{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:44:33Z","timestamp":1762271073780,"version":"build-2065373602"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2000,12,1]],"date-time":"2000-12-01T00:00:00Z","timestamp":975628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[2000,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>After 21 days on a diet containing 1g% cholesterol and 0.5g% cholic acid, rats had an increased content of cholesterol in liver microsomal lipids. In liver, both cholesterol content and \u03b49 desaturase activity increased, whereas \u03b46 and \u03b45 desaturase activities decreased. These changes correlated with increases in oleic, palmitoleic, and linoleic acids and decreases in arachidonic and docosahexenoic acids in total microsomal lipids. Similar fatty acid changes were found in phosphatidylcholine (PC), the principal lipid of the microsomal membrane. In PC the predominant molecular fatty acid species (67% of the total) in the control rats were 18:0\/20:4, 16:0\/20:4, and 16:0\/18:2; and they mainly determined the contribution of PC to the biophysical and biochemical properties of the phospholipid bilayer. The cholesterol diet decreased specifically the 18:0\/20:4 species, and to a lesser extent, 16:0\/20:4 and 18:0\/22:6. The 18:1\u2010containing species, especially 18:1\/18:2 and less so 16:0\/18:1 and 18:1\/20:4, were increased. A new 18:1\/18:1 species appeared. The independent effects of the presence of cholesterol and change of the fatty acid composition of the phospholipid bilayer of liver microsomes on the packing were studied by fluorescence methods using 6\u2010lauroyl\u20102,4\u2010dimethylaminonaphthalene, 1,6\u2010diphenyl\u20101,3,5\u2010hexatriene and 1\u2010(4\u2010trimethylammonium phenyl)\u20106\u2010phenyl\u20101,3,5\u2010hexatriene, which test different parameters and depths of the bilayer. Data showed that the increase of cholesterol in the membrane, and not the change of the fatty acid composition of phospholipids, was the main determinant of the increased bulk packing of the bilayer. The increase of fluid oleic\u2010 and linoleic\u2010containing species almost compensated for the drop in 20:4\u2010 and 22:6\u2010containing molecules. But the most important effect was that the general drop in essential n\u20106 and n\u20103 polyunsaturated fatty acids meant that this endogenous source for the needs of the animal decreased.<\/jats:p>","DOI":"10.1007\/s11745-000-0650-7","type":"journal-article","created":{"date-parts":[[2006,11,13]],"date-time":"2006-11-13T12:45:10Z","timestamp":1163421910000},"page":"1335-1344","source":"Crossref","is-referenced-by-count":8,"title":["Dietary cholesterol induces changes in molecular species of hepatic microsomal phosphatidylcholine"],"prefix":"10.1002","volume":"35","author":[{"given":"Ana M.","family":"Bernasconi","sequence":"first","affiliation":[{"name":"Instituto de Investigationes Bioqu\u00edmicas (INIBIOLP), CONICET\u2010UNLP Facultad de Ciencias M\u00e9dicas calles 60y 120 La Plata 1900 Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Horacio A.","family":"Garda","sequence":"additional","affiliation":[{"name":"Instituto de Investigationes Bioqu\u00edmicas (INIBIOLP), CONICET\u2010UNLP Facultad de Ciencias M\u00e9dicas calles 60y 120 La Plata 1900 Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodolfo R.","family":"Brenner","sequence":"additional","affiliation":[{"name":"Instituto de Investigationes Bioqu\u00edmicas (INIBIOLP), CONICET\u2010UNLP Facultad de Ciencias M\u00e9dicas calles 60y 120 La Plata 1900 Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,12]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(87)90052-X"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(88)90296-2"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7827(86)90131-1"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00103a015"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(86)90169-0"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2580723"},{"key":"e_1_2_2_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(85)90194-4"},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(84)90019-1"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0005-2760(97)00010-6"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/87.3.267"},{"key":"e_1_2_2_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)40969-1"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_2_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_2_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac60178a040"},{"key":"e_1_2_2_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac60119a033"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826079208017168"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)33896-7"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00570a021"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-7658-7_3"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009-3084(94)02303-4"},{"key":"e_1_2_2_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)40078-1"},{"key":"e_1_2_2_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-3084(97)00068-6"},{"key":"e_1_2_2_24_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.1673201"},{"key":"e_1_2_2_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)85502-3"},{"key":"e_1_2_2_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)86104-5"},{"key":"e_1_2_2_27_1","first-page":"173","article-title":"Limited Rotational Motions: Recognition by Differential Phase Fluorometry","volume":"54","author":"Weber G.","year":"1978","journal-title":"Acta Phys. 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