{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T14:41:51Z","timestamp":1760971311112,"version":"build-2065373602"},"reference-count":22,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2000,12,1]],"date-time":"2000-12-01T00:00:00Z","timestamp":975628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[2000,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The first peroxisome proliferator\u2010activated receptor (PPAR) was cloned in 1990 by Issemann and Green. Many studies have reported the importance of this receptor in the control of gene expression of enzymes involved in lipid metabolic pathways including mitochondrial and peroxisomal fatty acid \u03b2\u2010oxidation, lipoprotein structure [apolipoprotein (apo) A2, apo Clll], and fatty acid synthase. By using radiolabeled molecules, it was shown that peroxisome proliferators bind and activate PPAR. As an alternative method, we developed a fluorescent dansyl (1\u2010dimethyl\u2010aminonaphthalene\u20105\u2010sulfonyl) derivative peroxisome proliferator from bezafibrate (DNS\u2010X), a hypolipidemic agent that exhibits an <jats:italic>in vitro<\/jats:italic> peroxisome proliferative activity on rat Fao\u2010hepatic derived cultured cells. However, until now, the effect of this new compound on the liver of animals and subcellular localization was unknown. In addition to <jats:italic>in vivo<\/jats:italic> rat studies, we present a more efficient large\u2010scale technique of DNS\u2010X purification. Treating rats (DNS\u2010X in the diet at 0.3% w\/w) for 6 d leads to a hepatomegaly and a marked increase in liver peroxisomal palmitoyl\u2010CoA oxidase activity. We also developed a method to localize and quantify DNS\u2010X in tissues or cell compartment organelles. The primarily cytosolic distribution of DNS\u2010X was confirmed by direct visualization using fluorescence microscopy of cultured Fao cells. Finally, transfection assay demonstrated that DNS\u2010X enhanced the PPAR\u03b1 activity as well as other peroxisome proliferators do.<\/jats:p>","DOI":"10.1007\/s11745-000-0657-0","type":"journal-article","created":{"date-parts":[[2006,11,13]],"date-time":"2006-11-13T12:45:10Z","timestamp":1163421910000},"source":"Crossref","is-referenced-by-count":1,"title":["Properties of a fluorescent bezafibrate derivative (DNS\u2010X). A new tool to study peroxisome proliferation and fatty acid \u03b2\u2010oxidation"],"prefix":"10.1002","volume":"35","author":[{"given":"Jean\u2010Pierre","family":"Berlot","sequence":"first","affiliation":[{"name":"Facult\u00e9 des Sciences Gabriel Universit\u00e9 de Bourgogne, LBMC 6, Bd Grabriel Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Lutz","sequence":"additional","affiliation":[{"name":"Facult\u00e9 des Sciences Gabriel Universit\u00e9 de Bourgogne, LBMC 6, Bd Grabriel Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mutaspha Cherkaoui","family":"Malki","sequence":"additional","affiliation":[{"name":"Facult\u00e9 des Sciences Gabriel Universit\u00e9 de Bourgogne, LBMC 6, Bd Grabriel Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Val rie","family":"Nicolas\u2010Frances","sequence":"additional","affiliation":[{"name":"Facult\u00e9 des Sciences Gabriel Universit\u00e9 de Bourgogne, LBMC 6, Bd Grabriel Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brigitte","family":"Jannin","sequence":"additional","affiliation":[{"name":"Facult\u00e9 des Sciences Gabriel Universit\u00e9 de Bourgogne, LBMC 6, Bd Grabriel Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norbert","family":"Latruffe","sequence":"additional","affiliation":[{"name":"Facult\u00e9 des Sciences Gabriel Universit\u00e9 de Bourgogne, LBMC 6, Bd Grabriel Dijon 21000 France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,12]]},"reference":[{"key":"e_1_2_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(92)90554-V"},{"key":"e_1_2_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)10364-1"},{"key":"e_1_2_2_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1995.0316i.x"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1247\/csf.18.267"},{"key":"e_1_2_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(97)81507-6"},{"key":"e_1_2_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(90)90092-N"},{"key":"e_1_2_2_8_1","first-page":"121","article-title":"End\u2010Group Analysis Using Dansyl Chloride","volume":"55","author":"Gray W.R.","year":"1970","journal-title":"Methods Enzymol."},{"key":"e_1_2_2_9_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1993.1378"},{"key":"e_1_2_2_10_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0600604"},{"key":"e_1_2_2_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(86)90323-4"},{"key":"e_1_2_2_12_1","first-page":"150","article-title":"Properties of Peroxisomes from Jerboa (Jaculus orientalis)","volume":"70","author":"El Kebbaj M.S.","year":"1996","journal-title":"Eur. 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Mol. Biol."}],"container-title":["Lipids"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/pdf\/10.1007\/s11745-000-0657-0","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T14:04:39Z","timestamp":1760969079000},"score":1,"resource":{"primary":{"URL":"https:\/\/aocs.onlinelibrary.wiley.com\/doi\/10.1007\/s11745-000-0657-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,12]]},"references-count":22,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2000,12]]}},"alternative-id":["10.1007\/s11745-000-0657-0"],"URL":"https:\/\/doi.org\/10.1007\/s11745-000-0657-0","archive":["Portico"],"relation":{},"ISSN":["0024-4201","1558-9307"],"issn-type":[{"type":"print","value":"0024-4201"},{"type":"electronic","value":"1558-9307"}],"subject":[],"published":{"date-parts":[[2000,12]]},"article-number":"1397"}}