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This study investigated the occurrence and genetic diversity of\n                    <jats:italic>E. canis<\/jats:italic>\n                    in naturally infected dogs from the Una\u00ed microregion, Minas Gerais, Brazil, using the\n                    <jats:italic>trp19<\/jats:italic>\n                    ,\n                    <jats:italic>p28<\/jats:italic>\n                    , and\n                    <jats:italic>trp36<\/jats:italic>\n                    genes as molecular markers. A total of 386 whole blood samples were initially screened by conventional PCR targeting the Anaplasmataceae 16S rRNA gene, yielding 38 positive samples (9.8%). Subsequent PCR targeting the\n                    <jats:italic>E. canis trp19<\/jats:italic>\n                    gene confirmed infection in 21 samples (5.4%). Among these, 12 samples were positive for the p28 gene and eight for the\n                    <jats:italic>trp36<\/jats:italic>\n                    gene. Sequence analysis of\n                    <jats:italic>p28<\/jats:italic>\n                    revealed high nucleotide identity (99.27\u201399.88%) with reference strains and amino acid substitutions. Haplotype network analysis identified nine\n                    <jats:italic>p28<\/jats:italic>\n                    haplotypes, indicating substantial intraspecific genetic diversity. Analysis of the\n                    <jats:italic>trp36<\/jats:italic>\n                    gene demonstrated the co-circulation of Brazilian and American genogroups, with variation in tandem repeat copy number. These findings reveal a genetically heterogeneous\n                    <jats:italic>E. canis<\/jats:italic>\n                    population in the Una\u00ed microregion and provide evidence of the co-circulation of distinct lineages. The combined use of multiple molecular markers improved the characterization of the circulating strains and contributes to a better understanding of the molecular epidemiology and population diversity of\n                    <jats:italic>E. canis<\/jats:italic>\n                    in Brazil.\n                  <\/jats:p>","DOI":"10.1007\/s11686-026-01389-9","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T06:16:30Z","timestamp":1788761790000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Characterization and Genetic Diversity of Ehrlichia canis in Naturally Infected Dogs from the Una\u00ed Microregion, Minas Gerais, Brazil"],"prefix":"10.1007","volume":"71","author":[{"given":"Aroldo","family":"de Souza Junior","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f4nathan David Ribas","family":"Chagas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isadora","family":"dos Santos Dias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen Meireles","family":"Brand\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiza Sena","family":"Chalub","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Marques","family":"Batista","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Oliveira","family":"Amaral","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenevaldo Barbosa","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matheus Dias","family":"Cordeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia Bezerra","family":"da Silva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0172-556X","authenticated-orcid":false,"given":"Bruna","family":"de Azevedo Ba\u00eata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2026,9,7]]},"reference":[{"issue":"10","key":"1389_CR1","doi-asserted-by":"publisher","first-page":"437","DOI":"10.1016\/j.pt.2012.07.003","volume":"28","author":"F Dantas-Torres","year":"2012","unstructured":"Dantas-Torres F, Chomel BB, Otranto D (2012) Ticks and tick-borne diseases: a One Health perspective. 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