0\u00b5m meshes, after which a 500 \u00b5L aliquot was diluted with 9\u00a0mL of deionized water and centrifuged (490\u00a0g \/ 5\u00a0min). This dilution and centrifugation procedure was performed 5 times to obtain a concentrated parasite sediment, which was then transferred to Petri dishes containing 7.5% formalin in deionized water. Eggs and cysts were visualized with stereomicroscopy equipment, captured with a 10 \u00b5L micropipette, and stored in Eppendorf tubes (2\u00a0mL).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Zeta potential values were analyzed with a Zetasizer nano device, and the average data obtained from in triplicate were \u2212\u200928\u00a0mV, \u2212\u200921.9\u00a0mV, and \u2212\u200918.4\u00a0mV for\n                      <jats:italic>G. duodenalis<\/jats:italic>\n                      ,\n                      <jats:italic>A. lumbricoides<\/jats:italic>\n                      , and\n                      <jats:italic>Taenia<\/jats:italic>\n                      spp., respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We conclude that the negative charges presented on the surfaces of these parasite structures in natural fecal samples can negatively affect laboratory diagnostic techniques.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1007\/s11686-026-01367-1","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T05:13:32Z","timestamp":1789103612000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Evaluation of Surface Charges of Ascaris Lumbricoides, Giardia Duodenalis, and Taenia spp., in Preserved Fecal Samples Processed in Natura"],"prefix":"10.1007","volume":"71","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3408-2870","authenticated-orcid":false,"given":"Qu\u00e9ren Hapuque","family":"de Castro Novelli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe Augusto","family":"Soares","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vin\u00edcius Salles","family":"Margatho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico Piovesana","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celso Tetsuo Nagase","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edvaldo","family":"Sabadini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca Martins","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leyva Cec\u00edlia Vieira","family":"de Melo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda","family":"de Oliveira Baccin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandre Xavier","family":"Falc\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jancarlo Ferreira","family":"Gomes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"1367_CR1","doi-asserted-by":"publisher","DOI":"10.1016\/j.onehlt.2019.100106","volume":"9","author":"SH Tinkler","year":"2020","unstructured":"Tinkler SH (2020) Preventive chemotherapy and anthelmintic resistance of soil-transmitted helminths \u2013 can we learn nothing from veterinary medicine? One Health 9:100106. https:\/\/doi.org\/10.1016\/j.onehlt.2019.100106","journal-title":"One Health"},{"key":"1367_CR2","doi-asserted-by":"publisher","DOI":"10.1590\/0037-8682-0535-2019","volume":"53","author":"FA Soares","year":"2020","unstructured":"Soares FA et al (2020) A historical review of the techniques of recovery of parasites for their detection in human stools. Rev Soc Bras Med Trop 53:e20190535. https:\/\/doi.org\/10.1590\/0037-8682-0535-2019","journal-title":"Rev Soc Bras Med Trop"},{"issue":"11","key":"1367_CR3","doi-asserted-by":"publisher","first-page":"4051","DOI":"10.1007\/s00436-015-4632-7","volume":"114","author":"T Irie","year":"2015","unstructured":"Irie T, Yamaguchi Y, Sumen A, Habe S, Horii Y, Nonaka N (2015) Evaluation of the MGL method to detect Paragonimus eggs and its improvement. Parasitol Res 114(11):4051\u20134058. https:\/\/doi.org\/10.1007\/s00436-015-4632-7","journal-title":"Parasitol Res"},{"issue":"15","key":"1367_CR4","doi-asserted-by":"publisher","first-page":"4651","DOI":"10.1016\/j.watres.2011.06.017","volume":"45","author":"ME Sengupta","year":"2011","unstructured":"Sengupta ME, Thamsborg SM, Andersen TJ, Olsen A, Dalsgaard A (2011) Sedimentation of helminth eggs in water. Water Res 45(15):4651\u20134660. https:\/\/doi.org\/10.1016\/j.watres.2011.06.017","journal-title":"Water Res"},{"issue":"2","key":"1367_CR5","doi-asserted-by":"publisher","first-page":"e73","DOI":"10.1371\/journal.pntd.0000073","volume":"1","author":"CF Teixeira","year":"2007","unstructured":"Teixeira CF, Neuhauss E, Ben R, Romanzini J, Graeff-Teixeira C (2007) Detection of schistosoma mansoni eggs in feces through their interaction with paramagnetic beads in a magnetic field. PLoS Negl Trop Dis 1(2):e73. https:\/\/doi.org\/10.1371\/journal.pntd.0000073","journal-title":"PLoS Negl Trop Dis"},{"issue":"2","key":"1367_CR6","doi-asserted-by":"publisher","first-page":"406","DOI":"10.1645\/0022-3395(2001)087[0406:QOCPOI]2.0.CO;2","volume":"87","author":"DM Moss","year":"2001","unstructured":"Moss DM, Arrowood MJ (2001) Quantification of Cryptosporidium parvum oocysts in mouse fecal specimens using immunomagnetic particles and two-color flow cytometry. J Parasitol 87(2):406\u2013412","journal-title":"J Parasitol"},{"issue":"1","key":"1367_CR7","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1016\/j.ijpara.2014.08.011","volume":"45","author":"RRF Candido","year":"2015","unstructured":"Candido RRF et al (2015) The affinity of magnetic microspheres for Schistosoma eggs. Int J Parasitol 45(1):43\u201350. https:\/\/doi.org\/10.1016\/j.ijpara.2014.08.011","journal-title":"Int