{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:37:01Z","timestamp":1740137821783,"version":"3.37.3"},"reference-count":23,"publisher":"Springer Science and Business Media LLC","issue":"5","license":[{"start":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T00:00:00Z","timestamp":1613952000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T00:00:00Z","timestamp":1613952000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["OBES SURG"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec>\n                <jats:title>Purpose<\/jats:title>\n                <jats:p>Many studies about bariatric surgery have analyzed the effect of sleeve gastrectomy (SG) on glucose improvement, beta-cell mass, and islet size modification. The effects of SG on the other endocrine cells of the pancreas, such as the alpha-cell population, and their regulatory mechanisms remain less studied.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Materials and Methods<\/jats:title>\n                <jats:p>We focused our work on the changes in the alpha-cell population after SG in a healthy model of Wistar rats. We measured alpha-cell mass, glucose tolerance, and insulin release after oral glucose tolerance tests and plasma glucagon secretion patterns after insulin infusion. Three Wistar rat groups were employed: SG-operated, surgical control (Sham), and fasting control.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Results<\/jats:title>\n                <jats:p>The results obtained showed significant increases in the alpha-cell population after SG. The result was an increase in beta-cell transdifferentiation; it was shown by some expressed molecules (the loss of expression of Pdx-1 and the increase in Arx and Pax6 cells\/mm<jats:sup>2<\/jats:sup> of islet). The serum results were enhanced plasma glucagon secretion pattern after insulin infusion assays and normal glucose tolerance and insulin release after OGTT.<\/jats:p>\n              <\/jats:sec><jats:sec>\n                <jats:title>Conclusion<\/jats:title>\n                <jats:p>We concluded that SG leads to an expansion of the alpha-cell population, at expense of beta-cell; this expansion of alpha-cells is related to transdifferentiation. Plasma glucose level was not affected due to an increased glucagon response.<\/jats:p>\n              <\/jats:sec>","DOI":"10.1007\/s11695-021-05264-6","type":"journal-article","created":{"date-parts":[[2021,2,22]],"date-time":"2021-02-22T23:02:32Z","timestamp":1614034952000},"page":"2241-2249","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Glucagon-Producing Cell Expansion in Wistar Rats. Changes to Islet Architecture After Sleeve Gastrectomy"],"prefix":"10.1007","volume":"31","author":[{"given":"Jos\u00e9","family":"Bancalero-delosReyes","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alonso","family":"Camacho-Ram\u00edrez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9","family":"Fern\u00e1ndez-Vivero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Ribelles-Garc\u00eda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Mac\u00edas-Rodr\u00edguez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Almorza-Gomar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Carrasco-Molinillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda \u00c1ngeles","family":"Mayo-Ossorio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9-Arturo","family":"Prada-Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4138-6064","authenticated-orcid":false,"given":"Gonzalo-Mart\u00edn","family":"Perez-Arana","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2021,2,22]]},"reference":[{"issue":"12","key":"5264_CR1","doi-asserted-by":"publisher","first-page":"4600","DOI":"10.1210\/endo.141.12.7806","volume":"141","author":"R Perfetti","year":"2000","unstructured":"Perfetti R, Zhou J, Doyle ME, et al. 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All the authors declare to have read and signed the disclosure and conflict statement. We did not receive payment or services from a third party (government, commercial, private foundation, etc.) for any aspect of the submitted work (limited to the grant). We have no financial relationships (regardless of the amount of compensation) with entities that could be related to the aim of the study. We have no patents or manuscripts, pending or issued, broadly relevant to this work. There are no other relationships or activities that readers could perceive to have influenced, or that give the appearance of, or potentially influence, what we have written in the submitted work. Prior communications: We, the authors, state that the article is original, it has not been submitted for publication in another journal, and it has not yet been published either wholly or in part.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Disclosure and Conflict Statement"}},{"value":"The authors declare that ethical approval was obtained for our study. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution at which the studies were conducted, and ethical approval was obtained from the University of Cadiz Committee for the Ethical Use and Care of Experimental Animals. This committee ensured that the procedures in all experiments were performed according to international guidelines and regulations for the welfare of the animals. The internal Committee for the Ethical Use and Care of Experimental Animals followed the instructions marked for the Autonomous Andalusian Authority.","order":3,"name":"Ethics","group":{"name":"EthicsHeading","label":"Statement of Animal Welfare"}}]}}