{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T15:03:53Z","timestamp":1778857433276,"version":"3.51.4"},"reference-count":9,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Lipids"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Most lipid extraction procedures [Folch, J., Lees, M., and Sloane\u2010Stanley, G.H., (1957) A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues, <jats:italic>J. Biol. Chem. 226<\/jats:italic>, 497\u2013509; Bligh, E.G., and Dyer, W.J. (1959) A Rapid Method of Total Lipid Extraction and Purification, <jats:italic>Can. J. Biochem. Physiol. 37<\/jats:italic>, 911\u2013917] employ biphasic solvent mixtures designed to dissolve the lipids in an organic phase and remove impurities in an aqueous phase. However, when applying these protocols to biological matrices such as that of the ocular lens, the formation of an emulsion layer between the organic and aqueous phases causes poor reproducibility in extraction yields and gives only a small amount of the lipid\u2010containing chloroform phase. In this study, we quantified phospholipids at each step of the Folch <jats:italic>et al.<\/jats:italic> extraction protocol and compared the yield of human and bovine lens phospholipids obtained by the Folch\u2010based approach and a novel monophasic methanol extraction method designed to circumvent the problems associated with biphasic extraction protocols. A monophasic methanol extraction coupled with <jats:sup>31<\/jats:sup>P NMR spectroscopy was found to be the simplest, quickest, and most effective method for quantifying the phospholipid content of the lens.<\/jats:p>","DOI":"10.1007\/s11745-002-1004-1","type":"journal-article","created":{"date-parts":[[2006,11,13]],"date-time":"2006-11-13T19:02:12Z","timestamp":1163444532000},"page":"1087-1092","source":"Crossref","is-referenced-by-count":31,"title":["<sup>31<\/sup>P NMR quantification and monophasic solvent purification of human and bovine lens phospholipids"],"prefix":"10.1002","volume":"37","author":[{"given":"William C.","family":"Byrdwell","sequence":"first","affiliation":[{"name":"Department of Chemistry University of Louisville Louisville Kentucky 40202"},{"name":"Department of Chemistry Florida Atlantic University Box 3091, 777 Glades Rd. Boca Raton FL 33401\u20100991"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidetoshi","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences University of Louisville 301 E. Muhammad Ali Blvd. Louisville KY 40202"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arne K.","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences University of Louisville 301 E. Muhammad Ali Blvd. Louisville KY 40202"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas","family":"Borchman","sequence":"additional","affiliation":[{"name":"The Institute of Physical and Chemical Research (Riken) 19\u20101399, Koeji, Nagamachi, Aoba\u2010Ku Sendai, Miyagi 980\u2010868 Japan"},{"name":"Department of Ophthalmology and Visual Sciences University of Louisville 301 E. Muhammad Ali Blvd. Louisville KY 40202"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. C.","family":"Yappert","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of Louisville Louisville Kentucky 40202"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daxin","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences University of Louisville 301 E. Muhammad Ali Blvd. Louisville KY 40202"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,11]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1139\/y59-099"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2760(69)90009-5"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/02681219380000521"},{"key":"e_1_2_1_6_1","first-page":"91","article-title":"Fat and Yeast Grown on Hydrocarbon Substrates II: Complete Extraction and Separation of Lipids","volume":"46","author":"Shuko N.","year":"1976","journal-title":"Ogata Igaku Kagaku Kenkyusho Kenkyu Hokoku"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02660240"},{"key":"e_1_2_1_8_1","first-page":"3938","article-title":"Regional and Age\u2010Dependent Differences in the Phospholipid Composition of Human Lens Membranes","volume":"35","author":"Borchman D.","year":"1994","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"e_1_2_1_9_1","first-page":"549","article-title":"Human Crystalline Lens Phospholipid Analysis with Age","volume":"32","author":"Merchant T.E.","year":"1991","journal-title":"Invest. Ophthalmol. Vis. 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