J Parasitol"},{"issue":"12","key":"1367_CR8","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1111\/tmi.13827","volume":"27","author":"FA Soares","year":"2022","unstructured":"Soares FA et al (2022) Detection of intestinal parasites in human faecal samples using dissolved air flotation. Trop Med Int Health 27(12):1044\u20131052. https:\/\/doi.org\/10.1111\/tmi.13827","journal-title":"Trop Med Int Health"},{"issue":"1","key":"1367_CR9","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1016\/S0927-7757(01)01009-3","volume":"201","author":"B-M Hsu","year":"2002","unstructured":"Hsu B-M, Huang C (2002) Influence of ionic strength and pH on hydrophobicity and zeta potential of Giardia and Cryptosporidium. Colloids Surf Physicochem Eng Asp 201(1):201\u2013206. https:\/\/doi.org\/10.1016\/S0927-7757(01)01009-3","journal-title":"Colloids Surf Physicochem Eng Asp"},{"issue":"3","key":"1367_CR10","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1061\/(ASCE)0733-9372(1996)122:3(228)","volume":"122","author":"JE Ongerth","year":"1996","unstructured":"Ongerth JE, Pecoraro JP (1996) Electrophoretic mobility of cryptosporidium oocysts and giardia cysts. J Environ Eng 122(3):228\u2013231. https:\/\/doi.org\/10.1061\/(ASCE)0733-9372(1996)122:3(228)","journal-title":"J Environ Eng"},{"issue":"2","key":"1367_CR11","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/S0927-7765(01)00145-X","volume":"22","author":"S Capizzi","year":"2001","unstructured":"Capizzi S, Schwartzbrod J (2001) Surface properties of Ascaris suum eggs: hydrophobic potential and Lewis acid\u2013base interactions. Colloids Surf B Biointerfaces 22(2):99\u2013105. https:\/\/doi.org\/10.1016\/S0927-7765(01)00145-X","journal-title":"Colloids Surf B Biointerfaces"},{"issue":"2","key":"1367_CR12","doi-asserted-by":"publisher","first-page":"97","DOI":"10.2307\/3274720","volume":"42","author":"RA Rogers","year":"1956","unstructured":"Rogers RA (1956) A study of eggs of Ascaris lumbricoides var. suum with the electron microscope. J Parasitol 42(2):97\u2013108","journal-title":"J Parasitol"},{"issue":"11","key":"1367_CR13","doi-asserted-by":"publisher","first-page":"1320","DOI":"10.1111\/tmi.13308","volume":"24","author":"SL Rosa","year":"2019","unstructured":"Rosa SL et al (2019) Use of the aqueous biphasic system as an alternative for concentration of Ascaris lumbricoides eggs, with non-toxic separation of faecal residues and fats. Trop Med Int Health 24(11):1320\u20131329. https:\/\/doi.org\/10.1111\/tmi.13308","journal-title":"Trop Med Int Health"},{"issue":"4","key":"1367_CR14","doi-asserted-by":"publisher","first-page":"e0004579","DOI":"10.1371\/journal.pntd.0004579","volume":"10","author":"MM Manser","year":"2016","unstructured":"Manser MM, Saez ACS, Chiodini PL (2016) Faecal parasitology: concentration methodology needs to be better standardised. PLoS Negl Trop Dis 10(4):e0004579. https:\/\/doi.org\/10.1371\/journal.pntd.0004579","journal-title":"PLoS Negl Trop Dis"},{"issue":"12","key":"1367_CR15","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1016\/j.pt.2015.08.007","volume":"31","author":"PA Collender","year":"2015","unstructured":"Collender PA, Kirby AE, Addiss DG, Freeman MC, Remais JV (2015) Methods for quantification of soil-transmitted helminths in environmental media: current techniques and recent advances. Trends Parasitol 31(12):625\u2013639. https:\/\/doi.org\/10.1016\/j.pt.2015.08.007","journal-title":"Trends Parasitol"},{"issue":"1","key":"1367_CR16","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00025-17","volume":"31","author":"LS Garcia","year":"2018","unstructured":"Garcia LS et al (2018) Practical guidance for clinical microbiology laboratories: laboratory diagnosis of parasites from the gastrointestinal tract. Clin Microbiol Rev 31(1):e00025-17. https:\/\/doi.org\/10.1128\/CMR.00025-17","journal-title":"Clin Microbiol Rev"},{"issue":"2","key":"1367_CR17","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.exppara.2012.03.005","volume":"131","author":"F Tokumasu","year":"2012","unstructured":"Tokumasu F, Ostera GR, Amaratunga C, Fairhurst RM (2012) Modifications in erythrocyte membrane zeta potential by Plasmodium falciparum infection. Exp Parasitol 131(2):245\u2013251. https:\/\/doi.org\/10.1016\/j.exppara.2012.03.005","journal-title":"Exp Parasitol"}],"container-title":["Acta Parasitologica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11686-026-01367-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1007\/s11686-026-01367-1","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1007\/s11686-026-01367-1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T05:13:33Z","timestamp":1789103613000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1007\/s11686-026-01367-1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,11]]},"references-count":17,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["1367"],"URL":"https:\/\/doi.org\/10.1007\/s11686-026-01367-1","relation":{},"ISSN":["1230-2821","1896-1851"],"issn-type":[{"value":"1230-2821","type":"print"},{"value":"1896-1851","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,11]]},"assertion":[{"value":"29 January 2026","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"21 July 2026","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 September 2026","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.","order":1,"name":"Ethics","label":"Conflict of interest","group":{"name":"EthicsHeading","label":"Declarations"}}],"article-number":"209"}}